Development of an ELISA as a complement to MAT for swine leptospirosis diagnosis

Autores
Esteban, Micaela; Sanchez, Maria Cristina; Rodriguez, Marcelo A.; Saraullo, Vanina Rosa; Hamer, Micaela; Brihuega, Bibiana Felicitas; Samartino, Luis Ernesto; Martinez, Mara Leila
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leptospirosis is a widespread zoonotic disease caused by pathogenic spirochetes of the genus Leptospira, affecting humans and a broad range of domestic and wild animals, which act as maintenance or incidental hosts. In swine, infection is associated with reproductive losses and significant economic impact. Transmission occurs through direct or indirect contact with contaminated urine, soil, or water. In small-scale and extensive production systems, frequent exposure to mud, stagnant water, and wildlife increases the risk of infection with multiple Leptospira serovars, complicating diagnosis. Rapid and reliable diagnostic tools are therefore essential for effective surveillance and control. The aim of this study was to evaluate an enzyme-linked immunosorbent assay (ELISA) based on a bacterial sonicated antigen as a complementary method to the microscopic agglutination test (MAT). Using MAT as the reference method, a panel of swine sera was analyzed to determine diagnostic performance. The ELISA showed a sensitivity of 83% and a specificity of 96%, demonstrating good agreement with MAT. These results indicate that the assay provides acceptable diagnostic accuracy and may serve as a practical complementary tool for herd-level screening and surveillance of swine leptospirosis.
Instituto de Patobiología
Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Marcelo A. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Instituto de Investigación en Ciencias Agrarias y Veterinarias; Argentina
Fil: Samartino, Luis Ernesto. Universidad del Salvador. Instituto de Investigación en Ciencias Agrarias y Veterinarias; Argentina
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Mara Leila. Universidad del Salvador. Instituto de Investigación en Ciencias Agrarias y Veterinarias; Argentina
Fuente
Research in Veterinary Science 206 : 106170 (July 2026)
Materia
Leptospirosis
Swine
Diagnosis
ELISA
Antigens
Cerdo
Diagnóstico
Antígenos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/25743
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oai_identifier_str oai:localhost:20.500.12123/25743
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Development of an ELISA as a complement to MAT for swine leptospirosis diagnosisEsteban, MicaelaSanchez, Maria CristinaRodriguez, Marcelo A.Saraullo, Vanina RosaHamer, MicaelaBrihuega, Bibiana FelicitasSamartino, Luis ErnestoMartinez, Mara LeilaLeptospirosisSwineDiagnosisELISAAntigensCerdoDiagnósticoAntígenosLeptospirosis is a widespread zoonotic disease caused by pathogenic spirochetes of the genus Leptospira, affecting humans and a broad range of domestic and wild animals, which act as maintenance or incidental hosts. In swine, infection is associated with reproductive losses and significant economic impact. Transmission occurs through direct or indirect contact with contaminated urine, soil, or water. In small-scale and extensive production systems, frequent exposure to mud, stagnant water, and wildlife increases the risk of infection with multiple Leptospira serovars, complicating diagnosis. Rapid and reliable diagnostic tools are therefore essential for effective surveillance and control. The aim of this study was to evaluate an enzyme-linked immunosorbent assay (ELISA) based on a bacterial sonicated antigen as a complementary method to the microscopic agglutination test (MAT). Using MAT as the reference method, a panel of swine sera was analyzed to determine diagnostic performance. The ELISA showed a sensitivity of 83% and a specificity of 96%, demonstrating good agreement with MAT. These results indicate that the assay provides acceptable diagnostic accuracy and may serve as a practical complementary tool for herd-level screening and surveillance of swine leptospirosis.Instituto de PatobiologíaFil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodriguez, Marcelo A. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; ArgentinaFil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Instituto de Investigación en Ciencias Agrarias y Veterinarias; ArgentinaFil: Samartino, Luis Ernesto. Universidad del Salvador. Instituto de Investigación en Ciencias Agrarias y Veterinarias; ArgentinaFil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Martinez, Mara Leila. Universidad del Salvador. Instituto de Investigación en Ciencias Agrarias y Veterinarias; ArgentinaElsevier2026-04-10T09:57:22Z2026-04-10T09:57:22Z2026-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/25743https://www.sciencedirect.com/science/article/abs/pii/S00345288260012321532-26610034-5288https://doi.org/10.1016/j.rvsc.2026.106170Research in Veterinary Science 206 : 106170 (July 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-04-16T09:53:30Zoai:localhost:20.500.12123/25743instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-04-16 09:53:31.532INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development of an ELISA as a complement to MAT for swine leptospirosis diagnosis
title Development of an ELISA as a complement to MAT for swine leptospirosis diagnosis
spellingShingle Development of an ELISA as a complement to MAT for swine leptospirosis diagnosis
Esteban, Micaela
Leptospirosis
Swine
Diagnosis
ELISA
Antigens
Cerdo
Diagnóstico
Antígenos
title_short Development of an ELISA as a complement to MAT for swine leptospirosis diagnosis
title_full Development of an ELISA as a complement to MAT for swine leptospirosis diagnosis
title_fullStr Development of an ELISA as a complement to MAT for swine leptospirosis diagnosis
title_full_unstemmed Development of an ELISA as a complement to MAT for swine leptospirosis diagnosis
title_sort Development of an ELISA as a complement to MAT for swine leptospirosis diagnosis
dc.creator.none.fl_str_mv Esteban, Micaela
Sanchez, Maria Cristina
Rodriguez, Marcelo A.
Saraullo, Vanina Rosa
Hamer, Micaela
Brihuega, Bibiana Felicitas
Samartino, Luis Ernesto
Martinez, Mara Leila
author Esteban, Micaela
author_facet Esteban, Micaela
Sanchez, Maria Cristina
Rodriguez, Marcelo A.
Saraullo, Vanina Rosa
Hamer, Micaela
Brihuega, Bibiana Felicitas
Samartino, Luis Ernesto
Martinez, Mara Leila
author_role author
author2 Sanchez, Maria Cristina
Rodriguez, Marcelo A.
Saraullo, Vanina Rosa
Hamer, Micaela
Brihuega, Bibiana Felicitas
Samartino, Luis Ernesto
Martinez, Mara Leila
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Leptospirosis
Swine
Diagnosis
ELISA
Antigens
Cerdo
Diagnóstico
Antígenos
topic Leptospirosis
Swine
Diagnosis
ELISA
Antigens
Cerdo
Diagnóstico
Antígenos
dc.description.none.fl_txt_mv Leptospirosis is a widespread zoonotic disease caused by pathogenic spirochetes of the genus Leptospira, affecting humans and a broad range of domestic and wild animals, which act as maintenance or incidental hosts. In swine, infection is associated with reproductive losses and significant economic impact. Transmission occurs through direct or indirect contact with contaminated urine, soil, or water. In small-scale and extensive production systems, frequent exposure to mud, stagnant water, and wildlife increases the risk of infection with multiple Leptospira serovars, complicating diagnosis. Rapid and reliable diagnostic tools are therefore essential for effective surveillance and control. The aim of this study was to evaluate an enzyme-linked immunosorbent assay (ELISA) based on a bacterial sonicated antigen as a complementary method to the microscopic agglutination test (MAT). Using MAT as the reference method, a panel of swine sera was analyzed to determine diagnostic performance. The ELISA showed a sensitivity of 83% and a specificity of 96%, demonstrating good agreement with MAT. These results indicate that the assay provides acceptable diagnostic accuracy and may serve as a practical complementary tool for herd-level screening and surveillance of swine leptospirosis.
Instituto de Patobiología
Fil: Esteban, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Esteban, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Sanchez, Maria Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Sanchez, Maria Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Marcelo A. Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán”. Instituto Nacional de Enfermedades Infecciosas; Argentina
Fil: Saraullo, Vanina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Saraullo, Vanina Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hamer, Micaela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Hamer, Micaela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Brihuega, Bibiana Felicitas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Brihuega, Bibiana Felicitas. Universidad del Salvador. Instituto de Investigación en Ciencias Agrarias y Veterinarias; Argentina
Fil: Samartino, Luis Ernesto. Universidad del Salvador. Instituto de Investigación en Ciencias Agrarias y Veterinarias; Argentina
Fil: Martinez, Mara Leila. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Martinez, Mara Leila. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Martinez, Mara Leila. Universidad del Salvador. Instituto de Investigación en Ciencias Agrarias y Veterinarias; Argentina
description Leptospirosis is a widespread zoonotic disease caused by pathogenic spirochetes of the genus Leptospira, affecting humans and a broad range of domestic and wild animals, which act as maintenance or incidental hosts. In swine, infection is associated with reproductive losses and significant economic impact. Transmission occurs through direct or indirect contact with contaminated urine, soil, or water. In small-scale and extensive production systems, frequent exposure to mud, stagnant water, and wildlife increases the risk of infection with multiple Leptospira serovars, complicating diagnosis. Rapid and reliable diagnostic tools are therefore essential for effective surveillance and control. The aim of this study was to evaluate an enzyme-linked immunosorbent assay (ELISA) based on a bacterial sonicated antigen as a complementary method to the microscopic agglutination test (MAT). Using MAT as the reference method, a panel of swine sera was analyzed to determine diagnostic performance. The ELISA showed a sensitivity of 83% and a specificity of 96%, demonstrating good agreement with MAT. These results indicate that the assay provides acceptable diagnostic accuracy and may serve as a practical complementary tool for herd-level screening and surveillance of swine leptospirosis.
publishDate 2026
dc.date.none.fl_str_mv 2026-04-10T09:57:22Z
2026-04-10T09:57:22Z
2026-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/25743
https://www.sciencedirect.com/science/article/abs/pii/S0034528826001232
1532-2661
0034-5288
https://doi.org/10.1016/j.rvsc.2026.106170
url http://hdl.handle.net/20.500.12123/25743
https://www.sciencedirect.com/science/article/abs/pii/S0034528826001232
https://doi.org/10.1016/j.rvsc.2026.106170
identifier_str_mv 1532-2661
0034-5288
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Research in Veterinary Science 206 : 106170 (July 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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