Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples
- Autores
- Pluta, Aneta; Jaworski, Juan Pablo; Droscha, Casey; VanderWeele, Sophie; Taxis, Tasia M.; Valas, Stephen; Brnić, Dragan; Jungić, Andreja; Ruano, María José; Sánchez, Azucena; Murakami, Kenji; Nakamura, Kurumi; Puentes, Rodrigo; De Brun, María L.; Ruiz, Vanesa; Ladera Gómez, Marla Eliana; Lendez, Pamela Anahí; Dolcini, Guillermina Laura; Fernandes Camargos, Marcelo; Fonseca, Antonio; Barua, Subarna; Wang, Chengming; Giza, Aleksandra; Kuźmak, Jacek
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis and causes a persistent infection that can leave cattle with no symptoms. Many countries have been able to successfully eradicate BLV through improved detection and management methods. However, with the increasing novel molecular detection methods there have been few efforts to standardize these results at global scale. This study aimed to determine the interlaboratory accuracy and agreement of 11 molecular tests in detecting BLV. Each qPCR/ddPCR method varied by target gene, primer design, DNA input and chemistries. DNA samples were extracted from blood of BLV-seropositive cattle and lyophilized to grant a better preservation during shipping to all participants around the globe. Twenty nine out of 44 samples were correctly identified by the 11 labs and all methods exhibited a diagnostic sensitivity between 74 and 100%. Agreement amongst different assays was linked to BLV copy numbers present in samples and the characteristics of each assay (i.e., BLV target sequence). Finally, the mean correlation value for all assays was within the range of strong correlation. This study highlights the importance of continuous need for standardization and harmonization amongst assays and the different participants. The results underscore the need of an international calibrator to estimate the efficiency (standard curve) of the different assays and improve quantitation accuracy. Additionally, this will inform future participants about the variability associated with emerging chemistries, methods, and technologies used to study BLV. Altogether, by improving tests performance worldwide it will positively aid in the eradication efforts.
Instituto de Virología
Fil: Pluta, Aneta. National Veterinary Research Institute. Department of Biochemistry; Polonia
Fil: Pluta, Aneta. National Veterinary Research Institute. Department of Omics Analyses; Polonia
Fil: Jaworski, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Jaworski, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Droscha, Casey. CentralStar Cooperative; Estados Unidos
Fil: VanderWeele, Sophie. CentralStar Cooperative; Estados Unidos
Fil: Taxis, Tasia M. Michigan State University. College of Agriculture and Natural Resources. Department of Animal Science; Estados Unidos
Fil: Valas, Stephen. French Agency for Food, Environmental and Occupational Health and Safety. Unit Pathology and Welfare of Ruminants. Niort Laboratory; Francia
Fil: Brnić, Dragan. Croatian Veterinary Institute; Croacia
Fil: Jungić, Andreja. Croatian Veterinary Institute; Croacia
Fil: Ruano, María José. Ministry of Agriculture, Fisheries and Food. Laboratorio Central de Veterinaria; España
Fil: Sánchez, Azucena. Ministry of Agriculture, Fisheries and Food. Laboratorio Central de Veterinaria; España
Fil: Murakami, Kenji. Iwate University. Faculty of Agriculture. Department of Veterinary Sciences; Japón
Fil: Nakamura, Kurumi. Iwate University. Faculty of Agriculture. Department of Veterinary Sciences; Japón
Fil: Puentes, Rodrigo. Universidad de la República. Unidad de Microbiología. Facultad de Veterinaria. Departamento de Patobiología; Uruguay
Fil: De Brun, María L. Universidad de la República. Unidad de Microbiología. Facultad de Veterinaria. Departamento de Patobiología; Uruguay
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina
Fil: Ruiz, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ladera Gómez, Marla Eliana. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Departamento SAMP. Laboratorio de Virología; Argentina
Fil: Ladera Gómez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ladera Gómez, Marla Eliana. Comisión de Investigaciones Científicas de la provincia de Buenos Aires (CICPBA); Argentina
Fil: Lendez, Pamela Anahí. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Departamento SAMP. Laboratorio de Virología; Argentina
Fil: Lendez, Pamela Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lendez, Pamela Anahí. Comisión de Investigaciones Científicas de la provincia de Buenos Aires (CICPBA); Argentina
Fil: Dolcini, Guillermina Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Departamento SAMP. Laboratorio de Virología; Argentina
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dolcini, Guillermina Laura. Comisión de Investigaciones Científicas de la provincia de Buenos Aires (CICPBA); Argentina
Fil: Fernandes Camargos, Marcelo. Laboratório Federal de Defesa Agropecuária de Minas Gerais; Brasil
Fil: Fonseca, Antonio. Laboratório Federal de Defesa Agropecuária de Minas Gerais; Brasil
Fil: Barua, Subarna. Auburn University. College of Veterinary Medicine. Department of Pathobiology; Estados Unidos
Fil: Wang, Chengming. Auburn University. College of Veterinary Medicine. Department of Pathobiology; Estados Unidos
Fil: Giza, Aleksandra. National Veterinary Research Institute. Department of Omics Analyses; Polonia
Fil: Kuźmak, Jacek. National Veterinary Research Institute. Department of Biochemistry; Polonia - Fuente
- BMC Veterinary Research 20 : 381 (August 2024)
- Materia
-
Bovine Leukemia Virus
Quantitative Polymerase Chain Reaction
PCR
Virus Leucemia Bovina
Reacción en Cadena de Polimerasa Cuantitativa - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/21479
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Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samplesPluta, AnetaJaworski, Juan PabloDroscha, CaseyVanderWeele, SophieTaxis, Tasia M.Valas, StephenBrnić, DraganJungić, AndrejaRuano, María JoséSánchez, AzucenaMurakami, KenjiNakamura, KurumiPuentes, RodrigoDe Brun, María L.Ruiz, VanesaLadera Gómez, Marla ElianaLendez, Pamela AnahíDolcini, Guillermina LauraFernandes Camargos, MarceloFonseca, AntonioBarua, SubarnaWang, ChengmingGiza, AleksandraKuźmak, JacekBovine Leukemia VirusQuantitative Polymerase Chain ReactionPCRVirus Leucemia BovinaReacción en Cadena de Polimerasa CuantitativaBovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis and causes a persistent infection that can leave cattle with no symptoms. Many countries have been able to successfully eradicate BLV through improved detection and management methods. However, with the increasing novel molecular detection methods there have been few efforts to standardize these results at global scale. This study aimed to determine the interlaboratory accuracy and agreement of 11 molecular tests in detecting BLV. Each qPCR/ddPCR method varied by target gene, primer design, DNA input and chemistries. DNA samples were extracted from blood of BLV-seropositive cattle and lyophilized to grant a better preservation during shipping to all participants around the globe. Twenty nine out of 44 samples were correctly identified by the 11 labs and all methods exhibited a diagnostic sensitivity between 74 and 100%. Agreement amongst different assays was linked to BLV copy numbers present in samples and the characteristics of each assay (i.e., BLV target sequence). Finally, the mean correlation value for all assays was within the range of strong correlation. This study highlights the importance of continuous need for standardization and harmonization amongst assays and the different participants. The results underscore the need of an international calibrator to estimate the efficiency (standard curve) of the different assays and improve quantitation accuracy. Additionally, this will inform future participants about the variability associated with emerging chemistries, methods, and technologies used to study BLV. Altogether, by improving tests performance worldwide it will positively aid in the eradication efforts.Instituto de VirologíaFil: Pluta, Aneta. National Veterinary Research Institute. Department of Biochemistry; PoloniaFil: Pluta, Aneta. National Veterinary Research Institute. Department of Omics Analyses; PoloniaFil: Jaworski, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); ArgentinaFil: Jaworski, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Droscha, Casey. CentralStar Cooperative; Estados UnidosFil: VanderWeele, Sophie. CentralStar Cooperative; Estados UnidosFil: Taxis, Tasia M. Michigan State University. College of Agriculture and Natural Resources. Department of Animal Science; Estados UnidosFil: Valas, Stephen. French Agency for Food, Environmental and Occupational Health and Safety. Unit Pathology and Welfare of Ruminants. Niort Laboratory; FranciaFil: Brnić, Dragan. Croatian Veterinary Institute; CroaciaFil: Jungić, Andreja. Croatian Veterinary Institute; CroaciaFil: Ruano, María José. Ministry of Agriculture, Fisheries and Food. Laboratorio Central de Veterinaria; EspañaFil: Sánchez, Azucena. Ministry of Agriculture, Fisheries and Food. Laboratorio Central de Veterinaria; EspañaFil: Murakami, Kenji. Iwate University. Faculty of Agriculture. Department of Veterinary Sciences; JapónFil: Nakamura, Kurumi. Iwate University. Faculty of Agriculture. Department of Veterinary Sciences; JapónFil: Puentes, Rodrigo. Universidad de la República. Unidad de Microbiología. Facultad de Veterinaria. Departamento de Patobiología; UruguayFil: De Brun, María L. Universidad de la República. Unidad de Microbiología. Facultad de Veterinaria. Departamento de Patobiología; UruguayFil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); ArgentinaFil: Ruiz, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ladera Gómez, Marla Eliana. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Departamento SAMP. Laboratorio de Virología; ArgentinaFil: Ladera Gómez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ladera Gómez, Marla Eliana. Comisión de Investigaciones Científicas de la provincia de Buenos Aires (CICPBA); ArgentinaFil: Lendez, Pamela Anahí. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Departamento SAMP. Laboratorio de Virología; ArgentinaFil: Lendez, Pamela Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lendez, Pamela Anahí. Comisión de Investigaciones Científicas de la provincia de Buenos Aires (CICPBA); ArgentinaFil: Dolcini, Guillermina Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Departamento SAMP. Laboratorio de Virología; ArgentinaFil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dolcini, Guillermina Laura. Comisión de Investigaciones Científicas de la provincia de Buenos Aires (CICPBA); ArgentinaFil: Fernandes Camargos, Marcelo. Laboratório Federal de Defesa Agropecuária de Minas Gerais; BrasilFil: Fonseca, Antonio. Laboratório Federal de Defesa Agropecuária de Minas Gerais; BrasilFil: Barua, Subarna. Auburn University. College of Veterinary Medicine. Department of Pathobiology; Estados UnidosFil: Wang, Chengming. Auburn University. College of Veterinary Medicine. Department of Pathobiology; Estados UnidosFil: Giza, Aleksandra. National Veterinary Research Institute. Department of Omics Analyses; PoloniaFil: Kuźmak, Jacek. National Veterinary Research Institute. Department of Biochemistry; PoloniaBioMed Central2025-02-27T10:00:08Z2025-02-27T10:00:08Z2024-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21479https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-024-04228-z1746-6148https://doi.org/10.1186/s12917-024-04228-zBMC Veterinary Research 20 : 381 (August 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:32:10Zoai:localhost:20.500.12123/21479instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:32:11.329INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples |
| title |
Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples |
| spellingShingle |
Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples Pluta, Aneta Bovine Leukemia Virus Quantitative Polymerase Chain Reaction PCR Virus Leucemia Bovina Reacción en Cadena de Polimerasa Cuantitativa |
| title_short |
Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples |
| title_full |
Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples |
| title_fullStr |
Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples |
| title_full_unstemmed |
Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples |
| title_sort |
Inter-laboratory comparison of eleven quantitative or digital PCR assays for detection of proviral bovine leukemia virus in blood samples |
| dc.creator.none.fl_str_mv |
Pluta, Aneta Jaworski, Juan Pablo Droscha, Casey VanderWeele, Sophie Taxis, Tasia M. Valas, Stephen Brnić, Dragan Jungić, Andreja Ruano, María José Sánchez, Azucena Murakami, Kenji Nakamura, Kurumi Puentes, Rodrigo De Brun, María L. Ruiz, Vanesa Ladera Gómez, Marla Eliana Lendez, Pamela Anahí Dolcini, Guillermina Laura Fernandes Camargos, Marcelo Fonseca, Antonio Barua, Subarna Wang, Chengming Giza, Aleksandra Kuźmak, Jacek |
| author |
Pluta, Aneta |
| author_facet |
Pluta, Aneta Jaworski, Juan Pablo Droscha, Casey VanderWeele, Sophie Taxis, Tasia M. Valas, Stephen Brnić, Dragan Jungić, Andreja Ruano, María José Sánchez, Azucena Murakami, Kenji Nakamura, Kurumi Puentes, Rodrigo De Brun, María L. Ruiz, Vanesa Ladera Gómez, Marla Eliana Lendez, Pamela Anahí Dolcini, Guillermina Laura Fernandes Camargos, Marcelo Fonseca, Antonio Barua, Subarna Wang, Chengming Giza, Aleksandra Kuźmak, Jacek |
| author_role |
author |
| author2 |
Jaworski, Juan Pablo Droscha, Casey VanderWeele, Sophie Taxis, Tasia M. Valas, Stephen Brnić, Dragan Jungić, Andreja Ruano, María José Sánchez, Azucena Murakami, Kenji Nakamura, Kurumi Puentes, Rodrigo De Brun, María L. Ruiz, Vanesa Ladera Gómez, Marla Eliana Lendez, Pamela Anahí Dolcini, Guillermina Laura Fernandes Camargos, Marcelo Fonseca, Antonio Barua, Subarna Wang, Chengming Giza, Aleksandra Kuźmak, Jacek |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Bovine Leukemia Virus Quantitative Polymerase Chain Reaction PCR Virus Leucemia Bovina Reacción en Cadena de Polimerasa Cuantitativa |
| topic |
Bovine Leukemia Virus Quantitative Polymerase Chain Reaction PCR Virus Leucemia Bovina Reacción en Cadena de Polimerasa Cuantitativa |
| dc.description.none.fl_txt_mv |
Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis and causes a persistent infection that can leave cattle with no symptoms. Many countries have been able to successfully eradicate BLV through improved detection and management methods. However, with the increasing novel molecular detection methods there have been few efforts to standardize these results at global scale. This study aimed to determine the interlaboratory accuracy and agreement of 11 molecular tests in detecting BLV. Each qPCR/ddPCR method varied by target gene, primer design, DNA input and chemistries. DNA samples were extracted from blood of BLV-seropositive cattle and lyophilized to grant a better preservation during shipping to all participants around the globe. Twenty nine out of 44 samples were correctly identified by the 11 labs and all methods exhibited a diagnostic sensitivity between 74 and 100%. Agreement amongst different assays was linked to BLV copy numbers present in samples and the characteristics of each assay (i.e., BLV target sequence). Finally, the mean correlation value for all assays was within the range of strong correlation. This study highlights the importance of continuous need for standardization and harmonization amongst assays and the different participants. The results underscore the need of an international calibrator to estimate the efficiency (standard curve) of the different assays and improve quantitation accuracy. Additionally, this will inform future participants about the variability associated with emerging chemistries, methods, and technologies used to study BLV. Altogether, by improving tests performance worldwide it will positively aid in the eradication efforts. Instituto de Virología Fil: Pluta, Aneta. National Veterinary Research Institute. Department of Biochemistry; Polonia Fil: Pluta, Aneta. National Veterinary Research Institute. Department of Omics Analyses; Polonia Fil: Jaworski, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina Fil: Jaworski, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Droscha, Casey. CentralStar Cooperative; Estados Unidos Fil: VanderWeele, Sophie. CentralStar Cooperative; Estados Unidos Fil: Taxis, Tasia M. Michigan State University. College of Agriculture and Natural Resources. Department of Animal Science; Estados Unidos Fil: Valas, Stephen. French Agency for Food, Environmental and Occupational Health and Safety. Unit Pathology and Welfare of Ruminants. Niort Laboratory; Francia Fil: Brnić, Dragan. Croatian Veterinary Institute; Croacia Fil: Jungić, Andreja. Croatian Veterinary Institute; Croacia Fil: Ruano, María José. Ministry of Agriculture, Fisheries and Food. Laboratorio Central de Veterinaria; España Fil: Sánchez, Azucena. Ministry of Agriculture, Fisheries and Food. Laboratorio Central de Veterinaria; España Fil: Murakami, Kenji. Iwate University. Faculty of Agriculture. Department of Veterinary Sciences; Japón Fil: Nakamura, Kurumi. Iwate University. Faculty of Agriculture. Department of Veterinary Sciences; Japón Fil: Puentes, Rodrigo. Universidad de la República. Unidad de Microbiología. Facultad de Veterinaria. Departamento de Patobiología; Uruguay Fil: De Brun, María L. Universidad de la República. Unidad de Microbiología. Facultad de Veterinaria. Departamento de Patobiología; Uruguay Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas (IVIT); Argentina Fil: Ruiz, Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ladera Gómez, Marla Eliana. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Departamento SAMP. Laboratorio de Virología; Argentina Fil: Ladera Gómez, Marla Eliana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ladera Gómez, Marla Eliana. Comisión de Investigaciones Científicas de la provincia de Buenos Aires (CICPBA); Argentina Fil: Lendez, Pamela Anahí. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Departamento SAMP. Laboratorio de Virología; Argentina Fil: Lendez, Pamela Anahí. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Lendez, Pamela Anahí. Comisión de Investigaciones Científicas de la provincia de Buenos Aires (CICPBA); Argentina Fil: Dolcini, Guillermina Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil (CIVETAN). Departamento SAMP. Laboratorio de Virología; Argentina Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dolcini, Guillermina Laura. Comisión de Investigaciones Científicas de la provincia de Buenos Aires (CICPBA); Argentina Fil: Fernandes Camargos, Marcelo. Laboratório Federal de Defesa Agropecuária de Minas Gerais; Brasil Fil: Fonseca, Antonio. Laboratório Federal de Defesa Agropecuária de Minas Gerais; Brasil Fil: Barua, Subarna. Auburn University. College of Veterinary Medicine. Department of Pathobiology; Estados Unidos Fil: Wang, Chengming. Auburn University. College of Veterinary Medicine. Department of Pathobiology; Estados Unidos Fil: Giza, Aleksandra. National Veterinary Research Institute. Department of Omics Analyses; Polonia Fil: Kuźmak, Jacek. National Veterinary Research Institute. Department of Biochemistry; Polonia |
| description |
Bovine leukemia virus (BLV) is the etiological agent of enzootic bovine leukosis and causes a persistent infection that can leave cattle with no symptoms. Many countries have been able to successfully eradicate BLV through improved detection and management methods. However, with the increasing novel molecular detection methods there have been few efforts to standardize these results at global scale. This study aimed to determine the interlaboratory accuracy and agreement of 11 molecular tests in detecting BLV. Each qPCR/ddPCR method varied by target gene, primer design, DNA input and chemistries. DNA samples were extracted from blood of BLV-seropositive cattle and lyophilized to grant a better preservation during shipping to all participants around the globe. Twenty nine out of 44 samples were correctly identified by the 11 labs and all methods exhibited a diagnostic sensitivity between 74 and 100%. Agreement amongst different assays was linked to BLV copy numbers present in samples and the characteristics of each assay (i.e., BLV target sequence). Finally, the mean correlation value for all assays was within the range of strong correlation. This study highlights the importance of continuous need for standardization and harmonization amongst assays and the different participants. The results underscore the need of an international calibrator to estimate the efficiency (standard curve) of the different assays and improve quantitation accuracy. Additionally, this will inform future participants about the variability associated with emerging chemistries, methods, and technologies used to study BLV. Altogether, by improving tests performance worldwide it will positively aid in the eradication efforts. |
| publishDate |
2024 |
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2024-08 2025-02-27T10:00:08Z 2025-02-27T10:00:08Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/20.500.12123/21479 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-024-04228-z 1746-6148 https://doi.org/10.1186/s12917-024-04228-z |
| url |
http://hdl.handle.net/20.500.12123/21479 https://bmcvetres.biomedcentral.com/articles/10.1186/s12917-024-04228-z https://doi.org/10.1186/s12917-024-04228-z |
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eng |
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BioMed Central |
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BioMed Central |
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