An efficient vaccine against bovine leukemia virus

Autores
Gutierrez, Gerónimo; Rodríguez, Sabrina M.; Vilor, Agustín; Gillet, Nicolas A.; DeBrogniez, Alix; Trono, Karina Gabriela; Willems, Luc
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
17th International Conference on Human Retroviruses: HTLV and Related Viruses, Trois Ilets, Martinique,(FWI). 18-21 June 2015
Previous attempts to produce a vaccine against BLV faced problems of efficacy (i.e. only a fraction of animals were protected), persistence (i.e. rapid decrease of immune protection), cost (e.g. production of purified proteins) or safety (e.g. genetically modified hybrid viruses). We have designed a novel strategy based on the use of a live-attenuated BLV provirus. The rationale behind this strategy relies on the deletion of genes required to induce pathogenesis maintaining integrity of those involved in infectivity. We have identified a BLV deleted provirus that is infectious in cattle but replicates at reduced levels in cows as shown by real-time quantitative PCR. The deletant elicits a strong anti-BLV immune response as indicated by wild-type antibody titers. Vaccinated animals but not uninfected controls resist challenge by a wild type BLV virus. The deletant does not spread to uninfected sentinels maintained during 5 years in the same herd supporting biosafety of the vaccine. Passive immunity, but not viral infection, is transmitted to the newborn calves via the maternal colostrum. Assays regarding production, storage and delivery of the vaccine, as well as safety of the milk produced by vaccinated cows are currently being carried out with the aim to start a large scale trial that will be held in Argentina in real dairy conditions during this year.
Instituto de Virología
Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rodríguez, Sabrina M. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica
Fil: Vilor, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gillet, Nicolas A. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Belgica
Fil: DeBrogniez, Alix. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Belgica
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Willems, Luc. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Belgica
Fuente
Retrovirology 12 (Suppl 1) : P3. (2015)
Materia
Leucemia
Leukaemia
Virus Leucemia Bovina
Bovine Leukaemia Virus
Vacuna
Vaccines
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4477
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network_name_str INTA Digital (INTA)
spelling An efficient vaccine against bovine leukemia virusGutierrez, GerónimoRodríguez, Sabrina M.Vilor, AgustínGillet, Nicolas A.DeBrogniez, AlixTrono, Karina GabrielaWillems, LucLeucemiaLeukaemiaVirus Leucemia BovinaBovine Leukaemia VirusVacunaVaccines17th International Conference on Human Retroviruses: HTLV and Related Viruses, Trois Ilets, Martinique,(FWI). 18-21 June 2015Previous attempts to produce a vaccine against BLV faced problems of efficacy (i.e. only a fraction of animals were protected), persistence (i.e. rapid decrease of immune protection), cost (e.g. production of purified proteins) or safety (e.g. genetically modified hybrid viruses). We have designed a novel strategy based on the use of a live-attenuated BLV provirus. The rationale behind this strategy relies on the deletion of genes required to induce pathogenesis maintaining integrity of those involved in infectivity. We have identified a BLV deleted provirus that is infectious in cattle but replicates at reduced levels in cows as shown by real-time quantitative PCR. The deletant elicits a strong anti-BLV immune response as indicated by wild-type antibody titers. Vaccinated animals but not uninfected controls resist challenge by a wild type BLV virus. The deletant does not spread to uninfected sentinels maintained during 5 years in the same herd supporting biosafety of the vaccine. Passive immunity, but not viral infection, is transmitted to the newborn calves via the maternal colostrum. Assays regarding production, storage and delivery of the vaccine, as well as safety of the milk produced by vaccinated cows are currently being carried out with the aim to start a large scale trial that will be held in Argentina in real dairy conditions during this year.Instituto de VirologíaFil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Rodríguez, Sabrina M. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; BelgicaFil: Vilor, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Gillet, Nicolas A. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; BelgicaFil: DeBrogniez, Alix. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; BelgicaFil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Willems, Luc. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; BelgicaBioMed Central2019-02-20T18:14:10Z2019-02-20T18:14:10Z2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4477https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578685/1742-469010.1186/1742-4690-12-S1-I1Retrovirology 12 (Suppl 1) : P3. (2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:47:48Zoai:localhost:20.500.12123/4477instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:50.354INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv An efficient vaccine against bovine leukemia virus
title An efficient vaccine against bovine leukemia virus
spellingShingle An efficient vaccine against bovine leukemia virus
Gutierrez, Gerónimo
Leucemia
Leukaemia
Virus Leucemia Bovina
Bovine Leukaemia Virus
Vacuna
Vaccines
title_short An efficient vaccine against bovine leukemia virus
title_full An efficient vaccine against bovine leukemia virus
title_fullStr An efficient vaccine against bovine leukemia virus
title_full_unstemmed An efficient vaccine against bovine leukemia virus
title_sort An efficient vaccine against bovine leukemia virus
dc.creator.none.fl_str_mv Gutierrez, Gerónimo
Rodríguez, Sabrina M.
Vilor, Agustín
Gillet, Nicolas A.
DeBrogniez, Alix
Trono, Karina Gabriela
Willems, Luc
author Gutierrez, Gerónimo
author_facet Gutierrez, Gerónimo
Rodríguez, Sabrina M.
Vilor, Agustín
Gillet, Nicolas A.
DeBrogniez, Alix
Trono, Karina Gabriela
Willems, Luc
author_role author
author2 Rodríguez, Sabrina M.
Vilor, Agustín
Gillet, Nicolas A.
DeBrogniez, Alix
Trono, Karina Gabriela
Willems, Luc
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Leucemia
Leukaemia
Virus Leucemia Bovina
Bovine Leukaemia Virus
Vacuna
Vaccines
topic Leucemia
Leukaemia
Virus Leucemia Bovina
Bovine Leukaemia Virus
Vacuna
Vaccines
dc.description.none.fl_txt_mv 17th International Conference on Human Retroviruses: HTLV and Related Viruses, Trois Ilets, Martinique,(FWI). 18-21 June 2015
Previous attempts to produce a vaccine against BLV faced problems of efficacy (i.e. only a fraction of animals were protected), persistence (i.e. rapid decrease of immune protection), cost (e.g. production of purified proteins) or safety (e.g. genetically modified hybrid viruses). We have designed a novel strategy based on the use of a live-attenuated BLV provirus. The rationale behind this strategy relies on the deletion of genes required to induce pathogenesis maintaining integrity of those involved in infectivity. We have identified a BLV deleted provirus that is infectious in cattle but replicates at reduced levels in cows as shown by real-time quantitative PCR. The deletant elicits a strong anti-BLV immune response as indicated by wild-type antibody titers. Vaccinated animals but not uninfected controls resist challenge by a wild type BLV virus. The deletant does not spread to uninfected sentinels maintained during 5 years in the same herd supporting biosafety of the vaccine. Passive immunity, but not viral infection, is transmitted to the newborn calves via the maternal colostrum. Assays regarding production, storage and delivery of the vaccine, as well as safety of the milk produced by vaccinated cows are currently being carried out with the aim to start a large scale trial that will be held in Argentina in real dairy conditions during this year.
Instituto de Virología
Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rodríguez, Sabrina M. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica
Fil: Vilor, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gillet, Nicolas A. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Belgica
Fil: DeBrogniez, Alix. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Belgica
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Willems, Luc. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Belgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Belgica
description 17th International Conference on Human Retroviruses: HTLV and Related Viruses, Trois Ilets, Martinique,(FWI). 18-21 June 2015
publishDate 2015
dc.date.none.fl_str_mv 2015
2019-02-20T18:14:10Z
2019-02-20T18:14:10Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4477
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578685/
1742-4690
10.1186/1742-4690-12-S1-I1
url http://hdl.handle.net/20.500.12123/4477
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4578685/
identifier_str_mv 1742-4690
10.1186/1742-4690-12-S1-I1
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv Retrovirology 12 (Suppl 1) : P3. (2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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