First report of raspberry bushy dwarf virus infecting raspberry in Argentina

Autores
Dal Zotto, Angelica; Cardozo, Andrea; Cabrera Mederos, Dariel; Nome Docampo, Claudia; Giolitti, Fabian; Cobelo, Claudia Monica
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in commercial fields in parallel 42° in the Patagonia region of Argentina. Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA (Ellis et al., 2005; Martin et al., 2013). A survey was conducted to determine if RBDV was also present in Argentinian raspberry fields. Transmission electron microscope observations of leaf dip preparations revealed the presence of spherical particles of 30 nm, resembling those of virus members of the genus Ideaovirus. RBDV was detected in 88 of the 130 plants (68%) tested using double-antibody sandwich ELISA with a specific polyclonal antiserum (Bioreba AG, Switzerland). RNA was extracted with Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, USA) and tested by RT-PCR using a pair of specific primers to the capsid protein gene (Wang et al., 2008). Expected fragments of 825 bp were obtained and custom-sequenced in both directions (Macrogen, Korea). Nucleotide BLAST analysis of the complete sequence obtained (GenBank accession No. KY308191) showed 97% identity at the nucleotide level with virus isolates from Slovenia, Belarus, Switzerland and Japan (EU796088, FR687356, FR687358, AB948216, respectively). To our knowledge, this is the first report of RBDV infecting raspberry in Argentina. Further research is needed to determine the distribution of this virus in the country and its effects on raspberry plant production.
Instituto de Patología Vegetal
Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Cardozo, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia De Extensión Rural El Bolsón; Argentina
Fil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Cobelo, Claudia Mónica. nstituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia De Extensión Rural El Bolsón; Argentina
Fuente
Journal of Plant Pathology 99 (2) : 539 (2017)
Materia
Raspberries
Dwarfism
Rubus Idaeus
Frambuesa
Virus de las Plantas
Raspberry
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9626
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9626
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling First report of raspberry bushy dwarf virus infecting raspberry in ArgentinaDal Zotto, AngelicaCardozo, AndreaCabrera Mederos, DarielNome Docampo, ClaudiaGiolitti, FabianCobelo, Claudia MonicaRaspberriesDwarfismRubus IdaeusFrambuesaVirus de las PlantasRaspberryIn 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in commercial fields in parallel 42° in the Patagonia region of Argentina. Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA (Ellis et al., 2005; Martin et al., 2013). A survey was conducted to determine if RBDV was also present in Argentinian raspberry fields. Transmission electron microscope observations of leaf dip preparations revealed the presence of spherical particles of 30 nm, resembling those of virus members of the genus Ideaovirus. RBDV was detected in 88 of the 130 plants (68%) tested using double-antibody sandwich ELISA with a specific polyclonal antiserum (Bioreba AG, Switzerland). RNA was extracted with Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, USA) and tested by RT-PCR using a pair of specific primers to the capsid protein gene (Wang et al., 2008). Expected fragments of 825 bp were obtained and custom-sequenced in both directions (Macrogen, Korea). Nucleotide BLAST analysis of the complete sequence obtained (GenBank accession No. KY308191) showed 97% identity at the nucleotide level with virus isolates from Slovenia, Belarus, Switzerland and Japan (EU796088, FR687356, FR687358, AB948216, respectively). To our knowledge, this is the first report of RBDV infecting raspberry in Argentina. Further research is needed to determine the distribution of this virus in the country and its effects on raspberry plant production.Instituto de Patología VegetalFil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Cardozo, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia De Extensión Rural El Bolsón; ArgentinaFil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Cobelo, Claudia Mónica. nstituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia De Extensión Rural El Bolsón; ArgentinaDipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari2021-06-18T18:22:43Z2021-06-18T18:22:43Z2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9626http://www.sipav.org/main/jpp/index.php/jpp/article/view/38961125-46532239-7264 (online)http://dx.doi.org/10.4454/jpp.v99i2.3896Journal of Plant Pathology 99 (2) : 539 (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNFRU-1105073/AR./Generación y desarrollo de tecnología para la detección, seguimiento, predicción, prevención y control de vectores, plagas emergentes y/o limitantes de la producción frutícola argentina.info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.Argentina .......... (nation) (World, South America)info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:15Zoai:localhost:20.500.12123/9626instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:15.805INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First report of raspberry bushy dwarf virus infecting raspberry in Argentina
title First report of raspberry bushy dwarf virus infecting raspberry in Argentina
spellingShingle First report of raspberry bushy dwarf virus infecting raspberry in Argentina
Dal Zotto, Angelica
Raspberries
Dwarfism
Rubus Idaeus
Frambuesa
Virus de las Plantas
Raspberry
title_short First report of raspberry bushy dwarf virus infecting raspberry in Argentina
title_full First report of raspberry bushy dwarf virus infecting raspberry in Argentina
title_fullStr First report of raspberry bushy dwarf virus infecting raspberry in Argentina
title_full_unstemmed First report of raspberry bushy dwarf virus infecting raspberry in Argentina
title_sort First report of raspberry bushy dwarf virus infecting raspberry in Argentina
dc.creator.none.fl_str_mv Dal Zotto, Angelica
Cardozo, Andrea
Cabrera Mederos, Dariel
Nome Docampo, Claudia
Giolitti, Fabian
Cobelo, Claudia Monica
author Dal Zotto, Angelica
author_facet Dal Zotto, Angelica
Cardozo, Andrea
Cabrera Mederos, Dariel
Nome Docampo, Claudia
Giolitti, Fabian
Cobelo, Claudia Monica
author_role author
author2 Cardozo, Andrea
Cabrera Mederos, Dariel
Nome Docampo, Claudia
Giolitti, Fabian
Cobelo, Claudia Monica
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Raspberries
Dwarfism
Rubus Idaeus
Frambuesa
Virus de las Plantas
Raspberry
topic Raspberries
Dwarfism
Rubus Idaeus
Frambuesa
Virus de las Plantas
Raspberry
dc.description.none.fl_txt_mv In 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in commercial fields in parallel 42° in the Patagonia region of Argentina. Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA (Ellis et al., 2005; Martin et al., 2013). A survey was conducted to determine if RBDV was also present in Argentinian raspberry fields. Transmission electron microscope observations of leaf dip preparations revealed the presence of spherical particles of 30 nm, resembling those of virus members of the genus Ideaovirus. RBDV was detected in 88 of the 130 plants (68%) tested using double-antibody sandwich ELISA with a specific polyclonal antiserum (Bioreba AG, Switzerland). RNA was extracted with Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, USA) and tested by RT-PCR using a pair of specific primers to the capsid protein gene (Wang et al., 2008). Expected fragments of 825 bp were obtained and custom-sequenced in both directions (Macrogen, Korea). Nucleotide BLAST analysis of the complete sequence obtained (GenBank accession No. KY308191) showed 97% identity at the nucleotide level with virus isolates from Slovenia, Belarus, Switzerland and Japan (EU796088, FR687356, FR687358, AB948216, respectively). To our knowledge, this is the first report of RBDV infecting raspberry in Argentina. Further research is needed to determine the distribution of this virus in the country and its effects on raspberry plant production.
Instituto de Patología Vegetal
Fil: Dal Zotto, Angelica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Cardozo, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia De Extensión Rural El Bolsón; Argentina
Fil: Cabrera Mederos, Dariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Nome Docampo, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Giolitti, Fabian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Cobelo, Claudia Mónica. nstituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Agencia De Extensión Rural El Bolsón; Argentina
description In 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in commercial fields in parallel 42° in the Patagonia region of Argentina. Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA (Ellis et al., 2005; Martin et al., 2013). A survey was conducted to determine if RBDV was also present in Argentinian raspberry fields. Transmission electron microscope observations of leaf dip preparations revealed the presence of spherical particles of 30 nm, resembling those of virus members of the genus Ideaovirus. RBDV was detected in 88 of the 130 plants (68%) tested using double-antibody sandwich ELISA with a specific polyclonal antiserum (Bioreba AG, Switzerland). RNA was extracted with Spectrum™ Plant Total RNA Kit (Sigma-Aldrich, USA) and tested by RT-PCR using a pair of specific primers to the capsid protein gene (Wang et al., 2008). Expected fragments of 825 bp were obtained and custom-sequenced in both directions (Macrogen, Korea). Nucleotide BLAST analysis of the complete sequence obtained (GenBank accession No. KY308191) showed 97% identity at the nucleotide level with virus isolates from Slovenia, Belarus, Switzerland and Japan (EU796088, FR687356, FR687358, AB948216, respectively). To our knowledge, this is the first report of RBDV infecting raspberry in Argentina. Further research is needed to determine the distribution of this virus in the country and its effects on raspberry plant production.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
2021-06-18T18:22:43Z
2021-06-18T18:22:43Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9626
http://www.sipav.org/main/jpp/index.php/jpp/article/view/3896
1125-4653
2239-7264 (online)
http://dx.doi.org/10.4454/jpp.v99i2.3896
url http://hdl.handle.net/20.500.12123/9626
http://www.sipav.org/main/jpp/index.php/jpp/article/view/3896
http://dx.doi.org/10.4454/jpp.v99i2.3896
identifier_str_mv 1125-4653
2239-7264 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNFRU-1105073/AR./Generación y desarrollo de tecnología para la detección, seguimiento, predicción, prevención y control de vectores, plagas emergentes y/o limitantes de la producción frutícola argentina.
info:eu-repograntAgreement/INTA/PNPV-1135022/AR./Identificación y desarrollo de protocolos para la detección de patógenos de importancia agrícola.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina .......... (nation) (World, South America)
dc.publisher.none.fl_str_mv Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari
publisher.none.fl_str_mv Dipartimento di Scienze del Suolo, della Pianta e degli Alimenti, Università di Bari
dc.source.none.fl_str_mv Journal of Plant Pathology 99 (2) : 539 (2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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