First report of raspberry bushy dwarf virus infecting raspberry in Argentina

Autores
Dal Zotto, A.; Cardozo, A.; Cabrera Mederos, Dariel; Nome, Claudia; Giolitti, Fabián José; Cobelo, Claudia Mónica
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in commercial fields in parallel 42° in the Patagonia region of Argentina. Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA (Ellis et al., 2005; Martin et al., 2013). A survey was conducted to determine if RBDV was also present in Argentinian raspberry fields. Transmission electron microscope observations of leaf dip preparations revealed the presence of spherical particles of 30 nm, resembling those of virus members of the genus Ideaovirus. RBDV was detected in 88 of the 130 plants (68%) tested using double-antibody sandwich ELISA with a specific polyclonal antiserum (Bioreba AG, Switzerland). RNA was extracted with SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA) and tested by RT-PCR using a pair of specific primers to the capsid protein gene (Wang et al., 2008). Expected fragments of 825 bp were obtained and custom-sequenced in both directions (Macrogen, Korea). Nucleotide BLAST analysis of the complete sequence obtained (GenBank accession No. KY308191) showed 97% identity at the nucleotide level with virus isolates from Slovenia, Belarus, Switzerland and Japan (EU796088, FR687356, FR687358, AB948216, respectively). To our knowledge, this is the first report of RBDV infecting raspberry in Argentina. Further research is needed to determine the distribution of this virus in the country and its effects on raspberry plant production.
Fil: Dal Zotto, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Cardozo, A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Agencia de Extensión Rural El Bolsón; Argentina
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nome, Claudia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Cobelo, Claudia Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Agencia de Extensión Rural El Bolsón; Argentina
Materia
RASPBERRY BUSHY DWARF VIRUS
ARGENTINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72753
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/72753
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling First report of raspberry bushy dwarf virus infecting raspberry in ArgentinaDal Zotto, A.Cardozo, A.Cabrera Mederos, DarielNome, ClaudiaGiolitti, Fabián JoséCobelo, Claudia MónicaRASPBERRY BUSHY DWARF VIRUSARGENTINAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in commercial fields in parallel 42° in the Patagonia region of Argentina. Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA (Ellis et al., 2005; Martin et al., 2013). A survey was conducted to determine if RBDV was also present in Argentinian raspberry fields. Transmission electron microscope observations of leaf dip preparations revealed the presence of spherical particles of 30 nm, resembling those of virus members of the genus Ideaovirus. RBDV was detected in 88 of the 130 plants (68%) tested using double-antibody sandwich ELISA with a specific polyclonal antiserum (Bioreba AG, Switzerland). RNA was extracted with SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA) and tested by RT-PCR using a pair of specific primers to the capsid protein gene (Wang et al., 2008). Expected fragments of 825 bp were obtained and custom-sequenced in both directions (Macrogen, Korea). Nucleotide BLAST analysis of the complete sequence obtained (GenBank accession No. KY308191) showed 97% identity at the nucleotide level with virus isolates from Slovenia, Belarus, Switzerland and Japan (EU796088, FR687356, FR687358, AB948216, respectively). To our knowledge, this is the first report of RBDV infecting raspberry in Argentina. Further research is needed to determine the distribution of this virus in the country and its effects on raspberry plant production.Fil: Dal Zotto, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Cardozo, A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Agencia de Extensión Rural El Bolsón; ArgentinaFil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nome, Claudia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Cobelo, Claudia Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Agencia de Extensión Rural El Bolsón; ArgentinaEdizioni Ets2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72753Dal Zotto, A.; Cardozo, A.; Cabrera Mederos, Dariel; Nome, Claudia; Giolitti, Fabián José; et al.; First report of raspberry bushy dwarf virus infecting raspberry in Argentina; Edizioni Ets; Journal Of Plant Pathology; 99; 2; 11-20171125-4653CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sipav.org/main/jpp/index.php/jpp/article/view/3896info:eu-repo/semantics/altIdentifier/doi/10.4454/jpp.v99i2.3896info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:49:23Zoai:ri.conicet.gov.ar:11336/72753instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:49:24.035CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv First report of raspberry bushy dwarf virus infecting raspberry in Argentina
title First report of raspberry bushy dwarf virus infecting raspberry in Argentina
spellingShingle First report of raspberry bushy dwarf virus infecting raspberry in Argentina
Dal Zotto, A.
RASPBERRY BUSHY DWARF VIRUS
ARGENTINA
title_short First report of raspberry bushy dwarf virus infecting raspberry in Argentina
title_full First report of raspberry bushy dwarf virus infecting raspberry in Argentina
title_fullStr First report of raspberry bushy dwarf virus infecting raspberry in Argentina
title_full_unstemmed First report of raspberry bushy dwarf virus infecting raspberry in Argentina
title_sort First report of raspberry bushy dwarf virus infecting raspberry in Argentina
dc.creator.none.fl_str_mv Dal Zotto, A.
Cardozo, A.
Cabrera Mederos, Dariel
Nome, Claudia
Giolitti, Fabián José
Cobelo, Claudia Mónica
author Dal Zotto, A.
author_facet Dal Zotto, A.
Cardozo, A.
Cabrera Mederos, Dariel
Nome, Claudia
Giolitti, Fabián José
Cobelo, Claudia Mónica
author_role author
author2 Cardozo, A.
Cabrera Mederos, Dariel
Nome, Claudia
Giolitti, Fabián José
Cobelo, Claudia Mónica
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv RASPBERRY BUSHY DWARF VIRUS
ARGENTINA
topic RASPBERRY BUSHY DWARF VIRUS
ARGENTINA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in commercial fields in parallel 42° in the Patagonia region of Argentina. Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA (Ellis et al., 2005; Martin et al., 2013). A survey was conducted to determine if RBDV was also present in Argentinian raspberry fields. Transmission electron microscope observations of leaf dip preparations revealed the presence of spherical particles of 30 nm, resembling those of virus members of the genus Ideaovirus. RBDV was detected in 88 of the 130 plants (68%) tested using double-antibody sandwich ELISA with a specific polyclonal antiserum (Bioreba AG, Switzerland). RNA was extracted with SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA) and tested by RT-PCR using a pair of specific primers to the capsid protein gene (Wang et al., 2008). Expected fragments of 825 bp were obtained and custom-sequenced in both directions (Macrogen, Korea). Nucleotide BLAST analysis of the complete sequence obtained (GenBank accession No. KY308191) showed 97% identity at the nucleotide level with virus isolates from Slovenia, Belarus, Switzerland and Japan (EU796088, FR687356, FR687358, AB948216, respectively). To our knowledge, this is the first report of RBDV infecting raspberry in Argentina. Further research is needed to determine the distribution of this virus in the country and its effects on raspberry plant production.
Fil: Dal Zotto, A.. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Cardozo, A.. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Agencia de Extensión Rural El Bolsón; Argentina
Fil: Cabrera Mederos, Dariel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nome, Claudia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Giolitti, Fabián José. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Cobelo, Claudia Mónica. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Patagonia Norte. Estación Experimental Agropecuaria San Carlos de Bariloche. Agencia de Extensión Rural El Bolsón; Argentina
description In 2014-2015, raspberry (Rubus idaeus) cv. Autum bliss plants with dwarfism and foliar chlorosis were observed in commercial fields in parallel 42° in the Patagonia region of Argentina. Symptoms were similar to those observed in raspberry infected by Raspberry bushy dwarf virus (RBDV) in the USA (Ellis et al., 2005; Martin et al., 2013). A survey was conducted to determine if RBDV was also present in Argentinian raspberry fields. Transmission electron microscope observations of leaf dip preparations revealed the presence of spherical particles of 30 nm, resembling those of virus members of the genus Ideaovirus. RBDV was detected in 88 of the 130 plants (68%) tested using double-antibody sandwich ELISA with a specific polyclonal antiserum (Bioreba AG, Switzerland). RNA was extracted with SpectrumTM Plant Total RNA Kit (Sigma-Aldrich, USA) and tested by RT-PCR using a pair of specific primers to the capsid protein gene (Wang et al., 2008). Expected fragments of 825 bp were obtained and custom-sequenced in both directions (Macrogen, Korea). Nucleotide BLAST analysis of the complete sequence obtained (GenBank accession No. KY308191) showed 97% identity at the nucleotide level with virus isolates from Slovenia, Belarus, Switzerland and Japan (EU796088, FR687356, FR687358, AB948216, respectively). To our knowledge, this is the first report of RBDV infecting raspberry in Argentina. Further research is needed to determine the distribution of this virus in the country and its effects on raspberry plant production.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72753
Dal Zotto, A.; Cardozo, A.; Cabrera Mederos, Dariel; Nome, Claudia; Giolitti, Fabián José; et al.; First report of raspberry bushy dwarf virus infecting raspberry in Argentina; Edizioni Ets; Journal Of Plant Pathology; 99; 2; 11-2017
1125-4653
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72753
identifier_str_mv Dal Zotto, A.; Cardozo, A.; Cabrera Mederos, Dariel; Nome, Claudia; Giolitti, Fabián José; et al.; First report of raspberry bushy dwarf virus infecting raspberry in Argentina; Edizioni Ets; Journal Of Plant Pathology; 99; 2; 11-2017
1125-4653
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sipav.org/main/jpp/index.php/jpp/article/view/3896
info:eu-repo/semantics/altIdentifier/doi/10.4454/jpp.v99i2.3896
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Edizioni Ets
publisher.none.fl_str_mv Edizioni Ets
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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