Co-expression of bovine leukemia virus and bovine foamy virus-derived miRNAs in naturally infected cattle

Autores
Petersen, Marcos Iván; Suarez Archilla, Guillermo; Perez, C.V.; Gonzalez, Diego D.; Trono, Karina Gabriela; Mongini, Claudia; Jaworski, Juan Pablo; Carignano, Hugo Adrian
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Among the viruses that encode microRNAs (miRNAs) and infect cattle, such as bovine leukemia virus (BLV), bovine foamy virus (BFV), and Bovine herpesviruses, BLV has gained attention due to the critical role that BLV-miRNAs play in inducing lymphosarcoma in infected animals. BLV is highly prevalent in the Americas and negatively affects dairy herds, primarily due to restrictions on the commercialization of dairy products from infected animals and a decrease in milk production. Co-infections involving BLV and BFV appear to be common in cattle. Considering the ability of foamy viruses to cross species barriers, preventing their presence within the food chain is essential. We identified the co-expression of seven BLV-derived miRNAs (blv-miR-B1-3p, blv-miR-B2-5p, blv-miR-B2-3p, blv-miR-B3-5p, blv-miR-B3-3p, blv-miR-B4-3p, and blv-miR-B5-5p) and three BFV-derived miRNAs (bfv-miR-BF1-5p, bfv-miR-BF1-3p, and bfv-miR-BF2-5p) in naturally BLV-infected cows. Besides, seven differentially expressed bovine miRNAs (bta-miR-375, bta-miR-133a, bta-miR-677, bta-miR-1, bta-miR-3613a, bta-miR-9-5p, and bta-miR-95) were identified between cows with high BLV proviral load and uninfected counterparts (|fold change| > 1.5 and q-value < 0.05). A comprehensive analysis of protein-protein interaction networks for genes targeted by viral and host-derived miRNAs suggests a functional convergence on key pathways related to immune response, tumorigenesis, and chromatin remodeling. Although BLV- and BFV-derived miRNAs target different genes, these targets participate in shared biological processes. This convergence might reflect a coordinated viral influence on host functions, with implications for disease progression and the increased dissemination of BFV. These findings offer new perspectives for creating diagnostic and treatment approaches to manage viral persistence and tumorigenesis in cattle.
Instituto de Virología
Fil: Petersen, Marcos Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Petersen, Marcos Iván. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Perez, C.V. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Perez, C.V. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Diego D..Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Gonzalez, Diego D. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mongini, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Mongini, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jaworski, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Jaworski, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Carignano, Hugo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Microbiology Spectrum 13 (10) : 1-19 (October 2025)
Materia
Ganado Bovino
Enfermedades de los Animales
Virus Leucemia Bovina
MicroARN
Diagnóstico
Cattle
Animal Diseases
Bovine Leukemia Virus
MicroRNA
Diagnosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24768
Acceder
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oai_identifier_str oai:localhost:20.500.12123/24768
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Co-expression of bovine leukemia virus and bovine foamy virus-derived miRNAs in naturally infected cattlePetersen, Marcos IvánSuarez Archilla, GuillermoPerez, C.V.Gonzalez, Diego D.Trono, Karina GabrielaMongini, ClaudiaJaworski, Juan PabloCarignano, Hugo AdrianGanado BovinoEnfermedades de los AnimalesVirus Leucemia BovinaMicroARNDiagnósticoCattleAnimal DiseasesBovine Leukemia VirusMicroRNADiagnosisAmong the viruses that encode microRNAs (miRNAs) and infect cattle, such as bovine leukemia virus (BLV), bovine foamy virus (BFV), and Bovine herpesviruses, BLV has gained attention due to the critical role that BLV-miRNAs play in inducing lymphosarcoma in infected animals. BLV is highly prevalent in the Americas and negatively affects dairy herds, primarily due to restrictions on the commercialization of dairy products from infected animals and a decrease in milk production. Co-infections involving BLV and BFV appear to be common in cattle. Considering the ability of foamy viruses to cross species barriers, preventing their presence within the food chain is essential. We identified the co-expression of seven BLV-derived miRNAs (blv-miR-B1-3p, blv-miR-B2-5p, blv-miR-B2-3p, blv-miR-B3-5p, blv-miR-B3-3p, blv-miR-B4-3p, and blv-miR-B5-5p) and three BFV-derived miRNAs (bfv-miR-BF1-5p, bfv-miR-BF1-3p, and bfv-miR-BF2-5p) in naturally BLV-infected cows. Besides, seven differentially expressed bovine miRNAs (bta-miR-375, bta-miR-133a, bta-miR-677, bta-miR-1, bta-miR-3613a, bta-miR-9-5p, and bta-miR-95) were identified between cows with high BLV proviral load and uninfected counterparts (|fold change| > 1.5 and q-value < 0.05). A comprehensive analysis of protein-protein interaction networks for genes targeted by viral and host-derived miRNAs suggests a functional convergence on key pathways related to immune response, tumorigenesis, and chromatin remodeling. Although BLV- and BFV-derived miRNAs target different genes, these targets participate in shared biological processes. This convergence might reflect a coordinated viral influence on host functions, with implications for disease progression and the increased dissemination of BFV. These findings offer new perspectives for creating diagnostic and treatment approaches to manage viral persistence and tumorigenesis in cattle.Instituto de VirologíaFil: Petersen, Marcos Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Petersen, Marcos Iván. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Perez, C.V. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Perez, C.V. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gonzalez, Diego D..Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Gonzalez, Diego D. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mongini, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Mongini, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jaworski, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Jaworski, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Carignano, Hugo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2025-12-26T13:59:53Z2025-12-26T13:59:53Z2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24768https://journals.asm.org/doi/10.1128/spectrum.01755-252165-0497https://doi.org/10.1128/spectrum.01755-25Microbiology Spectrum 13 (10) : 1-19 (October 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Saludinfo:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Saludinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-01-08T10:41:01Zoai:localhost:20.500.12123/24768instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-01-08 10:41:01.522INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Co-expression of bovine leukemia virus and bovine foamy virus-derived miRNAs in naturally infected cattle
title Co-expression of bovine leukemia virus and bovine foamy virus-derived miRNAs in naturally infected cattle
spellingShingle Co-expression of bovine leukemia virus and bovine foamy virus-derived miRNAs in naturally infected cattle
Petersen, Marcos Iván
Ganado Bovino
Enfermedades de los Animales
Virus Leucemia Bovina
MicroARN
Diagnóstico
Cattle
Animal Diseases
Bovine Leukemia Virus
MicroRNA
Diagnosis
title_short Co-expression of bovine leukemia virus and bovine foamy virus-derived miRNAs in naturally infected cattle
title_full Co-expression of bovine leukemia virus and bovine foamy virus-derived miRNAs in naturally infected cattle
title_fullStr Co-expression of bovine leukemia virus and bovine foamy virus-derived miRNAs in naturally infected cattle
title_full_unstemmed Co-expression of bovine leukemia virus and bovine foamy virus-derived miRNAs in naturally infected cattle
title_sort Co-expression of bovine leukemia virus and bovine foamy virus-derived miRNAs in naturally infected cattle
dc.creator.none.fl_str_mv Petersen, Marcos Iván
Suarez Archilla, Guillermo
Perez, C.V.
Gonzalez, Diego D.
Trono, Karina Gabriela
Mongini, Claudia
Jaworski, Juan Pablo
Carignano, Hugo Adrian
author Petersen, Marcos Iván
author_facet Petersen, Marcos Iván
Suarez Archilla, Guillermo
Perez, C.V.
Gonzalez, Diego D.
Trono, Karina Gabriela
Mongini, Claudia
Jaworski, Juan Pablo
Carignano, Hugo Adrian
author_role author
author2 Suarez Archilla, Guillermo
Perez, C.V.
Gonzalez, Diego D.
Trono, Karina Gabriela
Mongini, Claudia
Jaworski, Juan Pablo
Carignano, Hugo Adrian
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Virus Leucemia Bovina
MicroARN
Diagnóstico
Cattle
Animal Diseases
Bovine Leukemia Virus
MicroRNA
Diagnosis
topic Ganado Bovino
Enfermedades de los Animales
Virus Leucemia Bovina
MicroARN
Diagnóstico
Cattle
Animal Diseases
Bovine Leukemia Virus
MicroRNA
Diagnosis
dc.description.none.fl_txt_mv Among the viruses that encode microRNAs (miRNAs) and infect cattle, such as bovine leukemia virus (BLV), bovine foamy virus (BFV), and Bovine herpesviruses, BLV has gained attention due to the critical role that BLV-miRNAs play in inducing lymphosarcoma in infected animals. BLV is highly prevalent in the Americas and negatively affects dairy herds, primarily due to restrictions on the commercialization of dairy products from infected animals and a decrease in milk production. Co-infections involving BLV and BFV appear to be common in cattle. Considering the ability of foamy viruses to cross species barriers, preventing their presence within the food chain is essential. We identified the co-expression of seven BLV-derived miRNAs (blv-miR-B1-3p, blv-miR-B2-5p, blv-miR-B2-3p, blv-miR-B3-5p, blv-miR-B3-3p, blv-miR-B4-3p, and blv-miR-B5-5p) and three BFV-derived miRNAs (bfv-miR-BF1-5p, bfv-miR-BF1-3p, and bfv-miR-BF2-5p) in naturally BLV-infected cows. Besides, seven differentially expressed bovine miRNAs (bta-miR-375, bta-miR-133a, bta-miR-677, bta-miR-1, bta-miR-3613a, bta-miR-9-5p, and bta-miR-95) were identified between cows with high BLV proviral load and uninfected counterparts (|fold change| > 1.5 and q-value < 0.05). A comprehensive analysis of protein-protein interaction networks for genes targeted by viral and host-derived miRNAs suggests a functional convergence on key pathways related to immune response, tumorigenesis, and chromatin remodeling. Although BLV- and BFV-derived miRNAs target different genes, these targets participate in shared biological processes. This convergence might reflect a coordinated viral influence on host functions, with implications for disease progression and the increased dissemination of BFV. These findings offer new perspectives for creating diagnostic and treatment approaches to manage viral persistence and tumorigenesis in cattle.
Instituto de Virología
Fil: Petersen, Marcos Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Petersen, Marcos Iván. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Suarez Archilla, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Perez, C.V. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Perez, C.V. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gonzalez, Diego D..Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Gonzalez, Diego D. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mongini, Claudia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Mongini, Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jaworski, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Jaworski, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Carignano, Hugo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Among the viruses that encode microRNAs (miRNAs) and infect cattle, such as bovine leukemia virus (BLV), bovine foamy virus (BFV), and Bovine herpesviruses, BLV has gained attention due to the critical role that BLV-miRNAs play in inducing lymphosarcoma in infected animals. BLV is highly prevalent in the Americas and negatively affects dairy herds, primarily due to restrictions on the commercialization of dairy products from infected animals and a decrease in milk production. Co-infections involving BLV and BFV appear to be common in cattle. Considering the ability of foamy viruses to cross species barriers, preventing their presence within the food chain is essential. We identified the co-expression of seven BLV-derived miRNAs (blv-miR-B1-3p, blv-miR-B2-5p, blv-miR-B2-3p, blv-miR-B3-5p, blv-miR-B3-3p, blv-miR-B4-3p, and blv-miR-B5-5p) and three BFV-derived miRNAs (bfv-miR-BF1-5p, bfv-miR-BF1-3p, and bfv-miR-BF2-5p) in naturally BLV-infected cows. Besides, seven differentially expressed bovine miRNAs (bta-miR-375, bta-miR-133a, bta-miR-677, bta-miR-1, bta-miR-3613a, bta-miR-9-5p, and bta-miR-95) were identified between cows with high BLV proviral load and uninfected counterparts (|fold change| > 1.5 and q-value < 0.05). A comprehensive analysis of protein-protein interaction networks for genes targeted by viral and host-derived miRNAs suggests a functional convergence on key pathways related to immune response, tumorigenesis, and chromatin remodeling. Although BLV- and BFV-derived miRNAs target different genes, these targets participate in shared biological processes. This convergence might reflect a coordinated viral influence on host functions, with implications for disease progression and the increased dissemination of BFV. These findings offer new perspectives for creating diagnostic and treatment approaches to manage viral persistence and tumorigenesis in cattle.
publishDate 2025
dc.date.none.fl_str_mv 2025-12-26T13:59:53Z
2025-12-26T13:59:53Z
2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24768
https://journals.asm.org/doi/10.1128/spectrum.01755-25
2165-0497
https://doi.org/10.1128/spectrum.01755-25
url http://hdl.handle.net/20.500.12123/24768
https://journals.asm.org/doi/10.1128/spectrum.01755-25
https://doi.org/10.1128/spectrum.01755-25
identifier_str_mv 2165-0497
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud
info:eu-repograntAgreement/INTA/2023-PD-L06-I114, Desarrollo y aplicación de métodos diagnósticos y epidemiológicos para la producción pecuaria sustentable y agroalimentaria en humanos con foco en Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Microbiology Spectrum 13 (10) : 1-19 (October 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
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instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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