Absence of detectable bovine leukemia virus miRNAs in human cancer small RNA-seq datasets

Autores
Jaworski, Juan Pablo
Año de publicación
2026
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine leukemia virus (BLV) encodes 10 mature microRNAs (miRNAs) that are highly expressed in infected cattle and have been implicated in oncogenic mechanisms. A potential association between BLV and human cancer remains controversial: while several reports have detected BLV proviral DNA in human breast tissue, high-throughput sequencing analyses have failed to identify BLV nucleic acids in human tumors. Here, we performed a high-sensitivity screen for BLV miRNAs across 335 publicly available human small RNA-seq data sets, including breast cancer (BCA), acute lymphoblastic leukemia, acute myeloid leukemia, chronic lymphocytic leukemia, and healthy controls, totaling approximately 1.98 billion trimmed reads. To validate our analytical workflow, six small RNA data sets from BLV-infected and uninfected cattle were processed in parallel. Across the human data sets, only 60 reads (0.000003%) aligned to BLV miRNA reference sequences, predominantly STAR strands or sequences containing mismatches—patterns consistent with background noise rather than biologically meaningful viral miRNAs. In contrast, BLV miRNAs were robustly detected across BLV-infected cattle samples, confirming pipeline sensitivity for canonical BLV miRNAs. These results demonstrate that BLV miRNAs are absent or below the operational detection threshold in human BCA and human leukemia miRNomes using standard small-RNA sequencing workflows and canonical reference sequences. IMPORTANCE
Instituto de Virología
Fil: Jaworski, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Jaworski, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Microbiology Spectrum 14 (4) : e03818-25 (April 2026)
Materia
RNA
Humans
Bovine Leukemia Virus
ARN
Género Humano
Virus Leucemia Bovina
Micro-RNA
Micro-ARN
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/26145
Acceder
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oai_identifier_str oai:localhost:20.500.12123/26145
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Absence of detectable bovine leukemia virus miRNAs in human cancer small RNA-seq datasetsJaworski, Juan PabloRNAHumansBovine Leukemia VirusARNGénero HumanoVirus Leucemia BovinaMicro-RNAMicro-ARNBovine leukemia virus (BLV) encodes 10 mature microRNAs (miRNAs) that are highly expressed in infected cattle and have been implicated in oncogenic mechanisms. A potential association between BLV and human cancer remains controversial: while several reports have detected BLV proviral DNA in human breast tissue, high-throughput sequencing analyses have failed to identify BLV nucleic acids in human tumors. Here, we performed a high-sensitivity screen for BLV miRNAs across 335 publicly available human small RNA-seq data sets, including breast cancer (BCA), acute lymphoblastic leukemia, acute myeloid leukemia, chronic lymphocytic leukemia, and healthy controls, totaling approximately 1.98 billion trimmed reads. To validate our analytical workflow, six small RNA data sets from BLV-infected and uninfected cattle were processed in parallel. Across the human data sets, only 60 reads (0.000003%) aligned to BLV miRNA reference sequences, predominantly STAR strands or sequences containing mismatches—patterns consistent with background noise rather than biologically meaningful viral miRNAs. In contrast, BLV miRNAs were robustly detected across BLV-infected cattle samples, confirming pipeline sensitivity for canonical BLV miRNAs. These results demonstrate that BLV miRNAs are absent or below the operational detection threshold in human BCA and human leukemia miRNomes using standard small-RNA sequencing workflows and canonical reference sequences. IMPORTANCEInstituto de VirologíaFil: Jaworski, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Jaworski, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAmerican Society for Microbiology2026-05-12T09:52:32Z2026-05-12T09:52:32Z2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/26145https://journals.asm.org/doi/10.1128/spectrum.03818-252165-0497https://doi.org/10.1128/spectrum.03818-25Microbiology Spectrum 14 (4) : e03818-25 (April 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-05-14T10:43:35Zoai:localhost:20.500.12123/26145instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-05-14 10:43:35.755INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Absence of detectable bovine leukemia virus miRNAs in human cancer small RNA-seq datasets
title Absence of detectable bovine leukemia virus miRNAs in human cancer small RNA-seq datasets
spellingShingle Absence of detectable bovine leukemia virus miRNAs in human cancer small RNA-seq datasets
Jaworski, Juan Pablo
RNA
Humans
Bovine Leukemia Virus
ARN
Género Humano
Virus Leucemia Bovina
Micro-RNA
Micro-ARN
title_short Absence of detectable bovine leukemia virus miRNAs in human cancer small RNA-seq datasets
title_full Absence of detectable bovine leukemia virus miRNAs in human cancer small RNA-seq datasets
title_fullStr Absence of detectable bovine leukemia virus miRNAs in human cancer small RNA-seq datasets
title_full_unstemmed Absence of detectable bovine leukemia virus miRNAs in human cancer small RNA-seq datasets
title_sort Absence of detectable bovine leukemia virus miRNAs in human cancer small RNA-seq datasets
dc.creator.none.fl_str_mv Jaworski, Juan Pablo
author Jaworski, Juan Pablo
author_facet Jaworski, Juan Pablo
author_role author
dc.subject.none.fl_str_mv RNA
Humans
Bovine Leukemia Virus
ARN
Género Humano
Virus Leucemia Bovina
Micro-RNA
Micro-ARN
topic RNA
Humans
Bovine Leukemia Virus
ARN
Género Humano
Virus Leucemia Bovina
Micro-RNA
Micro-ARN
dc.description.none.fl_txt_mv Bovine leukemia virus (BLV) encodes 10 mature microRNAs (miRNAs) that are highly expressed in infected cattle and have been implicated in oncogenic mechanisms. A potential association between BLV and human cancer remains controversial: while several reports have detected BLV proviral DNA in human breast tissue, high-throughput sequencing analyses have failed to identify BLV nucleic acids in human tumors. Here, we performed a high-sensitivity screen for BLV miRNAs across 335 publicly available human small RNA-seq data sets, including breast cancer (BCA), acute lymphoblastic leukemia, acute myeloid leukemia, chronic lymphocytic leukemia, and healthy controls, totaling approximately 1.98 billion trimmed reads. To validate our analytical workflow, six small RNA data sets from BLV-infected and uninfected cattle were processed in parallel. Across the human data sets, only 60 reads (0.000003%) aligned to BLV miRNA reference sequences, predominantly STAR strands or sequences containing mismatches—patterns consistent with background noise rather than biologically meaningful viral miRNAs. In contrast, BLV miRNAs were robustly detected across BLV-infected cattle samples, confirming pipeline sensitivity for canonical BLV miRNAs. These results demonstrate that BLV miRNAs are absent or below the operational detection threshold in human BCA and human leukemia miRNomes using standard small-RNA sequencing workflows and canonical reference sequences. IMPORTANCE
Instituto de Virología
Fil: Jaworski, Juan Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Jaworski, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Bovine leukemia virus (BLV) encodes 10 mature microRNAs (miRNAs) that are highly expressed in infected cattle and have been implicated in oncogenic mechanisms. A potential association between BLV and human cancer remains controversial: while several reports have detected BLV proviral DNA in human breast tissue, high-throughput sequencing analyses have failed to identify BLV nucleic acids in human tumors. Here, we performed a high-sensitivity screen for BLV miRNAs across 335 publicly available human small RNA-seq data sets, including breast cancer (BCA), acute lymphoblastic leukemia, acute myeloid leukemia, chronic lymphocytic leukemia, and healthy controls, totaling approximately 1.98 billion trimmed reads. To validate our analytical workflow, six small RNA data sets from BLV-infected and uninfected cattle were processed in parallel. Across the human data sets, only 60 reads (0.000003%) aligned to BLV miRNA reference sequences, predominantly STAR strands or sequences containing mismatches—patterns consistent with background noise rather than biologically meaningful viral miRNAs. In contrast, BLV miRNAs were robustly detected across BLV-infected cattle samples, confirming pipeline sensitivity for canonical BLV miRNAs. These results demonstrate that BLV miRNAs are absent or below the operational detection threshold in human BCA and human leukemia miRNomes using standard small-RNA sequencing workflows and canonical reference sequences. IMPORTANCE
publishDate 2026
dc.date.none.fl_str_mv 2026-05-12T09:52:32Z
2026-05-12T09:52:32Z
2026-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/26145
https://journals.asm.org/doi/10.1128/spectrum.03818-25
2165-0497
https://doi.org/10.1128/spectrum.03818-25
url http://hdl.handle.net/20.500.12123/26145
https://journals.asm.org/doi/10.1128/spectrum.03818-25
https://doi.org/10.1128/spectrum.03818-25
identifier_str_mv 2165-0497
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv Microbiology Spectrum 14 (4) : e03818-25 (April 2026)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.115601

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