Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo

Autores
Gillet, Nicolas A.; Hamaidia, Malik; Brogniez, Alix de; Gutierrez, Gerónimo; Renotte, Nathalie; Reichert, Michal; Trono, Karina Gabriela; Willems, Luc
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Retroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundantly expressed in B-cell tumors in the absence of significant levels of genomic and subgenomic viral RNAs. Using deep RNA sequencing and functional reporter assays, we show that miRNAs mediate the expression of genes involved in cell signaling, cancer and immunity. We further demonstrate that BLV miRNAs are essential to induce B-cell tumors in an experimental model and to promote efficient viral replication in the natural host.
Inst.de Virología
Fil: Gillet, Nicolas A. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica
Fil: Hamaidia, Malik. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica
Fil: Renotte, Nathalie. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica
Fil: Reichert, Michal. National Veterinary Research Institute; Polonia
Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Willems, Luc. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica
Fuente
PLOS Pathogens 12(4) : e1005588 (2016)
Materia
Enfermedades de los Animales
Virus Leucemia Bovina
ARN
Oncogenicidad
Animal Diseases
Bovine Leukaemia Virus
RNA
Oncogenicity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1218
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1218
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network_name_str INTA Digital (INTA)
spelling Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivoGillet, Nicolas A.Hamaidia, MalikBrogniez, Alix deGutierrez, GerónimoRenotte, NathalieReichert, MichalTrono, Karina GabrielaWillems, LucEnfermedades de los AnimalesVirus Leucemia BovinaARNOncogenicidadAnimal DiseasesBovine Leukaemia VirusRNAOncogenicityRetroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundantly expressed in B-cell tumors in the absence of significant levels of genomic and subgenomic viral RNAs. Using deep RNA sequencing and functional reporter assays, we show that miRNAs mediate the expression of genes involved in cell signaling, cancer and immunity. We further demonstrate that BLV miRNAs are essential to induce B-cell tumors in an experimental model and to promote efficient viral replication in the natural host.Inst.de VirologíaFil: Gillet, Nicolas A. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; BélgicaFil: Hamaidia, Malik. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; BélgicaFil: Renotte, Nathalie. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; BélgicaFil: Reichert, Michal. National Veterinary Research Institute; PoloniaFil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Willems, Luc. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica2017-09-14T13:41:45Z2017-09-14T13:41:45Z2016-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1218http://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005588&type=printableGillet NA, Hamaidia M, de Brogniez A, Gutiérrez G, Renotte N, Reichert M, et al. (2016) Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo. PLoS Pathog 12(4): e10055881553-7366 (Print)1553-7374 (Online)https://doi.org/10.1371/journal.ppat.1005588PLOS Pathogens 12(4) : e1005588 (2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:00Zoai:localhost:20.500.12123/1218instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:01.148INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo
title Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo
spellingShingle Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo
Gillet, Nicolas A.
Enfermedades de los Animales
Virus Leucemia Bovina
ARN
Oncogenicidad
Animal Diseases
Bovine Leukaemia Virus
RNA
Oncogenicity
title_short Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo
title_full Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo
title_fullStr Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo
title_full_unstemmed Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo
title_sort Bovine leukemia virus small noncoding RNAs are functional elements that regulate replication and contribute to oncogenesis in vivo
dc.creator.none.fl_str_mv Gillet, Nicolas A.
Hamaidia, Malik
Brogniez, Alix de
Gutierrez, Gerónimo
Renotte, Nathalie
Reichert, Michal
Trono, Karina Gabriela
Willems, Luc
author Gillet, Nicolas A.
author_facet Gillet, Nicolas A.
Hamaidia, Malik
Brogniez, Alix de
Gutierrez, Gerónimo
Renotte, Nathalie
Reichert, Michal
Trono, Karina Gabriela
Willems, Luc
author_role author
author2 Hamaidia, Malik
Brogniez, Alix de
Gutierrez, Gerónimo
Renotte, Nathalie
Reichert, Michal
Trono, Karina Gabriela
Willems, Luc
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Virus Leucemia Bovina
ARN
Oncogenicidad
Animal Diseases
Bovine Leukaemia Virus
RNA
Oncogenicity
topic Enfermedades de los Animales
Virus Leucemia Bovina
ARN
Oncogenicidad
Animal Diseases
Bovine Leukaemia Virus
RNA
Oncogenicity
dc.description.none.fl_txt_mv Retroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundantly expressed in B-cell tumors in the absence of significant levels of genomic and subgenomic viral RNAs. Using deep RNA sequencing and functional reporter assays, we show that miRNAs mediate the expression of genes involved in cell signaling, cancer and immunity. We further demonstrate that BLV miRNAs are essential to induce B-cell tumors in an experimental model and to promote efficient viral replication in the natural host.
Inst.de Virología
Fil: Gillet, Nicolas A. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica
Fil: Hamaidia, Malik. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica
Fil: Renotte, Nathalie. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica
Fil: Reichert, Michal. National Veterinary Research Institute; Polonia
Fil: Gutierrez, Gerónimo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Willems, Luc. University of Liège. Molecular and Cellular Epigenetics, Interdisciplinary Cluster for Applied Genoproteomics; Bélgica. University of Liège. Molecular and Cellular Biology, Gembloux Agro-Bio Tech; Bélgica
description Retroviruses are not expected to encode miRNAs because of the potential problem of self-cleavage of their genomic RNAs. This assumption has recently been challenged by experiments showing that bovine leukemia virus (BLV) encodes miRNAs from intragenomic Pol III promoters. The BLV miRNAs are abundantly expressed in B-cell tumors in the absence of significant levels of genomic and subgenomic viral RNAs. Using deep RNA sequencing and functional reporter assays, we show that miRNAs mediate the expression of genes involved in cell signaling, cancer and immunity. We further demonstrate that BLV miRNAs are essential to induce B-cell tumors in an experimental model and to promote efficient viral replication in the natural host.
publishDate 2016
dc.date.none.fl_str_mv 2016-04
2017-09-14T13:41:45Z
2017-09-14T13:41:45Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1218
http://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005588&type=printable
Gillet NA, Hamaidia M, de Brogniez A, Gutiérrez G, Renotte N, Reichert M, et al. (2016) Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo. PLoS Pathog 12(4): e1005588
1553-7366 (Print)
1553-7374 (Online)
https://doi.org/10.1371/journal.ppat.1005588
url http://hdl.handle.net/20.500.12123/1218
http://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1005588&type=printable
https://doi.org/10.1371/journal.ppat.1005588
identifier_str_mv Gillet NA, Hamaidia M, de Brogniez A, Gutiérrez G, Renotte N, Reichert M, et al. (2016) Bovine Leukemia Virus Small Noncoding RNAs Are Functional Elements That Regulate Replication and Contribute to Oncogenesis In Vivo. PLoS Pathog 12(4): e1005588
1553-7366 (Print)
1553-7374 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv PLOS Pathogens 12(4) : e1005588 (2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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