Kosakonia radicincitans and Cryptococcus laurentii controlled Penicillium expansum rot and decreased patulin production at 4 and 25 °C

Autores
Lambrese, Yesica; Sansone, Gabriela; Sanz Ferramola, Maria Isabel; Di Masi, Susana Noemi; Raba, Julio; Calvente, Viviana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
In the present work, we evaluated the effects of a mixture of biocontrol agents against two toxigenic strains of Penicillium expansum isolated in Argentine Patagonia from pome fruits. The two strains, INTA-5 and INTA-10, were previusly selected among ten strains coming from the Alto Valle (Rio Negro-Argentina) for their high production of patulin. For the biocontrol, Kosakonia radicincitans, Cryptococcus laurentii, and Rhodosporidium fluviale were tested in vitro experiments on Potato Dextrose Agar (PDA) dishes against the INTA-5 and INTA-10 strains. The bacterium K. radicincitans and the yeast C. laurentii were selected to be used in a mixture due to their capacity to control the fungus and reduce the mycotoxin severely. In vitro assays with the mixture showed a high antagonism against P. expansum INTA-5 and INTA-10, at 21 d of incubation at 25 °C and a patulin reduction of 98%. The mixture of microorganisms was also effective in apples stored at 25 °C for 10 d and 4 °C for 30 d. At cold storage, the mixture controlled moderately the development of rot and decreased patulin concentration. At 25 °C, the pathogen's optimal growth temperature, the mixture of Biological Control Agent (BCAs) assured both the control of rot and decrease of patulin concentration. The combination of two microorganisms, with different requirements and abilities, resulted in a mix with a strong antagonism against P. expansum with the capability to decrease the patulin concentration. Treatment with the selected mixture could be a good option for controlling strains with different behaviours and in different environmental conditions.
EEA Alto Valle
Fil: Lambrese, Yesica. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; Argentina
Fil: Lambrese, Yesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina.
Fil: Lambrese, Yesica. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Sansone, Gabriela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; Argentina
Fil: Sanz Ferramola, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Di Masi, Susana Noemi. Instituto Nacional Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Calvente, Viviana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; Argentina
Fuente
Food Microbiology 100 : 103863 (December 2021)
Materia
Penicillium expansum
Podredumbres
Control Biológico
Patulina
Tecnología Postcosecha
Rots
Biological Control
Patulin
Postharvest Technology
Kosakonia radicincitans
Cryptococcus laurentii
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10768
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10768
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Kosakonia radicincitans and Cryptococcus laurentii controlled Penicillium expansum rot and decreased patulin production at 4 and 25 °CLambrese, YesicaSansone, GabrielaSanz Ferramola, Maria IsabelDi Masi, Susana NoemiRaba, JulioCalvente, VivianaPenicillium expansumPodredumbresControl BiológicoPatulinaTecnología PostcosechaRotsBiological ControlPatulinPostharvest TechnologyKosakonia radicincitansCryptococcus laurentiiIn the present work, we evaluated the effects of a mixture of biocontrol agents against two toxigenic strains of Penicillium expansum isolated in Argentine Patagonia from pome fruits. The two strains, INTA-5 and INTA-10, were previusly selected among ten strains coming from the Alto Valle (Rio Negro-Argentina) for their high production of patulin. For the biocontrol, Kosakonia radicincitans, Cryptococcus laurentii, and Rhodosporidium fluviale were tested in vitro experiments on Potato Dextrose Agar (PDA) dishes against the INTA-5 and INTA-10 strains. The bacterium K. radicincitans and the yeast C. laurentii were selected to be used in a mixture due to their capacity to control the fungus and reduce the mycotoxin severely. In vitro assays with the mixture showed a high antagonism against P. expansum INTA-5 and INTA-10, at 21 d of incubation at 25 °C and a patulin reduction of 98%. The mixture of microorganisms was also effective in apples stored at 25 °C for 10 d and 4 °C for 30 d. At cold storage, the mixture controlled moderately the development of rot and decreased patulin concentration. At 25 °C, the pathogen's optimal growth temperature, the mixture of Biological Control Agent (BCAs) assured both the control of rot and decrease of patulin concentration. The combination of two microorganisms, with different requirements and abilities, resulted in a mix with a strong antagonism against P. expansum with the capability to decrease the patulin concentration. Treatment with the selected mixture could be a good option for controlling strains with different behaviours and in different environmental conditions.EEA Alto ValleFil: Lambrese, Yesica. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; ArgentinaFil: Lambrese, Yesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina.Fil: Lambrese, Yesica. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; ArgentinaFil: Sansone, Gabriela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; ArgentinaFil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; ArgentinaFil: Sanz Ferramola, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; ArgentinaFil: Di Masi, Susana Noemi. Instituto Nacional Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; ArgentinaFil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; ArgentinaFil: Raba, Julio. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; ArgentinaFil: Calvente, Viviana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2022-11-122021-11-12T17:36:29Z2021-11-12T17:36:29Z2021-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10768https://www.sciencedirect.com/science/article/abs/pii/S07400020210012830740-0020https://doi.org/10.1016/j.fm.2021.103863Food Microbiology 100 : 103863 (December 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:24Zoai:localhost:20.500.12123/10768instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:24.976INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Kosakonia radicincitans and Cryptococcus laurentii controlled Penicillium expansum rot and decreased patulin production at 4 and 25 °C
title Kosakonia radicincitans and Cryptococcus laurentii controlled Penicillium expansum rot and decreased patulin production at 4 and 25 °C
spellingShingle Kosakonia radicincitans and Cryptococcus laurentii controlled Penicillium expansum rot and decreased patulin production at 4 and 25 °C
Lambrese, Yesica
Penicillium expansum
Podredumbres
Control Biológico
Patulina
Tecnología Postcosecha
Rots
Biological Control
Patulin
Postharvest Technology
Kosakonia radicincitans
Cryptococcus laurentii
title_short Kosakonia radicincitans and Cryptococcus laurentii controlled Penicillium expansum rot and decreased patulin production at 4 and 25 °C
title_full Kosakonia radicincitans and Cryptococcus laurentii controlled Penicillium expansum rot and decreased patulin production at 4 and 25 °C
title_fullStr Kosakonia radicincitans and Cryptococcus laurentii controlled Penicillium expansum rot and decreased patulin production at 4 and 25 °C
title_full_unstemmed Kosakonia radicincitans and Cryptococcus laurentii controlled Penicillium expansum rot and decreased patulin production at 4 and 25 °C
title_sort Kosakonia radicincitans and Cryptococcus laurentii controlled Penicillium expansum rot and decreased patulin production at 4 and 25 °C
dc.creator.none.fl_str_mv Lambrese, Yesica
Sansone, Gabriela
Sanz Ferramola, Maria Isabel
Di Masi, Susana Noemi
Raba, Julio
Calvente, Viviana
author Lambrese, Yesica
author_facet Lambrese, Yesica
Sansone, Gabriela
Sanz Ferramola, Maria Isabel
Di Masi, Susana Noemi
Raba, Julio
Calvente, Viviana
author_role author
author2 Sansone, Gabriela
Sanz Ferramola, Maria Isabel
Di Masi, Susana Noemi
Raba, Julio
Calvente, Viviana
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Penicillium expansum
Podredumbres
Control Biológico
Patulina
Tecnología Postcosecha
Rots
Biological Control
Patulin
Postharvest Technology
Kosakonia radicincitans
Cryptococcus laurentii
topic Penicillium expansum
Podredumbres
Control Biológico
Patulina
Tecnología Postcosecha
Rots
Biological Control
Patulin
Postharvest Technology
Kosakonia radicincitans
Cryptococcus laurentii
dc.description.none.fl_txt_mv In the present work, we evaluated the effects of a mixture of biocontrol agents against two toxigenic strains of Penicillium expansum isolated in Argentine Patagonia from pome fruits. The two strains, INTA-5 and INTA-10, were previusly selected among ten strains coming from the Alto Valle (Rio Negro-Argentina) for their high production of patulin. For the biocontrol, Kosakonia radicincitans, Cryptococcus laurentii, and Rhodosporidium fluviale were tested in vitro experiments on Potato Dextrose Agar (PDA) dishes against the INTA-5 and INTA-10 strains. The bacterium K. radicincitans and the yeast C. laurentii were selected to be used in a mixture due to their capacity to control the fungus and reduce the mycotoxin severely. In vitro assays with the mixture showed a high antagonism against P. expansum INTA-5 and INTA-10, at 21 d of incubation at 25 °C and a patulin reduction of 98%. The mixture of microorganisms was also effective in apples stored at 25 °C for 10 d and 4 °C for 30 d. At cold storage, the mixture controlled moderately the development of rot and decreased patulin concentration. At 25 °C, the pathogen's optimal growth temperature, the mixture of Biological Control Agent (BCAs) assured both the control of rot and decrease of patulin concentration. The combination of two microorganisms, with different requirements and abilities, resulted in a mix with a strong antagonism against P. expansum with the capability to decrease the patulin concentration. Treatment with the selected mixture could be a good option for controlling strains with different behaviours and in different environmental conditions.
EEA Alto Valle
Fil: Lambrese, Yesica. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; Argentina
Fil: Lambrese, Yesica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina.
Fil: Lambrese, Yesica. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Sansone, Gabriela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; Argentina
Fil: Sanz Ferramola, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Sanz Ferramola, Maria Isabel. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Di Masi, Susana Noemi. Instituto Nacional Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Alto Valle; Argentina
Fil: Raba, Julio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina
Fil: Raba, Julio. Universidad Nacional de San Luis. Departamento de Química. Instituto de Química de San Luis; Argentina
Fil: Calvente, Viviana. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Química. Área de Tecnología Química y Biotecnología; Argentina
description In the present work, we evaluated the effects of a mixture of biocontrol agents against two toxigenic strains of Penicillium expansum isolated in Argentine Patagonia from pome fruits. The two strains, INTA-5 and INTA-10, were previusly selected among ten strains coming from the Alto Valle (Rio Negro-Argentina) for their high production of patulin. For the biocontrol, Kosakonia radicincitans, Cryptococcus laurentii, and Rhodosporidium fluviale were tested in vitro experiments on Potato Dextrose Agar (PDA) dishes against the INTA-5 and INTA-10 strains. The bacterium K. radicincitans and the yeast C. laurentii were selected to be used in a mixture due to their capacity to control the fungus and reduce the mycotoxin severely. In vitro assays with the mixture showed a high antagonism against P. expansum INTA-5 and INTA-10, at 21 d of incubation at 25 °C and a patulin reduction of 98%. The mixture of microorganisms was also effective in apples stored at 25 °C for 10 d and 4 °C for 30 d. At cold storage, the mixture controlled moderately the development of rot and decreased patulin concentration. At 25 °C, the pathogen's optimal growth temperature, the mixture of Biological Control Agent (BCAs) assured both the control of rot and decrease of patulin concentration. The combination of two microorganisms, with different requirements and abilities, resulted in a mix with a strong antagonism against P. expansum with the capability to decrease the patulin concentration. Treatment with the selected mixture could be a good option for controlling strains with different behaviours and in different environmental conditions.
publishDate 2021
dc.date.none.fl_str_mv 2021-11-12T17:36:29Z
2021-11-12T17:36:29Z
2021-12
info:eu-repo/date/embargoEnd/2022-11-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10768
https://www.sciencedirect.com/science/article/abs/pii/S0740002021001283
0740-0020
https://doi.org/10.1016/j.fm.2021.103863
url http://hdl.handle.net/20.500.12123/10768
https://www.sciencedirect.com/science/article/abs/pii/S0740002021001283
https://doi.org/10.1016/j.fm.2021.103863
identifier_str_mv 0740-0020
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Food Microbiology 100 : 103863 (December 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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