Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents

Autores
Magnoli, Alejandra Paola; Rodriguez Romera, Mariela Carmen; Poloni, V.L.; Combina, Mariana; Chiacchiera, Sebastián; Dalcero, Ana María; Cavaglieri, Lilia Reneé; Rojo, María Cecilia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Aims: To isolate and characterize native yeast strains from broilers’ environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1). Methods and Results: A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), two from gut identified as Candida tropicalis and four from faeces identified as Cl. lusitaniae (3) and Cyberlindnera fabianii (1). AFB1 binding percentages varied among yeast strains and with AFB1 concentrations. To carry out adsorption studies, one strain from each genus and each origin was selected as follows: Cl. lusitaniae and P. kudriavzevii from feedstuff, Cl. lusitaniae and Cy. fabianii from faeces and Ca. tropicalis from gut. The most appropriate concentrations for cells and toxin were 107 cells per ml and 100 ng ml _1 of AFB1 respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherm studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the AFB1–yeast complex demonstrated the irreversibility of the binding process. Conclusion: Yeast strains tested in this study constitute potential AFB1 adsorbents and they possess the advantage to be native from the avian environment. Significance and Impact of the Study: This study makes a contribution to using native yeasts from broilers’ environment for controlling chronic aflatoxicosis in avian production.
EEA Mendoza
Fil: Magnoli, Alejandra Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Rodriguez Romera, Mariela Carmen. Universidad Nacional de Río Cuarto. Departamento de Microbiología e Inmunología; Argentina
Fil: Poloni, V.L. Universidad Nacional de Río Cuarto. Departamento de Microbiología e Inmunología; Argentina
Fil: Combina, Mariana.Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Estudios Enológicos; Argentina
Fil: Chiacchiera, Sebastián. Estación Experimental Agropecuaria Marcos Juarez; Argentina
Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rojo, María Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of applied microbiology 121 (6) : 1766–1776. (December 2016)
Materia
Pollo de Engorde
Levadura
Piensos
Aflatoxinas
Broiler Chickens
Yeasts
Feeds
Aflatoxins
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1401
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1401
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbentsMagnoli, Alejandra PaolaRodriguez Romera, Mariela CarmenPoloni, V.L.Combina, MarianaChiacchiera, SebastiánDalcero, Ana MaríaCavaglieri, Lilia ReneéRojo, María CeciliaPollo de EngordeLevaduraPiensosAflatoxinasBroiler ChickensYeastsFeedsAflatoxinsAims: To isolate and characterize native yeast strains from broilers’ environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1). Methods and Results: A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), two from gut identified as Candida tropicalis and four from faeces identified as Cl. lusitaniae (3) and Cyberlindnera fabianii (1). AFB1 binding percentages varied among yeast strains and with AFB1 concentrations. To carry out adsorption studies, one strain from each genus and each origin was selected as follows: Cl. lusitaniae and P. kudriavzevii from feedstuff, Cl. lusitaniae and Cy. fabianii from faeces and Ca. tropicalis from gut. The most appropriate concentrations for cells and toxin were 107 cells per ml and 100 ng ml _1 of AFB1 respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherm studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the AFB1–yeast complex demonstrated the irreversibility of the binding process. Conclusion: Yeast strains tested in this study constitute potential AFB1 adsorbents and they possess the advantage to be native from the avian environment. Significance and Impact of the Study: This study makes a contribution to using native yeasts from broilers’ environment for controlling chronic aflatoxicosis in avian production.EEA MendozaFil: Magnoli, Alejandra Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Rodriguez Romera, Mariela Carmen. Universidad Nacional de Río Cuarto. Departamento de Microbiología e Inmunología; ArgentinaFil: Poloni, V.L. Universidad Nacional de Río Cuarto. Departamento de Microbiología e Inmunología; ArgentinaFil: Combina, Mariana.Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Estudios Enológicos; ArgentinaFil: Chiacchiera, Sebastián. Estación Experimental Agropecuaria Marcos Juarez; ArgentinaFil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rojo, María Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2017-10-04T13:11:25Z2017-10-04T13:11:25Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1401http://onlinelibrary.wiley.com/doi/10.1111/jam.13297/abstract1364-5072 (Print)1365-2672 (Online)DOI: 10.1111/jam.13297Journal of applied microbiology 121 (6) : 1766–1776. (December 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:12Zoai:localhost:20.500.12123/1401instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:12.338INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents
title Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents
spellingShingle Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents
Magnoli, Alejandra Paola
Pollo de Engorde
Levadura
Piensos
Aflatoxinas
Broiler Chickens
Yeasts
Feeds
Aflatoxins
title_short Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents
title_full Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents
title_fullStr Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents
title_full_unstemmed Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents
title_sort Novel yeast isolated from broilers feedstuff, gut and faeces as aflatoxin 1 B1 adsorbents
dc.creator.none.fl_str_mv Magnoli, Alejandra Paola
Rodriguez Romera, Mariela Carmen
Poloni, V.L.
Combina, Mariana
Chiacchiera, Sebastián
Dalcero, Ana María
Cavaglieri, Lilia Reneé
Rojo, María Cecilia
author Magnoli, Alejandra Paola
author_facet Magnoli, Alejandra Paola
Rodriguez Romera, Mariela Carmen
Poloni, V.L.
Combina, Mariana
Chiacchiera, Sebastián
Dalcero, Ana María
Cavaglieri, Lilia Reneé
Rojo, María Cecilia
author_role author
author2 Rodriguez Romera, Mariela Carmen
Poloni, V.L.
Combina, Mariana
Chiacchiera, Sebastián
Dalcero, Ana María
Cavaglieri, Lilia Reneé
Rojo, María Cecilia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Pollo de Engorde
Levadura
Piensos
Aflatoxinas
Broiler Chickens
Yeasts
Feeds
Aflatoxins
topic Pollo de Engorde
Levadura
Piensos
Aflatoxinas
Broiler Chickens
Yeasts
Feeds
Aflatoxins
dc.description.none.fl_txt_mv Aims: To isolate and characterize native yeast strains from broilers’ environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1). Methods and Results: A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), two from gut identified as Candida tropicalis and four from faeces identified as Cl. lusitaniae (3) and Cyberlindnera fabianii (1). AFB1 binding percentages varied among yeast strains and with AFB1 concentrations. To carry out adsorption studies, one strain from each genus and each origin was selected as follows: Cl. lusitaniae and P. kudriavzevii from feedstuff, Cl. lusitaniae and Cy. fabianii from faeces and Ca. tropicalis from gut. The most appropriate concentrations for cells and toxin were 107 cells per ml and 100 ng ml _1 of AFB1 respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherm studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the AFB1–yeast complex demonstrated the irreversibility of the binding process. Conclusion: Yeast strains tested in this study constitute potential AFB1 adsorbents and they possess the advantage to be native from the avian environment. Significance and Impact of the Study: This study makes a contribution to using native yeasts from broilers’ environment for controlling chronic aflatoxicosis in avian production.
EEA Mendoza
Fil: Magnoli, Alejandra Paola. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Rodriguez Romera, Mariela Carmen. Universidad Nacional de Río Cuarto. Departamento de Microbiología e Inmunología; Argentina
Fil: Poloni, V.L. Universidad Nacional de Río Cuarto. Departamento de Microbiología e Inmunología; Argentina
Fil: Combina, Mariana.Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza. Centro de Estudios Enológicos; Argentina
Fil: Chiacchiera, Sebastián. Estación Experimental Agropecuaria Marcos Juarez; Argentina
Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rojo, María Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Aims: To isolate and characterize native yeast strains from broilers’ environment as feedstuff, faeces and gut, and to evaluate their binding capacity for aflatoxin B1 (AFB1). Methods and Results: A total of nine yeast strains were isolated: three from feedstuff identified as Pichia kudriavzevii (2) and Clavispora lusitaniae (1), two from gut identified as Candida tropicalis and four from faeces identified as Cl. lusitaniae (3) and Cyberlindnera fabianii (1). AFB1 binding percentages varied among yeast strains and with AFB1 concentrations. To carry out adsorption studies, one strain from each genus and each origin was selected as follows: Cl. lusitaniae and P. kudriavzevii from feedstuff, Cl. lusitaniae and Cy. fabianii from faeces and Ca. tropicalis from gut. The most appropriate concentrations for cells and toxin were 107 cells per ml and 100 ng ml _1 of AFB1 respectively. All the tested yeast strains showed similar adsorption capacities independently of the origin. The adsorption isotherm studies in all yeasts assayed showed behaviour of L type or Langmuir and a varied affinity for the toxin. The stability of the AFB1–yeast complex demonstrated the irreversibility of the binding process. Conclusion: Yeast strains tested in this study constitute potential AFB1 adsorbents and they possess the advantage to be native from the avian environment. Significance and Impact of the Study: This study makes a contribution to using native yeasts from broilers’ environment for controlling chronic aflatoxicosis in avian production.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-10-04T13:11:25Z
2017-10-04T13:11:25Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1401
http://onlinelibrary.wiley.com/doi/10.1111/jam.13297/abstract
1364-5072 (Print)
1365-2672 (Online)
DOI: 10.1111/jam.13297
url http://hdl.handle.net/20.500.12123/1401
http://onlinelibrary.wiley.com/doi/10.1111/jam.13297/abstract
identifier_str_mv 1364-5072 (Print)
1365-2672 (Online)
DOI: 10.1111/jam.13297
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of applied microbiology 121 (6) : 1766–1776. (December 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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