Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens

Autores
Zanetti, Flavia Adriana; Cardona, Romina; Federico, Carlos Rodolfo; Chimeno Zoth, Silvina Andrea; Calamante, Gabriela
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′).
Inst. de Biotecnología
Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Cardona, Romina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Federico, Carlos Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas: Argentina
Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Virologica sinica 31(3) : 266–269. (June 2016)
Materia
Enfermedades de los Animales
Pollo
Virus de los Animales
Chickens
Animal Diseases
Animal Viruses
Canarypox
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1051
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1051
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickensZanetti, Flavia AdrianaCardona, RominaFederico, Carlos RodolfoChimeno Zoth, Silvina AndreaCalamante, GabrielaEnfermedades de los AnimalesPolloVirus de los AnimalesChickensAnimal DiseasesAnimal VirusesCanarypoxTo study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′).Inst. de BiotecnologíaFil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Cardona, Romina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Federico, Carlos Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas: ArgentinaFil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina2017-08-28T13:20:59Z2017-08-28T13:20:59Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1051https://link.springer.com/article/10.1007%2Fs12250-015-3680-61995-820X (Online)1674-0769 (Print)https://doi.org/10.1007/s12250-015-3680-6Virologica sinica 31(3) : 266–269. (June 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:09Zoai:localhost:20.500.12123/1051instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:09.508INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens
title Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens
spellingShingle Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens
Zanetti, Flavia Adriana
Enfermedades de los Animales
Pollo
Virus de los Animales
Chickens
Animal Diseases
Animal Viruses
Canarypox
title_short Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens
title_full Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens
title_fullStr Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens
title_full_unstemmed Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens
title_sort Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens
dc.creator.none.fl_str_mv Zanetti, Flavia Adriana
Cardona, Romina
Federico, Carlos Rodolfo
Chimeno Zoth, Silvina Andrea
Calamante, Gabriela
author Zanetti, Flavia Adriana
author_facet Zanetti, Flavia Adriana
Cardona, Romina
Federico, Carlos Rodolfo
Chimeno Zoth, Silvina Andrea
Calamante, Gabriela
author_role author
author2 Cardona, Romina
Federico, Carlos Rodolfo
Chimeno Zoth, Silvina Andrea
Calamante, Gabriela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Pollo
Virus de los Animales
Chickens
Animal Diseases
Animal Viruses
Canarypox
topic Enfermedades de los Animales
Pollo
Virus de los Animales
Chickens
Animal Diseases
Animal Viruses
Canarypox
dc.description.none.fl_txt_mv To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′).
Inst. de Biotecnología
Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Cardona, Romina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Federico, Carlos Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas: Argentina
Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′).
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-08-28T13:20:59Z
2017-08-28T13:20:59Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1051
https://link.springer.com/article/10.1007%2Fs12250-015-3680-6
1995-820X (Online)
1674-0769 (Print)
https://doi.org/10.1007/s12250-015-3680-6
url http://hdl.handle.net/20.500.12123/1051
https://link.springer.com/article/10.1007%2Fs12250-015-3680-6
https://doi.org/10.1007/s12250-015-3680-6
identifier_str_mv 1995-820X (Online)
1674-0769 (Print)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Virologica sinica 31(3) : 266–269. (June 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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