Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens
- Autores
- Zanetti, Flavia Adriana; Cardona, Romina; Federico, Carlos Rodolfo; Chimeno Zoth, Silvina Andrea; Calamante, Gabriela
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión aceptada
- Descripción
- To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′).
Inst. de Biotecnología
Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina
Fil: Cardona, Romina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Federico, Carlos Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas: Argentina
Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina - Fuente
- Virologica sinica 31(3) : 266–269. (June 2016)
- Materia
-
Enfermedades de los Animales
Pollo
Virus de los Animales
Chickens
Animal Diseases
Animal Viruses
Canarypox - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/1051
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Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickensZanetti, Flavia AdrianaCardona, RominaFederico, Carlos RodolfoChimeno Zoth, Silvina AndreaCalamante, GabrielaEnfermedades de los AnimalesPolloVirus de los AnimalesChickensAnimal DiseasesAnimal VirusesCanarypoxTo study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′).Inst. de BiotecnologíaFil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; ArgentinaFil: Cardona, Romina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Federico, Carlos Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas: ArgentinaFil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina2017-08-28T13:20:59Z2017-08-28T13:20:59Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1051https://link.springer.com/article/10.1007%2Fs12250-015-3680-61995-820X (Online)1674-0769 (Print)https://doi.org/10.1007/s12250-015-3680-6Virologica sinica 31(3) : 266–269. (June 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:46:58Zoai:localhost:20.500.12123/1051instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:46:58.521INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
title |
Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
spellingShingle |
Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens Zanetti, Flavia Adriana Enfermedades de los Animales Pollo Virus de los Animales Chickens Animal Diseases Animal Viruses Canarypox |
title_short |
Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
title_full |
Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
title_fullStr |
Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
title_full_unstemmed |
Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
title_sort |
Recombinant canarypox virus expressing the VP2 protein of infectious bursal disease virus induces protection in vaccinated SPF chickens |
dc.creator.none.fl_str_mv |
Zanetti, Flavia Adriana Cardona, Romina Federico, Carlos Rodolfo Chimeno Zoth, Silvina Andrea Calamante, Gabriela |
author |
Zanetti, Flavia Adriana |
author_facet |
Zanetti, Flavia Adriana Cardona, Romina Federico, Carlos Rodolfo Chimeno Zoth, Silvina Andrea Calamante, Gabriela |
author_role |
author |
author2 |
Cardona, Romina Federico, Carlos Rodolfo Chimeno Zoth, Silvina Andrea Calamante, Gabriela |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Enfermedades de los Animales Pollo Virus de los Animales Chickens Animal Diseases Animal Viruses Canarypox |
topic |
Enfermedades de los Animales Pollo Virus de los Animales Chickens Animal Diseases Animal Viruses Canarypox |
dc.description.none.fl_txt_mv |
To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′). Inst. de Biotecnología Fil: Zanetti, Flavia Adriana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Ciencias y Tecnología "Dr. Cesar Milstein"; Argentina Fil: Cardona, Romina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Federico, Carlos Rodolfo. Consejo Nacional de Investigaciones Científicas y Técnicas: Argentina Fil: Chimeno Zoth, Silvina Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Calamante, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina |
description |
To study the in vivo distribution of the CNPV viral vector, we conducted two trials. In the first trial, six 11- day-old SPF chickens were intramuscularly vaccinated in one leg with CN048-VP2 (106 plaque-forming units (PFU) mixed with 0.5% v/v Indian ink). Three birds were sacrificed at 1 and 3 days after vaccination, and muscle at the immunization site (identified by Indian ink), muscle from the non-injected leg, BF, and spleen were removed, weighed, and homogenized in phosphate buffer (50% w/v). Total DNA was extracted from organs using a QIAamp® DNA Mini Kit (QIAGEN, USA) and used as the template for PCR amplification of CNPV241 (primers P39F: 5′-AATAACACGACACAGCCGCAAG-3′, P39R: 5′-CAATTAATTAGATCGTGGTGGA-3′) or the glyceraldehyde-3-phosphate dehydrogenase (gapdh) gene (primers GAPDH Fw: 5′-AGAACATCATCCCAGCGTCC-3′, GAPDH Rv: 5′-CGGCAGGTCAGGTCAACA-3′). |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2017-08-28T13:20:59Z 2017-08-28T13:20:59Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
acceptedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/1051 https://link.springer.com/article/10.1007%2Fs12250-015-3680-6 1995-820X (Online) 1674-0769 (Print) https://doi.org/10.1007/s12250-015-3680-6 |
url |
http://hdl.handle.net/20.500.12123/1051 https://link.springer.com/article/10.1007%2Fs12250-015-3680-6 https://doi.org/10.1007/s12250-015-3680-6 |
identifier_str_mv |
1995-820X (Online) 1674-0769 (Print) |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Virologica sinica 31(3) : 266–269. (June 2016) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1842341349355945984 |
score |
12.623145 |