A plasmid encoding the extracellular domain of CD40 ligand and MontanideTM GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1
- Autores
- Kornuta, Claudia Alejandra; Langellotti, Cecilia Ana; Bidart, , Juan Esteban; Soria, Ivana; Quattrocchi, Valeria; Gammella, Mariela; Cheuquepan Valenzuela, Felipe; Mignaqui, Ana Clara; Ferraris, Sergio; Charleston, Bryan; Hecker, Yanina; Moore, Prando Dadin; Zamorano, Patricia Ines
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- DNA vaccines are capable of inducing humoral and cellular immunity, and are important to control bovine herpesvirus 1 (BoHV-1), an agent of the bovine respiratory disease complex. In previous work, a DNA plasmid that encodes a secreted form of BoHV-1 glycoprotein D (pCIgD) together with commercial adjuvants provided partial protection against viral challenge of bovines. In this work, we evaluate new molecules that could potentiate the DNA vaccine. We show that a plasmid encoding a soluble CD40 ligand (CD40L) and the adjuvant Montanide™ GEL01 (GEL01) activate in vitro bovine afferent lymph dendritic cells (ALDCs). CD40L is a co-stimulating molecule, expressed transiently on activated CD4+ T cells and, to a lesser extent, on activated B cells and platelets. The interaction with its receptor, CD40, exerts effects on the presenting cells, triggering responses in the immune system. GEL01 was designed to improve transfection of DNA vaccines. We vaccinated cattle with: pCIgD; pCIgD-GEL01; pCIgD with GEL01 and CD40L plasmid (named pCIgD-CD40L-GEL01) or with pCIneo vaccines. The results show that CD40L plasmid with GEL01 improved the pCIgD DNA vaccine, increasing anti-BoHV-1 total IgGs, IgG1, IgG2 subclasses, and neutralizing antibodies in serum. After viral challenge, bovines vaccinated with pCIgD-GEL01-CD40L showed a significant decrease in viral excretion and clinical score. On the other hand, 80% of animals in group pCIgD-GEL01-CD40L presented specific anti-BoHV-1 IgG1 antibodies in nasal swabs. In addition, PBMCs from pCIgD-CD40L-GEL01 had the highest percentage of animals with a positive lymphoproliferative response against the virus and significant differences in the secretion of IFNγ and IL-4 by mononuclear cells, indicating the stimulation of the cellular immune response. Overall, the results demonstrate that a plasmid expressing CD40L associated with the adjuvant GEL01 improves the efficacy of a DNA vaccine against BoHV-1.
Instituto de Virología
Fil: Kornuta, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bidart, Juan Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cheuquepan Valenzuela, Felipe. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Ferraris, Sergio. Universidad Maimónides; Argentina
Fil: Charleston, Bryan. Pirbright Institute; Reino Unido
Fil: Hecker, Yanina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moore, Dadin P. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; Argentina - Fuente
- Vaccine 39 (6) : 1007-1017 (Febrero 2021)
- Materia
-
Bovine Herpesvirus
Bovinae
Vaccine Adjuvants
Immunogenetics
Herpes Virus Bovino
Bovina
Coadyuvantes de Vacunas
Inmunogenética - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/8681
Ver los metadatos del registro completo
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A plasmid encoding the extracellular domain of CD40 ligand and MontanideTM GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1Kornuta, Claudia AlejandraLangellotti, Cecilia AnaBidart, , Juan EstebanSoria, IvanaQuattrocchi, ValeriaGammella, MarielaCheuquepan Valenzuela, FelipeMignaqui, Ana ClaraFerraris, SergioCharleston, BryanHecker, YaninaMoore, Prando DadinZamorano, Patricia InesBovine HerpesvirusBovinaeVaccine AdjuvantsImmunogeneticsHerpes Virus BovinoBovinaCoadyuvantes de VacunasInmunogenéticaDNA vaccines are capable of inducing humoral and cellular immunity, and are important to control bovine herpesvirus 1 (BoHV-1), an agent of the bovine respiratory disease complex. In previous work, a DNA plasmid that encodes a secreted form of BoHV-1 glycoprotein D (pCIgD) together with commercial adjuvants provided partial protection against viral challenge of bovines. In this work, we evaluate new molecules that could potentiate the DNA vaccine. We show that a plasmid encoding a soluble CD40 ligand (CD40L) and the adjuvant Montanide™ GEL01 (GEL01) activate in vitro bovine afferent lymph dendritic cells (ALDCs). CD40L is a co-stimulating molecule, expressed transiently on activated CD4+ T cells and, to a lesser extent, on activated B cells and platelets. The interaction with its receptor, CD40, exerts effects on the presenting cells, triggering responses in the immune system. GEL01 was designed to improve transfection of DNA vaccines. We vaccinated cattle with: pCIgD; pCIgD-GEL01; pCIgD with GEL01 and CD40L plasmid (named pCIgD-CD40L-GEL01) or with pCIneo vaccines. The results show that CD40L plasmid with GEL01 improved the pCIgD DNA vaccine, increasing anti-BoHV-1 total IgGs, IgG1, IgG2 subclasses, and neutralizing antibodies in serum. After viral challenge, bovines vaccinated with pCIgD-GEL01-CD40L showed a significant decrease in viral excretion and clinical score. On the other hand, 80% of animals in group pCIgD-GEL01-CD40L presented specific anti-BoHV-1 IgG1 antibodies in nasal swabs. In addition, PBMCs from pCIgD-CD40L-GEL01 had the highest percentage of animals with a positive lymphoproliferative response against the virus and significant differences in the secretion of IFNγ and IL-4 by mononuclear cells, indicating the stimulation of the cellular immune response. Overall, the results demonstrate that a plasmid expressing CD40L associated with the adjuvant GEL01 improves the efficacy of a DNA vaccine against BoHV-1.Instituto de VirologíaFil: Kornuta, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bidart, Juan Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cheuquepan Valenzuela, Felipe. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas; ArgentinaFil: Ferraris, Sergio. Universidad Maimónides; ArgentinaFil: Charleston, Bryan. Pirbright Institute; Reino UnidoFil: Hecker, Yanina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moore, Dadin P. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; ArgentinaElsevier2021-02-18T12:31:04Z2021-02-18T12:31:04Z2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8681https://www.sciencedirect.com/science/article/pii/S0264410X2031553X0264-410Xhttps://doi.org/10.1016/j.vaccine.2020.11.071Vaccine 39 (6) : 1007-1017 (Febrero 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO-1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular.info:eu-repo/semantics/restrictedAccess2025-09-29T13:45:08Zoai:localhost:20.500.12123/8681instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:08.421INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
A plasmid encoding the extracellular domain of CD40 ligand and MontanideTM GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1 |
| title |
A plasmid encoding the extracellular domain of CD40 ligand and MontanideTM GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1 |
| spellingShingle |
A plasmid encoding the extracellular domain of CD40 ligand and MontanideTM GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1 Kornuta, Claudia Alejandra Bovine Herpesvirus Bovinae Vaccine Adjuvants Immunogenetics Herpes Virus Bovino Bovina Coadyuvantes de Vacunas Inmunogenética |
| title_short |
A plasmid encoding the extracellular domain of CD40 ligand and MontanideTM GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1 |
| title_full |
A plasmid encoding the extracellular domain of CD40 ligand and MontanideTM GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1 |
| title_fullStr |
A plasmid encoding the extracellular domain of CD40 ligand and MontanideTM GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1 |
| title_full_unstemmed |
A plasmid encoding the extracellular domain of CD40 ligand and MontanideTM GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1 |
| title_sort |
A plasmid encoding the extracellular domain of CD40 ligand and MontanideTM GEL01 as adjuvants enhance the immunogenicity and the protection induced by a DNA vaccine against BoHV-1 |
| dc.creator.none.fl_str_mv |
Kornuta, Claudia Alejandra Langellotti, Cecilia Ana Bidart, , Juan Esteban Soria, Ivana Quattrocchi, Valeria Gammella, Mariela Cheuquepan Valenzuela, Felipe Mignaqui, Ana Clara Ferraris, Sergio Charleston, Bryan Hecker, Yanina Moore, Prando Dadin Zamorano, Patricia Ines |
| author |
Kornuta, Claudia Alejandra |
| author_facet |
Kornuta, Claudia Alejandra Langellotti, Cecilia Ana Bidart, , Juan Esteban Soria, Ivana Quattrocchi, Valeria Gammella, Mariela Cheuquepan Valenzuela, Felipe Mignaqui, Ana Clara Ferraris, Sergio Charleston, Bryan Hecker, Yanina Moore, Prando Dadin Zamorano, Patricia Ines |
| author_role |
author |
| author2 |
Langellotti, Cecilia Ana Bidart, , Juan Esteban Soria, Ivana Quattrocchi, Valeria Gammella, Mariela Cheuquepan Valenzuela, Felipe Mignaqui, Ana Clara Ferraris, Sergio Charleston, Bryan Hecker, Yanina Moore, Prando Dadin Zamorano, Patricia Ines |
| author2_role |
author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Bovine Herpesvirus Bovinae Vaccine Adjuvants Immunogenetics Herpes Virus Bovino Bovina Coadyuvantes de Vacunas Inmunogenética |
| topic |
Bovine Herpesvirus Bovinae Vaccine Adjuvants Immunogenetics Herpes Virus Bovino Bovina Coadyuvantes de Vacunas Inmunogenética |
| dc.description.none.fl_txt_mv |
DNA vaccines are capable of inducing humoral and cellular immunity, and are important to control bovine herpesvirus 1 (BoHV-1), an agent of the bovine respiratory disease complex. In previous work, a DNA plasmid that encodes a secreted form of BoHV-1 glycoprotein D (pCIgD) together with commercial adjuvants provided partial protection against viral challenge of bovines. In this work, we evaluate new molecules that could potentiate the DNA vaccine. We show that a plasmid encoding a soluble CD40 ligand (CD40L) and the adjuvant Montanide™ GEL01 (GEL01) activate in vitro bovine afferent lymph dendritic cells (ALDCs). CD40L is a co-stimulating molecule, expressed transiently on activated CD4+ T cells and, to a lesser extent, on activated B cells and platelets. The interaction with its receptor, CD40, exerts effects on the presenting cells, triggering responses in the immune system. GEL01 was designed to improve transfection of DNA vaccines. We vaccinated cattle with: pCIgD; pCIgD-GEL01; pCIgD with GEL01 and CD40L plasmid (named pCIgD-CD40L-GEL01) or with pCIneo vaccines. The results show that CD40L plasmid with GEL01 improved the pCIgD DNA vaccine, increasing anti-BoHV-1 total IgGs, IgG1, IgG2 subclasses, and neutralizing antibodies in serum. After viral challenge, bovines vaccinated with pCIgD-GEL01-CD40L showed a significant decrease in viral excretion and clinical score. On the other hand, 80% of animals in group pCIgD-GEL01-CD40L presented specific anti-BoHV-1 IgG1 antibodies in nasal swabs. In addition, PBMCs from pCIgD-CD40L-GEL01 had the highest percentage of animals with a positive lymphoproliferative response against the virus and significant differences in the secretion of IFNγ and IL-4 by mononuclear cells, indicating the stimulation of the cellular immune response. Overall, the results demonstrate that a plasmid expressing CD40L associated with the adjuvant GEL01 improves the efficacy of a DNA vaccine against BoHV-1. Instituto de Virología Fil: Kornuta, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bidart, Juan Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Cheuquepan Valenzuela, Felipe. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Mignaqui, Ana Clara. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina Fil: Ferraris, Sergio. Universidad Maimónides; Argentina Fil: Charleston, Bryan. Pirbright Institute; Reino Unido Fil: Hecker, Yanina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Moore, Dadin P. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad del Salvador; Argentina |
| description |
DNA vaccines are capable of inducing humoral and cellular immunity, and are important to control bovine herpesvirus 1 (BoHV-1), an agent of the bovine respiratory disease complex. In previous work, a DNA plasmid that encodes a secreted form of BoHV-1 glycoprotein D (pCIgD) together with commercial adjuvants provided partial protection against viral challenge of bovines. In this work, we evaluate new molecules that could potentiate the DNA vaccine. We show that a plasmid encoding a soluble CD40 ligand (CD40L) and the adjuvant Montanide™ GEL01 (GEL01) activate in vitro bovine afferent lymph dendritic cells (ALDCs). CD40L is a co-stimulating molecule, expressed transiently on activated CD4+ T cells and, to a lesser extent, on activated B cells and platelets. The interaction with its receptor, CD40, exerts effects on the presenting cells, triggering responses in the immune system. GEL01 was designed to improve transfection of DNA vaccines. We vaccinated cattle with: pCIgD; pCIgD-GEL01; pCIgD with GEL01 and CD40L plasmid (named pCIgD-CD40L-GEL01) or with pCIneo vaccines. The results show that CD40L plasmid with GEL01 improved the pCIgD DNA vaccine, increasing anti-BoHV-1 total IgGs, IgG1, IgG2 subclasses, and neutralizing antibodies in serum. After viral challenge, bovines vaccinated with pCIgD-GEL01-CD40L showed a significant decrease in viral excretion and clinical score. On the other hand, 80% of animals in group pCIgD-GEL01-CD40L presented specific anti-BoHV-1 IgG1 antibodies in nasal swabs. In addition, PBMCs from pCIgD-CD40L-GEL01 had the highest percentage of animals with a positive lymphoproliferative response against the virus and significant differences in the secretion of IFNγ and IL-4 by mononuclear cells, indicating the stimulation of the cellular immune response. Overall, the results demonstrate that a plasmid expressing CD40L associated with the adjuvant GEL01 improves the efficacy of a DNA vaccine against BoHV-1. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-02-18T12:31:04Z 2021-02-18T12:31:04Z 2021-02 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
| format |
article |
| status_str |
publishedVersion |
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http://hdl.handle.net/20.500.12123/8681 https://www.sciencedirect.com/science/article/pii/S0264410X2031553X 0264-410X https://doi.org/10.1016/j.vaccine.2020.11.071 |
| url |
http://hdl.handle.net/20.500.12123/8681 https://www.sciencedirect.com/science/article/pii/S0264410X2031553X https://doi.org/10.1016/j.vaccine.2020.11.071 |
| identifier_str_mv |
0264-410X |
| dc.language.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
info:eu-repograntAgreement/INTA/PNBIO-1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular. |
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application/pdf |
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Elsevier |
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Elsevier |
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Vaccine 39 (6) : 1007-1017 (Febrero 2021) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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