MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1

Autores
Kornuta, Claudia Alejandra; Bidart, , Juan Esteban; Soria, Ivana; Gammella, Mariela; Quattrocchi, Valeria; Pappalardo, Juan Sebastián; Salmaso, Stefano; Torchilin, Vladimir P.; Cheuquepan Valenzuel, Felipe Andres; Hecker, Yanina; Moore, Dadin P.; Zamorano, Patricia Ines; Langellotti, Cecilia Ana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
New technologies in the field of vaccinology arise as a necessity for the treatment and control of many diseases. Whole virus inactivated vaccines and modified live virus ones used against Bovine Herpesvirus‐1 (BoHV‐1) infection have several disadvantages. Previous works on DNA vaccines against BoHV‐1 have demonstrated the capability to induce humoral and cellular immune responses. Nevertheless, ‘naked’ DNA induces low immunogenic response. Thus, loading of antigen encoding DNA sequences in liposomal formulations targeting dendritic cell receptors could be a promising strategy to better activate these antigen‐presenting cells (APC). In this work, a DNA‐based vaccine encoding the truncated version of BoHV‐1 glycoprotein D (pCIgD) was evaluated alone and encapsulated in a liposomal formulation containing LPS and decorated with MANα1‐2MAN‐PEG‐DOPE (pCIgD‐Man‐L). The vaccinations were performed in mice and bovines. The results showed that the use of pCIgD‐Man‐L enhanced the immune response in both animal models. For humoral immunity, significant differences were achieved when total antibody titres and isotypes were assayed in sera. Regarding cellular immunity, a significant increase in the proliferative response against BoHV‐1 was detected in animals vaccinated with pCIgD‐Man‐L when compared to the response induced in animals vaccinated with pCIgD. In addition, upregulation of CD40 molecules on the surface of bovine dendritic cells (DCs) was observed when cells were stimulated and activated with the vaccine formulations. When viral challenge was performed, bovines vaccinated with MANα1‐2MAN‐PEG‐DOPE elicited better protection which was evidenced by a lower viral excretion. These results demonstrate that the dendritic cell targeting using MANα1‐2MAN decorated liposomes can boost the immunogenicity resulting in a long‐lasting immunity. Liposomes decorated with MANα1‐2MAN‐PEG‐DOPE were tested for the first time as a DNA vaccine nanovehicle in cattle as a preventive treatment against BoHV‐1. These results open new perspectives for the design of vaccines for the control of bovine rhinotracheitis.
Instituto de Virología
Fil: Kornuta, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Bidart, Juan Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Salmaso, Stefano. Universita degli Studi di Padova. Department of Pharmaceutical and Pharmacological Sciences; Italia
Fil: Torchilin, Vladimir P. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Cheuquepán Valenzuela, Felipe Andrés. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina.
Fil: Hecker, Yanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Moore, Dadin P. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kornuta, Claudia Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Bidart, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Soria, Ivana. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina
Fil: Gammella, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Cheuquepán Valenzuela, Felipe Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Cheuquepán Valenzuela, Felipe Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Zamorano, Patricia Ines. Universidad del Salvador; Argentina
Fil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fuente
Transboundary and emerging diseases 68 (2) : 587-597 (March 2021)
Materia
Liposomes (Organelles)
Liposomas (Organulos)
Inmunogenética
Vacuna
Herpesviridae
Immunogenetics
Vaccines
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8603
Acceder
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network_name_str INTA Digital (INTA)
spelling MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1Kornuta, Claudia AlejandraBidart, , Juan EstebanSoria, IvanaGammella, MarielaQuattrocchi, ValeriaPappalardo, Juan SebastiánSalmaso, StefanoTorchilin, Vladimir P.Cheuquepan Valenzuel, Felipe AndresHecker, YaninaMoore, Dadin P.Zamorano, Patricia InesLangellotti, Cecilia AnaLiposomes (Organelles)Liposomas (Organulos)InmunogenéticaVacunaHerpesviridaeImmunogeneticsVaccinesNew technologies in the field of vaccinology arise as a necessity for the treatment and control of many diseases. Whole virus inactivated vaccines and modified live virus ones used against Bovine Herpesvirus‐1 (BoHV‐1) infection have several disadvantages. Previous works on DNA vaccines against BoHV‐1 have demonstrated the capability to induce humoral and cellular immune responses. Nevertheless, ‘naked’ DNA induces low immunogenic response. Thus, loading of antigen encoding DNA sequences in liposomal formulations targeting dendritic cell receptors could be a promising strategy to better activate these antigen‐presenting cells (APC). In this work, a DNA‐based vaccine encoding the truncated version of BoHV‐1 glycoprotein D (pCIgD) was evaluated alone and encapsulated in a liposomal formulation containing LPS and decorated with MANα1‐2MAN‐PEG‐DOPE (pCIgD‐Man‐L). The vaccinations were performed in mice and bovines. The results showed that the use of pCIgD‐Man‐L enhanced the immune response in both animal models. For humoral immunity, significant differences were achieved when total antibody titres and isotypes were assayed in sera. Regarding cellular immunity, a significant increase in the proliferative response against BoHV‐1 was detected in animals vaccinated with pCIgD‐Man‐L when compared to the response induced in animals vaccinated with pCIgD. In addition, upregulation of CD40 molecules on the surface of bovine dendritic cells (DCs) was observed when cells were stimulated and activated with the vaccine formulations. When viral challenge was performed, bovines vaccinated with MANα1‐2MAN‐PEG‐DOPE elicited better protection which was evidenced by a lower viral excretion. These results demonstrate that the dendritic cell targeting using MANα1‐2MAN decorated liposomes can boost the immunogenicity resulting in a long‐lasting immunity. Liposomes decorated with MANα1‐2MAN‐PEG‐DOPE were tested for the first time as a DNA vaccine nanovehicle in cattle as a preventive treatment against BoHV‐1. These results open new perspectives for the design of vaccines for the control of bovine rhinotracheitis.Instituto de VirologíaFil: Kornuta, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Bidart, Juan Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Salmaso, Stefano. Universita degli Studi di Padova. Department of Pharmaceutical and Pharmacological Sciences; ItaliaFil: Torchilin, Vladimir P. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados UnidosFil: Cheuquepán Valenzuela, Felipe Andrés. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina.Fil: Hecker, Yanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Moore, Dadin P. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); ArgentinaFil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Kornuta, Claudia Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Bidart, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Soria, Ivana. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); ArgentinaFil: Gammella, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Cheuquepán Valenzuela, Felipe Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Cheuquepán Valenzuela, Felipe Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Zamorano, Patricia Ines. Universidad del Salvador; ArgentinaFil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaWiley2021-01-15T13:48:31Z2021-01-15T13:48:31Z2021-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8603https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.137181865-1674https://doi.org/10.1111/tbed.13718Transboundary and emerging diseases 68 (2) : 587-597 (March 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNBIO-1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular.info:eu-repo/semantics/restrictedAccess2025-09-11T10:23:37Zoai:localhost:20.500.12123/8603instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:23:38.123INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1
title MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1
spellingShingle MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1
Kornuta, Claudia Alejandra
Liposomes (Organelles)
Liposomas (Organulos)
Inmunogenética
Vacuna
Herpesviridae
Immunogenetics
Vaccines
title_short MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1
title_full MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1
title_fullStr MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1
title_full_unstemmed MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1
title_sort MANα1-2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV-1
dc.creator.none.fl_str_mv Kornuta, Claudia Alejandra
Bidart, , Juan Esteban
Soria, Ivana
Gammella, Mariela
Quattrocchi, Valeria
Pappalardo, Juan Sebastián
Salmaso, Stefano
Torchilin, Vladimir P.
Cheuquepan Valenzuel, Felipe Andres
Hecker, Yanina
Moore, Dadin P.
Zamorano, Patricia Ines
Langellotti, Cecilia Ana
author Kornuta, Claudia Alejandra
author_facet Kornuta, Claudia Alejandra
Bidart, , Juan Esteban
Soria, Ivana
Gammella, Mariela
Quattrocchi, Valeria
Pappalardo, Juan Sebastián
Salmaso, Stefano
Torchilin, Vladimir P.
Cheuquepan Valenzuel, Felipe Andres
Hecker, Yanina
Moore, Dadin P.
Zamorano, Patricia Ines
Langellotti, Cecilia Ana
author_role author
author2 Bidart, , Juan Esteban
Soria, Ivana
Gammella, Mariela
Quattrocchi, Valeria
Pappalardo, Juan Sebastián
Salmaso, Stefano
Torchilin, Vladimir P.
Cheuquepan Valenzuel, Felipe Andres
Hecker, Yanina
Moore, Dadin P.
Zamorano, Patricia Ines
Langellotti, Cecilia Ana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Liposomes (Organelles)
Liposomas (Organulos)
Inmunogenética
Vacuna
Herpesviridae
Immunogenetics
Vaccines
topic Liposomes (Organelles)
Liposomas (Organulos)
Inmunogenética
Vacuna
Herpesviridae
Immunogenetics
Vaccines
dc.description.none.fl_txt_mv New technologies in the field of vaccinology arise as a necessity for the treatment and control of many diseases. Whole virus inactivated vaccines and modified live virus ones used against Bovine Herpesvirus‐1 (BoHV‐1) infection have several disadvantages. Previous works on DNA vaccines against BoHV‐1 have demonstrated the capability to induce humoral and cellular immune responses. Nevertheless, ‘naked’ DNA induces low immunogenic response. Thus, loading of antigen encoding DNA sequences in liposomal formulations targeting dendritic cell receptors could be a promising strategy to better activate these antigen‐presenting cells (APC). In this work, a DNA‐based vaccine encoding the truncated version of BoHV‐1 glycoprotein D (pCIgD) was evaluated alone and encapsulated in a liposomal formulation containing LPS and decorated with MANα1‐2MAN‐PEG‐DOPE (pCIgD‐Man‐L). The vaccinations were performed in mice and bovines. The results showed that the use of pCIgD‐Man‐L enhanced the immune response in both animal models. For humoral immunity, significant differences were achieved when total antibody titres and isotypes were assayed in sera. Regarding cellular immunity, a significant increase in the proliferative response against BoHV‐1 was detected in animals vaccinated with pCIgD‐Man‐L when compared to the response induced in animals vaccinated with pCIgD. In addition, upregulation of CD40 molecules on the surface of bovine dendritic cells (DCs) was observed when cells were stimulated and activated with the vaccine formulations. When viral challenge was performed, bovines vaccinated with MANα1‐2MAN‐PEG‐DOPE elicited better protection which was evidenced by a lower viral excretion. These results demonstrate that the dendritic cell targeting using MANα1‐2MAN decorated liposomes can boost the immunogenicity resulting in a long‐lasting immunity. Liposomes decorated with MANα1‐2MAN‐PEG‐DOPE were tested for the first time as a DNA vaccine nanovehicle in cattle as a preventive treatment against BoHV‐1. These results open new perspectives for the design of vaccines for the control of bovine rhinotracheitis.
Instituto de Virología
Fil: Kornuta, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Bidart, Juan Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Soria, Ivana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gammella, Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Quattrocchi, Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Pappalardo, Juan Sebastián. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Salmaso, Stefano. Universita degli Studi di Padova. Department of Pharmaceutical and Pharmacological Sciences; Italia
Fil: Torchilin, Vladimir P. Northeastern University. Center for Pharmaceutical Biotechnology and Nanomedicine; Estados Unidos
Fil: Cheuquepán Valenzuela, Felipe Andrés. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina.
Fil: Hecker, Yanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Moore, Dadin P. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS); Argentina
Fil: Zamorano, Patricia Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Langellotti, Cecilia Ana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Kornuta, Claudia Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Bidart, Juan Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Soria, Ivana. Consejo Nacional de Investigaciones Científicas y Tecnológicas (CONICET); Argentina
Fil: Gammella, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Quattrocchi, Valeria. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Cheuquepán Valenzuela, Felipe Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Cheuquepán Valenzuela, Felipe Andrés. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Zamorano, Patricia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Zamorano, Patricia Ines. Universidad del Salvador; Argentina
Fil: Hecker, Yanina. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
description New technologies in the field of vaccinology arise as a necessity for the treatment and control of many diseases. Whole virus inactivated vaccines and modified live virus ones used against Bovine Herpesvirus‐1 (BoHV‐1) infection have several disadvantages. Previous works on DNA vaccines against BoHV‐1 have demonstrated the capability to induce humoral and cellular immune responses. Nevertheless, ‘naked’ DNA induces low immunogenic response. Thus, loading of antigen encoding DNA sequences in liposomal formulations targeting dendritic cell receptors could be a promising strategy to better activate these antigen‐presenting cells (APC). In this work, a DNA‐based vaccine encoding the truncated version of BoHV‐1 glycoprotein D (pCIgD) was evaluated alone and encapsulated in a liposomal formulation containing LPS and decorated with MANα1‐2MAN‐PEG‐DOPE (pCIgD‐Man‐L). The vaccinations were performed in mice and bovines. The results showed that the use of pCIgD‐Man‐L enhanced the immune response in both animal models. For humoral immunity, significant differences were achieved when total antibody titres and isotypes were assayed in sera. Regarding cellular immunity, a significant increase in the proliferative response against BoHV‐1 was detected in animals vaccinated with pCIgD‐Man‐L when compared to the response induced in animals vaccinated with pCIgD. In addition, upregulation of CD40 molecules on the surface of bovine dendritic cells (DCs) was observed when cells were stimulated and activated with the vaccine formulations. When viral challenge was performed, bovines vaccinated with MANα1‐2MAN‐PEG‐DOPE elicited better protection which was evidenced by a lower viral excretion. These results demonstrate that the dendritic cell targeting using MANα1‐2MAN decorated liposomes can boost the immunogenicity resulting in a long‐lasting immunity. Liposomes decorated with MANα1‐2MAN‐PEG‐DOPE were tested for the first time as a DNA vaccine nanovehicle in cattle as a preventive treatment against BoHV‐1. These results open new perspectives for the design of vaccines for the control of bovine rhinotracheitis.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-15T13:48:31Z
2021-01-15T13:48:31Z
2021-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8603
https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13718
1865-1674
https://doi.org/10.1111/tbed.13718
url http://hdl.handle.net/20.500.12123/8603
https://onlinelibrary.wiley.com/doi/abs/10.1111/tbed.13718
https://doi.org/10.1111/tbed.13718
identifier_str_mv 1865-1674
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNBIO-1131032/AR./Desarrollo de herramientas biotecnológicas para la prevención y el control de enfermedades pecuarias: vacunas, diagnóstico y eIdemiología molecular.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Transboundary and emerging diseases 68 (2) : 587-597 (March 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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