Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels

Autores
Bustos, Dolores Angela; Lascano, Hernan Ramiro; Villasuso, Ana Laura; Machado, Estela; Senn, Eugenia; Cordoba, Alicia Beatriz; Taleisnik, Edith
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background and Aims: Experimental evidence in the literature suggests that O2•− produced in the elongation zone of roots and leaves by plasma membrane NADPH oxidase activity is required for growth. This study explores whether growth changes along the root tip induced by hyperosmotic treatments in Zea mays are associated with the distribution of apoplastic O2•−. Methods: Stress treatments were imposed using 150 mM NaCl or 300 mM sorbitol. Root elongation rates and the spatial distribution of growth rates in the root tip were measured. Apoplastic O2•− was determined using nitro blue tetrazolium, and H2O2 was determined using 2′, 7′-dichlorofluorescin. Key Results: In non-stressed plants, the distribution of accelerating growth and highest O2•− levels coincided along the root tip. Salt and osmotic stress of the same intensity had similar inhibitory effects on root elongation, but O2•− levels increased in sorbitol-treated roots and decreased in NaCl-treated roots. Conclusions: The lack of association between apoplastic O2•− levels and root growth inhibition under hyper-osmotic stress leads us to hypothesize that under those conditions the role of apoplastic O2•− may be to participate in signalling processes, that convey information on the nature of the substrate that the growing root is exploring.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Villasuso, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físicas, Químicas y Naturales. Química Biológica; Argentina
Fil: Machado, Estela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físicas, Químicas y Naturales. Química Biológica; Argentina
Fil: Senn, Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Cordoba, Alicia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fuente
Annals of Botany 102 (4) : 551–559 (October 2008)
Materia
Maíz
Zea Mays
Estrés Osmótico
Enraizamiento
Especies de Oxígeno Reactivo
Maize
Osmotic Stress
Rooting
Reactive Oxygen Species
Estres Salino
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6474
Acceder
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oai_identifier_str oai:localhost:20.500.12123/6474
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− LevelsBustos, Dolores AngelaLascano, Hernan RamiroVillasuso, Ana LauraMachado, EstelaSenn, EugeniaCordoba, Alicia BeatrizTaleisnik, EdithMaízZea MaysEstrés OsmóticoEnraizamientoEspecies de Oxígeno ReactivoMaizeOsmotic StressRootingReactive Oxygen SpeciesEstres SalinoBackground and Aims: Experimental evidence in the literature suggests that O2•− produced in the elongation zone of roots and leaves by plasma membrane NADPH oxidase activity is required for growth. This study explores whether growth changes along the root tip induced by hyperosmotic treatments in Zea mays are associated with the distribution of apoplastic O2•−. Methods: Stress treatments were imposed using 150 mM NaCl or 300 mM sorbitol. Root elongation rates and the spatial distribution of growth rates in the root tip were measured. Apoplastic O2•− was determined using nitro blue tetrazolium, and H2O2 was determined using 2′, 7′-dichlorofluorescin. Key Results: In non-stressed plants, the distribution of accelerating growth and highest O2•− levels coincided along the root tip. Salt and osmotic stress of the same intensity had similar inhibitory effects on root elongation, but O2•− levels increased in sorbitol-treated roots and decreased in NaCl-treated roots. Conclusions: The lack of association between apoplastic O2•− levels and root growth inhibition under hyper-osmotic stress leads us to hypothesize that under those conditions the role of apoplastic O2•− may be to participate in signalling processes, that convey information on the nature of the substrate that the growing root is exploring.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.Fil: Villasuso, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físicas, Químicas y Naturales. Química Biológica; ArgentinaFil: Machado, Estela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físicas, Químicas y Naturales. Química Biológica; ArgentinaFil: Senn, Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.Fil: Cordoba, Alicia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.Fil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.Oxford Academic Press2019-12-09T14:30:58Z2019-12-09T14:30:58Z2008-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6474https://academic.oup.com/aob/article/102/4/551/1664370305-73641095-8290https://doi.org/10.1093/aob/mcn141Annals of Botany 102 (4) : 551–559 (October 2008)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:50Zoai:localhost:20.500.12123/6474instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:50.859INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels
title Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels
spellingShingle Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels
Bustos, Dolores Angela
Maíz
Zea Mays
Estrés Osmótico
Enraizamiento
Especies de Oxígeno Reactivo
Maize
Osmotic Stress
Rooting
Reactive Oxygen Species
Estres Salino
title_short Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels
title_full Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels
title_fullStr Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels
title_full_unstemmed Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels
title_sort Reductions in Maize Root-tip Elongation by Salt and Osmotic Stress do not Correlate with Apoplastic O2•− Levels
dc.creator.none.fl_str_mv Bustos, Dolores Angela
Lascano, Hernan Ramiro
Villasuso, Ana Laura
Machado, Estela
Senn, Eugenia
Cordoba, Alicia Beatriz
Taleisnik, Edith
author Bustos, Dolores Angela
author_facet Bustos, Dolores Angela
Lascano, Hernan Ramiro
Villasuso, Ana Laura
Machado, Estela
Senn, Eugenia
Cordoba, Alicia Beatriz
Taleisnik, Edith
author_role author
author2 Lascano, Hernan Ramiro
Villasuso, Ana Laura
Machado, Estela
Senn, Eugenia
Cordoba, Alicia Beatriz
Taleisnik, Edith
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Maíz
Zea Mays
Estrés Osmótico
Enraizamiento
Especies de Oxígeno Reactivo
Maize
Osmotic Stress
Rooting
Reactive Oxygen Species
Estres Salino
topic Maíz
Zea Mays
Estrés Osmótico
Enraizamiento
Especies de Oxígeno Reactivo
Maize
Osmotic Stress
Rooting
Reactive Oxygen Species
Estres Salino
dc.description.none.fl_txt_mv Background and Aims: Experimental evidence in the literature suggests that O2•− produced in the elongation zone of roots and leaves by plasma membrane NADPH oxidase activity is required for growth. This study explores whether growth changes along the root tip induced by hyperosmotic treatments in Zea mays are associated with the distribution of apoplastic O2•−. Methods: Stress treatments were imposed using 150 mM NaCl or 300 mM sorbitol. Root elongation rates and the spatial distribution of growth rates in the root tip were measured. Apoplastic O2•− was determined using nitro blue tetrazolium, and H2O2 was determined using 2′, 7′-dichlorofluorescin. Key Results: In non-stressed plants, the distribution of accelerating growth and highest O2•− levels coincided along the root tip. Salt and osmotic stress of the same intensity had similar inhibitory effects on root elongation, but O2•− levels increased in sorbitol-treated roots and decreased in NaCl-treated roots. Conclusions: The lack of association between apoplastic O2•− levels and root growth inhibition under hyper-osmotic stress leads us to hypothesize that under those conditions the role of apoplastic O2•− may be to participate in signalling processes, that convey information on the nature of the substrate that the growing root is exploring.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Bustos, Dolores Angela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Villasuso, Ana Laura. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físicas, Químicas y Naturales. Química Biológica; Argentina
Fil: Machado, Estela. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físicas, Químicas y Naturales. Química Biológica; Argentina
Fil: Senn, Eugenia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Cordoba, Alicia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
Fil: Taleisnik, Edith. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina.
description Background and Aims: Experimental evidence in the literature suggests that O2•− produced in the elongation zone of roots and leaves by plasma membrane NADPH oxidase activity is required for growth. This study explores whether growth changes along the root tip induced by hyperosmotic treatments in Zea mays are associated with the distribution of apoplastic O2•−. Methods: Stress treatments were imposed using 150 mM NaCl or 300 mM sorbitol. Root elongation rates and the spatial distribution of growth rates in the root tip were measured. Apoplastic O2•− was determined using nitro blue tetrazolium, and H2O2 was determined using 2′, 7′-dichlorofluorescin. Key Results: In non-stressed plants, the distribution of accelerating growth and highest O2•− levels coincided along the root tip. Salt and osmotic stress of the same intensity had similar inhibitory effects on root elongation, but O2•− levels increased in sorbitol-treated roots and decreased in NaCl-treated roots. Conclusions: The lack of association between apoplastic O2•− levels and root growth inhibition under hyper-osmotic stress leads us to hypothesize that under those conditions the role of apoplastic O2•− may be to participate in signalling processes, that convey information on the nature of the substrate that the growing root is exploring.
publishDate 2008
dc.date.none.fl_str_mv 2008-10
2019-12-09T14:30:58Z
2019-12-09T14:30:58Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6474
https://academic.oup.com/aob/article/102/4/551/166437
0305-7364
1095-8290
https://doi.org/10.1093/aob/mcn141
url http://hdl.handle.net/20.500.12123/6474
https://academic.oup.com/aob/article/102/4/551/166437
https://doi.org/10.1093/aob/mcn141
identifier_str_mv 0305-7364
1095-8290
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford Academic Press
publisher.none.fl_str_mv Oxford Academic Press
dc.source.none.fl_str_mv Annals of Botany 102 (4) : 551–559 (October 2008)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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