Experimental inoculation of Neospora caninum in pregnant water buffalo

Autores
Konrad, José Luis; Moore, Dadin Prando; Crudeli, Gustavo Angel; Caspe, S. G.; Cano, D. B.; Leunda, M. R.; Lischinsky, L.; Regidor Cerrillo, J.; Odeón, A. C.; Ortega Mora, L. M.; Echaide, I.; Campero, C. M.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to characterize the pathogenesis of Neospora caninum in experimentally inoculated pregnant water buffalo (Bubalus bubalis). Twelve Mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to N. caninum by indirect fluorescent antibody test (IFAT) were involved. Ten females were intravenously inoculated with 108 tachyzoites of NC-1 strain at 70 (n = 3) or 90 (n = 7) days of pregnancy (dp). Two control animals were inoculated with placebo at 70 and 90 dp, respectively. Serum samples were obtained weekly following inoculation to the end of the experiment. Three animals inoculated at 70 dp were slaughtered at 28 days post inoculation (dpi), three animals inoculated at 90 dp were slaughtered at 28 dpi and the remaining four animals inoculated at 90 dp were slaughtered at 42 dpi. Fetal fluids from cavities and tissue samples were recovered for IFAT and histopathology, immunohistochemistry and PCR, respectively. Genomic DNA from fetal tissues was used for parasite DNA detection and microsatellite genotyping in order to confirm the NC-1 specific-infection. Dams developed specific antibodies one week after the inoculation and serological titers did not decrease significantly to the end of the experiment. No abortions were recorded during the experimental time; however, one fetus from a dam inoculated at 70 dp was not viable at necropsy. Specific antibodies were detected in only two fetuses from dams inoculated at 90 dp that were slaughtered at 42 dpi. No macroscopic changes in the placentas and organs of viable fetuses were observed. Nonsuppurative placentitis was a common microscopic observation in Neospora-inoculated specimens. Microscopic fetal lesions included nonsuppurative peribronchiolar interstitial pneumonia, epicarditis and myocarditis, interstitial nephritis, myositis and periportal hepatitis. Positive IHC results were obtained in two fetuses from dams inoculated at 70 dp and slaughtered at 28 dpi. N. caninum DNA was detected in placentas and fetuses from all inoculated animals. The pattern of amplified microsatellites from placental and fetal tissues resembled the NC-1 strain. Water buffaloes, like cattle, are susceptible to experimental inoculation with N. caninum at early pregnancy.
Fil: Konrad, José Luis. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Crudeli, Gustavo Angel. Universidad Nacional del Nordeste; Argentina
Fil: Caspe, S. G.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Cano, D. B.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Leunda, M. R.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Lischinsky, L.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Regidor Cerrillo, J.. Universidad Complutense de Madrid; España
Fil: Odeón, A. C.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Ortega Mora, L. M.. Universidad Complutense de Madrid; España
Fil: Echaide, I.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Campero, C. M.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Materia
FETAL PATHOLOGY
NEOSPORA CANINUM
WATER BUFFALO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/196380
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/196380
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Experimental inoculation of Neospora caninum in pregnant water buffaloKonrad, José LuisMoore, Dadin PrandoCrudeli, Gustavo AngelCaspe, S. G.Cano, D. B.Leunda, M. R.Lischinsky, L.Regidor Cerrillo, J.Odeón, A. C.Ortega Mora, L. M.Echaide, I.Campero, C. M.FETAL PATHOLOGYNEOSPORA CANINUMWATER BUFFALOhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to characterize the pathogenesis of Neospora caninum in experimentally inoculated pregnant water buffalo (Bubalus bubalis). Twelve Mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to N. caninum by indirect fluorescent antibody test (IFAT) were involved. Ten females were intravenously inoculated with 108 tachyzoites of NC-1 strain at 70 (n = 3) or 90 (n = 7) days of pregnancy (dp). Two control animals were inoculated with placebo at 70 and 90 dp, respectively. Serum samples were obtained weekly following inoculation to the end of the experiment. Three animals inoculated at 70 dp were slaughtered at 28 days post inoculation (dpi), three animals inoculated at 90 dp were slaughtered at 28 dpi and the remaining four animals inoculated at 90 dp were slaughtered at 42 dpi. Fetal fluids from cavities and tissue samples were recovered for IFAT and histopathology, immunohistochemistry and PCR, respectively. Genomic DNA from fetal tissues was used for parasite DNA detection and microsatellite genotyping in order to confirm the NC-1 specific-infection. Dams developed specific antibodies one week after the inoculation and serological titers did not decrease significantly to the end of the experiment. No abortions were recorded during the experimental time; however, one fetus from a dam inoculated at 70 dp was not viable at necropsy. Specific antibodies were detected in only two fetuses from dams inoculated at 90 dp that were slaughtered at 42 dpi. No macroscopic changes in the placentas and organs of viable fetuses were observed. Nonsuppurative placentitis was a common microscopic observation in Neospora-inoculated specimens. Microscopic fetal lesions included nonsuppurative peribronchiolar interstitial pneumonia, epicarditis and myocarditis, interstitial nephritis, myositis and periportal hepatitis. Positive IHC results were obtained in two fetuses from dams inoculated at 70 dp and slaughtered at 28 dpi. N. caninum DNA was detected in placentas and fetuses from all inoculated animals. The pattern of amplified microsatellites from placental and fetal tissues resembled the NC-1 strain. Water buffaloes, like cattle, are susceptible to experimental inoculation with N. caninum at early pregnancy.Fil: Konrad, José Luis. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; ArgentinaFil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Crudeli, Gustavo Angel. Universidad Nacional del Nordeste; ArgentinaFil: Caspe, S. G.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Cano, D. B.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Leunda, M. R.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Lischinsky, L.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Regidor Cerrillo, J.. Universidad Complutense de Madrid; EspañaFil: Odeón, A. C.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Ortega Mora, L. M.. Universidad Complutense de Madrid; EspañaFil: Echaide, I.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Campero, C. M.. Instituto Nacional de Tecnología Agropecuaria; ArgentinaElsevier Science2012-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/196380Konrad, José Luis; Moore, Dadin Prando; Crudeli, Gustavo Angel; Caspe, S. G.; Cano, D. B.; et al.; Experimental inoculation of Neospora caninum in pregnant water buffalo; Elsevier Science; Veterinary Parasitology; 187; 1-2; 1-2012; 72-780304-4017CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0304401711008685?via%3Dihubinfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2011.12.030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:17:14Zoai:ri.conicet.gov.ar:11336/196380instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:17:14.579CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Experimental inoculation of Neospora caninum in pregnant water buffalo
title Experimental inoculation of Neospora caninum in pregnant water buffalo
spellingShingle Experimental inoculation of Neospora caninum in pregnant water buffalo
Konrad, José Luis
FETAL PATHOLOGY
NEOSPORA CANINUM
WATER BUFFALO
title_short Experimental inoculation of Neospora caninum in pregnant water buffalo
title_full Experimental inoculation of Neospora caninum in pregnant water buffalo
title_fullStr Experimental inoculation of Neospora caninum in pregnant water buffalo
title_full_unstemmed Experimental inoculation of Neospora caninum in pregnant water buffalo
title_sort Experimental inoculation of Neospora caninum in pregnant water buffalo
dc.creator.none.fl_str_mv Konrad, José Luis
Moore, Dadin Prando
Crudeli, Gustavo Angel
Caspe, S. G.
Cano, D. B.
Leunda, M. R.
Lischinsky, L.
Regidor Cerrillo, J.
Odeón, A. C.
Ortega Mora, L. M.
Echaide, I.
Campero, C. M.
author Konrad, José Luis
author_facet Konrad, José Luis
Moore, Dadin Prando
Crudeli, Gustavo Angel
Caspe, S. G.
Cano, D. B.
Leunda, M. R.
Lischinsky, L.
Regidor Cerrillo, J.
Odeón, A. C.
Ortega Mora, L. M.
Echaide, I.
Campero, C. M.
author_role author
author2 Moore, Dadin Prando
Crudeli, Gustavo Angel
Caspe, S. G.
Cano, D. B.
Leunda, M. R.
Lischinsky, L.
Regidor Cerrillo, J.
Odeón, A. C.
Ortega Mora, L. M.
Echaide, I.
Campero, C. M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FETAL PATHOLOGY
NEOSPORA CANINUM
WATER BUFFALO
topic FETAL PATHOLOGY
NEOSPORA CANINUM
WATER BUFFALO
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to characterize the pathogenesis of Neospora caninum in experimentally inoculated pregnant water buffalo (Bubalus bubalis). Twelve Mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to N. caninum by indirect fluorescent antibody test (IFAT) were involved. Ten females were intravenously inoculated with 108 tachyzoites of NC-1 strain at 70 (n = 3) or 90 (n = 7) days of pregnancy (dp). Two control animals were inoculated with placebo at 70 and 90 dp, respectively. Serum samples were obtained weekly following inoculation to the end of the experiment. Three animals inoculated at 70 dp were slaughtered at 28 days post inoculation (dpi), three animals inoculated at 90 dp were slaughtered at 28 dpi and the remaining four animals inoculated at 90 dp were slaughtered at 42 dpi. Fetal fluids from cavities and tissue samples were recovered for IFAT and histopathology, immunohistochemistry and PCR, respectively. Genomic DNA from fetal tissues was used for parasite DNA detection and microsatellite genotyping in order to confirm the NC-1 specific-infection. Dams developed specific antibodies one week after the inoculation and serological titers did not decrease significantly to the end of the experiment. No abortions were recorded during the experimental time; however, one fetus from a dam inoculated at 70 dp was not viable at necropsy. Specific antibodies were detected in only two fetuses from dams inoculated at 90 dp that were slaughtered at 42 dpi. No macroscopic changes in the placentas and organs of viable fetuses were observed. Nonsuppurative placentitis was a common microscopic observation in Neospora-inoculated specimens. Microscopic fetal lesions included nonsuppurative peribronchiolar interstitial pneumonia, epicarditis and myocarditis, interstitial nephritis, myositis and periportal hepatitis. Positive IHC results were obtained in two fetuses from dams inoculated at 70 dp and slaughtered at 28 dpi. N. caninum DNA was detected in placentas and fetuses from all inoculated animals. The pattern of amplified microsatellites from placental and fetal tissues resembled the NC-1 strain. Water buffaloes, like cattle, are susceptible to experimental inoculation with N. caninum at early pregnancy.
Fil: Konrad, José Luis. Universidad Nacional del Nordeste; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
Fil: Moore, Dadin Prando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Crudeli, Gustavo Angel. Universidad Nacional del Nordeste; Argentina
Fil: Caspe, S. G.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Cano, D. B.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Leunda, M. R.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Lischinsky, L.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Regidor Cerrillo, J.. Universidad Complutense de Madrid; España
Fil: Odeón, A. C.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Ortega Mora, L. M.. Universidad Complutense de Madrid; España
Fil: Echaide, I.. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Campero, C. M.. Instituto Nacional de Tecnología Agropecuaria; Argentina
description The aim of this study was to characterize the pathogenesis of Neospora caninum in experimentally inoculated pregnant water buffalo (Bubalus bubalis). Twelve Mediterranean female water buffaloes ranging in age from 4 to 14 years old and seronegative to N. caninum by indirect fluorescent antibody test (IFAT) were involved. Ten females were intravenously inoculated with 108 tachyzoites of NC-1 strain at 70 (n = 3) or 90 (n = 7) days of pregnancy (dp). Two control animals were inoculated with placebo at 70 and 90 dp, respectively. Serum samples were obtained weekly following inoculation to the end of the experiment. Three animals inoculated at 70 dp were slaughtered at 28 days post inoculation (dpi), three animals inoculated at 90 dp were slaughtered at 28 dpi and the remaining four animals inoculated at 90 dp were slaughtered at 42 dpi. Fetal fluids from cavities and tissue samples were recovered for IFAT and histopathology, immunohistochemistry and PCR, respectively. Genomic DNA from fetal tissues was used for parasite DNA detection and microsatellite genotyping in order to confirm the NC-1 specific-infection. Dams developed specific antibodies one week after the inoculation and serological titers did not decrease significantly to the end of the experiment. No abortions were recorded during the experimental time; however, one fetus from a dam inoculated at 70 dp was not viable at necropsy. Specific antibodies were detected in only two fetuses from dams inoculated at 90 dp that were slaughtered at 42 dpi. No macroscopic changes in the placentas and organs of viable fetuses were observed. Nonsuppurative placentitis was a common microscopic observation in Neospora-inoculated specimens. Microscopic fetal lesions included nonsuppurative peribronchiolar interstitial pneumonia, epicarditis and myocarditis, interstitial nephritis, myositis and periportal hepatitis. Positive IHC results were obtained in two fetuses from dams inoculated at 70 dp and slaughtered at 28 dpi. N. caninum DNA was detected in placentas and fetuses from all inoculated animals. The pattern of amplified microsatellites from placental and fetal tissues resembled the NC-1 strain. Water buffaloes, like cattle, are susceptible to experimental inoculation with N. caninum at early pregnancy.
publishDate 2012
dc.date.none.fl_str_mv 2012-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/196380
Konrad, José Luis; Moore, Dadin Prando; Crudeli, Gustavo Angel; Caspe, S. G.; Cano, D. B.; et al.; Experimental inoculation of Neospora caninum in pregnant water buffalo; Elsevier Science; Veterinary Parasitology; 187; 1-2; 1-2012; 72-78
0304-4017
CONICET Digital
CONICET
url http://hdl.handle.net/11336/196380
identifier_str_mv Konrad, José Luis; Moore, Dadin Prando; Crudeli, Gustavo Angel; Caspe, S. G.; Cano, D. B.; et al.; Experimental inoculation of Neospora caninum in pregnant water buffalo; Elsevier Science; Veterinary Parasitology; 187; 1-2; 1-2012; 72-78
0304-4017
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0304401711008685?via%3Dihub
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2011.12.030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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