Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum

Autores
Canton, German Jose; Katzer, Frank; Maley, Stephen W.; Bartley, Paul M.; Benavides-Silván, Julio; Palarea-Albaladejo, Javier; Pang, Yvonne; Smith, Sionagh; Rocchi, Mara; Buxton, David; Innes, Elisabeth A.; Chianini, Francesca
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neospora caninum is recognized as a major cause of reproductive losses worldwide but its pathogenesis is not completely understood. Immune mediated placental pathology has been reported as being responsible for compromising pregnancy probably due to the adverse effects of exacerbated Th1 type response at the maternal–foetal interface. Different clinical outcomes are known to occur following experimental infections of cattle at different stages of gestation, with foetal death being the most common finding during early gestation, and the birth of live congenitally infected calves following infection later in gestation. The aim of the current study was to characterize the cytokine expression in the placenta of cattle experimentally challenged with tachyzoites of the Nc-1 strain during early, mid and late gestation. Moderate to severe infiltration of IL-12, IFN-γ and TNF-α expressing cells was observed in the placentas collected at early gestation and this infiltration was more pronounced in the samples collected from challenged dams carrying non-viable foetuses, compared with the mothers carrying viable foetuses. In contrast, the infiltration of Th1 cytokine expressing-cells was mild following N. caninum infection in mid gestation and scarce during infection in late gestation. Scarce expression of IL-4 was observed in the placentas from N. caninum-challenged and negative control animals throughout gestation. The milder Th1 immune response observed during later stages of gestation following Nc-1 infection could partially explain the less severe clinical outcome when compared to early pregnancy.
EEA Balcarce
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España
Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido
Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido
Fil: Smith, Sionagh. University of Edinburgh. Royal (Dick) School of Veterinary Studies and Roslin Institute; Reino Unido
Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido
Fil: Buxton, David. Moredun Research Institute; Reino Unido
Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fuente
Veterinary Immunology and Immunopathology 161 (1–2) : 77-89 (September 2014)
Materia
Ganado Bovino
Neospora caninum
Gestación
Enfermedades de los Animales
Citoquinas
Placenta
Cattle
Pregnancy
Animal Diseases
Cytokines
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4371
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4371
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninumCanton, German JoseKatzer, FrankMaley, Stephen W.Bartley, Paul M.Benavides-Silván, JulioPalarea-Albaladejo, JavierPang, YvonneSmith, SionaghRocchi, MaraBuxton, DavidInnes, Elisabeth A.Chianini, FrancescaGanado BovinoNeospora caninumGestaciónEnfermedades de los AnimalesCitoquinasPlacentaCattlePregnancyAnimal DiseasesCytokinesNeospora caninum is recognized as a major cause of reproductive losses worldwide but its pathogenesis is not completely understood. Immune mediated placental pathology has been reported as being responsible for compromising pregnancy probably due to the adverse effects of exacerbated Th1 type response at the maternal–foetal interface. Different clinical outcomes are known to occur following experimental infections of cattle at different stages of gestation, with foetal death being the most common finding during early gestation, and the birth of live congenitally infected calves following infection later in gestation. The aim of the current study was to characterize the cytokine expression in the placenta of cattle experimentally challenged with tachyzoites of the Nc-1 strain during early, mid and late gestation. Moderate to severe infiltration of IL-12, IFN-γ and TNF-α expressing cells was observed in the placentas collected at early gestation and this infiltration was more pronounced in the samples collected from challenged dams carrying non-viable foetuses, compared with the mothers carrying viable foetuses. In contrast, the infiltration of Th1 cytokine expressing-cells was mild following N. caninum infection in mid gestation and scarce during infection in late gestation. Scarce expression of IL-4 was observed in the placentas from N. caninum-challenged and negative control animals throughout gestation. The milder Th1 immune response observed during later stages of gestation following Nc-1 infection could partially explain the less severe clinical outcome when compared to early pregnancy.EEA BalcarceFil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Katzer, Frank. Moredun Research Institute; Reino UnidoFil: Maley, Stephen W. Moredun Research Institute; Reino UnidoFil: Bartley, Paul M. Moredun Research Institute; Reino UnidoFil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; EspañaFil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino UnidoFil: Pang, Yvonne. Moredun Research Institute; Reino UnidoFil: Smith, Sionagh. University of Edinburgh. Royal (Dick) School of Veterinary Studies and Roslin Institute; Reino UnidoFil: Rocchi, Mara. Moredun Research Institute; Reino UnidoFil: Buxton, David. Moredun Research Institute; Reino UnidoFil: Innes, Elisabeth A. Moredun Research Institute; Reino UnidoFil: Chianini, Francesca. Moredun Research Institute; Reino UnidoElsevier2019-02-01T11:54:30Z2019-02-01T11:54:30Z2014-09-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0165242714001676http://hdl.handle.net/20.500.12123/43710165-24271873-2534https://doi.org/10.1016/j.vetimm.2014.07.004Veterinary Immunology and Immunopathology 161 (1–2) : 77-89 (September 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:26Zoai:localhost:20.500.12123/4371instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:26.362INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum
title Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum
spellingShingle Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum
Canton, German Jose
Ganado Bovino
Neospora caninum
Gestación
Enfermedades de los Animales
Citoquinas
Placenta
Cattle
Pregnancy
Animal Diseases
Cytokines
title_short Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum
title_full Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum
title_fullStr Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum
title_full_unstemmed Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum
title_sort Cytokine expression in the placenta of pregnant cattle after inoculation with Neospora caninum
dc.creator.none.fl_str_mv Canton, German Jose
Katzer, Frank
Maley, Stephen W.
Bartley, Paul M.
Benavides-Silván, Julio
Palarea-Albaladejo, Javier
Pang, Yvonne
Smith, Sionagh
Rocchi, Mara
Buxton, David
Innes, Elisabeth A.
Chianini, Francesca
author Canton, German Jose
author_facet Canton, German Jose
Katzer, Frank
Maley, Stephen W.
Bartley, Paul M.
Benavides-Silván, Julio
Palarea-Albaladejo, Javier
Pang, Yvonne
Smith, Sionagh
Rocchi, Mara
Buxton, David
Innes, Elisabeth A.
Chianini, Francesca
author_role author
author2 Katzer, Frank
Maley, Stephen W.
Bartley, Paul M.
Benavides-Silván, Julio
Palarea-Albaladejo, Javier
Pang, Yvonne
Smith, Sionagh
Rocchi, Mara
Buxton, David
Innes, Elisabeth A.
Chianini, Francesca
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Neospora caninum
Gestación
Enfermedades de los Animales
Citoquinas
Placenta
Cattle
Pregnancy
Animal Diseases
Cytokines
topic Ganado Bovino
Neospora caninum
Gestación
Enfermedades de los Animales
Citoquinas
Placenta
Cattle
Pregnancy
Animal Diseases
Cytokines
dc.description.none.fl_txt_mv Neospora caninum is recognized as a major cause of reproductive losses worldwide but its pathogenesis is not completely understood. Immune mediated placental pathology has been reported as being responsible for compromising pregnancy probably due to the adverse effects of exacerbated Th1 type response at the maternal–foetal interface. Different clinical outcomes are known to occur following experimental infections of cattle at different stages of gestation, with foetal death being the most common finding during early gestation, and the birth of live congenitally infected calves following infection later in gestation. The aim of the current study was to characterize the cytokine expression in the placenta of cattle experimentally challenged with tachyzoites of the Nc-1 strain during early, mid and late gestation. Moderate to severe infiltration of IL-12, IFN-γ and TNF-α expressing cells was observed in the placentas collected at early gestation and this infiltration was more pronounced in the samples collected from challenged dams carrying non-viable foetuses, compared with the mothers carrying viable foetuses. In contrast, the infiltration of Th1 cytokine expressing-cells was mild following N. caninum infection in mid gestation and scarce during infection in late gestation. Scarce expression of IL-4 was observed in the placentas from N. caninum-challenged and negative control animals throughout gestation. The milder Th1 immune response observed during later stages of gestation following Nc-1 infection could partially explain the less severe clinical outcome when compared to early pregnancy.
EEA Balcarce
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España
Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido
Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido
Fil: Smith, Sionagh. University of Edinburgh. Royal (Dick) School of Veterinary Studies and Roslin Institute; Reino Unido
Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido
Fil: Buxton, David. Moredun Research Institute; Reino Unido
Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
description Neospora caninum is recognized as a major cause of reproductive losses worldwide but its pathogenesis is not completely understood. Immune mediated placental pathology has been reported as being responsible for compromising pregnancy probably due to the adverse effects of exacerbated Th1 type response at the maternal–foetal interface. Different clinical outcomes are known to occur following experimental infections of cattle at different stages of gestation, with foetal death being the most common finding during early gestation, and the birth of live congenitally infected calves following infection later in gestation. The aim of the current study was to characterize the cytokine expression in the placenta of cattle experimentally challenged with tachyzoites of the Nc-1 strain during early, mid and late gestation. Moderate to severe infiltration of IL-12, IFN-γ and TNF-α expressing cells was observed in the placentas collected at early gestation and this infiltration was more pronounced in the samples collected from challenged dams carrying non-viable foetuses, compared with the mothers carrying viable foetuses. In contrast, the infiltration of Th1 cytokine expressing-cells was mild following N. caninum infection in mid gestation and scarce during infection in late gestation. Scarce expression of IL-4 was observed in the placentas from N. caninum-challenged and negative control animals throughout gestation. The milder Th1 immune response observed during later stages of gestation following Nc-1 infection could partially explain the less severe clinical outcome when compared to early pregnancy.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-15
2019-02-01T11:54:30Z
2019-02-01T11:54:30Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0165242714001676
http://hdl.handle.net/20.500.12123/4371
0165-2427
1873-2534
https://doi.org/10.1016/j.vetimm.2014.07.004
url https://www.sciencedirect.com/science/article/pii/S0165242714001676
http://hdl.handle.net/20.500.12123/4371
https://doi.org/10.1016/j.vetimm.2014.07.004
identifier_str_mv 0165-2427
1873-2534
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Immunology and Immunopathology 161 (1–2) : 77-89 (September 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846143510645833728
score 12.712165

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