Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation

Autores
Canton, German Jose; Katzer, Frank; Benavides-Silván, Julio; Maley, Stephen W.; Palarea-Albaladejo, Javier; Pang, Yvonne; Smith, Sionagh; Bartley, Paul M.; Rocchi, Mara; Innes, Elisabeth A.; Chianini, Francesca
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Despite Neospora caninum being a major cause of bovine abortion worldwide, its pathogenesis is not completely understood. Neospora infection stimulates host cell-mediated immune responses, which may be responsible for the placental damage leading to abortion. The aim of the current study was to characterize the placental immune response following an experimental inoculation of pregnant cattle with N. caninum tachyzoites at day 210 of gestation. Cows were culled at 14, 28, 42 and 56 days post inoculation (dpi). Placentomes were examined by immunohistochemistry using antibodies against macrophages, T-cell subsets (CD4, CD8 and γδ), NK cells and B cells. Macrophages were detected mainly at 14 days post inoculation. Inflammation was generally mild and mainly characterized by CD3+, CD4+ and γδ T-cells; whereas CD8+ and NK cells were less numerous. The immune cell repertoire observed in this study was similar to those seen in pregnant cattle challenged with N. caninum at early gestation. However, cellular infiltrates were less severe than those seen during first trimester Neospora infections. This may explain the milder clinical outcome observed when animals are infected late in gestation.
EEA Balcarce
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España
Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido
Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido
Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido
Fil: Smith, Sionagh. University of Edinburgh. Royal (Dick) School of Veterinary Studies and Roslin Institute; Reino Unido
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido
Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fuente
Veterinary Research 44 : 60 (2013)
Materia
Neospora caninum
Ganado Bovino
Placenta
Fenotipos
Gestación
Inoculación
Cattle
Phenotypes
Pregnancy
Inoculation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3948
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3948
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spelling Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestationCanton, German JoseKatzer, FrankBenavides-Silván, JulioMaley, Stephen W.Palarea-Albaladejo, JavierPang, YvonneSmith, SionaghBartley, Paul M.Rocchi, MaraInnes, Elisabeth A.Chianini, FrancescaNeospora caninumGanado BovinoPlacentaFenotiposGestaciónInoculaciónCattlePhenotypesPregnancyInoculationDespite Neospora caninum being a major cause of bovine abortion worldwide, its pathogenesis is not completely understood. Neospora infection stimulates host cell-mediated immune responses, which may be responsible for the placental damage leading to abortion. The aim of the current study was to characterize the placental immune response following an experimental inoculation of pregnant cattle with N. caninum tachyzoites at day 210 of gestation. Cows were culled at 14, 28, 42 and 56 days post inoculation (dpi). Placentomes were examined by immunohistochemistry using antibodies against macrophages, T-cell subsets (CD4, CD8 and γδ), NK cells and B cells. Macrophages were detected mainly at 14 days post inoculation. Inflammation was generally mild and mainly characterized by CD3+, CD4+ and γδ T-cells; whereas CD8+ and NK cells were less numerous. The immune cell repertoire observed in this study was similar to those seen in pregnant cattle challenged with N. caninum at early gestation. However, cellular infiltrates were less severe than those seen during first trimester Neospora infections. This may explain the milder clinical outcome observed when animals are infected late in gestation.EEA BalcarceFil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Katzer, Frank. Moredun Research Institute; Reino UnidoFil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; EspañaFil: Maley, Stephen W. Moredun Research Institute; Reino UnidoFil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino UnidoFil: Pang, Yvonne. Moredun Research Institute; Reino UnidoFil: Smith, Sionagh. University of Edinburgh. Royal (Dick) School of Veterinary Studies and Roslin Institute; Reino UnidoFil: Bartley, Paul M. Moredun Research Institute; Reino UnidoFil: Rocchi, Mara. Moredun Research Institute; Reino UnidoFil: Innes, Elisabeth A. Moredun Research Institute; Reino UnidoFil: Chianini, Francesca. Moredun Research Institute; Reino UnidoBMC2018-11-22T14:44:14Z2018-11-22T14:44:14Z2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-60http://hdl.handle.net/20.500.12123/39480928-42491297-9716https://doi.org/10.1186/1297-9716-44-60Veterinary Research 44 : 60 (2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:30Zoai:localhost:20.500.12123/3948instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:30.876INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation
title Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation
spellingShingle Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation
Canton, German Jose
Neospora caninum
Ganado Bovino
Placenta
Fenotipos
Gestación
Inoculación
Cattle
Phenotypes
Pregnancy
Inoculation
title_short Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation
title_full Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation
title_fullStr Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation
title_full_unstemmed Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation
title_sort Phenotypic characterisation of the cellular immune infiltrate in placentas of cattle following experimental inoculation with Neospora caninum in late gestation
dc.creator.none.fl_str_mv Canton, German Jose
Katzer, Frank
Benavides-Silván, Julio
Maley, Stephen W.
Palarea-Albaladejo, Javier
Pang, Yvonne
Smith, Sionagh
Bartley, Paul M.
Rocchi, Mara
Innes, Elisabeth A.
Chianini, Francesca
author Canton, German Jose
author_facet Canton, German Jose
Katzer, Frank
Benavides-Silván, Julio
Maley, Stephen W.
Palarea-Albaladejo, Javier
Pang, Yvonne
Smith, Sionagh
Bartley, Paul M.
Rocchi, Mara
Innes, Elisabeth A.
Chianini, Francesca
author_role author
author2 Katzer, Frank
Benavides-Silván, Julio
Maley, Stephen W.
Palarea-Albaladejo, Javier
Pang, Yvonne
Smith, Sionagh
Bartley, Paul M.
Rocchi, Mara
Innes, Elisabeth A.
Chianini, Francesca
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Neospora caninum
Ganado Bovino
Placenta
Fenotipos
Gestación
Inoculación
Cattle
Phenotypes
Pregnancy
Inoculation
topic Neospora caninum
Ganado Bovino
Placenta
Fenotipos
Gestación
Inoculación
Cattle
Phenotypes
Pregnancy
Inoculation
dc.description.none.fl_txt_mv Despite Neospora caninum being a major cause of bovine abortion worldwide, its pathogenesis is not completely understood. Neospora infection stimulates host cell-mediated immune responses, which may be responsible for the placental damage leading to abortion. The aim of the current study was to characterize the placental immune response following an experimental inoculation of pregnant cattle with N. caninum tachyzoites at day 210 of gestation. Cows were culled at 14, 28, 42 and 56 days post inoculation (dpi). Placentomes were examined by immunohistochemistry using antibodies against macrophages, T-cell subsets (CD4, CD8 and γδ), NK cells and B cells. Macrophages were detected mainly at 14 days post inoculation. Inflammation was generally mild and mainly characterized by CD3+, CD4+ and γδ T-cells; whereas CD8+ and NK cells were less numerous. The immune cell repertoire observed in this study was similar to those seen in pregnant cattle challenged with N. caninum at early gestation. However, cellular infiltrates were less severe than those seen during first trimester Neospora infections. This may explain the milder clinical outcome observed when animals are infected late in gestation.
EEA Balcarce
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España
Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido
Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido
Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido
Fil: Smith, Sionagh. University of Edinburgh. Royal (Dick) School of Veterinary Studies and Roslin Institute; Reino Unido
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido
Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
description Despite Neospora caninum being a major cause of bovine abortion worldwide, its pathogenesis is not completely understood. Neospora infection stimulates host cell-mediated immune responses, which may be responsible for the placental damage leading to abortion. The aim of the current study was to characterize the placental immune response following an experimental inoculation of pregnant cattle with N. caninum tachyzoites at day 210 of gestation. Cows were culled at 14, 28, 42 and 56 days post inoculation (dpi). Placentomes were examined by immunohistochemistry using antibodies against macrophages, T-cell subsets (CD4, CD8 and γδ), NK cells and B cells. Macrophages were detected mainly at 14 days post inoculation. Inflammation was generally mild and mainly characterized by CD3+, CD4+ and γδ T-cells; whereas CD8+ and NK cells were less numerous. The immune cell repertoire observed in this study was similar to those seen in pregnant cattle challenged with N. caninum at early gestation. However, cellular infiltrates were less severe than those seen during first trimester Neospora infections. This may explain the milder clinical outcome observed when animals are infected late in gestation.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
2018-11-22T14:44:14Z
2018-11-22T14:44:14Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-60
http://hdl.handle.net/20.500.12123/3948
0928-4249
1297-9716
https://doi.org/10.1186/1297-9716-44-60
url https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-44-60
http://hdl.handle.net/20.500.12123/3948
https://doi.org/10.1186/1297-9716-44-60
identifier_str_mv 0928-4249
1297-9716
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv Veterinary Research 44 : 60 (2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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