Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus

Autores
Pujato, Silvina A.; Galliani, Valentina; Irazoqui, Jose Matias; Amadio, Ariel; Quiberoni, Andrea; Mercanti, Diego
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections.
EEA Rafaela
Fil: Pujato, Silvina A. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Pujato, Silvina A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina
Fil: Galliani, Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Galliani, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina
Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Quiberoni, Andrea. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina
Fil: Mercanti, DiegoUniversidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Mercanti, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina
Fuente
International Dairy Journal 118 : 105027 (July 2021)
Materia
Bacterias Acidolácticas
Lactobacillus
Lactobacillus casei
Genética
Lactic Acid Bacteria
Genetics
Lacticaseibacillus
CRISPR systems
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9014

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spelling Analysis of CRISPR systems of types II-A, I-E and I-C in strains of LacticaseibacillusPujato, Silvina A.Galliani, ValentinaIrazoqui, Jose MatiasAmadio, ArielQuiberoni, AndreaMercanti, DiegoBacterias AcidolácticasLactobacillusLactobacillus caseiGenéticaLactic Acid BacteriaGeneticsLacticaseibacillusCRISPR systemsA total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections.EEA RafaelaFil: Pujato, Silvina A. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Pujato, Silvina A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; ArgentinaFil: Galliani, Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Galliani, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; ArgentinaFil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Quiberoni, Andrea. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Quiberoni, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; ArgentinaFil: Mercanti, DiegoUniversidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Mercanti, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; ArgentinaElsevier2021-03-31T16:33:53Z2021-03-31T16:33:53Z2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9014https://www.sciencedirect.com/science/article/abs/pii/S09586946210005580958-6946https://doi.org/10.1016/j.idairyj.2021.105027International Dairy Journal 118 : 105027 (July 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:10Zoai:localhost:20.500.12123/9014instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:11.001INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
title Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
spellingShingle Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
Pujato, Silvina A.
Bacterias Acidolácticas
Lactobacillus
Lactobacillus casei
Genética
Lactic Acid Bacteria
Genetics
Lacticaseibacillus
CRISPR systems
title_short Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
title_full Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
title_fullStr Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
title_full_unstemmed Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
title_sort Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
dc.creator.none.fl_str_mv Pujato, Silvina A.
Galliani, Valentina
Irazoqui, Jose Matias
Amadio, Ariel
Quiberoni, Andrea
Mercanti, Diego
author Pujato, Silvina A.
author_facet Pujato, Silvina A.
Galliani, Valentina
Irazoqui, Jose Matias
Amadio, Ariel
Quiberoni, Andrea
Mercanti, Diego
author_role author
author2 Galliani, Valentina
Irazoqui, Jose Matias
Amadio, Ariel
Quiberoni, Andrea
Mercanti, Diego
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bacterias Acidolácticas
Lactobacillus
Lactobacillus casei
Genética
Lactic Acid Bacteria
Genetics
Lacticaseibacillus
CRISPR systems
topic Bacterias Acidolácticas
Lactobacillus
Lactobacillus casei
Genética
Lactic Acid Bacteria
Genetics
Lacticaseibacillus
CRISPR systems
dc.description.none.fl_txt_mv A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections.
EEA Rafaela
Fil: Pujato, Silvina A. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Pujato, Silvina A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina
Fil: Galliani, Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Galliani, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina
Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Quiberoni, Andrea. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina
Fil: Mercanti, DiegoUniversidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Mercanti, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina
description A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections.
publishDate 2021
dc.date.none.fl_str_mv 2021-03-31T16:33:53Z
2021-03-31T16:33:53Z
2021-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9014
https://www.sciencedirect.com/science/article/abs/pii/S0958694621000558
0958-6946
https://doi.org/10.1016/j.idairyj.2021.105027
url http://hdl.handle.net/20.500.12123/9014
https://www.sciencedirect.com/science/article/abs/pii/S0958694621000558
https://doi.org/10.1016/j.idairyj.2021.105027
identifier_str_mv 0958-6946
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv International Dairy Journal 118 : 105027 (July 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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