Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus
- Autores
- Pujato, Silvina A.; Galliani, Valentina; Irazoqui, Jose Matias; Amadio, Ariel; Quiberoni, Andrea; Mercanti, Diego
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections.
EEA Rafaela
Fil: Pujato, Silvina A. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Pujato, Silvina A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina
Fil: Galliani, Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Galliani, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina
Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Quiberoni, Andrea. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Quiberoni, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina
Fil: Mercanti, DiegoUniversidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina
Fil: Mercanti, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina - Fuente
- International Dairy Journal 118 : 105027 (July 2021)
- Materia
-
Bacterias Acidolácticas
Lactobacillus
Lactobacillus casei
Genética
Lactic Acid Bacteria
Genetics
Lacticaseibacillus
CRISPR systems - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/9014
Ver los metadatos del registro completo
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Analysis of CRISPR systems of types II-A, I-E and I-C in strains of LacticaseibacillusPujato, Silvina A.Galliani, ValentinaIrazoqui, Jose MatiasAmadio, ArielQuiberoni, AndreaMercanti, DiegoBacterias AcidolácticasLactobacillusLactobacillus caseiGenéticaLactic Acid BacteriaGeneticsLacticaseibacillusCRISPR systemsA total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections.EEA RafaelaFil: Pujato, Silvina A. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Pujato, Silvina A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; ArgentinaFil: Galliani, Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Galliani, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; ArgentinaFil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Quiberoni, Andrea. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Quiberoni, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; ArgentinaFil: Mercanti, DiegoUniversidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; ArgentinaFil: Mercanti, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; ArgentinaElsevier2021-03-31T16:33:53Z2021-03-31T16:33:53Z2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9014https://www.sciencedirect.com/science/article/abs/pii/S09586946210005580958-6946https://doi.org/10.1016/j.idairyj.2021.105027International Dairy Journal 118 : 105027 (July 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:10Zoai:localhost:20.500.12123/9014instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:11.001INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
title |
Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
spellingShingle |
Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus Pujato, Silvina A. Bacterias Acidolácticas Lactobacillus Lactobacillus casei Genética Lactic Acid Bacteria Genetics Lacticaseibacillus CRISPR systems |
title_short |
Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
title_full |
Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
title_fullStr |
Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
title_full_unstemmed |
Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
title_sort |
Analysis of CRISPR systems of types II-A, I-E and I-C in strains of Lacticaseibacillus |
dc.creator.none.fl_str_mv |
Pujato, Silvina A. Galliani, Valentina Irazoqui, Jose Matias Amadio, Ariel Quiberoni, Andrea Mercanti, Diego |
author |
Pujato, Silvina A. |
author_facet |
Pujato, Silvina A. Galliani, Valentina Irazoqui, Jose Matias Amadio, Ariel Quiberoni, Andrea Mercanti, Diego |
author_role |
author |
author2 |
Galliani, Valentina Irazoqui, Jose Matias Amadio, Ariel Quiberoni, Andrea Mercanti, Diego |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Bacterias Acidolácticas Lactobacillus Lactobacillus casei Genética Lactic Acid Bacteria Genetics Lacticaseibacillus CRISPR systems |
topic |
Bacterias Acidolácticas Lactobacillus Lactobacillus casei Genética Lactic Acid Bacteria Genetics Lacticaseibacillus CRISPR systems |
dc.description.none.fl_txt_mv |
A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections. EEA Rafaela Fil: Pujato, Silvina A. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Pujato, Silvina A. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina Fil: Galliani, Valentina. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Galliani, Valentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Amadio, Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Fil: Quiberoni, Andrea. Universidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Quiberoni, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina Fil: Mercanti, DiegoUniversidad Nacional del Litoral. Facultad de Ingeniería Química. Instituto de Lactología Industrial; Argentina Fil: Mercanti, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Lactología Industrial; Argentina |
description |
A total of 49 strains of Lacticaseibacillus were screened for the presence of CRISPR-Cas systems. Type II-A CRISPR-Cas loci were sequenced for 8 Lacticaseibacillus paracasei and 3 Lacticaseibacillus rhamnosus strains. They contained 21 to 49 spacers, a few of them with similarity to plasmids. Cas9 identity was well correlated with spacer content of the strains. Only strain L. paracasei 85 contained a type I-E CRISPR locus, uncommon in this species, with highly conserved Cas proteins and 79 spacers not previously reported, a high percentage of them (23%) matching L. paracasei phage genomes. PAM sequences were 5′-NGAA-3´ (type II-A) and 5′-aAA-3´ (type I-E). Protospacer selection in type I-E was highly biased towards genes involved in lysis and morphogenesis. Our results improve the knowledge about distribution and diversity of CRISPR-Cas systems in Lacticaseibacillus strains of industrial interest, not profoundly studied despite the economic impact of phage infections. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03-31T16:33:53Z 2021-03-31T16:33:53Z 2021-07 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/9014 https://www.sciencedirect.com/science/article/abs/pii/S0958694621000558 0958-6946 https://doi.org/10.1016/j.idairyj.2021.105027 |
url |
http://hdl.handle.net/20.500.12123/9014 https://www.sciencedirect.com/science/article/abs/pii/S0958694621000558 https://doi.org/10.1016/j.idairyj.2021.105027 |
identifier_str_mv |
0958-6946 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
International Dairy Journal 118 : 105027 (July 2021) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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12.559606 |