Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization
- Autores
- De Gregorio, Priscilla Romina; Lieske, Isadora; Nied, Cathy; Signori, Gabriela; Kuhn, Daniel; Schmitz, Caroline; Martins, Vera Lúcia Milani; Bustamante Filho, Ivan Cunha; Irazoqui, Jose Matias; Souza, Claucia Fernanda Volken de
- Año de publicación
- 2025
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- This study aimed to identify promising probiotic candidates among 25 autochthonous lactic acid bacteria (LAB) isolates from dairy products sourced from southeastern Brazil. In vitro assays were carried out to elucidate their phenotypic profiles, focusing on key probiotic attributes (antimicrobial, antioxidant and β-galactosidase activities, resistance to simulated gastrointestinal conditions, antibiotic susceptibility, and adhesion capacity). Principal Component Analysis was subsequently employed to rank the most promising strains. Among the isolates, four Lacticaseibacillus rhamnosus strains (6QLR5, GTM7, CLM11, and JLR3) demonstrated promising antimicrobial activity, adhesion capacity, tolerance to gastrointestinal conditions, antioxidant capacity, and β-galactosidase activity. Of these, strains 6QLR5 and GTM7 displayed the most robust phenotypic probiotic profiles and were selected for in silico genomic analysis. Functional annotation revealed genes associated with key probiotic traits, including redox systems related to antioxidant activity, adhesion-associated proteins, and mechanisms of resistance and adaptation to the harsh conditions of the intestinal tract. Genomic safety assessment, encompassing antimicrobial resistance genes, mobile genetic elements, virulence factors, and genome-stability features, indicated that strains 6QLR5 and GTM7 are safe probiotic candidates, as they harbored no genes linked to antibiotic resistance, pathogenicity, or virulence, and lacked hazardous plasmids or prophages. Overall, the characterization of autochthonous LAB strains based on integrative in vitro and in silico approaches proved effective for selecting probiotic candidates.
EEA Rafaela
Fil: De Gregorio, Priscilla Romina. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil
Fil: De Gregorio, Priscilla Romina. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil
Fil: De Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Referencia para Lactobacilos (CERELA); Argentina
Fil: Lieske, Isadora. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil
Fil: Lieske, Isadora. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil
Fil: Nied, Cathy. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil
Fil: Signori, Gabriela. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil
Fil: Kuhn, Daniel. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil
Fil: Schmitz, Caroline. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil
Fil: Martins, Vera Lúcia Milani. Federal Institute of Education, Science and Technology of Rio Grande Do Sul; Brasil
Fil: Bustamante Filho, Ivan Cunha. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil
Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina
Fil: Souza, Claucia Fernanda Volken de. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil
Fil: Souza, Claucia Fernanda Volken de. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil
In Vitro Experimentation - Fuente
- Food Bioscience 75 : 108066. (January 2026)
- Materia
-
Lactic Acid Bacteria
Probiotics
Genomics
Milk Products
Bacteria Acidoláctica
Probióticos
Genómica
Experimentación in Vitro
Productos Lácteos
Lacticaseibacillus rhamnosus - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/21132
Ver los metadatos del registro completo
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Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterizationDe Gregorio, Priscilla RominaLieske, IsadoraNied, CathySignori, GabrielaKuhn, DanielSchmitz, CarolineMartins, Vera Lúcia MilaniBustamante Filho, Ivan CunhaIrazoqui, Jose MatiasSouza, Claucia Fernanda Volken deLactic Acid BacteriaProbioticsGenomicsMilk ProductsBacteria AcidolácticaProbióticosGenómicaExperimentación in VitroProductos LácteosLacticaseibacillus rhamnosusThis study aimed to identify promising probiotic candidates among 25 autochthonous lactic acid bacteria (LAB) isolates from dairy products sourced from southeastern Brazil. In vitro assays were carried out to elucidate their phenotypic profiles, focusing on key probiotic attributes (antimicrobial, antioxidant and β-galactosidase activities, resistance to simulated gastrointestinal conditions, antibiotic susceptibility, and adhesion capacity). Principal Component Analysis was subsequently employed to rank the most promising strains. Among the isolates, four Lacticaseibacillus rhamnosus strains (6QLR5, GTM7, CLM11, and JLR3) demonstrated promising antimicrobial activity, adhesion capacity, tolerance to gastrointestinal conditions, antioxidant capacity, and β-galactosidase activity. Of these, strains 6QLR5 and GTM7 displayed the most robust phenotypic probiotic profiles and were selected for in silico genomic analysis. Functional annotation revealed genes associated with key probiotic traits, including redox systems related to antioxidant activity, adhesion-associated proteins, and mechanisms of resistance and adaptation to the harsh conditions of the intestinal tract. Genomic safety assessment, encompassing antimicrobial resistance genes, mobile genetic elements, virulence factors, and genome-stability features, indicated that strains 6QLR5 and GTM7 are safe probiotic candidates, as they harbored no genes linked to antibiotic resistance, pathogenicity, or virulence, and lacked hazardous plasmids or prophages. Overall, the characterization of autochthonous LAB strains based on integrative in vitro and in silico approaches proved effective for selecting probiotic candidates.EEA RafaelaFil: De Gregorio, Priscilla Romina. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; BrasilFil: De Gregorio, Priscilla Romina. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; BrasilFil: De Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Referencia para Lactobacilos (CERELA); ArgentinaFil: Lieske, Isadora. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; BrasilFil: Lieske, Isadora. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; BrasilFil: Nied, Cathy. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; BrasilFil: Signori, Gabriela. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; BrasilFil: Kuhn, Daniel. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; BrasilFil: Schmitz, Caroline. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; BrasilFil: Martins, Vera Lúcia Milani. Federal Institute of Education, Science and Technology of Rio Grande Do Sul; BrasilFil: Bustamante Filho, Ivan Cunha. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; BrasilFil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; ArgentinaFil: Souza, Claucia Fernanda Volken de. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; BrasilFil: Souza, Claucia Fernanda Volken de. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; BrasilIn Vitro ExperimentationElsevier2025-02-05T11:47:06Z2025-02-05T11:47:06Z2026-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21132https://www.sciencedirect.com/science/article/abs/pii/S22124292250224482212-42922212-4306https://doi.org/10.1016/j.fbio.2025.108066Food Bioscience 75 : 108066. (January 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:46:57Zoai:localhost:20.500.12123/21132instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:46:58.165INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
| dc.title.none.fl_str_mv |
Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| title |
Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| spellingShingle |
Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization De Gregorio, Priscilla Romina Lactic Acid Bacteria Probiotics Genomics Milk Products Bacteria Acidoláctica Probióticos Genómica Experimentación in Vitro Productos Lácteos Lacticaseibacillus rhamnosus |
| title_short |
Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| title_full |
Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| title_fullStr |
Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| title_full_unstemmed |
Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| title_sort |
Autochthonous Lacticaseibacillus rhamnosus strains as probiotic candidates: comprehensive in vitro and in silico characterization |
| dc.creator.none.fl_str_mv |
De Gregorio, Priscilla Romina Lieske, Isadora Nied, Cathy Signori, Gabriela Kuhn, Daniel Schmitz, Caroline Martins, Vera Lúcia Milani Bustamante Filho, Ivan Cunha Irazoqui, Jose Matias Souza, Claucia Fernanda Volken de |
| author |
De Gregorio, Priscilla Romina |
| author_facet |
De Gregorio, Priscilla Romina Lieske, Isadora Nied, Cathy Signori, Gabriela Kuhn, Daniel Schmitz, Caroline Martins, Vera Lúcia Milani Bustamante Filho, Ivan Cunha Irazoqui, Jose Matias Souza, Claucia Fernanda Volken de |
| author_role |
author |
| author2 |
Lieske, Isadora Nied, Cathy Signori, Gabriela Kuhn, Daniel Schmitz, Caroline Martins, Vera Lúcia Milani Bustamante Filho, Ivan Cunha Irazoqui, Jose Matias Souza, Claucia Fernanda Volken de |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Lactic Acid Bacteria Probiotics Genomics Milk Products Bacteria Acidoláctica Probióticos Genómica Experimentación in Vitro Productos Lácteos Lacticaseibacillus rhamnosus |
| topic |
Lactic Acid Bacteria Probiotics Genomics Milk Products Bacteria Acidoláctica Probióticos Genómica Experimentación in Vitro Productos Lácteos Lacticaseibacillus rhamnosus |
| dc.description.none.fl_txt_mv |
This study aimed to identify promising probiotic candidates among 25 autochthonous lactic acid bacteria (LAB) isolates from dairy products sourced from southeastern Brazil. In vitro assays were carried out to elucidate their phenotypic profiles, focusing on key probiotic attributes (antimicrobial, antioxidant and β-galactosidase activities, resistance to simulated gastrointestinal conditions, antibiotic susceptibility, and adhesion capacity). Principal Component Analysis was subsequently employed to rank the most promising strains. Among the isolates, four Lacticaseibacillus rhamnosus strains (6QLR5, GTM7, CLM11, and JLR3) demonstrated promising antimicrobial activity, adhesion capacity, tolerance to gastrointestinal conditions, antioxidant capacity, and β-galactosidase activity. Of these, strains 6QLR5 and GTM7 displayed the most robust phenotypic probiotic profiles and were selected for in silico genomic analysis. Functional annotation revealed genes associated with key probiotic traits, including redox systems related to antioxidant activity, adhesion-associated proteins, and mechanisms of resistance and adaptation to the harsh conditions of the intestinal tract. Genomic safety assessment, encompassing antimicrobial resistance genes, mobile genetic elements, virulence factors, and genome-stability features, indicated that strains 6QLR5 and GTM7 are safe probiotic candidates, as they harbored no genes linked to antibiotic resistance, pathogenicity, or virulence, and lacked hazardous plasmids or prophages. Overall, the characterization of autochthonous LAB strains based on integrative in vitro and in silico approaches proved effective for selecting probiotic candidates. EEA Rafaela Fil: De Gregorio, Priscilla Romina. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: De Gregorio, Priscilla Romina. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil Fil: De Gregorio, Priscilla Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Referencia para Lactobacilos (CERELA); Argentina Fil: Lieske, Isadora. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Lieske, Isadora. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil Fil: Nied, Cathy. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Signori, Gabriela. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Kuhn, Daniel. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Schmitz, Caroline. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Martins, Vera Lúcia Milani. Federal Institute of Education, Science and Technology of Rio Grande Do Sul; Brasil Fil: Bustamante Filho, Ivan Cunha. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil Fil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Irazoqui, Jose Matias. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Souza, Claucia Fernanda Volken de. University of Vale Do Taquari - Univates. Food Biotechnology Laboratory; Brasil Fil: Souza, Claucia Fernanda Volken de. University of Vale Do Taquari - Univates. Biotechnology Graduate Program; Brasil In Vitro Experimentation |
| description |
This study aimed to identify promising probiotic candidates among 25 autochthonous lactic acid bacteria (LAB) isolates from dairy products sourced from southeastern Brazil. In vitro assays were carried out to elucidate their phenotypic profiles, focusing on key probiotic attributes (antimicrobial, antioxidant and β-galactosidase activities, resistance to simulated gastrointestinal conditions, antibiotic susceptibility, and adhesion capacity). Principal Component Analysis was subsequently employed to rank the most promising strains. Among the isolates, four Lacticaseibacillus rhamnosus strains (6QLR5, GTM7, CLM11, and JLR3) demonstrated promising antimicrobial activity, adhesion capacity, tolerance to gastrointestinal conditions, antioxidant capacity, and β-galactosidase activity. Of these, strains 6QLR5 and GTM7 displayed the most robust phenotypic probiotic profiles and were selected for in silico genomic analysis. Functional annotation revealed genes associated with key probiotic traits, including redox systems related to antioxidant activity, adhesion-associated proteins, and mechanisms of resistance and adaptation to the harsh conditions of the intestinal tract. Genomic safety assessment, encompassing antimicrobial resistance genes, mobile genetic elements, virulence factors, and genome-stability features, indicated that strains 6QLR5 and GTM7 are safe probiotic candidates, as they harbored no genes linked to antibiotic resistance, pathogenicity, or virulence, and lacked hazardous plasmids or prophages. Overall, the characterization of autochthonous LAB strains based on integrative in vitro and in silico approaches proved effective for selecting probiotic candidates. |
| publishDate |
2025 |
| dc.date.none.fl_str_mv |
2025-02-05T11:47:06Z 2025-02-05T11:47:06Z 2026-01 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/20.500.12123/21132 https://www.sciencedirect.com/science/article/abs/pii/S2212429225022448 2212-4292 2212-4306 https://doi.org/10.1016/j.fbio.2025.108066 |
| url |
http://hdl.handle.net/20.500.12123/21132 https://www.sciencedirect.com/science/article/abs/pii/S2212429225022448 https://doi.org/10.1016/j.fbio.2025.108066 |
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2212-4292 2212-4306 |
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eng |
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eng |
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info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier |
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Elsevier |
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Food Bioscience 75 : 108066. (January 2026) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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