Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer

Autores
Lenoir, Marion; del Carmen, Silvina Andrea; Cortes Perez, Naima G.; Lozano Ojalvo, Daniel; Muñoz Provencio, Diego; Chain, Florian; Langella, Philippe; de Moreno, Maria Alejandra; Leblanc, Jean Guy Joseph; Bermudez Humaran, Luis G.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Chronic intestinal inflammation alters host physiology and could lead to colorectal cancer (CRC). We have previously reported beneficial effects of the probiotic strain of Lactobacillus casei BL23 in different murine models of intestinal inflammation. In addition, there is an emerging interest on the potential beneficial effects of probiotics to treat CRC. We thus explored whether L. casei BL23 displays protective effects on CRC. Methods: Mice were subcutaneously injected with 1,2-dimethylhydrazine (DMH) weekly during 10 weeks and orally administered with L. casei BL23 in the drinking water until the 10th week. Multiple plaque lesions in the large intestine were observed macroscopically and counted and intestinal tissues were also histologically analyzed. Finally, T-cell populations and cytokine production were evaluated after co-incubation of L. casei BL23 with spleen cells from non-treated mice to determine the immuno-modulatory effects of this bacterium. Results: Our results show that oral treatment with this probiotic bacterium modulates host immune responses and significantly protect mice against DMH-induced CRC. This protection may be associated with the modulation of regulatory T-cells towards a Th17-biased immune response accompanied by the expression of regulatory cytokines (IL-6, IL-17, IL-10 and TGF-β), as demonstrated in L. casei BL23-treated splenocytes, but also with the colonic expression of IL-22 observed in vivo on L. casei BL23-treated mice; suggesting the induction of a fine-tune Th17-biased response. Conclusions: Altogether our results reveal the high potential of L. casei BL23 to treat CRC and opens new frontiers for the study of immunomodulatory functions of probiotics.
Fil: Lenoir, Marion. Institut National de la Recherche Agronomique; Francia. AgroParisTech; Francia
Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Cortes Perez, Naima G.. Institut National de la Recherche Agronomique; Francia
Fil: Lozano Ojalvo, Daniel. Institut National de la Recherche Agronomique; Francia. Service de Pharmacologie et d’Immunoanalyse. CEA, IBiTecS; Francia. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Muñoz Provencio, Diego. Institut National de la Recherche Agronomique; Francia
Fil: Chain, Florian. Institut National de la Recherche Agronomique; Francia
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Bermudez Humaran, Luis G.. Institut National de la Recherche Agronomique; Francia
Materia
Colorectal Cancer
Lactic Acid Bacteria
Lactobacillus Casei Bl23
Probiotics
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/56299
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/56299
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancerLenoir, Mariondel Carmen, Silvina AndreaCortes Perez, Naima G.Lozano Ojalvo, DanielMuñoz Provencio, DiegoChain, FlorianLangella, Philippede Moreno, Maria AlejandraLeblanc, Jean Guy JosephBermudez Humaran, Luis G.Colorectal CancerLactic Acid BacteriaLactobacillus Casei Bl23Probioticshttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3Background: Chronic intestinal inflammation alters host physiology and could lead to colorectal cancer (CRC). We have previously reported beneficial effects of the probiotic strain of Lactobacillus casei BL23 in different murine models of intestinal inflammation. In addition, there is an emerging interest on the potential beneficial effects of probiotics to treat CRC. We thus explored whether L. casei BL23 displays protective effects on CRC. Methods: Mice were subcutaneously injected with 1,2-dimethylhydrazine (DMH) weekly during 10 weeks and orally administered with L. casei BL23 in the drinking water until the 10th week. Multiple plaque lesions in the large intestine were observed macroscopically and counted and intestinal tissues were also histologically analyzed. Finally, T-cell populations and cytokine production were evaluated after co-incubation of L. casei BL23 with spleen cells from non-treated mice to determine the immuno-modulatory effects of this bacterium. Results: Our results show that oral treatment with this probiotic bacterium modulates host immune responses and significantly protect mice against DMH-induced CRC. This protection may be associated with the modulation of regulatory T-cells towards a Th17-biased immune response accompanied by the expression of regulatory cytokines (IL-6, IL-17, IL-10 and TGF-β), as demonstrated in L. casei BL23-treated splenocytes, but also with the colonic expression of IL-22 observed in vivo on L. casei BL23-treated mice; suggesting the induction of a fine-tune Th17-biased response. Conclusions: Altogether our results reveal the high potential of L. casei BL23 to treat CRC and opens new frontiers for the study of immunomodulatory functions of probiotics.Fil: Lenoir, Marion. Institut National de la Recherche Agronomique; Francia. AgroParisTech; FranciaFil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Cortes Perez, Naima G.. Institut National de la Recherche Agronomique; FranciaFil: Lozano Ojalvo, Daniel. Institut National de la Recherche Agronomique; Francia. Service de Pharmacologie et d’Immunoanalyse. CEA, IBiTecS; Francia. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; EspañaFil: Muñoz Provencio, Diego. Institut National de la Recherche Agronomique; FranciaFil: Chain, Florian. Institut National de la Recherche Agronomique; FranciaFil: Langella, Philippe. Institut National de la Recherche Agronomique; FranciaFil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Bermudez Humaran, Luis G.. Institut National de la Recherche Agronomique; FranciaSpringer Tokyo2016-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/56299Lenoir, Marion; del Carmen, Silvina Andrea; Cortes Perez, Naima G.; Lozano Ojalvo, Daniel; Muñoz Provencio, Diego; et al.; Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer; Springer Tokyo; Journal Of Gastroenterology; 51; 9; 9-2016; 862-8730944-11741435-5922CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s00535-015-1158-9info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00535-015-1158-9info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:51Zoai:ri.conicet.gov.ar:11336/56299instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:51.541CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer
title Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer
spellingShingle Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer
Lenoir, Marion
Colorectal Cancer
Lactic Acid Bacteria
Lactobacillus Casei Bl23
Probiotics
title_short Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer
title_full Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer
title_fullStr Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer
title_full_unstemmed Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer
title_sort Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer
dc.creator.none.fl_str_mv Lenoir, Marion
del Carmen, Silvina Andrea
Cortes Perez, Naima G.
Lozano Ojalvo, Daniel
Muñoz Provencio, Diego
Chain, Florian
Langella, Philippe
de Moreno, Maria Alejandra
Leblanc, Jean Guy Joseph
Bermudez Humaran, Luis G.
author Lenoir, Marion
author_facet Lenoir, Marion
del Carmen, Silvina Andrea
Cortes Perez, Naima G.
Lozano Ojalvo, Daniel
Muñoz Provencio, Diego
Chain, Florian
Langella, Philippe
de Moreno, Maria Alejandra
Leblanc, Jean Guy Joseph
Bermudez Humaran, Luis G.
author_role author
author2 del Carmen, Silvina Andrea
Cortes Perez, Naima G.
Lozano Ojalvo, Daniel
Muñoz Provencio, Diego
Chain, Florian
Langella, Philippe
de Moreno, Maria Alejandra
Leblanc, Jean Guy Joseph
Bermudez Humaran, Luis G.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Colorectal Cancer
Lactic Acid Bacteria
Lactobacillus Casei Bl23
Probiotics
topic Colorectal Cancer
Lactic Acid Bacteria
Lactobacillus Casei Bl23
Probiotics
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Chronic intestinal inflammation alters host physiology and could lead to colorectal cancer (CRC). We have previously reported beneficial effects of the probiotic strain of Lactobacillus casei BL23 in different murine models of intestinal inflammation. In addition, there is an emerging interest on the potential beneficial effects of probiotics to treat CRC. We thus explored whether L. casei BL23 displays protective effects on CRC. Methods: Mice were subcutaneously injected with 1,2-dimethylhydrazine (DMH) weekly during 10 weeks and orally administered with L. casei BL23 in the drinking water until the 10th week. Multiple plaque lesions in the large intestine were observed macroscopically and counted and intestinal tissues were also histologically analyzed. Finally, T-cell populations and cytokine production were evaluated after co-incubation of L. casei BL23 with spleen cells from non-treated mice to determine the immuno-modulatory effects of this bacterium. Results: Our results show that oral treatment with this probiotic bacterium modulates host immune responses and significantly protect mice against DMH-induced CRC. This protection may be associated with the modulation of regulatory T-cells towards a Th17-biased immune response accompanied by the expression of regulatory cytokines (IL-6, IL-17, IL-10 and TGF-β), as demonstrated in L. casei BL23-treated splenocytes, but also with the colonic expression of IL-22 observed in vivo on L. casei BL23-treated mice; suggesting the induction of a fine-tune Th17-biased response. Conclusions: Altogether our results reveal the high potential of L. casei BL23 to treat CRC and opens new frontiers for the study of immunomodulatory functions of probiotics.
Fil: Lenoir, Marion. Institut National de la Recherche Agronomique; Francia. AgroParisTech; Francia
Fil: del Carmen, Silvina Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Cortes Perez, Naima G.. Institut National de la Recherche Agronomique; Francia
Fil: Lozano Ojalvo, Daniel. Institut National de la Recherche Agronomique; Francia. Service de Pharmacologie et d’Immunoanalyse. CEA, IBiTecS; Francia. Consejo Superior de Investigaciones Científicas. Instituto de Investigación en Ciencias de la Alimentación; España
Fil: Muñoz Provencio, Diego. Institut National de la Recherche Agronomique; Francia
Fil: Chain, Florian. Institut National de la Recherche Agronomique; Francia
Fil: Langella, Philippe. Institut National de la Recherche Agronomique; Francia
Fil: de Moreno, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Leblanc, Jean Guy Joseph. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Bermudez Humaran, Luis G.. Institut National de la Recherche Agronomique; Francia
description Background: Chronic intestinal inflammation alters host physiology and could lead to colorectal cancer (CRC). We have previously reported beneficial effects of the probiotic strain of Lactobacillus casei BL23 in different murine models of intestinal inflammation. In addition, there is an emerging interest on the potential beneficial effects of probiotics to treat CRC. We thus explored whether L. casei BL23 displays protective effects on CRC. Methods: Mice were subcutaneously injected with 1,2-dimethylhydrazine (DMH) weekly during 10 weeks and orally administered with L. casei BL23 in the drinking water until the 10th week. Multiple plaque lesions in the large intestine were observed macroscopically and counted and intestinal tissues were also histologically analyzed. Finally, T-cell populations and cytokine production were evaluated after co-incubation of L. casei BL23 with spleen cells from non-treated mice to determine the immuno-modulatory effects of this bacterium. Results: Our results show that oral treatment with this probiotic bacterium modulates host immune responses and significantly protect mice against DMH-induced CRC. This protection may be associated with the modulation of regulatory T-cells towards a Th17-biased immune response accompanied by the expression of regulatory cytokines (IL-6, IL-17, IL-10 and TGF-β), as demonstrated in L. casei BL23-treated splenocytes, but also with the colonic expression of IL-22 observed in vivo on L. casei BL23-treated mice; suggesting the induction of a fine-tune Th17-biased response. Conclusions: Altogether our results reveal the high potential of L. casei BL23 to treat CRC and opens new frontiers for the study of immunomodulatory functions of probiotics.
publishDate 2016
dc.date.none.fl_str_mv 2016-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/56299
Lenoir, Marion; del Carmen, Silvina Andrea; Cortes Perez, Naima G.; Lozano Ojalvo, Daniel; Muñoz Provencio, Diego; et al.; Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer; Springer Tokyo; Journal Of Gastroenterology; 51; 9; 9-2016; 862-873
0944-1174
1435-5922
CONICET Digital
CONICET
url http://hdl.handle.net/11336/56299
identifier_str_mv Lenoir, Marion; del Carmen, Silvina Andrea; Cortes Perez, Naima G.; Lozano Ojalvo, Daniel; Muñoz Provencio, Diego; et al.; Lactobacillus casei BL23 regulates Treg and Th17 T-cell populations and reduces DMH-associated colorectal cancer; Springer Tokyo; Journal Of Gastroenterology; 51; 9; 9-2016; 862-873
0944-1174
1435-5922
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s00535-015-1158-9
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs00535-015-1158-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer Tokyo
publisher.none.fl_str_mv Springer Tokyo
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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