Genetic dissection of vitamin E biosynthesis in tomato

Autores
Almeida de Souza, Juliana Beatriz; Quadrana, Leandro Daniel; Asis, Ramón; Setta, Nathalia; Godoy, Fabiana de; Bermúdez, Luisa; Otaiza, Santiago N.; Silva, Junia V. Corrêa da; Fernie, Alisdair R.; Carrari, Fernando; Rossi, Magdalena
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for α-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (α, β, γ, and δ) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical.
Instituto de Biotecnología
Fil: Almeida de Souza, Juliana Beatriz. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Setta, Nathalia. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Godoy, Fabiana de. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Bermúdez, Luisa. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Otaiza, Santiago N. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Silva, Junia V. Corrêa da. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Fernie, Alisdair R. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Rossi, Magdalena. Universidade de São Pablo. Instituto de Biociências. Departamento de Botânica; Brasil
Fuente
Journal of Experimental Botany 62 (11) : 3781–3798. (July 2011)
Materia
Tomate
Genética
Tocoferoles
Vitamina E
Loci de Rasgos Cuantitativos
Tomatoes
Genetics
Tocopherols
Vitamin E
Quantitative Trait Loci
QTL (quantitative trait loci)
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7492
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7492
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network_name_str INTA Digital (INTA)
spelling Genetic dissection of vitamin E biosynthesis in tomatoAlmeida de Souza, Juliana BeatrizQuadrana, Leandro DanielAsis, RamónSetta, NathaliaGodoy, Fabiana deBermúdez, LuisaOtaiza, Santiago N.Silva, Junia V. Corrêa daFernie, Alisdair R.Carrari, FernandoRossi, MagdalenaTomateGenéticaTocoferolesVitamina ELoci de Rasgos CuantitativosTomatoesGeneticsTocopherolsVitamin EQuantitative Trait LociQTL (quantitative trait loci)Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for α-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (α, β, γ, and δ) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical.Instituto de BiotecnologíaFil: Almeida de Souza, Juliana Beatriz. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Setta, Nathalia. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Godoy, Fabiana de. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Bermúdez, Luisa. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Otaiza, Santiago N. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; ArgentinaFil: Silva, Junia V. Corrêa da. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; BrasilFil: Fernie, Alisdair R. Max Planck Institute for Molecular Plant Physiology; AlemaniaFil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Rossi, Magdalena. Universidade de São Pablo. Instituto de Biociências. Departamento de Botânica; BrasilSociety for Experimental Biology2020-06-30T14:02:12Z2020-06-30T14:02:12Z2011-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7492https://academic.oup.com/jxb/article/62/11/3781/5018900022-09571460-2431https://doi.org/10.1093/jxb/err055Journal of Experimental Botany 62 (11) : 3781–3798. (July 2011)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:48:28Zoai:localhost:20.500.12123/7492instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:30.794INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic dissection of vitamin E biosynthesis in tomato
title Genetic dissection of vitamin E biosynthesis in tomato
spellingShingle Genetic dissection of vitamin E biosynthesis in tomato
Almeida de Souza, Juliana Beatriz
Tomate
Genética
Tocoferoles
Vitamina E
Loci de Rasgos Cuantitativos
Tomatoes
Genetics
Tocopherols
Vitamin E
Quantitative Trait Loci
QTL (quantitative trait loci)
title_short Genetic dissection of vitamin E biosynthesis in tomato
title_full Genetic dissection of vitamin E biosynthesis in tomato
title_fullStr Genetic dissection of vitamin E biosynthesis in tomato
title_full_unstemmed Genetic dissection of vitamin E biosynthesis in tomato
title_sort Genetic dissection of vitamin E biosynthesis in tomato
dc.creator.none.fl_str_mv Almeida de Souza, Juliana Beatriz
Quadrana, Leandro Daniel
Asis, Ramón
Setta, Nathalia
Godoy, Fabiana de
Bermúdez, Luisa
Otaiza, Santiago N.
Silva, Junia V. Corrêa da
Fernie, Alisdair R.
Carrari, Fernando
Rossi, Magdalena
author Almeida de Souza, Juliana Beatriz
author_facet Almeida de Souza, Juliana Beatriz
Quadrana, Leandro Daniel
Asis, Ramón
Setta, Nathalia
Godoy, Fabiana de
Bermúdez, Luisa
Otaiza, Santiago N.
Silva, Junia V. Corrêa da
Fernie, Alisdair R.
Carrari, Fernando
Rossi, Magdalena
author_role author
author2 Quadrana, Leandro Daniel
Asis, Ramón
Setta, Nathalia
Godoy, Fabiana de
Bermúdez, Luisa
Otaiza, Santiago N.
Silva, Junia V. Corrêa da
Fernie, Alisdair R.
Carrari, Fernando
Rossi, Magdalena
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tomate
Genética
Tocoferoles
Vitamina E
Loci de Rasgos Cuantitativos
Tomatoes
Genetics
Tocopherols
Vitamin E
Quantitative Trait Loci
QTL (quantitative trait loci)
topic Tomate
Genética
Tocoferoles
Vitamina E
Loci de Rasgos Cuantitativos
Tomatoes
Genetics
Tocopherols
Vitamin E
Quantitative Trait Loci
QTL (quantitative trait loci)
dc.description.none.fl_txt_mv Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for α-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (α, β, γ, and δ) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical.
Instituto de Biotecnología
Fil: Almeida de Souza, Juliana Beatriz. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Quadrana, Leandro Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asis, Ramón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Setta, Nathalia. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Godoy, Fabiana de. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Bermúdez, Luisa. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Otaiza, Santiago N. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas; Argentina
Fil: Silva, Junia V. Corrêa da. Universidade de Sao Paulo. Instituto de Biociências. Departamento de Botânica; Brasil
Fil: Fernie, Alisdair R. Max Planck Institute for Molecular Plant Physiology; Alemania
Fil: Carrari, Fernando Oscar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Rossi, Magdalena. Universidade de São Pablo. Instituto de Biociências. Departamento de Botânica; Brasil
description Vegetables are critical for human health as they are a source of multiple vitamins including vitamin E (VTE). In plants, the synthesis of VTE compounds, tocopherol and tocotrienol, derives from precursors of the shikimate and methylerythritol phosphate pathways. Quantitative trait loci (QTL) for α-tocopherol content in ripe fruit have previously been determined in an Solanum pennellii tomato introgression line population. In this work, variations of tocopherol isoforms (α, β, γ, and δ) in ripe fruits of these lines were studied. In parallel all tomato genes structurally associated with VTE biosynthesis were identified and mapped. Previously identified VTE QTL on chromosomes 6 and 9 were confirmed whilst novel ones were identified on chromosomes 7 and 8. Integrated analysis at the metabolic, genetic and genomic levels allowed us to propose 16 candidate loci putatively affecting tocopherol content in tomato. A comparative analysis revealed polymorphisms at nucleotide and amino acid levels between Solanum lycopersicum and S. pennellii candidate alleles. Moreover, evolutionary analyses showed the presence of codons evolving under both neutral and positive selection, which may explain the phenotypic differences between species. These data represent an important step in understanding the genetic determinants of VTE natural variation in tomato fruit and as such in the ability to improve the content of this important nutriceutical.
publishDate 2011
dc.date.none.fl_str_mv 2011-07
2020-06-30T14:02:12Z
2020-06-30T14:02:12Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7492
https://academic.oup.com/jxb/article/62/11/3781/501890
0022-0957
1460-2431
https://doi.org/10.1093/jxb/err055
url http://hdl.handle.net/20.500.12123/7492
https://academic.oup.com/jxb/article/62/11/3781/501890
https://doi.org/10.1093/jxb/err055
identifier_str_mv 0022-0957
1460-2431
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Society for Experimental Biology
publisher.none.fl_str_mv Society for Experimental Biology
dc.source.none.fl_str_mv Journal of Experimental Botany 62 (11) : 3781–3798. (July 2011)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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