Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds

Autores
Nunes Nesi, Adriano; Alseekh, Saleh; Oliveira Silva, Franklin Magnum de; Omranian, Nooshin; Lichtenstein, Gabriel; Mirnezhad, Mohammad; Romero González, Roman R.; Sabio Y Garcia, Julia Veronica; Conte, Mariana; Leiss, Kirsten A.; Klinkhamer, Peter G. L.; Nikoloski, Zoran; Carrari, Fernando; Fernie, Alisdair R.
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective: This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods: The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results: Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions: Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.
Instituto de Biotecnología
Fil: Nunes Nesi, Adriano. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; Brasil. Max-Planck-Institute of Molecular Plant Physiology; Alemania
Fil: Alseekh, Saleh. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; Bulgaria
Fil: Oliveira Silva, Franklin Magnum de. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; Brasil
Fil: Omranian, Nooshin. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; Bulgaria
Fil: Lichtenstein, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Mirnezhad, Mohammad. Leiden University. Plant Ecology, Institute of Biology; Holanda
Fil: Romero González, Roman R. Leiden University. Plant Ecology, Institute of Biology; Holanda
Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Conte, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Leiss, Kirsten A. Leiden University. Plant Ecology, Institute of Biology; Holanda. Wageningen University & Research. Business Unit Horticulture; Holanda
Fil: Klinkhamer, Peter G. L. Leiden University. Plant Ecology, Institute of Biology; Holanda
Fil: Nikoloski, Zoran. Max-Planck-Institute of Molecular Plant Physiology; Alemania. University of Potsdam. Institute of Biochemistry and Biology. Bioinformatics Group; Alemania
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Fernie, Alisdair R. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; Bulgaria
Fuente
Metabolomics 15 : 46. (April 2019)
Materia
Tomate
Solanum Lycopersicum
Metabolitos
Frutas
Semillas
Tomatoes
Quantitative Trait Loci
Loci de Rasgos Cuantitativos
Metabolites
Fruit
Seed
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4655
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4655
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seedsNunes Nesi, AdrianoAlseekh, SalehOliveira Silva, Franklin Magnum deOmranian, NooshinLichtenstein, GabrielMirnezhad, MohammadRomero González, Roman R.Sabio Y Garcia, Julia VeronicaConte, MarianaLeiss, Kirsten A.Klinkhamer, Peter G. L.Nikoloski, ZoranCarrari, FernandoFernie, Alisdair R.TomateSolanum LycopersicumMetabolitosFrutasSemillasTomatoesQuantitative Trait LociLoci de Rasgos CuantitativosMetabolitesFruitSeedIntroduction: To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective: This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods: The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results: Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions: Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.Instituto de BiotecnologíaFil: Nunes Nesi, Adriano. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; Brasil. Max-Planck-Institute of Molecular Plant Physiology; AlemaniaFil: Alseekh, Saleh. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; BulgariaFil: Oliveira Silva, Franklin Magnum de. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; BrasilFil: Omranian, Nooshin. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; BulgariaFil: Lichtenstein, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Mirnezhad, Mohammad. Leiden University. Plant Ecology, Institute of Biology; HolandaFil: Romero González, Roman R. Leiden University. Plant Ecology, Institute of Biology; HolandaFil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Conte, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Leiss, Kirsten A. Leiden University. Plant Ecology, Institute of Biology; Holanda. Wageningen University & Research. Business Unit Horticulture; HolandaFil: Klinkhamer, Peter G. L. Leiden University. Plant Ecology, Institute of Biology; HolandaFil: Nikoloski, Zoran. Max-Planck-Institute of Molecular Plant Physiology; Alemania. University of Potsdam. Institute of Biochemistry and Biology. Bioinformatics Group; AlemaniaFil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Fernie, Alisdair R. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; BulgariaSpringer2019-03-19T14:16:37Z2019-03-19T14:16:37Z2019-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/46551573-38821573-3890 (Online)https://doi.org/10.1007/s11306-019-1503-8Metabolomics 15 : 46. (April 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:36Zoai:localhost:20.500.12123/4655instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:36.655INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
spellingShingle Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
Nunes Nesi, Adriano
Tomate
Solanum Lycopersicum
Metabolitos
Frutas
Semillas
Tomatoes
Quantitative Trait Loci
Loci de Rasgos Cuantitativos
Metabolites
Fruit
Seed
title_short Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_full Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_fullStr Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_full_unstemmed Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
title_sort Identification and characterization of metabolite quantitative trait loci in tomato leaves and comparison with those reported for fruits and seeds
dc.creator.none.fl_str_mv Nunes Nesi, Adriano
Alseekh, Saleh
Oliveira Silva, Franklin Magnum de
Omranian, Nooshin
Lichtenstein, Gabriel
Mirnezhad, Mohammad
Romero González, Roman R.
Sabio Y Garcia, Julia Veronica
Conte, Mariana
Leiss, Kirsten A.
Klinkhamer, Peter G. L.
Nikoloski, Zoran
Carrari, Fernando
Fernie, Alisdair R.
author Nunes Nesi, Adriano
author_facet Nunes Nesi, Adriano
Alseekh, Saleh
Oliveira Silva, Franklin Magnum de
Omranian, Nooshin
Lichtenstein, Gabriel
Mirnezhad, Mohammad
Romero González, Roman R.
Sabio Y Garcia, Julia Veronica
Conte, Mariana
Leiss, Kirsten A.
Klinkhamer, Peter G. L.
Nikoloski, Zoran
Carrari, Fernando
Fernie, Alisdair R.
author_role author
author2 Alseekh, Saleh
Oliveira Silva, Franklin Magnum de
Omranian, Nooshin
Lichtenstein, Gabriel
Mirnezhad, Mohammad
Romero González, Roman R.
Sabio Y Garcia, Julia Veronica
Conte, Mariana
Leiss, Kirsten A.
Klinkhamer, Peter G. L.
Nikoloski, Zoran
Carrari, Fernando
Fernie, Alisdair R.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tomate
Solanum Lycopersicum
Metabolitos
Frutas
Semillas
Tomatoes
Quantitative Trait Loci
Loci de Rasgos Cuantitativos
Metabolites
Fruit
Seed
topic Tomate
Solanum Lycopersicum
Metabolitos
Frutas
Semillas
Tomatoes
Quantitative Trait Loci
Loci de Rasgos Cuantitativos
Metabolites
Fruit
Seed
dc.description.none.fl_txt_mv Introduction: To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective: This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods: The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results: Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions: Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.
Instituto de Biotecnología
Fil: Nunes Nesi, Adriano. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; Brasil. Max-Planck-Institute of Molecular Plant Physiology; Alemania
Fil: Alseekh, Saleh. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; Bulgaria
Fil: Oliveira Silva, Franklin Magnum de. Universidade Federal de Viçosa. Departamento de Biologia Vegetal; Brasil
Fil: Omranian, Nooshin. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; Bulgaria
Fil: Lichtenstein, Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Mirnezhad, Mohammad. Leiden University. Plant Ecology, Institute of Biology; Holanda
Fil: Romero González, Roman R. Leiden University. Plant Ecology, Institute of Biology; Holanda
Fil: Sabio Y Garcia, Julia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Conte, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Leiss, Kirsten A. Leiden University. Plant Ecology, Institute of Biology; Holanda. Wageningen University & Research. Business Unit Horticulture; Holanda
Fil: Klinkhamer, Peter G. L. Leiden University. Plant Ecology, Institute of Biology; Holanda
Fil: Nikoloski, Zoran. Max-Planck-Institute of Molecular Plant Physiology; Alemania. University of Potsdam. Institute of Biochemistry and Biology. Bioinformatics Group; Alemania
Fil: Carrari, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Fernie, Alisdair R. Max-Planck-Institute of Molecular Plant Physiology; Alemania. Center of Plant System Biology and Biotechnology; Bulgaria
description Introduction: To date, most studies of natural variation and metabolite quantitative trait loci (mQTL) in tomato have focused on fruit metabolism, leaving aside the identification of genomic regions involved in the regulation of leaf metabolism. Objective: This study was conducted to identify leaf mQTL in tomato and to assess the association of leaf metabolites and physiological traits with the metabolite levels from other tissues. Methods: The analysis of components of leaf metabolism was performed by phenotypying 76 tomato ILs with chromosome segments of the wild species Solanum pennellii in the genetic background of a cultivated tomato (S. lycopersicum) variety M82. The plants were cultivated in two different environments in independent years and samples were harvested from mature leaves of non-flowering plants at the middle of the light period. The non-targeted metabolite profiling was obtained by gas chromatography time-of-flight mass spectrometry (GC-TOF-MS). With the data set obtained in this study and already published metabolomics data from seed and fruit, we performed QTL mapping, heritability and correlation analyses. Results: Changes in metabolite contents were evident in the ILs that are potentially important with respect to stress responses and plant physiology. By analyzing the obtained data, we identified 42 positive and 76 negative mQTL involved in carbon and nitrogen metabolism. Conclusions: Overall, these findings allowed the identification of S. lycopersicum genome regions involved in the regulation of leaf primary carbon and nitrogen metabolism, as well as the association of leaf metabolites with metabolites from seeds and fruits.
publishDate 2019
dc.date.none.fl_str_mv 2019-03-19T14:16:37Z
2019-03-19T14:16:37Z
2019-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4655
1573-3882
1573-3890 (Online)
https://doi.org/10.1007/s11306-019-1503-8
url http://hdl.handle.net/20.500.12123/4655
https://doi.org/10.1007/s11306-019-1503-8
identifier_str_mv 1573-3882
1573-3890 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Metabolomics 15 : 46. (April 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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