Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage

Autores
Ruggieri, Germán; Triassi, Agustina; Alvarez, Clarisa E.; Gola, Aldana; Wiggenhauser, Julieta; Budde, Claudio Olaf; Lara, María Valeria; Müller, Gabriela Leticia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Glycine-rich RNA-binding proteins (GR-RBPs) are involved in RNA processing and also some of them are output signals of the circadian clock. In tomato, one GR-RBP gene family (LeGRP1) is composed by three highly homologous genes (LeGRP1a-c); each one rendering three transcriptional products: the un-spliced pre-RNA (preLegrp1a-c), the mature mRNA (mLegrp1a-c) and the alternatively spliced mRNA (asLegrp1a-c). To get insight into their regulation and impact on RNA metabolism in fruits, Solanum lycopersicum cv. Micro-Tom was transformed with preLeGRP1a fused to the polygalacturonase promoter, which drives expression to fruits from the mature green stage. Our results demonstrated a complex positive regulation of LeGRPs, in which LeGRP1a overexpression led to the induction of the others LeGRP1 members. Even though the LeGRP1 transcription and the content of three LeGRPs proteins were affected, the overall LeGRP protein circadian rhythm profile was similar in transgenic and WT fruits. However, when the fruits are kept at chilling temperature after harvest, total protein content was significantly higher in transgenic than in WT fruits, and the content of some free amino acids was modified. The results obtained suggest a probable role of LeGRP1s: structural rearrangements and/or stabilization of mRNA to allow efficient processing of fruits under cold conditions.
EEA San Pedro
Fil: Ruggieri, German. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Triassi, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Álvarez, Clarisa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Gola, Aldana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Wiggenhauser, Julieta. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Lara, Maria Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Drincovich, Maria F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Müller, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fuente
Biologia Plantarum 62 (3) : 501-510 (2018)
Materia
Tomate
Solanum lycopersicum
Aminoácidos
Ritmo Circadiano
Poligalacturonasa
ARN
Almacenamiento en frío
Proteínas
Tomatoes
Amino Acids
Circadian Rhythm
Polygalacturonase
RNA
Cold Storage
Proteins
Lycopersicon esculentum
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2422
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2422
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storageRuggieri, GermánTriassi, AgustinaAlvarez, Clarisa E.Gola, AldanaWiggenhauser, JulietaBudde, Claudio OlafLara, María ValeriaMüller, Gabriela LeticiaTomateSolanum lycopersicumAminoácidosRitmo CircadianoPoligalacturonasaARNAlmacenamiento en fríoProteínasTomatoesAmino AcidsCircadian RhythmPolygalacturonaseRNACold StorageProteinsLycopersicon esculentumGlycine-rich RNA-binding proteins (GR-RBPs) are involved in RNA processing and also some of them are output signals of the circadian clock. In tomato, one GR-RBP gene family (LeGRP1) is composed by three highly homologous genes (LeGRP1a-c); each one rendering three transcriptional products: the un-spliced pre-RNA (preLegrp1a-c), the mature mRNA (mLegrp1a-c) and the alternatively spliced mRNA (asLegrp1a-c). To get insight into their regulation and impact on RNA metabolism in fruits, Solanum lycopersicum cv. Micro-Tom was transformed with preLeGRP1a fused to the polygalacturonase promoter, which drives expression to fruits from the mature green stage. Our results demonstrated a complex positive regulation of LeGRPs, in which LeGRP1a overexpression led to the induction of the others LeGRP1 members. Even though the LeGRP1 transcription and the content of three LeGRPs proteins were affected, the overall LeGRP protein circadian rhythm profile was similar in transgenic and WT fruits. However, when the fruits are kept at chilling temperature after harvest, total protein content was significantly higher in transgenic than in WT fruits, and the content of some free amino acids was modified. The results obtained suggest a probable role of LeGRP1s: structural rearrangements and/or stabilization of mRNA to allow efficient processing of fruits under cold conditions.EEA San PedroFil: Ruggieri, German. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Triassi, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Álvarez, Clarisa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Gola, Aldana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Wiggenhauser, Julieta. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; ArgentinaFil: Lara, Maria Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Drincovich, Maria F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Müller, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina2018-05-17T17:34:15Z2018-05-17T17:34:15Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007%2Fs10535-018-0794-3http://hdl.handle.net/20.500.12123/24221573-82640006-3134https://doi.org/10.1007/s10535-018-0794-3Biologia Plantarum 62 (3) : 501-510 (2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:18Zoai:localhost:20.500.12123/2422instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:19.118INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage
title Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage
spellingShingle Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage
Ruggieri, Germán
Tomate
Solanum lycopersicum
Aminoácidos
Ritmo Circadiano
Poligalacturonasa
ARN
Almacenamiento en frío
Proteínas
Tomatoes
Amino Acids
Circadian Rhythm
Polygalacturonase
RNA
Cold Storage
Proteins
Lycopersicon esculentum
title_short Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage
title_full Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage
title_fullStr Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage
title_full_unstemmed Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage
title_sort Overexpression of glycine-rich RNA-binding protein in tomato renders fruits with higher protein content after cold storage
dc.creator.none.fl_str_mv Ruggieri, Germán
Triassi, Agustina
Alvarez, Clarisa E.
Gola, Aldana
Wiggenhauser, Julieta
Budde, Claudio Olaf
Lara, María Valeria
Müller, Gabriela Leticia
author Ruggieri, Germán
author_facet Ruggieri, Germán
Triassi, Agustina
Alvarez, Clarisa E.
Gola, Aldana
Wiggenhauser, Julieta
Budde, Claudio Olaf
Lara, María Valeria
Müller, Gabriela Leticia
author_role author
author2 Triassi, Agustina
Alvarez, Clarisa E.
Gola, Aldana
Wiggenhauser, Julieta
Budde, Claudio Olaf
Lara, María Valeria
Müller, Gabriela Leticia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tomate
Solanum lycopersicum
Aminoácidos
Ritmo Circadiano
Poligalacturonasa
ARN
Almacenamiento en frío
Proteínas
Tomatoes
Amino Acids
Circadian Rhythm
Polygalacturonase
RNA
Cold Storage
Proteins
Lycopersicon esculentum
topic Tomate
Solanum lycopersicum
Aminoácidos
Ritmo Circadiano
Poligalacturonasa
ARN
Almacenamiento en frío
Proteínas
Tomatoes
Amino Acids
Circadian Rhythm
Polygalacturonase
RNA
Cold Storage
Proteins
Lycopersicon esculentum
dc.description.none.fl_txt_mv Glycine-rich RNA-binding proteins (GR-RBPs) are involved in RNA processing and also some of them are output signals of the circadian clock. In tomato, one GR-RBP gene family (LeGRP1) is composed by three highly homologous genes (LeGRP1a-c); each one rendering three transcriptional products: the un-spliced pre-RNA (preLegrp1a-c), the mature mRNA (mLegrp1a-c) and the alternatively spliced mRNA (asLegrp1a-c). To get insight into their regulation and impact on RNA metabolism in fruits, Solanum lycopersicum cv. Micro-Tom was transformed with preLeGRP1a fused to the polygalacturonase promoter, which drives expression to fruits from the mature green stage. Our results demonstrated a complex positive regulation of LeGRPs, in which LeGRP1a overexpression led to the induction of the others LeGRP1 members. Even though the LeGRP1 transcription and the content of three LeGRPs proteins were affected, the overall LeGRP protein circadian rhythm profile was similar in transgenic and WT fruits. However, when the fruits are kept at chilling temperature after harvest, total protein content was significantly higher in transgenic than in WT fruits, and the content of some free amino acids was modified. The results obtained suggest a probable role of LeGRP1s: structural rearrangements and/or stabilization of mRNA to allow efficient processing of fruits under cold conditions.
EEA San Pedro
Fil: Ruggieri, German. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Triassi, Agustina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Álvarez, Clarisa. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Gola, Aldana. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Wiggenhauser, Julieta. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Budde, Claudio Olaf. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina
Fil: Lara, Maria Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Drincovich, Maria F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Müller, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
description Glycine-rich RNA-binding proteins (GR-RBPs) are involved in RNA processing and also some of them are output signals of the circadian clock. In tomato, one GR-RBP gene family (LeGRP1) is composed by three highly homologous genes (LeGRP1a-c); each one rendering three transcriptional products: the un-spliced pre-RNA (preLegrp1a-c), the mature mRNA (mLegrp1a-c) and the alternatively spliced mRNA (asLegrp1a-c). To get insight into their regulation and impact on RNA metabolism in fruits, Solanum lycopersicum cv. Micro-Tom was transformed with preLeGRP1a fused to the polygalacturonase promoter, which drives expression to fruits from the mature green stage. Our results demonstrated a complex positive regulation of LeGRPs, in which LeGRP1a overexpression led to the induction of the others LeGRP1 members. Even though the LeGRP1 transcription and the content of three LeGRPs proteins were affected, the overall LeGRP protein circadian rhythm profile was similar in transgenic and WT fruits. However, when the fruits are kept at chilling temperature after harvest, total protein content was significantly higher in transgenic than in WT fruits, and the content of some free amino acids was modified. The results obtained suggest a probable role of LeGRP1s: structural rearrangements and/or stabilization of mRNA to allow efficient processing of fruits under cold conditions.
publishDate 2018
dc.date.none.fl_str_mv 2018-05-17T17:34:15Z
2018-05-17T17:34:15Z
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007%2Fs10535-018-0794-3
http://hdl.handle.net/20.500.12123/2422
1573-8264
0006-3134
https://doi.org/10.1007/s10535-018-0794-3
url https://link.springer.com/article/10.1007%2Fs10535-018-0794-3
http://hdl.handle.net/20.500.12123/2422
https://doi.org/10.1007/s10535-018-0794-3
identifier_str_mv 1573-8264
0006-3134
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Biologia Plantarum 62 (3) : 501-510 (2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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