Antifungal susceptibility profile and molecular identification of Cyp51C mutations in clinical and environmental isolates of Aspergillus flavus from Argentina

Autores
Hermida‐Alava, Katherine; Brito Devoto, Tomás; Sautua, Francisco José; Gordó, Manuela; Scandiani, María Mercedes; Formento, Angela Norma; Luque, Alicia; Carmona, Marcelo Anibal; Cuestas, María L.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Background: The emergence of azole resistance in non‐fumigatus Aspergillus strains is on the raise. Objectives: To study the susceptibility profiles and the molecular mechanisms of azole resistance of environmental and clinical strains of Aspergillus flavus from Argentina. Methods: Thirty‐five A flavus isolates (18 from soybean seeds and chickpea seeds and 17 from the clinic) were analysed for amphotericin B and azole resistance using the standard microbroth dilution method according to CLSI M38‐A2 guidelines. Sequencing analysis of the cyp51 genes was conducted in those isolates displaying high MICs values to itraconazole, voriconazole and/or posaconazole. Results: Among the environmental isolates, 33.3% of them showed high MIC values for at least one triazole whereas 23.5% of the clinical isolates displayed high MIC values for amphotericin B. Point mutations in the Cyp51C gene were recorded in most environmental isolates with non‐wild‐type MIC values. Conclusions: Susceptibility differences among environmental A flavus isolates might suggest the possibility of native resistance to certain triazole antifungals used in the clinic. To the best of our knowledge, this is the first report of antifungal screening of environmental strains of A flavus in soybean seeds and chickpea seeds from Argentina that showed increased resistance to voriconazole and itraconazole in comparison to clinical strains.
EEA Paraná
Fil: Hermida‐Alava, Katherine. Universidad de Buenos Aires. Instituto de Investigaciones en Microbiología y Parasitología Médica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Brito Devoto, Tomás. Universidad de Buenos Aires. Instituto de Investigaciones en Microbiología y Parasitología Médica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Sautua, Francisco José. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina
Fil: Gordó, Manuela. Laboratorio Agrícola Río Parana; Argentina
Fil: Scandiani, Mercedes M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Referencia de Micología (CEREMIC); Argentina
Fil: Formento, Angela Norma. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentina
Fil: Luque, Alicia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Referencia de Micología (CEREMIC); Argentina
Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina
Fil: Cuestas, María L. Universidad de Buenos Aires. Instituto de Investigaciones en Microbiología y Parasitología Médica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fuente
Mycoses (First published: 01 October 2020)
Materia
Aspergillus flavus
Marcadores Genéticos
Identificación
Azoles
Argentina
Genetic Markers
Identification
Marcadores Moleculares
Triazoles
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/8069
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network_name_str INTA Digital (INTA)
spelling Antifungal susceptibility profile and molecular identification of Cyp51C mutations in clinical and environmental isolates of Aspergillus flavus from ArgentinaHermida‐Alava, KatherineBrito Devoto, TomásSautua, Francisco JoséGordó, ManuelaScandiani, María MercedesFormento, Angela NormaLuque, AliciaCarmona, Marcelo AnibalCuestas, María L.Aspergillus flavusMarcadores GenéticosIdentificaciónAzolesArgentinaGenetic MarkersIdentificationMarcadores MolecularesTriazolesBackground: The emergence of azole resistance in non‐fumigatus Aspergillus strains is on the raise. Objectives: To study the susceptibility profiles and the molecular mechanisms of azole resistance of environmental and clinical strains of Aspergillus flavus from Argentina. Methods: Thirty‐five A flavus isolates (18 from soybean seeds and chickpea seeds and 17 from the clinic) were analysed for amphotericin B and azole resistance using the standard microbroth dilution method according to CLSI M38‐A2 guidelines. Sequencing analysis of the cyp51 genes was conducted in those isolates displaying high MICs values to itraconazole, voriconazole and/or posaconazole. Results: Among the environmental isolates, 33.3% of them showed high MIC values for at least one triazole whereas 23.5% of the clinical isolates displayed high MIC values for amphotericin B. Point mutations in the Cyp51C gene were recorded in most environmental isolates with non‐wild‐type MIC values. Conclusions: Susceptibility differences among environmental A flavus isolates might suggest the possibility of native resistance to certain triazole antifungals used in the clinic. To the best of our knowledge, this is the first report of antifungal screening of environmental strains of A flavus in soybean seeds and chickpea seeds from Argentina that showed increased resistance to voriconazole and itraconazole in comparison to clinical strains.EEA ParanáFil: Hermida‐Alava, Katherine. Universidad de Buenos Aires. Instituto de Investigaciones en Microbiología y Parasitología Médica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Brito Devoto, Tomás. Universidad de Buenos Aires. Instituto de Investigaciones en Microbiología y Parasitología Médica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaFil: Sautua, Francisco José. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; ArgentinaFil: Gordó, Manuela. Laboratorio Agrícola Río Parana; ArgentinaFil: Scandiani, Mercedes M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Referencia de Micología (CEREMIC); ArgentinaFil: Formento, Angela Norma. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; ArgentinaFil: Luque, Alicia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Referencia de Micología (CEREMIC); ArgentinaFil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; ArgentinaFil: Cuestas, María L. Universidad de Buenos Aires. Instituto de Investigaciones en Microbiología y Parasitología Médica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; ArgentinaWileyinfo:eu-repo/date/embargoEnd/2021-10-182020-10-16T16:14:27Z2020-10-16T16:14:27Z2020-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8069https://onlinelibrary.wiley.com/doi/abs/10.1111/myc.131930933-74071439-0507https://doi.org/10.1111/myc.13193Mycoses (First published: 01 October 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:02Zoai:localhost:20.500.12123/8069instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:03.284INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Antifungal susceptibility profile and molecular identification of Cyp51C mutations in clinical and environmental isolates of Aspergillus flavus from Argentina
title Antifungal susceptibility profile and molecular identification of Cyp51C mutations in clinical and environmental isolates of Aspergillus flavus from Argentina
spellingShingle Antifungal susceptibility profile and molecular identification of Cyp51C mutations in clinical and environmental isolates of Aspergillus flavus from Argentina
Hermida‐Alava, Katherine
Aspergillus flavus
Marcadores Genéticos
Identificación
Azoles
Argentina
Genetic Markers
Identification
Marcadores Moleculares
Triazoles
title_short Antifungal susceptibility profile and molecular identification of Cyp51C mutations in clinical and environmental isolates of Aspergillus flavus from Argentina
title_full Antifungal susceptibility profile and molecular identification of Cyp51C mutations in clinical and environmental isolates of Aspergillus flavus from Argentina
title_fullStr Antifungal susceptibility profile and molecular identification of Cyp51C mutations in clinical and environmental isolates of Aspergillus flavus from Argentina
title_full_unstemmed Antifungal susceptibility profile and molecular identification of Cyp51C mutations in clinical and environmental isolates of Aspergillus flavus from Argentina
title_sort Antifungal susceptibility profile and molecular identification of Cyp51C mutations in clinical and environmental isolates of Aspergillus flavus from Argentina
dc.creator.none.fl_str_mv Hermida‐Alava, Katherine
Brito Devoto, Tomás
Sautua, Francisco José
Gordó, Manuela
Scandiani, María Mercedes
Formento, Angela Norma
Luque, Alicia
Carmona, Marcelo Anibal
Cuestas, María L.
author Hermida‐Alava, Katherine
author_facet Hermida‐Alava, Katherine
Brito Devoto, Tomás
Sautua, Francisco José
Gordó, Manuela
Scandiani, María Mercedes
Formento, Angela Norma
Luque, Alicia
Carmona, Marcelo Anibal
Cuestas, María L.
author_role author
author2 Brito Devoto, Tomás
Sautua, Francisco José
Gordó, Manuela
Scandiani, María Mercedes
Formento, Angela Norma
Luque, Alicia
Carmona, Marcelo Anibal
Cuestas, María L.
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aspergillus flavus
Marcadores Genéticos
Identificación
Azoles
Argentina
Genetic Markers
Identification
Marcadores Moleculares
Triazoles
topic Aspergillus flavus
Marcadores Genéticos
Identificación
Azoles
Argentina
Genetic Markers
Identification
Marcadores Moleculares
Triazoles
dc.description.none.fl_txt_mv Background: The emergence of azole resistance in non‐fumigatus Aspergillus strains is on the raise. Objectives: To study the susceptibility profiles and the molecular mechanisms of azole resistance of environmental and clinical strains of Aspergillus flavus from Argentina. Methods: Thirty‐five A flavus isolates (18 from soybean seeds and chickpea seeds and 17 from the clinic) were analysed for amphotericin B and azole resistance using the standard microbroth dilution method according to CLSI M38‐A2 guidelines. Sequencing analysis of the cyp51 genes was conducted in those isolates displaying high MICs values to itraconazole, voriconazole and/or posaconazole. Results: Among the environmental isolates, 33.3% of them showed high MIC values for at least one triazole whereas 23.5% of the clinical isolates displayed high MIC values for amphotericin B. Point mutations in the Cyp51C gene were recorded in most environmental isolates with non‐wild‐type MIC values. Conclusions: Susceptibility differences among environmental A flavus isolates might suggest the possibility of native resistance to certain triazole antifungals used in the clinic. To the best of our knowledge, this is the first report of antifungal screening of environmental strains of A flavus in soybean seeds and chickpea seeds from Argentina that showed increased resistance to voriconazole and itraconazole in comparison to clinical strains.
EEA Paraná
Fil: Hermida‐Alava, Katherine. Universidad de Buenos Aires. Instituto de Investigaciones en Microbiología y Parasitología Médica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Brito Devoto, Tomás. Universidad de Buenos Aires. Instituto de Investigaciones en Microbiología y Parasitología Médica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
Fil: Sautua, Francisco José. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina
Fil: Gordó, Manuela. Laboratorio Agrícola Río Parana; Argentina
Fil: Scandiani, Mercedes M. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Referencia de Micología (CEREMIC); Argentina
Fil: Formento, Angela Norma. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Paraná; Argentina
Fil: Luque, Alicia. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Referencia de Micología (CEREMIC); Argentina
Fil: Carmona, Marcelo Anibal. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Fitopatología; Argentina
Fil: Cuestas, María L. Universidad de Buenos Aires. Instituto de Investigaciones en Microbiología y Parasitología Médica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Microbiología y Parasitología Médica; Argentina
description Background: The emergence of azole resistance in non‐fumigatus Aspergillus strains is on the raise. Objectives: To study the susceptibility profiles and the molecular mechanisms of azole resistance of environmental and clinical strains of Aspergillus flavus from Argentina. Methods: Thirty‐five A flavus isolates (18 from soybean seeds and chickpea seeds and 17 from the clinic) were analysed for amphotericin B and azole resistance using the standard microbroth dilution method according to CLSI M38‐A2 guidelines. Sequencing analysis of the cyp51 genes was conducted in those isolates displaying high MICs values to itraconazole, voriconazole and/or posaconazole. Results: Among the environmental isolates, 33.3% of them showed high MIC values for at least one triazole whereas 23.5% of the clinical isolates displayed high MIC values for amphotericin B. Point mutations in the Cyp51C gene were recorded in most environmental isolates with non‐wild‐type MIC values. Conclusions: Susceptibility differences among environmental A flavus isolates might suggest the possibility of native resistance to certain triazole antifungals used in the clinic. To the best of our knowledge, this is the first report of antifungal screening of environmental strains of A flavus in soybean seeds and chickpea seeds from Argentina that showed increased resistance to voriconazole and itraconazole in comparison to clinical strains.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-16T16:14:27Z
2020-10-16T16:14:27Z
2020-10
info:eu-repo/date/embargoEnd/2021-10-18
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8069
https://onlinelibrary.wiley.com/doi/abs/10.1111/myc.13193
0933-7407
1439-0507
https://doi.org/10.1111/myc.13193
url http://hdl.handle.net/20.500.12123/8069
https://onlinelibrary.wiley.com/doi/abs/10.1111/myc.13193
https://doi.org/10.1111/myc.13193
identifier_str_mv 0933-7407
1439-0507
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Mycoses (First published: 01 October 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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