Unlocking hidden sources of resistance to ascochyta blight in moderately resistant chickpea genotypes

Autores
Crociara, Clara Sonia; Valetti, Lucio; Castro, Patricia; Millán, Teresa; Iglesias, Juliana; Pastor, Silvina Estela
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ascochyta blight, caused by Ascochyta rabiei, is a major threat to global chickpea (Cicer arietinum L.) production, significantly reducing yield under favorable conditions. This study aimed to characterize the resistance responses of nine chickpea genotypes, previously classified as moderately resistant, by subjecting them to enhanced disease pressure. Phenotypic evaluation, including the area under the disease progress curve (AUDPC) and severity scoring was carried out. To investigate the potential genetic basis of the observed resistance response, we analyzed molecular markers previously reported as linked to quantitative trait loci (QTLs) for A. rabiei resistance. The approach focused on testing whether these established markers could distinguish genotypes following the same patterns observed in their phenotypic responses under increased pathogen pressure. The results revealed significant variability among the MR genotypes, with three genotypes FLIP06-86C, FLIP07-35C, and FLIP03-100C outperforming the resistant control. The results from hierarchical clustering (UPGMA), principal component analysis (PCA), and principal coordinate analysis (PCoA) highlighted genetic substructures consistent with observed phenotypic behaviors. However, unexpected marker-phenotype associations were detected, questioning the utility of specific markers such as SCY17 and CaETR4 in marker-assisted selection. These findings underline the complexity of polygenic resistance to A. rabiei and emphasize the importance of integrating phenotypic screening with genetic analyses to improve the reliability of chickpea breeding programs. This work also contributes to identifying superior MR genotypes, providing valuable resources for the development of resistant cultivars.
Instituto de Patología Vegetal
Fil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Castro, Patricia. Universidad de Córdoba (UCO); España
Fil: Millán, Teresa. Universidad de Córdoba (UCO); España
Fil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Pergamino; Argentina
Fil: Iglesias, Juliana.Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA), Pergamino; Argentina
Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fuente
Euphytica 221 : 108 (June 2025)
Materia
Ascochyta rabiei
Garbanzo
Tizón del garbanzo
Chickpea blight
Mejoramiento Genético
Chickpeas
Genetic Improvement
Molecular Markers
Pulse Breeding
Rabia del Garbanzo
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23020
Acceder
id INTADig_8f63dd7322f4deb72459c738c9824e7d
oai_identifier_str oai:localhost:20.500.12123/23020
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Unlocking hidden sources of resistance to ascochyta blight in moderately resistant chickpea genotypesCrociara, Clara SoniaValetti, LucioCastro, PatriciaMillán, TeresaIglesias, JulianaPastor, Silvina EstelaAscochyta rabieiGarbanzoTizón del garbanzoChickpea blightMejoramiento GenéticoChickpeasGenetic ImprovementMolecular MarkersPulse BreedingRabia del GarbanzoAscochyta blight, caused by Ascochyta rabiei, is a major threat to global chickpea (Cicer arietinum L.) production, significantly reducing yield under favorable conditions. This study aimed to characterize the resistance responses of nine chickpea genotypes, previously classified as moderately resistant, by subjecting them to enhanced disease pressure. Phenotypic evaluation, including the area under the disease progress curve (AUDPC) and severity scoring was carried out. To investigate the potential genetic basis of the observed resistance response, we analyzed molecular markers previously reported as linked to quantitative trait loci (QTLs) for A. rabiei resistance. The approach focused on testing whether these established markers could distinguish genotypes following the same patterns observed in their phenotypic responses under increased pathogen pressure. The results revealed significant variability among the MR genotypes, with three genotypes FLIP06-86C, FLIP07-35C, and FLIP03-100C outperforming the resistant control. The results from hierarchical clustering (UPGMA), principal component analysis (PCA), and principal coordinate analysis (PCoA) highlighted genetic substructures consistent with observed phenotypic behaviors. However, unexpected marker-phenotype associations were detected, questioning the utility of specific markers such as SCY17 and CaETR4 in marker-assisted selection. These findings underline the complexity of polygenic resistance to A. rabiei and emphasize the importance of integrating phenotypic screening with genetic analyses to improve the reliability of chickpea breeding programs. This work also contributes to identifying superior MR genotypes, providing valuable resources for the development of resistant cultivars.Instituto de Patología VegetalFil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Castro, Patricia. Universidad de Córdoba (UCO); EspañaFil: Millán, Teresa. Universidad de Córdoba (UCO); EspañaFil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Pergamino; ArgentinaFil: Iglesias, Juliana.Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA), Pergamino; ArgentinaFil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaSpringer2025-07-15T10:19:59Z2025-07-15T10:19:59Z2025-05-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23020https://link.springer.com/article/10.1007/s10681-025-03557-w0014-23361573-5060 (online)https://doi.org/10.1007/s10681-025-03557-wEuphytica 221 : 108 (June 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E6-I132-001, Desarrollo de legumbres adaptadas a los territorios y diferenciados por calidad comercial, nutricional e industrialinfo:eu-repo/semantics/reference/hdl/20.500.12123/21430info:eu-repo/semantics/reference/hdl/20.500.12123/21433info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:11Zoai:localhost:20.500.12123/23020instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:11.615INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Unlocking hidden sources of resistance to ascochyta blight in moderately resistant chickpea genotypes
title Unlocking hidden sources of resistance to ascochyta blight in moderately resistant chickpea genotypes
spellingShingle Unlocking hidden sources of resistance to ascochyta blight in moderately resistant chickpea genotypes
Crociara, Clara Sonia
Ascochyta rabiei
Garbanzo
Tizón del garbanzo
Chickpea blight
Mejoramiento Genético
Chickpeas
Genetic Improvement
Molecular Markers
Pulse Breeding
Rabia del Garbanzo
title_short Unlocking hidden sources of resistance to ascochyta blight in moderately resistant chickpea genotypes
title_full Unlocking hidden sources of resistance to ascochyta blight in moderately resistant chickpea genotypes
title_fullStr Unlocking hidden sources of resistance to ascochyta blight in moderately resistant chickpea genotypes
title_full_unstemmed Unlocking hidden sources of resistance to ascochyta blight in moderately resistant chickpea genotypes
title_sort Unlocking hidden sources of resistance to ascochyta blight in moderately resistant chickpea genotypes
dc.creator.none.fl_str_mv Crociara, Clara Sonia
Valetti, Lucio
Castro, Patricia
Millán, Teresa
Iglesias, Juliana
Pastor, Silvina Estela
author Crociara, Clara Sonia
author_facet Crociara, Clara Sonia
Valetti, Lucio
Castro, Patricia
Millán, Teresa
Iglesias, Juliana
Pastor, Silvina Estela
author_role author
author2 Valetti, Lucio
Castro, Patricia
Millán, Teresa
Iglesias, Juliana
Pastor, Silvina Estela
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ascochyta rabiei
Garbanzo
Tizón del garbanzo
Chickpea blight
Mejoramiento Genético
Chickpeas
Genetic Improvement
Molecular Markers
Pulse Breeding
Rabia del Garbanzo
topic Ascochyta rabiei
Garbanzo
Tizón del garbanzo
Chickpea blight
Mejoramiento Genético
Chickpeas
Genetic Improvement
Molecular Markers
Pulse Breeding
Rabia del Garbanzo
dc.description.none.fl_txt_mv Ascochyta blight, caused by Ascochyta rabiei, is a major threat to global chickpea (Cicer arietinum L.) production, significantly reducing yield under favorable conditions. This study aimed to characterize the resistance responses of nine chickpea genotypes, previously classified as moderately resistant, by subjecting them to enhanced disease pressure. Phenotypic evaluation, including the area under the disease progress curve (AUDPC) and severity scoring was carried out. To investigate the potential genetic basis of the observed resistance response, we analyzed molecular markers previously reported as linked to quantitative trait loci (QTLs) for A. rabiei resistance. The approach focused on testing whether these established markers could distinguish genotypes following the same patterns observed in their phenotypic responses under increased pathogen pressure. The results revealed significant variability among the MR genotypes, with three genotypes FLIP06-86C, FLIP07-35C, and FLIP03-100C outperforming the resistant control. The results from hierarchical clustering (UPGMA), principal component analysis (PCA), and principal coordinate analysis (PCoA) highlighted genetic substructures consistent with observed phenotypic behaviors. However, unexpected marker-phenotype associations were detected, questioning the utility of specific markers such as SCY17 and CaETR4 in marker-assisted selection. These findings underline the complexity of polygenic resistance to A. rabiei and emphasize the importance of integrating phenotypic screening with genetic analyses to improve the reliability of chickpea breeding programs. This work also contributes to identifying superior MR genotypes, providing valuable resources for the development of resistant cultivars.
Instituto de Patología Vegetal
Fil: Crociara, Clara Sonia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Crociara, Clara Sonia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Valetti, Lucio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Valetti, Lucio. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Castro, Patricia. Universidad de Córdoba (UCO); España
Fil: Millán, Teresa. Universidad de Córdoba (UCO); España
Fil: Iglesias, Juliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Pergamino; Argentina
Fil: Iglesias, Juliana.Universidad Nacional del Noroeste de la Provincia de Buenos Aires (UNNOBA), Pergamino; Argentina
Fil: Pastor, Silvina Estela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Pastor, Silvina Estela. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
description Ascochyta blight, caused by Ascochyta rabiei, is a major threat to global chickpea (Cicer arietinum L.) production, significantly reducing yield under favorable conditions. This study aimed to characterize the resistance responses of nine chickpea genotypes, previously classified as moderately resistant, by subjecting them to enhanced disease pressure. Phenotypic evaluation, including the area under the disease progress curve (AUDPC) and severity scoring was carried out. To investigate the potential genetic basis of the observed resistance response, we analyzed molecular markers previously reported as linked to quantitative trait loci (QTLs) for A. rabiei resistance. The approach focused on testing whether these established markers could distinguish genotypes following the same patterns observed in their phenotypic responses under increased pathogen pressure. The results revealed significant variability among the MR genotypes, with three genotypes FLIP06-86C, FLIP07-35C, and FLIP03-100C outperforming the resistant control. The results from hierarchical clustering (UPGMA), principal component analysis (PCA), and principal coordinate analysis (PCoA) highlighted genetic substructures consistent with observed phenotypic behaviors. However, unexpected marker-phenotype associations were detected, questioning the utility of specific markers such as SCY17 and CaETR4 in marker-assisted selection. These findings underline the complexity of polygenic resistance to A. rabiei and emphasize the importance of integrating phenotypic screening with genetic analyses to improve the reliability of chickpea breeding programs. This work also contributes to identifying superior MR genotypes, providing valuable resources for the development of resistant cultivars.
publishDate 2025
dc.date.none.fl_str_mv 2025-07-15T10:19:59Z
2025-07-15T10:19:59Z
2025-05-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23020
https://link.springer.com/article/10.1007/s10681-025-03557-w
0014-2336
1573-5060 (online)
https://doi.org/10.1007/s10681-025-03557-w
url http://hdl.handle.net/20.500.12123/23020
https://link.springer.com/article/10.1007/s10681-025-03557-w
https://doi.org/10.1007/s10681-025-03557-w
identifier_str_mv 0014-2336
1573-5060 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E6-I132-001, Desarrollo de legumbres adaptadas a los territorios y diferenciados por calidad comercial, nutricional e industrial
info:eu-repo/semantics/reference/hdl/20.500.12123/21430
info:eu-repo/semantics/reference/hdl/20.500.12123/21433
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Euphytica 221 : 108 (June 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341442665578496
score 12.623145

Publicaciones similares