Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis

Autores
Griffa, Natanael; Moyano, Roberto Damián; Canal, Ana María; Traveria, Gabriel Eduardo; Santangelo, María De La Paz; Alonso, Maria Natalia; Romano, Maria Isabel
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving the efficiency of the TST has become a critical issue. The detection of Mycobacterium bovis antibodies in serum, within weeks after the TST, may be a rapid and inexpensive way to improve bTB control. This study reports the validation of an enzyme-linked immunosorbent assay (ELISA) to detect bovine tuberculosis as an ancillary test to TST in dairy farms in Argentina. The estimated validation parameters were within the established requirements of the World Organization for Animal Health (OIE). The test demonstrated high repeatability, with coefficients of variation <25%. High test reproducibility through interlaboratory testing was also found, with an estimated Pearson coefficient of 0.9648 (95% confidence intervals 0.9315–0.9820). The ELISA detected tuberculous cattle unidentified by the TST. Of 43 animals sent to slaughterhouses that were ELISA positive 15–17 days after a negative TST, 36 were confirmed as infected with M. bovis by histopathology and IS6110 PCR. According to ROC curve analysis of results of 145 cattle from M. bovis-free herds and the 36 M. bovis-infected cattle, at a corrected optical density cut-off point of 0.3853, specificity was 95.95% and the positive predictive value at this cut-off was 83.72%. The ELISA detection test validated in this study could be readily applied in dairy farms, to complement a prior TST and improve livestock health.
Instituto de Biotecnologìa
Fil: Griffa Natanael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Traveria, Gabriel Eduardo. Universidad de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
The Veterinary Journal 256 : 105426 (Febrero 2020)
Materia
Tuberculosis
Infeccion por Mycobacterium bovis
ELISA
Mycobacterium bovis Infections
Tests
Humoral Response
Respuesta Humoral
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7787
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7787
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spelling Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosisGriffa, NatanaelMoyano, Roberto DamiánCanal, Ana MaríaTraveria, Gabriel EduardoSantangelo, María De La PazAlonso, Maria NataliaRomano, Maria IsabelTuberculosisInfeccion por Mycobacterium bovisELISAMycobacterium bovis InfectionsTestsHumoral ResponseRespuesta HumoralPrograms for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving the efficiency of the TST has become a critical issue. The detection of Mycobacterium bovis antibodies in serum, within weeks after the TST, may be a rapid and inexpensive way to improve bTB control. This study reports the validation of an enzyme-linked immunosorbent assay (ELISA) to detect bovine tuberculosis as an ancillary test to TST in dairy farms in Argentina. The estimated validation parameters were within the established requirements of the World Organization for Animal Health (OIE). The test demonstrated high repeatability, with coefficients of variation <25%. High test reproducibility through interlaboratory testing was also found, with an estimated Pearson coefficient of 0.9648 (95% confidence intervals 0.9315–0.9820). The ELISA detected tuberculous cattle unidentified by the TST. Of 43 animals sent to slaughterhouses that were ELISA positive 15–17 days after a negative TST, 36 were confirmed as infected with M. bovis by histopathology and IS6110 PCR. According to ROC curve analysis of results of 145 cattle from M. bovis-free herds and the 36 M. bovis-infected cattle, at a corrected optical density cut-off point of 0.3853, specificity was 95.95% and the positive predictive value at this cut-off was 83.72%. The ELISA detection test validated in this study could be readily applied in dairy farms, to complement a prior TST and improve livestock health.Instituto de BiotecnologìaFil: Griffa Natanael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Traveria, Gabriel Eduardo. Universidad de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; ArgentinaFil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alonso Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2020-08-28T16:46:14Z2020-08-28T16:46:14Z2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7787https://www.sciencedirect.com/science/article/abs/pii/S10900233203000341090-0233https://doi.org/10.1016/j.tvjl.2020.105426The Veterinary Journal 256 : 105426 (Febrero 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:52Zoai:localhost:20.500.12123/7787instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:53.135INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
title Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
spellingShingle Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
Griffa, Natanael
Tuberculosis
Infeccion por Mycobacterium bovis
ELISA
Mycobacterium bovis Infections
Tests
Humoral Response
Respuesta Humoral
title_short Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
title_full Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
title_fullStr Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
title_full_unstemmed Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
title_sort Development and diagnostic validation of an ELISA based on an antigenic mixture for the detection of bovine tuberculosis
dc.creator.none.fl_str_mv Griffa, Natanael
Moyano, Roberto Damián
Canal, Ana María
Traveria, Gabriel Eduardo
Santangelo, María De La Paz
Alonso, Maria Natalia
Romano, Maria Isabel
author Griffa, Natanael
author_facet Griffa, Natanael
Moyano, Roberto Damián
Canal, Ana María
Traveria, Gabriel Eduardo
Santangelo, María De La Paz
Alonso, Maria Natalia
Romano, Maria Isabel
author_role author
author2 Moyano, Roberto Damián
Canal, Ana María
Traveria, Gabriel Eduardo
Santangelo, María De La Paz
Alonso, Maria Natalia
Romano, Maria Isabel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Tuberculosis
Infeccion por Mycobacterium bovis
ELISA
Mycobacterium bovis Infections
Tests
Humoral Response
Respuesta Humoral
topic Tuberculosis
Infeccion por Mycobacterium bovis
ELISA
Mycobacterium bovis Infections
Tests
Humoral Response
Respuesta Humoral
dc.description.none.fl_txt_mv Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving the efficiency of the TST has become a critical issue. The detection of Mycobacterium bovis antibodies in serum, within weeks after the TST, may be a rapid and inexpensive way to improve bTB control. This study reports the validation of an enzyme-linked immunosorbent assay (ELISA) to detect bovine tuberculosis as an ancillary test to TST in dairy farms in Argentina. The estimated validation parameters were within the established requirements of the World Organization for Animal Health (OIE). The test demonstrated high repeatability, with coefficients of variation <25%. High test reproducibility through interlaboratory testing was also found, with an estimated Pearson coefficient of 0.9648 (95% confidence intervals 0.9315–0.9820). The ELISA detected tuberculous cattle unidentified by the TST. Of 43 animals sent to slaughterhouses that were ELISA positive 15–17 days after a negative TST, 36 were confirmed as infected with M. bovis by histopathology and IS6110 PCR. According to ROC curve analysis of results of 145 cattle from M. bovis-free herds and the 36 M. bovis-infected cattle, at a corrected optical density cut-off point of 0.3853, specificity was 95.95% and the positive predictive value at this cut-off was 83.72%. The ELISA detection test validated in this study could be readily applied in dairy farms, to complement a prior TST and improve livestock health.
Instituto de Biotecnologìa
Fil: Griffa Natanael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Traveria, Gabriel Eduardo. Universidad de La Plata. Facultad de Ciencias Veterinarias. Centro de Diagnóstico e Investigaciones Veterinarias; Argentina
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alonso Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Programs for the eradication of bovine tuberculosis (bTB) focus on the tuberculin skin test (TST) and slaughter of reactor cattle. However, the disease remains an animal health concern in several countries and improving the efficiency of the TST has become a critical issue. The detection of Mycobacterium bovis antibodies in serum, within weeks after the TST, may be a rapid and inexpensive way to improve bTB control. This study reports the validation of an enzyme-linked immunosorbent assay (ELISA) to detect bovine tuberculosis as an ancillary test to TST in dairy farms in Argentina. The estimated validation parameters were within the established requirements of the World Organization for Animal Health (OIE). The test demonstrated high repeatability, with coefficients of variation <25%. High test reproducibility through interlaboratory testing was also found, with an estimated Pearson coefficient of 0.9648 (95% confidence intervals 0.9315–0.9820). The ELISA detected tuberculous cattle unidentified by the TST. Of 43 animals sent to slaughterhouses that were ELISA positive 15–17 days after a negative TST, 36 were confirmed as infected with M. bovis by histopathology and IS6110 PCR. According to ROC curve analysis of results of 145 cattle from M. bovis-free herds and the 36 M. bovis-infected cattle, at a corrected optical density cut-off point of 0.3853, specificity was 95.95% and the positive predictive value at this cut-off was 83.72%. The ELISA detection test validated in this study could be readily applied in dairy farms, to complement a prior TST and improve livestock health.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-28T16:46:14Z
2020-08-28T16:46:14Z
2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7787
https://www.sciencedirect.com/science/article/abs/pii/S1090023320300034
1090-0233
https://doi.org/10.1016/j.tvjl.2020.105426
url http://hdl.handle.net/20.500.12123/7787
https://www.sciencedirect.com/science/article/abs/pii/S1090023320300034
https://doi.org/10.1016/j.tvjl.2020.105426
identifier_str_mv 1090-0233
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv The Veterinary Journal 256 : 105426 (Febrero 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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