Role of PhoPR in the response to stress of Mycobacterium bovis

Autores
Garcia, Elizabeth Andrea; Blanco, Federico Carlos; Klepp, Laura Ines; Pazos, Adriana Alejandra; McNeil, Michael; Jackson, Mary; Bigi, Fabiana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
PhoP is part of the two-component PhoPR system that regulates the expression of virulence genes of Mycobacteria. The goal of this work was to elucidate the role of PhoP in the mechanism that Mycobacterium bovis, the causative agent of bovine tuberculosis, displays upon stress. An analysis of gene expression and acidic growth curves indicated that M. bovis neutralized the external acidic environment by inducing and secreting ammonia. We found that PhoP is essential for ammonia production/secretion and its role in this process seems to be the induction of asparaginase and urease expression. We also demonstrated that the lack of PhoP negatively affected the synthesis of phthiocerol dimycocerosates. This finding is consistent with the role of the lipid anabolism in maintaining the redox environment upon stress in mycobacteria. Altogether the results of this study indicate that PhoP plays an important role in the response mechanisms to stress of M. bovis.
Instituto de Biotecnología
Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pazos, Adriana Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina
Fil: McNeil, Michael. Colorado State University. Department of Microbiology, Immunology and Pathology; Estados Unidos
Fil: Jackson, Mary. Colorado State University. Department of Microbiology, Immunology and Pathology; Estados Unidos
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Comparative Immunology, Microbiology and Infectious Diseases 74 : 101593 (Febrero 2021)
Materia
Mycobacterium bovis
Estres
Virulencia
Infeccion por Mycobacterium bovis
Amoníaco
Stress
Virulence
Mycobacterium bovis Infections
Tuberculosis
Ammonia
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9089
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oai_identifier_str oai:localhost:20.500.12123/9089
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spelling Role of PhoPR in the response to stress of Mycobacterium bovisGarcia, Elizabeth AndreaBlanco, Federico CarlosKlepp, Laura InesPazos, Adriana AlejandraMcNeil, MichaelJackson, MaryBigi, FabianaMycobacterium bovisEstresVirulenciaInfeccion por Mycobacterium bovisAmoníacoStressVirulenceMycobacterium bovis InfectionsTuberculosisAmmoniaPhoP is part of the two-component PhoPR system that regulates the expression of virulence genes of Mycobacteria. The goal of this work was to elucidate the role of PhoP in the mechanism that Mycobacterium bovis, the causative agent of bovine tuberculosis, displays upon stress. An analysis of gene expression and acidic growth curves indicated that M. bovis neutralized the external acidic environment by inducing and secreting ammonia. We found that PhoP is essential for ammonia production/secretion and its role in this process seems to be the induction of asparaginase and urease expression. We also demonstrated that the lack of PhoP negatively affected the synthesis of phthiocerol dimycocerosates. This finding is consistent with the role of the lipid anabolism in maintaining the redox environment upon stress in mycobacteria. Altogether the results of this study indicate that PhoP plays an important role in the response mechanisms to stress of M. bovis.Instituto de BiotecnologíaFil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pazos, Adriana Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; ArgentinaFil: McNeil, Michael. Colorado State University. Department of Microbiology, Immunology and Pathology; Estados UnidosFil: Jackson, Mary. Colorado State University. Department of Microbiology, Immunology and Pathology; Estados UnidosFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2021-04-14T16:33:03Z2021-04-14T16:33:03Z2021-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9089https://www.sciencedirect.com/science/article/abs/pii/S014795712030182X1878-1667https://doi.org/10.1016/j.cimid.2020.101593Comparative Immunology, Microbiology and Infectious Diseases 74 : 101593 (Febrero 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud”info:eu-repo/semantics/restrictedAccess2025-09-04T09:48:50Zoai:localhost:20.500.12123/9089instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:51.303INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Role of PhoPR in the response to stress of Mycobacterium bovis
title Role of PhoPR in the response to stress of Mycobacterium bovis
spellingShingle Role of PhoPR in the response to stress of Mycobacterium bovis
Garcia, Elizabeth Andrea
Mycobacterium bovis
Estres
Virulencia
Infeccion por Mycobacterium bovis
Amoníaco
Stress
Virulence
Mycobacterium bovis Infections
Tuberculosis
Ammonia
title_short Role of PhoPR in the response to stress of Mycobacterium bovis
title_full Role of PhoPR in the response to stress of Mycobacterium bovis
title_fullStr Role of PhoPR in the response to stress of Mycobacterium bovis
title_full_unstemmed Role of PhoPR in the response to stress of Mycobacterium bovis
title_sort Role of PhoPR in the response to stress of Mycobacterium bovis
dc.creator.none.fl_str_mv Garcia, Elizabeth Andrea
Blanco, Federico Carlos
Klepp, Laura Ines
Pazos, Adriana Alejandra
McNeil, Michael
Jackson, Mary
Bigi, Fabiana
author Garcia, Elizabeth Andrea
author_facet Garcia, Elizabeth Andrea
Blanco, Federico Carlos
Klepp, Laura Ines
Pazos, Adriana Alejandra
McNeil, Michael
Jackson, Mary
Bigi, Fabiana
author_role author
author2 Blanco, Federico Carlos
Klepp, Laura Ines
Pazos, Adriana Alejandra
McNeil, Michael
Jackson, Mary
Bigi, Fabiana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium bovis
Estres
Virulencia
Infeccion por Mycobacterium bovis
Amoníaco
Stress
Virulence
Mycobacterium bovis Infections
Tuberculosis
Ammonia
topic Mycobacterium bovis
Estres
Virulencia
Infeccion por Mycobacterium bovis
Amoníaco
Stress
Virulence
Mycobacterium bovis Infections
Tuberculosis
Ammonia
dc.description.none.fl_txt_mv PhoP is part of the two-component PhoPR system that regulates the expression of virulence genes of Mycobacteria. The goal of this work was to elucidate the role of PhoP in the mechanism that Mycobacterium bovis, the causative agent of bovine tuberculosis, displays upon stress. An analysis of gene expression and acidic growth curves indicated that M. bovis neutralized the external acidic environment by inducing and secreting ammonia. We found that PhoP is essential for ammonia production/secretion and its role in this process seems to be the induction of asparaginase and urease expression. We also demonstrated that the lack of PhoP negatively affected the synthesis of phthiocerol dimycocerosates. This finding is consistent with the role of the lipid anabolism in maintaining the redox environment upon stress in mycobacteria. Altogether the results of this study indicate that PhoP plays an important role in the response mechanisms to stress of M. bovis.
Instituto de Biotecnología
Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Garcia, Elizabeth Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Blanco, Federico Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Klepp, Laura Ines. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Klepp, Laura Ines. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pazos, Adriana Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto Tecnología de Alimentos; Argentina
Fil: McNeil, Michael. Colorado State University. Department of Microbiology, Immunology and Pathology; Estados Unidos
Fil: Jackson, Mary. Colorado State University. Department of Microbiology, Immunology and Pathology; Estados Unidos
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Bigi, Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description PhoP is part of the two-component PhoPR system that regulates the expression of virulence genes of Mycobacteria. The goal of this work was to elucidate the role of PhoP in the mechanism that Mycobacterium bovis, the causative agent of bovine tuberculosis, displays upon stress. An analysis of gene expression and acidic growth curves indicated that M. bovis neutralized the external acidic environment by inducing and secreting ammonia. We found that PhoP is essential for ammonia production/secretion and its role in this process seems to be the induction of asparaginase and urease expression. We also demonstrated that the lack of PhoP negatively affected the synthesis of phthiocerol dimycocerosates. This finding is consistent with the role of the lipid anabolism in maintaining the redox environment upon stress in mycobacteria. Altogether the results of this study indicate that PhoP plays an important role in the response mechanisms to stress of M. bovis.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-14T16:33:03Z
2021-04-14T16:33:03Z
2021-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9089
https://www.sciencedirect.com/science/article/abs/pii/S014795712030182X
1878-1667
https://doi.org/10.1016/j.cimid.2020.101593
url http://hdl.handle.net/20.500.12123/9089
https://www.sciencedirect.com/science/article/abs/pii/S014795712030182X
https://doi.org/10.1016/j.cimid.2020.101593
identifier_str_mv 1878-1667
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I105-001/2019-PD-E5-I105-001/AR./Patógenos animales: su interacción con el hospedador y el medio ambiente. Impacto en productividad, ecosistemas, sanidad animal y salud pública en el marco “Una Salud”
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Comparative Immunology, Microbiology and Infectious Diseases 74 : 101593 (Febrero 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1842341386327687168
score 12.623145

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