Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis

Autores
Alonso, Maria Natalia; Griffa, Natanael; Moyano, Roberto Damián; Mon, Maria Laura; Colombatti Olivieri, Maria Alejandra; Barandiaran, Soledad; Martinez Vivot, Marcela; Fiorini, Gonzalo; Canal, Ana María; Santangelo, María De La Paz; Singh, Mahavir G.; Romano, Maria Isabel
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Detection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as a complement to the single skin test. We developed a lateral flow immunochromatography (LFIC) test for rapid bTB detection based on the use of a conjugate of gold nanoparticles with a recombinant G protein. After evaluating 3 Mycobacterium bovis (MB) antigens: ESAT-6, CFP-10 and MPB83 for the control line, we selected MPB83 given it was the most specific. The performance of the test was analyzed with 820 bovine sera, 40 sera corresponding to healthy animals, 5 sera from animals infected with Mycobacterium avium subsp. paratuberculosis (MAP) and 775 sera of animals from herds with bTB. All these sera were also submitted to a validated bTB-ELISA using whole-cell antigen from MB. From the 775 sera of animals from herds with bTB, 87 sera were positive by the bTB-ELISA, 45 were positive by LFIC and only 5 animals were positives by skin test (TST). To confirm bTB infection in the group of TST (−), bTB-ELISA (+) and LFIC (+) animals, we performed postmortem examination in 15 randomly selected animals. Macroscopically, these 15 animals had numerous small and large yellow-white granulomas, characteristic of bTB, and the infection was subsequently confirmed by PCR in these tissues with lesions (gold standard). No false positive test result was detected with the developed LFIC either with the sera from healthy animals or from animals infected with MAP demonstrating that it can be a useful technique for the rapid identification of animals infected with bTB.
Instituto de Biotecnología
Fil: Alonso Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Alonso Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Griffa Natanael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Griffa Natanael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Martinez Vivot, Marcela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Fiorini, Gonzalo. General Pinto; Argentina
Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Singh, Mahavir G. Lionnex Diagnostics & Therapeutics GmbH; Alemania
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of Immunological Methods 491 : 112941 (Abril 2021)
Materia
Immunological Techniques
Mycobacterium bovis Infections
Chromatography
Tests
Antibodies
Técnicas Inmunológicas
Mycobacterium bovis
Tuberculosis
Infeccion por Mycobacterium bovis
Bovinae
Cromatografía
Anticuerpos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9147
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9147
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network_name_str INTA Digital (INTA)
spelling Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosisAlonso, Maria NataliaGriffa, NatanaelMoyano, Roberto DamiánMon, Maria LauraColombatti Olivieri, Maria AlejandraBarandiaran, SoledadMartinez Vivot, MarcelaFiorini, GonzaloCanal, Ana MaríaSantangelo, María De La PazSingh, Mahavir G.Romano, Maria IsabelImmunological TechniquesMycobacterium bovis InfectionsChromatographyTestsAntibodiesTécnicas InmunológicasMycobacterium bovisTuberculosisInfeccion por Mycobacterium bovisBovinaeCromatografíaAnticuerposDetection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as a complement to the single skin test. We developed a lateral flow immunochromatography (LFIC) test for rapid bTB detection based on the use of a conjugate of gold nanoparticles with a recombinant G protein. After evaluating 3 Mycobacterium bovis (MB) antigens: ESAT-6, CFP-10 and MPB83 for the control line, we selected MPB83 given it was the most specific. The performance of the test was analyzed with 820 bovine sera, 40 sera corresponding to healthy animals, 5 sera from animals infected with Mycobacterium avium subsp. paratuberculosis (MAP) and 775 sera of animals from herds with bTB. All these sera were also submitted to a validated bTB-ELISA using whole-cell antigen from MB. From the 775 sera of animals from herds with bTB, 87 sera were positive by the bTB-ELISA, 45 were positive by LFIC and only 5 animals were positives by skin test (TST). To confirm bTB infection in the group of TST (−), bTB-ELISA (+) and LFIC (+) animals, we performed postmortem examination in 15 randomly selected animals. Macroscopically, these 15 animals had numerous small and large yellow-white granulomas, characteristic of bTB, and the infection was subsequently confirmed by PCR in these tissues with lesions (gold standard). No false positive test result was detected with the developed LFIC either with the sera from healthy animals or from animals infected with MAP demonstrating that it can be a useful technique for the rapid identification of animals infected with bTB.Instituto de BiotecnologíaFil: Alonso Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Alonso Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Griffa Natanael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Griffa Natanael. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Martinez Vivot, Marcela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Fiorini, Gonzalo. General Pinto; ArgentinaFil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Singh, Mahavir G. Lionnex Diagnostics & Therapeutics GmbH; AlemaniaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2021-04-21T15:54:09Z2021-04-21T15:54:09Z2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9147https://www.sciencedirect.com/science/article/pii/S00221759203023501872-7905https://doi.org/10.1016/j.jim.2020.112941Journal of Immunological Methods 491 : 112941 (Abril 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:11Zoai:localhost:20.500.12123/9147instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:11.872INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
title Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
spellingShingle Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
Alonso, Maria Natalia
Immunological Techniques
Mycobacterium bovis Infections
Chromatography
Tests
Antibodies
Técnicas Inmunológicas
Mycobacterium bovis
Tuberculosis
Infeccion por Mycobacterium bovis
Bovinae
Cromatografía
Anticuerpos
title_short Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
title_full Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
title_fullStr Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
title_full_unstemmed Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
title_sort Development of a lateral flow immunochromatography test for the rapid detection of bovine tuberculosis
dc.creator.none.fl_str_mv Alonso, Maria Natalia
Griffa, Natanael
Moyano, Roberto Damián
Mon, Maria Laura
Colombatti Olivieri, Maria Alejandra
Barandiaran, Soledad
Martinez Vivot, Marcela
Fiorini, Gonzalo
Canal, Ana María
Santangelo, María De La Paz
Singh, Mahavir G.
Romano, Maria Isabel
author Alonso, Maria Natalia
author_facet Alonso, Maria Natalia
Griffa, Natanael
Moyano, Roberto Damián
Mon, Maria Laura
Colombatti Olivieri, Maria Alejandra
Barandiaran, Soledad
Martinez Vivot, Marcela
Fiorini, Gonzalo
Canal, Ana María
Santangelo, María De La Paz
Singh, Mahavir G.
Romano, Maria Isabel
author_role author
author2 Griffa, Natanael
Moyano, Roberto Damián
Mon, Maria Laura
Colombatti Olivieri, Maria Alejandra
Barandiaran, Soledad
Martinez Vivot, Marcela
Fiorini, Gonzalo
Canal, Ana María
Santangelo, María De La Paz
Singh, Mahavir G.
Romano, Maria Isabel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Immunological Techniques
Mycobacterium bovis Infections
Chromatography
Tests
Antibodies
Técnicas Inmunológicas
Mycobacterium bovis
Tuberculosis
Infeccion por Mycobacterium bovis
Bovinae
Cromatografía
Anticuerpos
topic Immunological Techniques
Mycobacterium bovis Infections
Chromatography
Tests
Antibodies
Técnicas Inmunológicas
Mycobacterium bovis
Tuberculosis
Infeccion por Mycobacterium bovis
Bovinae
Cromatografía
Anticuerpos
dc.description.none.fl_txt_mv Detection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as a complement to the single skin test. We developed a lateral flow immunochromatography (LFIC) test for rapid bTB detection based on the use of a conjugate of gold nanoparticles with a recombinant G protein. After evaluating 3 Mycobacterium bovis (MB) antigens: ESAT-6, CFP-10 and MPB83 for the control line, we selected MPB83 given it was the most specific. The performance of the test was analyzed with 820 bovine sera, 40 sera corresponding to healthy animals, 5 sera from animals infected with Mycobacterium avium subsp. paratuberculosis (MAP) and 775 sera of animals from herds with bTB. All these sera were also submitted to a validated bTB-ELISA using whole-cell antigen from MB. From the 775 sera of animals from herds with bTB, 87 sera were positive by the bTB-ELISA, 45 were positive by LFIC and only 5 animals were positives by skin test (TST). To confirm bTB infection in the group of TST (−), bTB-ELISA (+) and LFIC (+) animals, we performed postmortem examination in 15 randomly selected animals. Macroscopically, these 15 animals had numerous small and large yellow-white granulomas, characteristic of bTB, and the infection was subsequently confirmed by PCR in these tissues with lesions (gold standard). No false positive test result was detected with the developed LFIC either with the sera from healthy animals or from animals infected with MAP demonstrating that it can be a useful technique for the rapid identification of animals infected with bTB.
Instituto de Biotecnología
Fil: Alonso Maria Natalia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Alonso Maria Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Griffa Natanael. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Griffa Natanael. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Moyano, Roberto Damian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Moyano, Roberto Damian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Colombatti Olivieri, Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Martinez Vivot, Marcela. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Fiorini, Gonzalo. General Pinto; Argentina
Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Santangelo, María De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Santangelo, María De La Paz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Singh, Mahavir G. Lionnex Diagnostics & Therapeutics GmbH; Alemania
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Romano, Maria Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Detection of specific antibodies would be a useful test strategy for bovine tuberculosis (bTB) as a complement to the single skin test. We developed a lateral flow immunochromatography (LFIC) test for rapid bTB detection based on the use of a conjugate of gold nanoparticles with a recombinant G protein. After evaluating 3 Mycobacterium bovis (MB) antigens: ESAT-6, CFP-10 and MPB83 for the control line, we selected MPB83 given it was the most specific. The performance of the test was analyzed with 820 bovine sera, 40 sera corresponding to healthy animals, 5 sera from animals infected with Mycobacterium avium subsp. paratuberculosis (MAP) and 775 sera of animals from herds with bTB. All these sera were also submitted to a validated bTB-ELISA using whole-cell antigen from MB. From the 775 sera of animals from herds with bTB, 87 sera were positive by the bTB-ELISA, 45 were positive by LFIC and only 5 animals were positives by skin test (TST). To confirm bTB infection in the group of TST (−), bTB-ELISA (+) and LFIC (+) animals, we performed postmortem examination in 15 randomly selected animals. Macroscopically, these 15 animals had numerous small and large yellow-white granulomas, characteristic of bTB, and the infection was subsequently confirmed by PCR in these tissues with lesions (gold standard). No false positive test result was detected with the developed LFIC either with the sera from healthy animals or from animals infected with MAP demonstrating that it can be a useful technique for the rapid identification of animals infected with bTB.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-21T15:54:09Z
2021-04-21T15:54:09Z
2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9147
https://www.sciencedirect.com/science/article/pii/S0022175920302350
1872-7905
https://doi.org/10.1016/j.jim.2020.112941
url http://hdl.handle.net/20.500.12123/9147
https://www.sciencedirect.com/science/article/pii/S0022175920302350
https://doi.org/10.1016/j.jim.2020.112941
identifier_str_mv 1872-7905
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Immunological Methods 491 : 112941 (Abril 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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