Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel

Autores
Capella, Virginia; Rivero, Rebeca E.; Liaudat, Ana Cecilia; Ibarra, Luis Exequiel; Roma, Dardo Andrés; Alustiza, Fabrisio Eduardo; Mañas, Fernando; Barbero, César A.; Bosch, Pablo; Rivarola, Claudia R.; Rodríguez, Nancy
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct.
EEA Marcos Juárez
Fil: Capella, Virginia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivero, Rebeca E. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ibarra, Luis E. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentina
Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentina
Fil: Barbero, César A. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivarola, Claudia R. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodríguez, Nancy. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Heliyon 5 (4) : e01474 (April 2019)
Materia
Citotoxicidad
Biotecnología
Células
ADN
Cytotoxicity
Biotechnology
Cells
DNA
Hidrogeles
Bioingeniería
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5020

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oai_identifier_str oai:localhost:20.500.12123/5020
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogelCapella, VirginiaRivero, Rebeca E.Liaudat, Ana CeciliaIbarra, Luis ExequielRoma, Dardo AndrésAlustiza, Fabrisio EduardoMañas, FernandoBarbero, César A.Bosch, PabloRivarola, Claudia R.Rodríguez, NancyCitotoxicidadBiotecnologíaCélulasADNCytotoxicityBiotechnologyCellsDNAHidrogelesBioingenieríaSeveral hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct.EEA Marcos JuárezFil: Capella, Virginia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivero, Rebeca E. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ibarra, Luis E. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; ArgentinaFil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; ArgentinaFil: Barbero, César A. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivarola, Claudia R. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodríguez, Nancy. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-05-02T18:16:52Z2019-05-02T18:16:52Z2019-04-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.heliyon.com/article/e01474/https://www.sciencedirect.com/science/article/pii/S2405844018387383http://hdl.handle.net/20.500.12123/50202405-8440https://doi.org/10.1016/j.heliyon.2019.e01474Heliyon 5 (4) : e01474 (April 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:38Zoai:localhost:20.500.12123/5020instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:39.207INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel
title Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel
spellingShingle Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel
Capella, Virginia
Citotoxicidad
Biotecnología
Células
ADN
Cytotoxicity
Biotechnology
Cells
DNA
Hidrogeles
Bioingeniería
title_short Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel
title_full Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel
title_fullStr Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel
title_full_unstemmed Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel
title_sort Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel
dc.creator.none.fl_str_mv Capella, Virginia
Rivero, Rebeca E.
Liaudat, Ana Cecilia
Ibarra, Luis Exequiel
Roma, Dardo Andrés
Alustiza, Fabrisio Eduardo
Mañas, Fernando
Barbero, César A.
Bosch, Pablo
Rivarola, Claudia R.
Rodríguez, Nancy
author Capella, Virginia
author_facet Capella, Virginia
Rivero, Rebeca E.
Liaudat, Ana Cecilia
Ibarra, Luis Exequiel
Roma, Dardo Andrés
Alustiza, Fabrisio Eduardo
Mañas, Fernando
Barbero, César A.
Bosch, Pablo
Rivarola, Claudia R.
Rodríguez, Nancy
author_role author
author2 Rivero, Rebeca E.
Liaudat, Ana Cecilia
Ibarra, Luis Exequiel
Roma, Dardo Andrés
Alustiza, Fabrisio Eduardo
Mañas, Fernando
Barbero, César A.
Bosch, Pablo
Rivarola, Claudia R.
Rodríguez, Nancy
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Citotoxicidad
Biotecnología
Células
ADN
Cytotoxicity
Biotechnology
Cells
DNA
Hidrogeles
Bioingeniería
topic Citotoxicidad
Biotecnología
Células
ADN
Cytotoxicity
Biotechnology
Cells
DNA
Hidrogeles
Bioingeniería
dc.description.none.fl_txt_mv Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct.
EEA Marcos Juárez
Fil: Capella, Virginia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivero, Rebeca E. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ibarra, Luis E. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentina
Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentina
Fil: Barbero, César A. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivarola, Claudia R. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodríguez, Nancy. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct.
publishDate 2019
dc.date.none.fl_str_mv 2019-05-02T18:16:52Z
2019-05-02T18:16:52Z
2019-04-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.heliyon.com/article/e01474/
https://www.sciencedirect.com/science/article/pii/S2405844018387383
http://hdl.handle.net/20.500.12123/5020
2405-8440
https://doi.org/10.1016/j.heliyon.2019.e01474
url https://www.heliyon.com/article/e01474/
https://www.sciencedirect.com/science/article/pii/S2405844018387383
http://hdl.handle.net/20.500.12123/5020
https://doi.org/10.1016/j.heliyon.2019.e01474
identifier_str_mv 2405-8440
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Heliyon 5 (4) : e01474 (April 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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