Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel
- Autores
- Capella, Virginia; Rivero, Rebeca E.; Liaudat, Ana Cecilia; Ibarra, Luis Exequiel; Roma, Dardo Andrés; Alustiza, Fabrisio Eduardo; Mañas, Fernando; Barbero, César A.; Bosch, Pablo; Rivarola, Claudia R.; Rodríguez, Nancy
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct.
EEA Marcos Juárez
Fil: Capella, Virginia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivero, Rebeca E. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ibarra, Luis E. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentina
Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentina
Fil: Barbero, César A. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rivarola, Claudia R. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodríguez, Nancy. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Heliyon 5 (4) : e01474 (April 2019)
- Materia
-
Citotoxicidad
Biotecnología
Células
ADN
Cytotoxicity
Biotechnology
Cells
DNA
Hidrogeles
Bioingeniería - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/5020
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Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogelCapella, VirginiaRivero, Rebeca E.Liaudat, Ana CeciliaIbarra, Luis ExequielRoma, Dardo AndrésAlustiza, Fabrisio EduardoMañas, FernandoBarbero, César A.Bosch, PabloRivarola, Claudia R.Rodríguez, NancyCitotoxicidadBiotecnologíaCélulasADNCytotoxicityBiotechnologyCellsDNAHidrogelesBioingenieríaSeveral hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct.EEA Marcos JuárezFil: Capella, Virginia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivero, Rebeca E. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ibarra, Luis E. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; ArgentinaFil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; ArgentinaFil: Barbero, César A. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rivarola, Claudia R. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodríguez, Nancy. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-05-02T18:16:52Z2019-05-02T18:16:52Z2019-04-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.heliyon.com/article/e01474/https://www.sciencedirect.com/science/article/pii/S2405844018387383http://hdl.handle.net/20.500.12123/50202405-8440https://doi.org/10.1016/j.heliyon.2019.e01474Heliyon 5 (4) : e01474 (April 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:38Zoai:localhost:20.500.12123/5020instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:39.207INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
title |
Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
spellingShingle |
Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel Capella, Virginia Citotoxicidad Biotecnología Células ADN Cytotoxicity Biotechnology Cells DNA Hidrogeles Bioingeniería |
title_short |
Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
title_full |
Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
title_fullStr |
Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
title_full_unstemmed |
Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
title_sort |
Cytotoxicity and bioadhesive properties of poly-N-isopropylacrylamide hydrogel |
dc.creator.none.fl_str_mv |
Capella, Virginia Rivero, Rebeca E. Liaudat, Ana Cecilia Ibarra, Luis Exequiel Roma, Dardo Andrés Alustiza, Fabrisio Eduardo Mañas, Fernando Barbero, César A. Bosch, Pablo Rivarola, Claudia R. Rodríguez, Nancy |
author |
Capella, Virginia |
author_facet |
Capella, Virginia Rivero, Rebeca E. Liaudat, Ana Cecilia Ibarra, Luis Exequiel Roma, Dardo Andrés Alustiza, Fabrisio Eduardo Mañas, Fernando Barbero, César A. Bosch, Pablo Rivarola, Claudia R. Rodríguez, Nancy |
author_role |
author |
author2 |
Rivero, Rebeca E. Liaudat, Ana Cecilia Ibarra, Luis Exequiel Roma, Dardo Andrés Alustiza, Fabrisio Eduardo Mañas, Fernando Barbero, César A. Bosch, Pablo Rivarola, Claudia R. Rodríguez, Nancy |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Citotoxicidad Biotecnología Células ADN Cytotoxicity Biotechnology Cells DNA Hidrogeles Bioingeniería |
topic |
Citotoxicidad Biotecnología Células ADN Cytotoxicity Biotechnology Cells DNA Hidrogeles Bioingeniería |
dc.description.none.fl_txt_mv |
Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct. EEA Marcos Juárez Fil: Capella, Virginia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivero, Rebeca E. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Liaudat, Ana Cecilia. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Ibarra, Luis E. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina.Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentina Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Departamento de Clínica Animal; Argentina Fil: Barbero, César A. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bosch, Pablo. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rivarola, Claudia R. Universidad Nacional de Río Cuarto. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rodríguez, Nancy. Universidad Nacional de Río Cuarto. Departamento de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Several hydrogel surfaces present properties that simulate the mechanical and physicochemical features of extracellular matrix (ECM), providing a platform that mimic the native cellular milieus. Poly-N-isopropylacrylamide (PNIPAM) hydrogels are receiving attention in biomedical field due to their thermosensibility and soft texture. However, more extensive biocompatibility and cellular interactions studies with cell lines are needed. Therefore, the aim of this study is focus on evaluating the biocompatibility of PNIPAM through cytotoxicity, genotoxicity, and proliferation tests in murine preadipose cells (3T3-L1), human embryonic kidney cells (HEK293) and human carcinoma-derived cells (A549) in presence of hydrogel surfaces. Bioadhesive capacity above PNIPAM surfaces was also analyzed. MTT and neutral red uptake assays shown non-cytotoxic effect of PNIPAM in the studied cell lines. Genotoxicity was evaluated by the single-cell gel electrophoresis assay, where DNA damages were not detected. [3H]-thymidine staining allowed to corroborate that cell proliferation had progressed correctly. Adopted morphologies for each cell line over PNIPAM were similar to cell growing observed on polystyrene, indicating that the surfaces favor the cell attachment during 5 days' culture. The good biocompatibility of PNIPAM surfaces make it an interesting scaffold with clinical potential in tissue regeneration engineering, and a possible adipose and kidney tissue-engineered construct. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-05-02T18:16:52Z 2019-05-02T18:16:52Z 2019-04-09 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://www.heliyon.com/article/e01474/ https://www.sciencedirect.com/science/article/pii/S2405844018387383 http://hdl.handle.net/20.500.12123/5020 2405-8440 https://doi.org/10.1016/j.heliyon.2019.e01474 |
url |
https://www.heliyon.com/article/e01474/ https://www.sciencedirect.com/science/article/pii/S2405844018387383 http://hdl.handle.net/20.500.12123/5020 https://doi.org/10.1016/j.heliyon.2019.e01474 |
identifier_str_mv |
2405-8440 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Heliyon 5 (4) : e01474 (April 2019) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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