The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection

Autores
Caspe, Sergio Gaston; Livingstone, Morag; Frew, David; Aitchison, Kevin; Wattegedera, Sean Ranjan; Entrican, Gary; Palarea-Albaladejo, Javier; McNeilly, Tom Nathan; Milne, Elspeth; Sargison, Neil Donald; Chianini, Francesca; Longbottom, David
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chlamydia abortus is one of the most commonly diagnosed causes of infectious abortion in small ruminants worldwide. Control of the disease (Enzootic Abortion of Ewes or EAE) is achieved using the commercial live, attenuated C. abortus 1B vaccine strain, which can be distinguished from virulent wild-type (wt) strains by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Published studies applying this typing method and whole-genome sequence analyses to cases of EAE in vaccinated and non-vaccinated animals have provided strong evidence that the 1B strain is not attenuated and can infect the placenta causing disease in some ewes. Therefore, the objective of this study was to characterise the lesions found in the placentas of ewes vaccinated with the 1B strain and to compare these to those resulting from a wt infection. A C. abortus-free flock of multiparous adult ewes was vaccinated twice, over three breeding seasons, each before mating, with the commercial C. abortus 1B vaccine strain (Cevac® Chlamydia, Ceva Animal Health Ltd.). In the second lambing season following vaccination, placentas (n = 117) were collected at parturition and analysed by C. abortus-specific real-time quantitative PCR (qPCR). Two placentas, from a single ewe, which gave birth to live twin lambs, were found to be positive by qPCR and viable organisms were recovered and identified as vaccine type (vt) by PCR-RFLP, with no evidence of any wt strain being present. All cotyledons from the vt-infected placentas were analysed by histopathology and immunohistochemistry and compared to those from wt-infected placentas. Both vt-infected placentas showed lesions typical of those found in a wt infection in terms of their severity, distribution, and associated intensity of antigen labelling. These results conclusively demonstrate that the 1B strain can infect the placenta, producing typical EAE placental lesions that are indistinguishable from those found in wt infected animals.
EEA Mercedes
Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Caspe, Sergio Gaston. Moredun Research Institute; Reino Unido
Fil: Caspe, Sergio Gaston. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido
Fil: Livingstone, Morag. Moredun Research Institute; Reino Unido
Fil: Frew, David. Moredun Research Institute; Reino Unido
Fil: Aitchison, Kevin. Moredun Research Institute; Reino Unido
Fil: Wattegedera, Sean Ranjan. Moredun Research Institute; Reino Unido
Fil: Entrican, Gary. Moredun Research Institute; Reino Unido
Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido
Fil: McNeilly, Tom Nathan. Moredun Research Institute; Reino Unido
Fil: Milne, Elspeth. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido
Fil: Sargison, Neil Donald. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fil: Longbottom, David. Moredun Research Institute; Reino Unido
Fuente
PLoS ONE 15 (11) : e0242526. (2020)
Materia
Ganado Bovino
Enfermedades de los Animales
Chlamydia
Chlamydophila abortus
Control de Enfermedades
Vacuna
Placenta
Cattle
Animal Diseases
Diseases Control
Vaccines
Chlamydia abortus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infectionCaspe, Sergio GastonLivingstone, MoragFrew, DavidAitchison, KevinWattegedera, Sean RanjanEntrican, GaryPalarea-Albaladejo, JavierMcNeilly, Tom NathanMilne, ElspethSargison, Neil DonaldChianini, FrancescaLongbottom, DavidGanado BovinoEnfermedades de los AnimalesChlamydiaChlamydophila abortusControl de EnfermedadesVacunaPlacentaCattleAnimal DiseasesDiseases ControlVaccinesChlamydia abortusChlamydia abortus is one of the most commonly diagnosed causes of infectious abortion in small ruminants worldwide. Control of the disease (Enzootic Abortion of Ewes or EAE) is achieved using the commercial live, attenuated C. abortus 1B vaccine strain, which can be distinguished from virulent wild-type (wt) strains by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Published studies applying this typing method and whole-genome sequence analyses to cases of EAE in vaccinated and non-vaccinated animals have provided strong evidence that the 1B strain is not attenuated and can infect the placenta causing disease in some ewes. Therefore, the objective of this study was to characterise the lesions found in the placentas of ewes vaccinated with the 1B strain and to compare these to those resulting from a wt infection. A C. abortus-free flock of multiparous adult ewes was vaccinated twice, over three breeding seasons, each before mating, with the commercial C. abortus 1B vaccine strain (Cevac® Chlamydia, Ceva Animal Health Ltd.). In the second lambing season following vaccination, placentas (n = 117) were collected at parturition and analysed by C. abortus-specific real-time quantitative PCR (qPCR). Two placentas, from a single ewe, which gave birth to live twin lambs, were found to be positive by qPCR and viable organisms were recovered and identified as vaccine type (vt) by PCR-RFLP, with no evidence of any wt strain being present. All cotyledons from the vt-infected placentas were analysed by histopathology and immunohistochemistry and compared to those from wt-infected placentas. Both vt-infected placentas showed lesions typical of those found in a wt infection in terms of their severity, distribution, and associated intensity of antigen labelling. These results conclusively demonstrate that the 1B strain can infect the placenta, producing typical EAE placental lesions that are indistinguishable from those found in wt infected animals.EEA MercedesFil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Caspe, Sergio Gaston. Moredun Research Institute; Reino UnidoFil: Caspe, Sergio Gaston. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino UnidoFil: Livingstone, Morag. Moredun Research Institute; Reino UnidoFil: Frew, David. Moredun Research Institute; Reino UnidoFil: Aitchison, Kevin. Moredun Research Institute; Reino UnidoFil: Wattegedera, Sean Ranjan. Moredun Research Institute; Reino UnidoFil: Entrican, Gary. Moredun Research Institute; Reino UnidoFil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino UnidoFil: McNeilly, Tom Nathan. Moredun Research Institute; Reino UnidoFil: Milne, Elspeth. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino UnidoFil: Sargison, Neil Donald. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino UnidoFil: Chianini, Francesca. Moredun Research Institute; Reino UnidoFil: Longbottom, David. Moredun Research Institute; Reino UnidoPlos One2021-04-07T13:26:09Z2021-04-07T13:26:09Z2020-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9040https://journals.plos.org/plosone/article?id=10.1371/journal.pone.02425261932-6203https://doi.org/10.1371/journal.pone.0242526PLoS ONE 15 (11) : e0242526. (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:10Zoai:localhost:20.500.12123/9040instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:11.084INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection
title The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection
spellingShingle The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection
Caspe, Sergio Gaston
Ganado Bovino
Enfermedades de los Animales
Chlamydia
Chlamydophila abortus
Control de Enfermedades
Vacuna
Placenta
Cattle
Animal Diseases
Diseases Control
Vaccines
Chlamydia abortus
title_short The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection
title_full The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection
title_fullStr The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection
title_full_unstemmed The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection
title_sort The 1B vaccine strain of Chlamydia abortus produces placental pathology indistinguishable from a wild type infection
dc.creator.none.fl_str_mv Caspe, Sergio Gaston
Livingstone, Morag
Frew, David
Aitchison, Kevin
Wattegedera, Sean Ranjan
Entrican, Gary
Palarea-Albaladejo, Javier
McNeilly, Tom Nathan
Milne, Elspeth
Sargison, Neil Donald
Chianini, Francesca
Longbottom, David
author Caspe, Sergio Gaston
author_facet Caspe, Sergio Gaston
Livingstone, Morag
Frew, David
Aitchison, Kevin
Wattegedera, Sean Ranjan
Entrican, Gary
Palarea-Albaladejo, Javier
McNeilly, Tom Nathan
Milne, Elspeth
Sargison, Neil Donald
Chianini, Francesca
Longbottom, David
author_role author
author2 Livingstone, Morag
Frew, David
Aitchison, Kevin
Wattegedera, Sean Ranjan
Entrican, Gary
Palarea-Albaladejo, Javier
McNeilly, Tom Nathan
Milne, Elspeth
Sargison, Neil Donald
Chianini, Francesca
Longbottom, David
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Chlamydia
Chlamydophila abortus
Control de Enfermedades
Vacuna
Placenta
Cattle
Animal Diseases
Diseases Control
Vaccines
Chlamydia abortus
topic Ganado Bovino
Enfermedades de los Animales
Chlamydia
Chlamydophila abortus
Control de Enfermedades
Vacuna
Placenta
Cattle
Animal Diseases
Diseases Control
Vaccines
Chlamydia abortus
dc.description.none.fl_txt_mv Chlamydia abortus is one of the most commonly diagnosed causes of infectious abortion in small ruminants worldwide. Control of the disease (Enzootic Abortion of Ewes or EAE) is achieved using the commercial live, attenuated C. abortus 1B vaccine strain, which can be distinguished from virulent wild-type (wt) strains by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Published studies applying this typing method and whole-genome sequence analyses to cases of EAE in vaccinated and non-vaccinated animals have provided strong evidence that the 1B strain is not attenuated and can infect the placenta causing disease in some ewes. Therefore, the objective of this study was to characterise the lesions found in the placentas of ewes vaccinated with the 1B strain and to compare these to those resulting from a wt infection. A C. abortus-free flock of multiparous adult ewes was vaccinated twice, over three breeding seasons, each before mating, with the commercial C. abortus 1B vaccine strain (Cevac® Chlamydia, Ceva Animal Health Ltd.). In the second lambing season following vaccination, placentas (n = 117) were collected at parturition and analysed by C. abortus-specific real-time quantitative PCR (qPCR). Two placentas, from a single ewe, which gave birth to live twin lambs, were found to be positive by qPCR and viable organisms were recovered and identified as vaccine type (vt) by PCR-RFLP, with no evidence of any wt strain being present. All cotyledons from the vt-infected placentas were analysed by histopathology and immunohistochemistry and compared to those from wt-infected placentas. Both vt-infected placentas showed lesions typical of those found in a wt infection in terms of their severity, distribution, and associated intensity of antigen labelling. These results conclusively demonstrate that the 1B strain can infect the placenta, producing typical EAE placental lesions that are indistinguishable from those found in wt infected animals.
EEA Mercedes
Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Caspe, Sergio Gaston. Moredun Research Institute; Reino Unido
Fil: Caspe, Sergio Gaston. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido
Fil: Livingstone, Morag. Moredun Research Institute; Reino Unido
Fil: Frew, David. Moredun Research Institute; Reino Unido
Fil: Aitchison, Kevin. Moredun Research Institute; Reino Unido
Fil: Wattegedera, Sean Ranjan. Moredun Research Institute; Reino Unido
Fil: Entrican, Gary. Moredun Research Institute; Reino Unido
Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido
Fil: McNeilly, Tom Nathan. Moredun Research Institute; Reino Unido
Fil: Milne, Elspeth. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido
Fil: Sargison, Neil Donald. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fil: Longbottom, David. Moredun Research Institute; Reino Unido
description Chlamydia abortus is one of the most commonly diagnosed causes of infectious abortion in small ruminants worldwide. Control of the disease (Enzootic Abortion of Ewes or EAE) is achieved using the commercial live, attenuated C. abortus 1B vaccine strain, which can be distinguished from virulent wild-type (wt) strains by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis. Published studies applying this typing method and whole-genome sequence analyses to cases of EAE in vaccinated and non-vaccinated animals have provided strong evidence that the 1B strain is not attenuated and can infect the placenta causing disease in some ewes. Therefore, the objective of this study was to characterise the lesions found in the placentas of ewes vaccinated with the 1B strain and to compare these to those resulting from a wt infection. A C. abortus-free flock of multiparous adult ewes was vaccinated twice, over three breeding seasons, each before mating, with the commercial C. abortus 1B vaccine strain (Cevac® Chlamydia, Ceva Animal Health Ltd.). In the second lambing season following vaccination, placentas (n = 117) were collected at parturition and analysed by C. abortus-specific real-time quantitative PCR (qPCR). Two placentas, from a single ewe, which gave birth to live twin lambs, were found to be positive by qPCR and viable organisms were recovered and identified as vaccine type (vt) by PCR-RFLP, with no evidence of any wt strain being present. All cotyledons from the vt-infected placentas were analysed by histopathology and immunohistochemistry and compared to those from wt-infected placentas. Both vt-infected placentas showed lesions typical of those found in a wt infection in terms of their severity, distribution, and associated intensity of antigen labelling. These results conclusively demonstrate that the 1B strain can infect the placenta, producing typical EAE placental lesions that are indistinguishable from those found in wt infected animals.
publishDate 2020
dc.date.none.fl_str_mv 2020-11
2021-04-07T13:26:09Z
2021-04-07T13:26:09Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9040
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242526
1932-6203
https://doi.org/10.1371/journal.pone.0242526
url http://hdl.handle.net/20.500.12123/9040
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0242526
https://doi.org/10.1371/journal.pone.0242526
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv PLoS ONE 15 (11) : e0242526. (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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