The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes

Autores
Caspe, Sergio Gaston; Ewing, David Andrew; Livingstone, Morag; Underwood, Clare; Milne, Elspeth; Sargison, Neil Donald; Wattegedera, Sean Ranjan; Longbottom, David
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The enzootic abortion of ewes, caused by the bacterium Chlamydia abortus (C. abortus), is one of the main causes of abortion in sheep. There are multiple contributory factors, including chlamydial growth, host immune response, and hormonal balance, that result in different pregnancy outcomes, such as abortion, the birth of weak lambs that may die, or healthy lambs. This study aimed to determine the relationship between phenotypical patterns of immune cell infiltration and different pregnancy outcomes in twin-bearing sheep (both lambs born dead; one alive and one dead; both alive) when experimentally infected with C. abortus. Both the sheep uteri and placentae were collected after parturition. All samples were analysed for specific immune cell features, including cell surface antigens and the T-regulatory (Treg) cell-associated transcription factor and cytokines, by immunohistochemistry and in situ hybridisation. Some of these immunological antigens were evaluated in ovine reproductive tissues for the first time. Differential patterns of T helper/Treg cells revealed significant group effects in the placentae. It suggests the potential role that the balance of lymphocyte subsets may play in affecting different pregnancy outcomes in C. abortus-infected sheep. The present study provides novel detailed information about the immune responses observed at the maternofoetal interface in sheep at the time of pre-term abortion or lambing.
EEA Mercedes
Fil: Caspe, Sergio Gaston. Moredun Research Institute; Reino Unido
Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Ewing, David Andrew. Biomathematics & Statistics Scotland; Reino Unido
Fil: Livingstone, Morag. Moredun Research Institute; Reino Unido
Fil: Underwood, Clare. Moredun Research Institute; Reino Unido
Fil: Milne, Elspeth. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido
Fil: Sargison, Neil Donald. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido
Fil: Wattegedera, Sean Ranjan. Moredun Research Institute; Reino Unido
Fil: Longbottom, David. Moredun Research Institute; Reino Unido
Fuente
Pathogens 12 (6) : 846. (June 2023)
Materia
Oveja
Enfermedades de los Animales
Aborto
Respuesta Inmunológica
Ewes
Chlamydophila abortus
Animal Diseases
Abortion
Placenta
Immune Response
Chlamydia abortus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14680
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oai_identifier_str oai:localhost:20.500.12123/14680
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network_name_str INTA Digital (INTA)
spelling The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy OutcomesCaspe, Sergio GastonEwing, David AndrewLivingstone, MoragUnderwood, ClareMilne, ElspethSargison, Neil DonaldWattegedera, Sean RanjanLongbottom, DavidOvejaEnfermedades de los AnimalesAbortoRespuesta InmunológicaEwesChlamydophila abortusAnimal DiseasesAbortionPlacentaImmune ResponseChlamydia abortusThe enzootic abortion of ewes, caused by the bacterium Chlamydia abortus (C. abortus), is one of the main causes of abortion in sheep. There are multiple contributory factors, including chlamydial growth, host immune response, and hormonal balance, that result in different pregnancy outcomes, such as abortion, the birth of weak lambs that may die, or healthy lambs. This study aimed to determine the relationship between phenotypical patterns of immune cell infiltration and different pregnancy outcomes in twin-bearing sheep (both lambs born dead; one alive and one dead; both alive) when experimentally infected with C. abortus. Both the sheep uteri and placentae were collected after parturition. All samples were analysed for specific immune cell features, including cell surface antigens and the T-regulatory (Treg) cell-associated transcription factor and cytokines, by immunohistochemistry and in situ hybridisation. Some of these immunological antigens were evaluated in ovine reproductive tissues for the first time. Differential patterns of T helper/Treg cells revealed significant group effects in the placentae. It suggests the potential role that the balance of lymphocyte subsets may play in affecting different pregnancy outcomes in C. abortus-infected sheep. The present study provides novel detailed information about the immune responses observed at the maternofoetal interface in sheep at the time of pre-term abortion or lambing.EEA MercedesFil: Caspe, Sergio Gaston. Moredun Research Institute; Reino UnidoFil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Ewing, David Andrew. Biomathematics & Statistics Scotland; Reino UnidoFil: Livingstone, Morag. Moredun Research Institute; Reino UnidoFil: Underwood, Clare. Moredun Research Institute; Reino UnidoFil: Milne, Elspeth. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino UnidoFil: Sargison, Neil Donald. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino UnidoFil: Wattegedera, Sean Ranjan. Moredun Research Institute; Reino UnidoFil: Longbottom, David. Moredun Research Institute; Reino UnidoMDPI2023-07-03T11:24:52Z2023-07-03T11:24:52Z2023-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14680https://www.mdpi.com/2076-0817/12/6/8462076-0817https://doi.org/10.3390/pathogens12060846Pathogens 12 (6) : 846. (June 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:18:22Zoai:localhost:20.500.12123/14680instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:18:22.789INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes
title The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes
spellingShingle The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes
Caspe, Sergio Gaston
Oveja
Enfermedades de los Animales
Aborto
Respuesta Inmunológica
Ewes
Chlamydophila abortus
Animal Diseases
Abortion
Placenta
Immune Response
Chlamydia abortus
title_short The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes
title_full The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes
title_fullStr The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes
title_full_unstemmed The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes
title_sort The Immune Response in the Uteri and Placentae of Chlamydia abortus-Infected Ewes and Its Association with Pregnancy Outcomes
dc.creator.none.fl_str_mv Caspe, Sergio Gaston
Ewing, David Andrew
Livingstone, Morag
Underwood, Clare
Milne, Elspeth
Sargison, Neil Donald
Wattegedera, Sean Ranjan
Longbottom, David
author Caspe, Sergio Gaston
author_facet Caspe, Sergio Gaston
Ewing, David Andrew
Livingstone, Morag
Underwood, Clare
Milne, Elspeth
Sargison, Neil Donald
Wattegedera, Sean Ranjan
Longbottom, David
author_role author
author2 Ewing, David Andrew
Livingstone, Morag
Underwood, Clare
Milne, Elspeth
Sargison, Neil Donald
Wattegedera, Sean Ranjan
Longbottom, David
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Oveja
Enfermedades de los Animales
Aborto
Respuesta Inmunológica
Ewes
Chlamydophila abortus
Animal Diseases
Abortion
Placenta
Immune Response
Chlamydia abortus
topic Oveja
Enfermedades de los Animales
Aborto
Respuesta Inmunológica
Ewes
Chlamydophila abortus
Animal Diseases
Abortion
Placenta
Immune Response
Chlamydia abortus
dc.description.none.fl_txt_mv The enzootic abortion of ewes, caused by the bacterium Chlamydia abortus (C. abortus), is one of the main causes of abortion in sheep. There are multiple contributory factors, including chlamydial growth, host immune response, and hormonal balance, that result in different pregnancy outcomes, such as abortion, the birth of weak lambs that may die, or healthy lambs. This study aimed to determine the relationship between phenotypical patterns of immune cell infiltration and different pregnancy outcomes in twin-bearing sheep (both lambs born dead; one alive and one dead; both alive) when experimentally infected with C. abortus. Both the sheep uteri and placentae were collected after parturition. All samples were analysed for specific immune cell features, including cell surface antigens and the T-regulatory (Treg) cell-associated transcription factor and cytokines, by immunohistochemistry and in situ hybridisation. Some of these immunological antigens were evaluated in ovine reproductive tissues for the first time. Differential patterns of T helper/Treg cells revealed significant group effects in the placentae. It suggests the potential role that the balance of lymphocyte subsets may play in affecting different pregnancy outcomes in C. abortus-infected sheep. The present study provides novel detailed information about the immune responses observed at the maternofoetal interface in sheep at the time of pre-term abortion or lambing.
EEA Mercedes
Fil: Caspe, Sergio Gaston. Moredun Research Institute; Reino Unido
Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Ewing, David Andrew. Biomathematics & Statistics Scotland; Reino Unido
Fil: Livingstone, Morag. Moredun Research Institute; Reino Unido
Fil: Underwood, Clare. Moredun Research Institute; Reino Unido
Fil: Milne, Elspeth. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido
Fil: Sargison, Neil Donald. University of Edinburgh. Royal (Dick) School of Veterinary Studies; Reino Unido
Fil: Wattegedera, Sean Ranjan. Moredun Research Institute; Reino Unido
Fil: Longbottom, David. Moredun Research Institute; Reino Unido
description The enzootic abortion of ewes, caused by the bacterium Chlamydia abortus (C. abortus), is one of the main causes of abortion in sheep. There are multiple contributory factors, including chlamydial growth, host immune response, and hormonal balance, that result in different pregnancy outcomes, such as abortion, the birth of weak lambs that may die, or healthy lambs. This study aimed to determine the relationship between phenotypical patterns of immune cell infiltration and different pregnancy outcomes in twin-bearing sheep (both lambs born dead; one alive and one dead; both alive) when experimentally infected with C. abortus. Both the sheep uteri and placentae were collected after parturition. All samples were analysed for specific immune cell features, including cell surface antigens and the T-regulatory (Treg) cell-associated transcription factor and cytokines, by immunohistochemistry and in situ hybridisation. Some of these immunological antigens were evaluated in ovine reproductive tissues for the first time. Differential patterns of T helper/Treg cells revealed significant group effects in the placentae. It suggests the potential role that the balance of lymphocyte subsets may play in affecting different pregnancy outcomes in C. abortus-infected sheep. The present study provides novel detailed information about the immune responses observed at the maternofoetal interface in sheep at the time of pre-term abortion or lambing.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-03T11:24:52Z
2023-07-03T11:24:52Z
2023-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14680
https://www.mdpi.com/2076-0817/12/6/846
2076-0817
https://doi.org/10.3390/pathogens12060846
url http://hdl.handle.net/20.500.12123/14680
https://www.mdpi.com/2076-0817/12/6/846
https://doi.org/10.3390/pathogens12060846
identifier_str_mv 2076-0817
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Pathogens 12 (6) : 846. (June 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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