Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes

Autores
Manes, Jorgelina; Fiorentino, María Andrea; Kaiser, German Gustavo; Hozbor, Federico Andres; Alberio, Ricardo; Sánchez, Esteban O.; Paolicchi, Fernando
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The objective of this study was to characterize the vaginal bacterial flora and subsequent pregnancy rates after AI in sheep subjected to 4 different estrous synchronization regimes – the use of two intravaginal devices (silicone or polyurethane sponges), and two different treatment periods (7 or 14 days). Twenty-one multiparous Texel ewes were randomly allocated to 4 treatment groups during the breeding season. In the ewes from Group I (n = 6) and Group II (n = 5), a polyurethane sponge containing 60 mg MAP was inserted in the vagina for a period of 7 or 14 days, respectively. In the ewes of Group III (n = 5) and Group IV (n = 5), an intravaginal progesterone releasing insert (IVP4) containing 160 mg of progesterone in an inert silicone device, was inserted for 7 or 14 days, respectively. At device withdrawal, each ewe was treated with 200 IU eCG i.m. Standard bacteriological procedures were performed on vaginal mucus samples obtained before the introduction of the devices, at device withdrawal and on the day of AI in all groups. Estrus was recorded with the aid of vasectomized rams every 12 h, and AI was performed 52–54 h after device withdrawal, using fresh semen. The intervals between device withdrawal and estrus were: Group I: 56.4 ± 21.5 h; Group II: 42.0 ± 33.9 h; Group III: 51.6 ± 21.5 h; Group IV: 37.2 ± 10.7 h, while the pregnancy rates were: Group I: 83.3%; Group II: 60.0%; Group III: 60.0%; Group IV: 60.0%. The pregnancy rates and the interval between device withdrawal and the occurrence of estrus did not differ between treatments. The predominant bacterial flora population at device insertion was mostly gram positive (G+) (90%) bacteria. The strains most frequently found were Bacilllus sp., Staphylococcus sp. and Corynebacterium sp. Of the 19 isolates made at device removal, 79% were gram negative, with the Escherichia sp. being the most frequently isolated. At the time of AI and regardless of the device used, the 14-day treatment group presented an initial gram positive bacterial flora, while the 7-day groups presented gram negative flora (82%). It could be concluded that the use of intravaginal devices, regardless of their composition (silicone or polyurethane), may generate changes in the normal vaginal bacterial flora of the vaginal mucus. These changes did not reflect on the subsequent fertility. The use of intravaginal devices should however include the adoption of strict hygiene procedures, to minimize the growth of bacterial flora.
EEA Balcarce
Fil: Manes, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina.
Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; Argentina
Fil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fil: Sánchez, Esteban O. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina.
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; Argentina
Fuente
Small Ruminant Research 94 (1–3) : 201-204. (November 2010)
Materia
Oveja
Flora Microbiana
Ciclo Estral
Bacteria
Ewes
Microbial Flora
Oestrous Cycle
Microbiota Vaginal
Dispositivos Intravaginales
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4945

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network_name_str INTA Digital (INTA)
spelling Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewesManes, JorgelinaFiorentino, María AndreaKaiser, German GustavoHozbor, Federico AndresAlberio, RicardoSánchez, Esteban O.Paolicchi, FernandoOvejaFlora MicrobianaCiclo EstralBacteriaEwesMicrobial FloraOestrous CycleMicrobiota VaginalDispositivos IntravaginalesThe objective of this study was to characterize the vaginal bacterial flora and subsequent pregnancy rates after AI in sheep subjected to 4 different estrous synchronization regimes – the use of two intravaginal devices (silicone or polyurethane sponges), and two different treatment periods (7 or 14 days). Twenty-one multiparous Texel ewes were randomly allocated to 4 treatment groups during the breeding season. In the ewes from Group I (n = 6) and Group II (n = 5), a polyurethane sponge containing 60 mg MAP was inserted in the vagina for a period of 7 or 14 days, respectively. In the ewes of Group III (n = 5) and Group IV (n = 5), an intravaginal progesterone releasing insert (IVP4) containing 160 mg of progesterone in an inert silicone device, was inserted for 7 or 14 days, respectively. At device withdrawal, each ewe was treated with 200 IU eCG i.m. Standard bacteriological procedures were performed on vaginal mucus samples obtained before the introduction of the devices, at device withdrawal and on the day of AI in all groups. Estrus was recorded with the aid of vasectomized rams every 12 h, and AI was performed 52–54 h after device withdrawal, using fresh semen. The intervals between device withdrawal and estrus were: Group I: 56.4 ± 21.5 h; Group II: 42.0 ± 33.9 h; Group III: 51.6 ± 21.5 h; Group IV: 37.2 ± 10.7 h, while the pregnancy rates were: Group I: 83.3%; Group II: 60.0%; Group III: 60.0%; Group IV: 60.0%. The pregnancy rates and the interval between device withdrawal and the occurrence of estrus did not differ between treatments. The predominant bacterial flora population at device insertion was mostly gram positive (G+) (90%) bacteria. The strains most frequently found were Bacilllus sp., Staphylococcus sp. and Corynebacterium sp. Of the 19 isolates made at device removal, 79% were gram negative, with the Escherichia sp. being the most frequently isolated. At the time of AI and regardless of the device used, the 14-day treatment group presented an initial gram positive bacterial flora, while the 7-day groups presented gram negative flora (82%). It could be concluded that the use of intravaginal devices, regardless of their composition (silicone or polyurethane), may generate changes in the normal vaginal bacterial flora of the vaginal mucus. These changes did not reflect on the subsequent fertility. The use of intravaginal devices should however include the adoption of strict hygiene procedures, to minimize the growth of bacterial flora.EEA BalcarceFil: Manes, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina.Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; ArgentinaFil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; ArgentinaFil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; ArgentinaFil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; ArgentinaFil: Sánchez, Esteban O. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina.Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; ArgentinaElsevier2019-04-22T15:20:24Z2019-04-22T15:20:24Z2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0921448810002221http://hdl.handle.net/20.500.12123/49450921-4488https://doi.org/10.1016/j.smallrumres.2010.07.021Small Ruminant Research 94 (1–3) : 201-204. (November 2010)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:38Zoai:localhost:20.500.12123/4945instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:38.951INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
title Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
spellingShingle Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
Manes, Jorgelina
Oveja
Flora Microbiana
Ciclo Estral
Bacteria
Ewes
Microbial Flora
Oestrous Cycle
Microbiota Vaginal
Dispositivos Intravaginales
title_short Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
title_full Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
title_fullStr Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
title_full_unstemmed Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
title_sort Changes in the aerobic vaginal flora after treatment with different intravaginal devices in ewes
dc.creator.none.fl_str_mv Manes, Jorgelina
Fiorentino, María Andrea
Kaiser, German Gustavo
Hozbor, Federico Andres
Alberio, Ricardo
Sánchez, Esteban O.
Paolicchi, Fernando
author Manes, Jorgelina
author_facet Manes, Jorgelina
Fiorentino, María Andrea
Kaiser, German Gustavo
Hozbor, Federico Andres
Alberio, Ricardo
Sánchez, Esteban O.
Paolicchi, Fernando
author_role author
author2 Fiorentino, María Andrea
Kaiser, German Gustavo
Hozbor, Federico Andres
Alberio, Ricardo
Sánchez, Esteban O.
Paolicchi, Fernando
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Oveja
Flora Microbiana
Ciclo Estral
Bacteria
Ewes
Microbial Flora
Oestrous Cycle
Microbiota Vaginal
Dispositivos Intravaginales
topic Oveja
Flora Microbiana
Ciclo Estral
Bacteria
Ewes
Microbial Flora
Oestrous Cycle
Microbiota Vaginal
Dispositivos Intravaginales
dc.description.none.fl_txt_mv The objective of this study was to characterize the vaginal bacterial flora and subsequent pregnancy rates after AI in sheep subjected to 4 different estrous synchronization regimes – the use of two intravaginal devices (silicone or polyurethane sponges), and two different treatment periods (7 or 14 days). Twenty-one multiparous Texel ewes were randomly allocated to 4 treatment groups during the breeding season. In the ewes from Group I (n = 6) and Group II (n = 5), a polyurethane sponge containing 60 mg MAP was inserted in the vagina for a period of 7 or 14 days, respectively. In the ewes of Group III (n = 5) and Group IV (n = 5), an intravaginal progesterone releasing insert (IVP4) containing 160 mg of progesterone in an inert silicone device, was inserted for 7 or 14 days, respectively. At device withdrawal, each ewe was treated with 200 IU eCG i.m. Standard bacteriological procedures were performed on vaginal mucus samples obtained before the introduction of the devices, at device withdrawal and on the day of AI in all groups. Estrus was recorded with the aid of vasectomized rams every 12 h, and AI was performed 52–54 h after device withdrawal, using fresh semen. The intervals between device withdrawal and estrus were: Group I: 56.4 ± 21.5 h; Group II: 42.0 ± 33.9 h; Group III: 51.6 ± 21.5 h; Group IV: 37.2 ± 10.7 h, while the pregnancy rates were: Group I: 83.3%; Group II: 60.0%; Group III: 60.0%; Group IV: 60.0%. The pregnancy rates and the interval between device withdrawal and the occurrence of estrus did not differ between treatments. The predominant bacterial flora population at device insertion was mostly gram positive (G+) (90%) bacteria. The strains most frequently found were Bacilllus sp., Staphylococcus sp. and Corynebacterium sp. Of the 19 isolates made at device removal, 79% were gram negative, with the Escherichia sp. being the most frequently isolated. At the time of AI and regardless of the device used, the 14-day treatment group presented an initial gram positive bacterial flora, while the 7-day groups presented gram negative flora (82%). It could be concluded that the use of intravaginal devices, regardless of their composition (silicone or polyurethane), may generate changes in the normal vaginal bacterial flora of the vaginal mucus. These changes did not reflect on the subsequent fertility. The use of intravaginal devices should however include the adoption of strict hygiene procedures, to minimize the growth of bacterial flora.
EEA Balcarce
Fil: Manes, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina.
Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; Argentina
Fil: Kaiser, German Gustavo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fil: Hozbor, Federico Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fil: Alberio, Ricardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina
Fil: Sánchez, Esteban O. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina.
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; Argentina
description The objective of this study was to characterize the vaginal bacterial flora and subsequent pregnancy rates after AI in sheep subjected to 4 different estrous synchronization regimes – the use of two intravaginal devices (silicone or polyurethane sponges), and two different treatment periods (7 or 14 days). Twenty-one multiparous Texel ewes were randomly allocated to 4 treatment groups during the breeding season. In the ewes from Group I (n = 6) and Group II (n = 5), a polyurethane sponge containing 60 mg MAP was inserted in the vagina for a period of 7 or 14 days, respectively. In the ewes of Group III (n = 5) and Group IV (n = 5), an intravaginal progesterone releasing insert (IVP4) containing 160 mg of progesterone in an inert silicone device, was inserted for 7 or 14 days, respectively. At device withdrawal, each ewe was treated with 200 IU eCG i.m. Standard bacteriological procedures were performed on vaginal mucus samples obtained before the introduction of the devices, at device withdrawal and on the day of AI in all groups. Estrus was recorded with the aid of vasectomized rams every 12 h, and AI was performed 52–54 h after device withdrawal, using fresh semen. The intervals between device withdrawal and estrus were: Group I: 56.4 ± 21.5 h; Group II: 42.0 ± 33.9 h; Group III: 51.6 ± 21.5 h; Group IV: 37.2 ± 10.7 h, while the pregnancy rates were: Group I: 83.3%; Group II: 60.0%; Group III: 60.0%; Group IV: 60.0%. The pregnancy rates and the interval between device withdrawal and the occurrence of estrus did not differ between treatments. The predominant bacterial flora population at device insertion was mostly gram positive (G+) (90%) bacteria. The strains most frequently found were Bacilllus sp., Staphylococcus sp. and Corynebacterium sp. Of the 19 isolates made at device removal, 79% were gram negative, with the Escherichia sp. being the most frequently isolated. At the time of AI and regardless of the device used, the 14-day treatment group presented an initial gram positive bacterial flora, while the 7-day groups presented gram negative flora (82%). It could be concluded that the use of intravaginal devices, regardless of their composition (silicone or polyurethane), may generate changes in the normal vaginal bacterial flora of the vaginal mucus. These changes did not reflect on the subsequent fertility. The use of intravaginal devices should however include the adoption of strict hygiene procedures, to minimize the growth of bacterial flora.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
2019-04-22T15:20:24Z
2019-04-22T15:20:24Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0921448810002221
http://hdl.handle.net/20.500.12123/4945
0921-4488
https://doi.org/10.1016/j.smallrumres.2010.07.021
url https://www.sciencedirect.com/science/article/pii/S0921448810002221
http://hdl.handle.net/20.500.12123/4945
https://doi.org/10.1016/j.smallrumres.2010.07.021
identifier_str_mv 0921-4488
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Small Ruminant Research 94 (1–3) : 201-204. (November 2010)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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