Vaginal bacterial diversity from healthy gilts and pregnant sows subjected to natural mating or artificial insemination

Autores
Torres Luque, Andrea; Fontana, Cecilia Alejandra; Pasteris, Sergio E.; Bassi, Daniela; Cocconcelli, Pier Sandro; Otero, María Claudia
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
The profitability of commercial pig farms largely depends on the reproductive performance of gilts and sows. The aim of this study was to identify differences in the composition and diversity of vaginal microbiota between gilts (G) and pregnant (P) sows, both artificially inseminated (AI) and natural mated (NM). Samples were collected by scraping the vaginal mucosa of G (n = 10) and P (NM, n = 10 and AI, n = 7) sows. Samples were analysed by culture-dependent techniques and 16S-rRNA gene High-Throughput-Sequencing. The profiles of the cultured microbiota showed two distinctive clusters, one of them grouped four samples of P sows from the AI group. The vaginal microbiota from P had lower richness than G sows (Mann-Whitney/Kruskal-Wallis test, p < 0.01), but all vaginal samples had a similar diversity. The PERMANOVA analyses revealed significant differences (p < 0.01) between the microbial communities' structures from G and P sows. The bacteria phyla with the highest relative abundances was Proteobacteria (33.1%), followed by Firmicutes (32%), Cyanobacteria (13.3%) and Actinobacteria (13.2%). The relative abundance for phyla, families and genera was estimated and Proteobacteria was significantly higher (p = 0.038) in P than in G sows; Firmicutes was significantly lower in AI than G and NM sows. A “core microbiota” included Lactobacillus, Bacillus, Enterococcus, Acinetobacter, and Pseudomonas. The results presented highlight the differences in the bacterial composition between G and P sows, as well as the changes in the microbial populations associated with the breeding method.
EEA Famaillá
Fil: Torres Luque, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Torres Luque, Andrea. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Fontana, Cecilia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Pasteris, Sergio E. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pasteris, Sergio E. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bassi, Daniela. Università Cattolica del Sacro Cuore. Dipartimento di Scienze e Tecnologie Alimentari per una filiera agro-alimentare Sostenibile; Italia
Fil: Cocconcelli, Pier Sandro. Universita Cattolica del Sacro Cuore. Dipartimento di Scienze e Tecnologie Alimentari per una filiera agro-alimentare Sostenibile ; Italia
Fil: Otero, María Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Otero, María Claudia. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fuente
Research in Veterinary Science (Available online 28 July 2021)
Materia
Cerdo
Genética
Gestación
Swine
Genetics
Pregnancy
Sows
Gilts
Microbial Flora
Cerdas
Flora Microbiana
Microbiota
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9964
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9964
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Vaginal bacterial diversity from healthy gilts and pregnant sows subjected to natural mating or artificial inseminationTorres Luque, AndreaFontana, Cecilia AlejandraPasteris, Sergio E.Bassi, DanielaCocconcelli, Pier SandroOtero, María ClaudiaCerdoGenéticaGestaciónSwineGeneticsPregnancySowsGiltsMicrobial FloraCerdasFlora MicrobianaMicrobiotaThe profitability of commercial pig farms largely depends on the reproductive performance of gilts and sows. The aim of this study was to identify differences in the composition and diversity of vaginal microbiota between gilts (G) and pregnant (P) sows, both artificially inseminated (AI) and natural mated (NM). Samples were collected by scraping the vaginal mucosa of G (n = 10) and P (NM, n = 10 and AI, n = 7) sows. Samples were analysed by culture-dependent techniques and 16S-rRNA gene High-Throughput-Sequencing. The profiles of the cultured microbiota showed two distinctive clusters, one of them grouped four samples of P sows from the AI group. The vaginal microbiota from P had lower richness than G sows (Mann-Whitney/Kruskal-Wallis test, p < 0.01), but all vaginal samples had a similar diversity. The PERMANOVA analyses revealed significant differences (p < 0.01) between the microbial communities' structures from G and P sows. The bacteria phyla with the highest relative abundances was Proteobacteria (33.1%), followed by Firmicutes (32%), Cyanobacteria (13.3%) and Actinobacteria (13.2%). The relative abundance for phyla, families and genera was estimated and Proteobacteria was significantly higher (p = 0.038) in P than in G sows; Firmicutes was significantly lower in AI than G and NM sows. A “core microbiota” included Lactobacillus, Bacillus, Enterococcus, Acinetobacter, and Pseudomonas. The results presented highlight the differences in the bacterial composition between G and P sows, as well as the changes in the microbial populations associated with the breeding method.EEA FamailláFil: Torres Luque, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Torres Luque, Andrea. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Fontana, Cecilia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; ArgentinaFil: Pasteris, Sergio E. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Pasteris, Sergio E. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Bassi, Daniela. Università Cattolica del Sacro Cuore. Dipartimento di Scienze e Tecnologie Alimentari per una filiera agro-alimentare Sostenibile; ItaliaFil: Cocconcelli, Pier Sandro. Universita Cattolica del Sacro Cuore. Dipartimento di Scienze e Tecnologie Alimentari per una filiera agro-alimentare Sostenibile ; ItaliaFil: Otero, María Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaFil: Otero, María Claudia. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; ArgentinaElsevierinfo:eu-repo/date/embargoEnd/2022-08-052021-08-05T14:14:11Z2021-08-05T14:14:11Z2021-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9964https://www.sciencedirect.com/science/article/abs/pii/S00345288210023810034-5288https://doi.org/10.1016/j.rvsc.2021.07.023Research in Veterinary Science (Available online 28 July 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:18Zoai:localhost:20.500.12123/9964instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:18.52INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Vaginal bacterial diversity from healthy gilts and pregnant sows subjected to natural mating or artificial insemination
title Vaginal bacterial diversity from healthy gilts and pregnant sows subjected to natural mating or artificial insemination
spellingShingle Vaginal bacterial diversity from healthy gilts and pregnant sows subjected to natural mating or artificial insemination
Torres Luque, Andrea
Cerdo
Genética
Gestación
Swine
Genetics
Pregnancy
Sows
Gilts
Microbial Flora
Cerdas
Flora Microbiana
Microbiota
title_short Vaginal bacterial diversity from healthy gilts and pregnant sows subjected to natural mating or artificial insemination
title_full Vaginal bacterial diversity from healthy gilts and pregnant sows subjected to natural mating or artificial insemination
title_fullStr Vaginal bacterial diversity from healthy gilts and pregnant sows subjected to natural mating or artificial insemination
title_full_unstemmed Vaginal bacterial diversity from healthy gilts and pregnant sows subjected to natural mating or artificial insemination
title_sort Vaginal bacterial diversity from healthy gilts and pregnant sows subjected to natural mating or artificial insemination
dc.creator.none.fl_str_mv Torres Luque, Andrea
Fontana, Cecilia Alejandra
Pasteris, Sergio E.
Bassi, Daniela
Cocconcelli, Pier Sandro
Otero, María Claudia
author Torres Luque, Andrea
author_facet Torres Luque, Andrea
Fontana, Cecilia Alejandra
Pasteris, Sergio E.
Bassi, Daniela
Cocconcelli, Pier Sandro
Otero, María Claudia
author_role author
author2 Fontana, Cecilia Alejandra
Pasteris, Sergio E.
Bassi, Daniela
Cocconcelli, Pier Sandro
Otero, María Claudia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Cerdo
Genética
Gestación
Swine
Genetics
Pregnancy
Sows
Gilts
Microbial Flora
Cerdas
Flora Microbiana
Microbiota
topic Cerdo
Genética
Gestación
Swine
Genetics
Pregnancy
Sows
Gilts
Microbial Flora
Cerdas
Flora Microbiana
Microbiota
dc.description.none.fl_txt_mv The profitability of commercial pig farms largely depends on the reproductive performance of gilts and sows. The aim of this study was to identify differences in the composition and diversity of vaginal microbiota between gilts (G) and pregnant (P) sows, both artificially inseminated (AI) and natural mated (NM). Samples were collected by scraping the vaginal mucosa of G (n = 10) and P (NM, n = 10 and AI, n = 7) sows. Samples were analysed by culture-dependent techniques and 16S-rRNA gene High-Throughput-Sequencing. The profiles of the cultured microbiota showed two distinctive clusters, one of them grouped four samples of P sows from the AI group. The vaginal microbiota from P had lower richness than G sows (Mann-Whitney/Kruskal-Wallis test, p < 0.01), but all vaginal samples had a similar diversity. The PERMANOVA analyses revealed significant differences (p < 0.01) between the microbial communities' structures from G and P sows. The bacteria phyla with the highest relative abundances was Proteobacteria (33.1%), followed by Firmicutes (32%), Cyanobacteria (13.3%) and Actinobacteria (13.2%). The relative abundance for phyla, families and genera was estimated and Proteobacteria was significantly higher (p = 0.038) in P than in G sows; Firmicutes was significantly lower in AI than G and NM sows. A “core microbiota” included Lactobacillus, Bacillus, Enterococcus, Acinetobacter, and Pseudomonas. The results presented highlight the differences in the bacterial composition between G and P sows, as well as the changes in the microbial populations associated with the breeding method.
EEA Famaillá
Fil: Torres Luque, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Torres Luque, Andrea. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Fontana, Cecilia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Famaillá; Argentina
Fil: Pasteris, Sergio E. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Pasteris, Sergio E. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Bassi, Daniela. Università Cattolica del Sacro Cuore. Dipartimento di Scienze e Tecnologie Alimentari per una filiera agro-alimentare Sostenibile; Italia
Fil: Cocconcelli, Pier Sandro. Universita Cattolica del Sacro Cuore. Dipartimento di Scienze e Tecnologie Alimentari per una filiera agro-alimentare Sostenibile ; Italia
Fil: Otero, María Claudia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
Fil: Otero, María Claudia. Universidad Nacional de Tucumán. Instituto Superior de Investigaciones Biológicas; Argentina
description The profitability of commercial pig farms largely depends on the reproductive performance of gilts and sows. The aim of this study was to identify differences in the composition and diversity of vaginal microbiota between gilts (G) and pregnant (P) sows, both artificially inseminated (AI) and natural mated (NM). Samples were collected by scraping the vaginal mucosa of G (n = 10) and P (NM, n = 10 and AI, n = 7) sows. Samples were analysed by culture-dependent techniques and 16S-rRNA gene High-Throughput-Sequencing. The profiles of the cultured microbiota showed two distinctive clusters, one of them grouped four samples of P sows from the AI group. The vaginal microbiota from P had lower richness than G sows (Mann-Whitney/Kruskal-Wallis test, p < 0.01), but all vaginal samples had a similar diversity. The PERMANOVA analyses revealed significant differences (p < 0.01) between the microbial communities' structures from G and P sows. The bacteria phyla with the highest relative abundances was Proteobacteria (33.1%), followed by Firmicutes (32%), Cyanobacteria (13.3%) and Actinobacteria (13.2%). The relative abundance for phyla, families and genera was estimated and Proteobacteria was significantly higher (p = 0.038) in P than in G sows; Firmicutes was significantly lower in AI than G and NM sows. A “core microbiota” included Lactobacillus, Bacillus, Enterococcus, Acinetobacter, and Pseudomonas. The results presented highlight the differences in the bacterial composition between G and P sows, as well as the changes in the microbial populations associated with the breeding method.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-05T14:14:11Z
2021-08-05T14:14:11Z
2021-07
info:eu-repo/date/embargoEnd/2022-08-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9964
https://www.sciencedirect.com/science/article/abs/pii/S0034528821002381
0034-5288
https://doi.org/10.1016/j.rvsc.2021.07.023
url http://hdl.handle.net/20.500.12123/9964
https://www.sciencedirect.com/science/article/abs/pii/S0034528821002381
https://doi.org/10.1016/j.rvsc.2021.07.023
identifier_str_mv 0034-5288
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Research in Veterinary Science (Available online 28 July 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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