Diversity structure of the microbial communities in the guts of four neotropical termite species

Autores
Vikram, Surendra; Arneodo Larochette, Joel Demian; Calcagno, Javier; Ortiz, Maximiliano; Mon, Maria Laura; Etcheverry, Clara; Cowan, Donald; Talia, Paola Mónica
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (soil organic matter/grass) and Cornitermes cumulans (grass) by deep sequencing of amplified 16S rRNA and ITS genes. In addition, we have performed a taxonomic and gut community structure comparison incorporating into the analysis the previously reported microbiomes of additional termite species with varied diets. The bacterial phylum Spirochaetes was dominant in the guts of M. strunckii, N. corniger and C. cumulans, whereas Firmicutes predominated in the T. riograndensis gut microbiome. A single bacterial genus, Treponema (Spirochaetes), was dominant in all termite species, except for T. riograndensis. Both in our own sequenced samples and in the broader comparison, prokaryotic α-diversity was higher in the soil/grass feeders than in the wood feeders. Meanwhile, the β-diversity of prokaryotes and fungi was highly dissimilar among strict wood-feeders, whereas that of soil- and grass-feeders grouped more closely. Ascomycota and Basidiomycota were the only fungal phyla that could be identified in all gut samples, because of the lack of reference sequences in public databases. In summary, higher microbial diversity was recorded in termites with more versatile feeding sources, providing further evidence that diet, along with other factors (e.g., host taxonomy), influences the microbial community assembly in the termite gut.
Instituto de Biotecnología
Fil: Vikram, Surendra. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica
Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Arneodo Larochette, Joel Demian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calcagno, Javier. Universidad Maimonides. Centro de Ciencias Naturales, Ambientales y Antropológicas; Argentina
Fil: Ortiz, Maximiliano. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica
Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Etcheverry, Clara. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Biología de los Invertebrados; Argentina
Fil: Cowan, Donald. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica
Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
PeerJ. 9 : e10959 (Abril 2021)
Materia
Isoptera
Flora Microbiana
Termitidae
Microbial Flora
Prokaryotae
Microbiota
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10238
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oai_identifier_str oai:localhost:20.500.12123/10238
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Diversity structure of the microbial communities in the guts of four neotropical termite speciesVikram, SurendraArneodo Larochette, Joel DemianCalcagno, JavierOrtiz, MaximilianoMon, Maria LauraEtcheverry, ClaraCowan, DonaldTalia, Paola MónicaIsopteraFlora MicrobianaTermitidaeMicrobial FloraProkaryotaeMicrobiotaThe termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (soil organic matter/grass) and Cornitermes cumulans (grass) by deep sequencing of amplified 16S rRNA and ITS genes. In addition, we have performed a taxonomic and gut community structure comparison incorporating into the analysis the previously reported microbiomes of additional termite species with varied diets. The bacterial phylum Spirochaetes was dominant in the guts of M. strunckii, N. corniger and C. cumulans, whereas Firmicutes predominated in the T. riograndensis gut microbiome. A single bacterial genus, Treponema (Spirochaetes), was dominant in all termite species, except for T. riograndensis. Both in our own sequenced samples and in the broader comparison, prokaryotic α-diversity was higher in the soil/grass feeders than in the wood feeders. Meanwhile, the β-diversity of prokaryotes and fungi was highly dissimilar among strict wood-feeders, whereas that of soil- and grass-feeders grouped more closely. Ascomycota and Basidiomycota were the only fungal phyla that could be identified in all gut samples, because of the lack of reference sequences in public databases. In summary, higher microbial diversity was recorded in termites with more versatile feeding sources, providing further evidence that diet, along with other factors (e.g., host taxonomy), influences the microbial community assembly in the termite gut.Instituto de BiotecnologíaFil: Vikram, Surendra. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; SudáfricaFil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Arneodo Larochette, Joel Demian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Calcagno, Javier. Universidad Maimonides. Centro de Ciencias Naturales, Ambientales y Antropológicas; ArgentinaFil: Ortiz, Maximiliano. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; SudáfricaFil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Etcheverry, Clara. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Biología de los Invertebrados; ArgentinaFil: Cowan, Donald. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; SudáfricaFil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaPeer J Inc.2021-09-13T14:14:46Z2021-09-13T14:14:46Z2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10238https://peerj.com/articles/10959/2167-8359https://doi.org/10.7717/peerj.10959PeerJ. 9 : e10959 (Abril 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNAIyAV-1130034/AR./Desarrollo de procesos para la transformación de biomasa en bioenergía.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:05Zoai:localhost:20.500.12123/10238instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:06.151INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Diversity structure of the microbial communities in the guts of four neotropical termite species
title Diversity structure of the microbial communities in the guts of four neotropical termite species
spellingShingle Diversity structure of the microbial communities in the guts of four neotropical termite species
Vikram, Surendra
Isoptera
Flora Microbiana
Termitidae
Microbial Flora
Prokaryotae
Microbiota
title_short Diversity structure of the microbial communities in the guts of four neotropical termite species
title_full Diversity structure of the microbial communities in the guts of four neotropical termite species
title_fullStr Diversity structure of the microbial communities in the guts of four neotropical termite species
title_full_unstemmed Diversity structure of the microbial communities in the guts of four neotropical termite species
title_sort Diversity structure of the microbial communities in the guts of four neotropical termite species
dc.creator.none.fl_str_mv Vikram, Surendra
Arneodo Larochette, Joel Demian
Calcagno, Javier
Ortiz, Maximiliano
Mon, Maria Laura
Etcheverry, Clara
Cowan, Donald
Talia, Paola Mónica
author Vikram, Surendra
author_facet Vikram, Surendra
Arneodo Larochette, Joel Demian
Calcagno, Javier
Ortiz, Maximiliano
Mon, Maria Laura
Etcheverry, Clara
Cowan, Donald
Talia, Paola Mónica
author_role author
author2 Arneodo Larochette, Joel Demian
Calcagno, Javier
Ortiz, Maximiliano
Mon, Maria Laura
Etcheverry, Clara
Cowan, Donald
Talia, Paola Mónica
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Isoptera
Flora Microbiana
Termitidae
Microbial Flora
Prokaryotae
Microbiota
topic Isoptera
Flora Microbiana
Termitidae
Microbial Flora
Prokaryotae
Microbiota
dc.description.none.fl_txt_mv The termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (soil organic matter/grass) and Cornitermes cumulans (grass) by deep sequencing of amplified 16S rRNA and ITS genes. In addition, we have performed a taxonomic and gut community structure comparison incorporating into the analysis the previously reported microbiomes of additional termite species with varied diets. The bacterial phylum Spirochaetes was dominant in the guts of M. strunckii, N. corniger and C. cumulans, whereas Firmicutes predominated in the T. riograndensis gut microbiome. A single bacterial genus, Treponema (Spirochaetes), was dominant in all termite species, except for T. riograndensis. Both in our own sequenced samples and in the broader comparison, prokaryotic α-diversity was higher in the soil/grass feeders than in the wood feeders. Meanwhile, the β-diversity of prokaryotes and fungi was highly dissimilar among strict wood-feeders, whereas that of soil- and grass-feeders grouped more closely. Ascomycota and Basidiomycota were the only fungal phyla that could be identified in all gut samples, because of the lack of reference sequences in public databases. In summary, higher microbial diversity was recorded in termites with more versatile feeding sources, providing further evidence that diet, along with other factors (e.g., host taxonomy), influences the microbial community assembly in the termite gut.
Instituto de Biotecnología
Fil: Vikram, Surendra. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica
Fil: Arneodo Larochette, Joel Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Arneodo Larochette, Joel Demian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Calcagno, Javier. Universidad Maimonides. Centro de Ciencias Naturales, Ambientales y Antropológicas; Argentina
Fil: Ortiz, Maximiliano. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica
Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Etcheverry, Clara. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura. Biología de los Invertebrados; Argentina
Fil: Cowan, Donald. University of Pretoria. Centre for Microbial Ecology and Genomics. Department Biochemistry. Genetics and Microbiology; Sudáfrica
Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The termite gut microbiome is dominated by lignocellulose degrading microorganisms. This study describes the intestinal microbiota of four Argentinian higher termite species with different feeding habits: Microcerotermes strunckii (hardwood), Nasutitermes corniger (softwood), Termes riograndensis (soil organic matter/grass) and Cornitermes cumulans (grass) by deep sequencing of amplified 16S rRNA and ITS genes. In addition, we have performed a taxonomic and gut community structure comparison incorporating into the analysis the previously reported microbiomes of additional termite species with varied diets. The bacterial phylum Spirochaetes was dominant in the guts of M. strunckii, N. corniger and C. cumulans, whereas Firmicutes predominated in the T. riograndensis gut microbiome. A single bacterial genus, Treponema (Spirochaetes), was dominant in all termite species, except for T. riograndensis. Both in our own sequenced samples and in the broader comparison, prokaryotic α-diversity was higher in the soil/grass feeders than in the wood feeders. Meanwhile, the β-diversity of prokaryotes and fungi was highly dissimilar among strict wood-feeders, whereas that of soil- and grass-feeders grouped more closely. Ascomycota and Basidiomycota were the only fungal phyla that could be identified in all gut samples, because of the lack of reference sequences in public databases. In summary, higher microbial diversity was recorded in termites with more versatile feeding sources, providing further evidence that diet, along with other factors (e.g., host taxonomy), influences the microbial community assembly in the termite gut.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-13T14:14:46Z
2021-09-13T14:14:46Z
2021-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10238
https://peerj.com/articles/10959/
2167-8359
https://doi.org/10.7717/peerj.10959
url http://hdl.handle.net/20.500.12123/10238
https://peerj.com/articles/10959/
https://doi.org/10.7717/peerj.10959
identifier_str_mv 2167-8359
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNAIyAV-1130034/AR./Desarrollo de procesos para la transformación de biomasa en bioenergía.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Peer J Inc.
publisher.none.fl_str_mv Peer J Inc.
dc.source.none.fl_str_mv PeerJ. 9 : e10959 (Abril 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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