Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle

Autores
Manes, Jorgelina; Fiorentino, María Andrea; San Martino, Silvina; Ungerfeld, Rodolfo
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aims of this study were to determine if the ewe’ vaginal bacterial load differs according to the stage of the oestrous cycle; and thus, if the stage of the oestrous cycle when intravaginal sponges (IS) used for oestrous synchronisation are inserted influences the vaginal bacterial biota present at sponge withdrawal. During the breeding season, 45 Texel ewes were detected in oestrus and randomly allocated to one of three treatments of 15 ewes each according to the stage of the oestrous cycle when the IS were inserted. Intravaginal sponges were inserted: the day of oestrus (GOes), or 2 (GMet) or 8 (GDie) days after oestrus. Samples for bacterial analysis were collected the day of IS insertion (Day 0), at sponge withdrawal (Day 12), 24 h (Day 13) and 48 h (Day 14) after sponge withdrawal. Vaginal bacteria amount increased at sponge withdrawal in all treatment (p<0.0001). The number of different types of colonies was greater in GMet group than in GDie at sponge insertion (p<0.05), without difference with GOes. At sponge insertion, the presence of Staphylococcus spp. was greater in GMet than in GOe and GDi (p<0.05); the presence of Trueperella pyogenes and Haemophilus somni was greater after sponge withdrawal than before sponge insertion (p<0.001), but without difference between days 12, 13 and 14. Overall, we concluded that in ewes the normal vaginal bacterial load differs according to the stage of the oestrous cycle. The continuous presence of the IS homogenised the vaginal bacterial microbiota at withdrawal, with only slight and transient differences observed on day 13.
Fil: Manes, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; Uruguay
Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; Argentina
Fil: San Martino, Silvina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Departamento de Introducción a las Ciencias Agrarias. Estadística y Diseño Experimental; Argentina
Fil: Ungerfeld, Rodolfo. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; Uruguay
Fuente
Livestock science 208 : 55–59. (2018)
Materia
Flora Microbiana
Vagina
Sincronización del Celo
Ciclo Estral
Vaginitis
Oveja
Ewes
Oestrous Cycle
Oestrus Synchronization
Microbial Flora
Microbiota Vaginal
Esponjas Intravaginales
Intravaginal Sponges
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2759
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2759
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycleManes, JorgelinaFiorentino, María AndreaSan Martino, SilvinaUngerfeld, RodolfoFlora MicrobianaVaginaSincronización del CeloCiclo EstralVaginitisOvejaEwesOestrous CycleOestrus SynchronizationMicrobial FloraMicrobiota VaginalEsponjas IntravaginalesIntravaginal SpongesThe aims of this study were to determine if the ewe’ vaginal bacterial load differs according to the stage of the oestrous cycle; and thus, if the stage of the oestrous cycle when intravaginal sponges (IS) used for oestrous synchronisation are inserted influences the vaginal bacterial biota present at sponge withdrawal. During the breeding season, 45 Texel ewes were detected in oestrus and randomly allocated to one of three treatments of 15 ewes each according to the stage of the oestrous cycle when the IS were inserted. Intravaginal sponges were inserted: the day of oestrus (GOes), or 2 (GMet) or 8 (GDie) days after oestrus. Samples for bacterial analysis were collected the day of IS insertion (Day 0), at sponge withdrawal (Day 12), 24 h (Day 13) and 48 h (Day 14) after sponge withdrawal. Vaginal bacteria amount increased at sponge withdrawal in all treatment (p<0.0001). The number of different types of colonies was greater in GMet group than in GDie at sponge insertion (p<0.05), without difference with GOes. At sponge insertion, the presence of Staphylococcus spp. was greater in GMet than in GOe and GDi (p<0.05); the presence of Trueperella pyogenes and Haemophilus somni was greater after sponge withdrawal than before sponge insertion (p<0.001), but without difference between days 12, 13 and 14. Overall, we concluded that in ewes the normal vaginal bacterial load differs according to the stage of the oestrous cycle. The continuous presence of the IS homogenised the vaginal bacterial microbiota at withdrawal, with only slight and transient differences observed on day 13.Fil: Manes, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; UruguayFil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; ArgentinaFil: San Martino, Silvina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Departamento de Introducción a las Ciencias Agrarias. Estadística y Diseño Experimental; ArgentinaFil: Ungerfeld, Rodolfo. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; Uruguay2018-07-10T18:26:28Z2018-07-10T18:26:28Z2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/27591871-1413https://doi.org/10.1016/j.livsci.2017.11.023Livestock science 208 : 55–59. (2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:21Zoai:localhost:20.500.12123/2759instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:22.167INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle
title Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle
spellingShingle Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle
Manes, Jorgelina
Flora Microbiana
Vagina
Sincronización del Celo
Ciclo Estral
Vaginitis
Oveja
Ewes
Oestrous Cycle
Oestrus Synchronization
Microbial Flora
Microbiota Vaginal
Esponjas Intravaginales
Intravaginal Sponges
title_short Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle
title_full Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle
title_fullStr Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle
title_full_unstemmed Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle
title_sort Changes in the vaginal microbiota in ewes after insertion of intravaginal sponges at different stages of the oestrous cycle
dc.creator.none.fl_str_mv Manes, Jorgelina
Fiorentino, María Andrea
San Martino, Silvina
Ungerfeld, Rodolfo
author Manes, Jorgelina
author_facet Manes, Jorgelina
Fiorentino, María Andrea
San Martino, Silvina
Ungerfeld, Rodolfo
author_role author
author2 Fiorentino, María Andrea
San Martino, Silvina
Ungerfeld, Rodolfo
author2_role author
author
author
dc.subject.none.fl_str_mv Flora Microbiana
Vagina
Sincronización del Celo
Ciclo Estral
Vaginitis
Oveja
Ewes
Oestrous Cycle
Oestrus Synchronization
Microbial Flora
Microbiota Vaginal
Esponjas Intravaginales
Intravaginal Sponges
topic Flora Microbiana
Vagina
Sincronización del Celo
Ciclo Estral
Vaginitis
Oveja
Ewes
Oestrous Cycle
Oestrus Synchronization
Microbial Flora
Microbiota Vaginal
Esponjas Intravaginales
Intravaginal Sponges
dc.description.none.fl_txt_mv The aims of this study were to determine if the ewe’ vaginal bacterial load differs according to the stage of the oestrous cycle; and thus, if the stage of the oestrous cycle when intravaginal sponges (IS) used for oestrous synchronisation are inserted influences the vaginal bacterial biota present at sponge withdrawal. During the breeding season, 45 Texel ewes were detected in oestrus and randomly allocated to one of three treatments of 15 ewes each according to the stage of the oestrous cycle when the IS were inserted. Intravaginal sponges were inserted: the day of oestrus (GOes), or 2 (GMet) or 8 (GDie) days after oestrus. Samples for bacterial analysis were collected the day of IS insertion (Day 0), at sponge withdrawal (Day 12), 24 h (Day 13) and 48 h (Day 14) after sponge withdrawal. Vaginal bacteria amount increased at sponge withdrawal in all treatment (p<0.0001). The number of different types of colonies was greater in GMet group than in GDie at sponge insertion (p<0.05), without difference with GOes. At sponge insertion, the presence of Staphylococcus spp. was greater in GMet than in GOe and GDi (p<0.05); the presence of Trueperella pyogenes and Haemophilus somni was greater after sponge withdrawal than before sponge insertion (p<0.001), but without difference between days 12, 13 and 14. Overall, we concluded that in ewes the normal vaginal bacterial load differs according to the stage of the oestrous cycle. The continuous presence of the IS homogenised the vaginal bacterial microbiota at withdrawal, with only slight and transient differences observed on day 13.
Fil: Manes, Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Biotecnología de la Reproducción; Argentina. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; Uruguay
Fil: Fiorentino, María Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Departamento de Producción Animal. Laboratorio de Bacteriología; Argentina
Fil: San Martino, Silvina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias. Departamento de Introducción a las Ciencias Agrarias. Estadística y Diseño Experimental; Argentina
Fil: Ungerfeld, Rodolfo. Universidad de la República. Facultad de Veterinaria. Departamento de Fisiología; Uruguay
description The aims of this study were to determine if the ewe’ vaginal bacterial load differs according to the stage of the oestrous cycle; and thus, if the stage of the oestrous cycle when intravaginal sponges (IS) used for oestrous synchronisation are inserted influences the vaginal bacterial biota present at sponge withdrawal. During the breeding season, 45 Texel ewes were detected in oestrus and randomly allocated to one of three treatments of 15 ewes each according to the stage of the oestrous cycle when the IS were inserted. Intravaginal sponges were inserted: the day of oestrus (GOes), or 2 (GMet) or 8 (GDie) days after oestrus. Samples for bacterial analysis were collected the day of IS insertion (Day 0), at sponge withdrawal (Day 12), 24 h (Day 13) and 48 h (Day 14) after sponge withdrawal. Vaginal bacteria amount increased at sponge withdrawal in all treatment (p<0.0001). The number of different types of colonies was greater in GMet group than in GDie at sponge insertion (p<0.05), without difference with GOes. At sponge insertion, the presence of Staphylococcus spp. was greater in GMet than in GOe and GDi (p<0.05); the presence of Trueperella pyogenes and Haemophilus somni was greater after sponge withdrawal than before sponge insertion (p<0.001), but without difference between days 12, 13 and 14. Overall, we concluded that in ewes the normal vaginal bacterial load differs according to the stage of the oestrous cycle. The continuous presence of the IS homogenised the vaginal bacterial microbiota at withdrawal, with only slight and transient differences observed on day 13.
publishDate 2018
dc.date.none.fl_str_mv 2018-07-10T18:26:28Z
2018-07-10T18:26:28Z
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2759
1871-1413
https://doi.org/10.1016/j.livsci.2017.11.023
url http://hdl.handle.net/20.500.12123/2759
https://doi.org/10.1016/j.livsci.2017.11.023
identifier_str_mv 1871-1413
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Livestock science 208 : 55–59. (2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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