Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress

Autores
Palacios, María Belén; Rizzo, Alex Joel; Heredia, Tatiana Belén; Roqueiro, Gonzalo; Maldonado, Sara; Murgida, Daniel Horacio; Burrieza, Hernán Pablo
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Quinoa is a facultative halophyte with excellent tolerance to salinity. In this study, the epidermal bladder cell complex (EBCc) of quinoa leaves was studied to determine their cellular characteristics and involvement in salt tolerance. We used light microscopy, confocal RAMAN microscopy, confocal fuorescence microscopy, transmission electron microscopy, and environmental scanning electron microscopy complemented by energy dispersive X-ray analysis. Ionic content was quantifed with fame atomic absorption spectroscopy and with fame emission photometry. Results show that: (i) the number of EBCcs remains constant but their density and area vary with leaf age; (ii) stalk cells store lipids and exhibit thick walls, bladder cells present carotenes in small vesicles, oxalate crystals in vacuoles and lignin in their walls and both stalk and bladder cells have cuticles that difer in wax and cutin content; (iii) chloroplasts containing starch can be found on both stalk and bladder cells, and the latter also presents grana; (iv) plasmodesmata are observed between the stalk cell and the bladder cell, and between the epidermal cell and the stalk cell, and ectodesmata-like structures are observed on the bladder cell. Under high salinity conditions, (v) there is a clear tendency to accumulate greater amounts of K+ with respect to Na+ in the bladder cell; (vi) stalk cells accumulate similar amounts of K+ and Na+; (vii) Na+ accumulates mainly in the medullary parenchyma of the stem. These results add knowledge about the structure, content, and role of EBCc under salt stress, and surprisingly present the parenchyma of the stem as the main area of Na+ accumulation.
EEA San Juan
Fil: Palacios, María Belén. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Palacios, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rizzo, Alex Joel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Rizzo, Alex Joel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Heredia, Tatiana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Heredia, Tatiana Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Maldonado, Sara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Maldonado, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil. Murgida, Daniel Horacio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica Analítica y Química Física. INQUIMAE; Argentina
Fil: Burrieza, Hernán Pablo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Burrieza, Hernán Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Protoplasma : 1-15. (Published: 13 January 2024)
Materia
Chenopodium quinoa
Estrés Osmótico
Osmotic Strees
Quinoa
Quinua
Estrés Salino
Salt Stress
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/17381
Acceder
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oai_identifier_str oai:localhost:20.500.12123/17381
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stressPalacios, María BelénRizzo, Alex JoelHeredia, Tatiana BelénRoqueiro, GonzaloMaldonado, SaraMurgida, Daniel HoracioBurrieza, Hernán PabloChenopodium quinoaEstrés OsmóticoOsmotic StreesQuinoaQuinuaEstrés SalinoSalt StressQuinoa is a facultative halophyte with excellent tolerance to salinity. In this study, the epidermal bladder cell complex (EBCc) of quinoa leaves was studied to determine their cellular characteristics and involvement in salt tolerance. We used light microscopy, confocal RAMAN microscopy, confocal fuorescence microscopy, transmission electron microscopy, and environmental scanning electron microscopy complemented by energy dispersive X-ray analysis. Ionic content was quantifed with fame atomic absorption spectroscopy and with fame emission photometry. Results show that: (i) the number of EBCcs remains constant but their density and area vary with leaf age; (ii) stalk cells store lipids and exhibit thick walls, bladder cells present carotenes in small vesicles, oxalate crystals in vacuoles and lignin in their walls and both stalk and bladder cells have cuticles that difer in wax and cutin content; (iii) chloroplasts containing starch can be found on both stalk and bladder cells, and the latter also presents grana; (iv) plasmodesmata are observed between the stalk cell and the bladder cell, and between the epidermal cell and the stalk cell, and ectodesmata-like structures are observed on the bladder cell. Under high salinity conditions, (v) there is a clear tendency to accumulate greater amounts of K+ with respect to Na+ in the bladder cell; (vi) stalk cells accumulate similar amounts of K+ and Na+; (vii) Na+ accumulates mainly in the medullary parenchyma of the stem. These results add knowledge about the structure, content, and role of EBCc under salt stress, and surprisingly present the parenchyma of the stem as the main area of Na+ accumulation.EEA San JuanFil: Palacios, María Belén. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Palacios, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rizzo, Alex Joel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Rizzo, Alex Joel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Heredia, Tatiana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Heredia, Tatiana Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.Fil: Maldonado, Sara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Maldonado, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil. Murgida, Daniel Horacio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica Analítica y Química Física. INQUIMAE; ArgentinaFil: Burrieza, Hernán Pablo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; ArgentinaFil: Burrieza, Hernán Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2024-04-11T12:20:44Z2024-04-11T12:20:44Z2024-01-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/17381https://link.springer.com/article/10.1007/s00709-023-01922-x1615-61020033-183Xhttps://doi.org/10.1007/s00709-023-01922-xProtoplasma : 1-15. (Published: 13 January 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:27Zoai:localhost:20.500.12123/17381instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:27.88INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress
title Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress
spellingShingle Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress
Palacios, María Belén
Chenopodium quinoa
Estrés Osmótico
Osmotic Strees
Quinoa
Quinua
Estrés Salino
Salt Stress
title_short Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress
title_full Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress
title_fullStr Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress
title_full_unstemmed Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress
title_sort Structure, ultrastructure and cation accumulation in quinoa epidermal bladder cell complex under high saline stress
dc.creator.none.fl_str_mv Palacios, María Belén
Rizzo, Alex Joel
Heredia, Tatiana Belén
Roqueiro, Gonzalo
Maldonado, Sara
Murgida, Daniel Horacio
Burrieza, Hernán Pablo
author Palacios, María Belén
author_facet Palacios, María Belén
Rizzo, Alex Joel
Heredia, Tatiana Belén
Roqueiro, Gonzalo
Maldonado, Sara
Murgida, Daniel Horacio
Burrieza, Hernán Pablo
author_role author
author2 Rizzo, Alex Joel
Heredia, Tatiana Belén
Roqueiro, Gonzalo
Maldonado, Sara
Murgida, Daniel Horacio
Burrieza, Hernán Pablo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Chenopodium quinoa
Estrés Osmótico
Osmotic Strees
Quinoa
Quinua
Estrés Salino
Salt Stress
topic Chenopodium quinoa
Estrés Osmótico
Osmotic Strees
Quinoa
Quinua
Estrés Salino
Salt Stress
dc.description.none.fl_txt_mv Quinoa is a facultative halophyte with excellent tolerance to salinity. In this study, the epidermal bladder cell complex (EBCc) of quinoa leaves was studied to determine their cellular characteristics and involvement in salt tolerance. We used light microscopy, confocal RAMAN microscopy, confocal fuorescence microscopy, transmission electron microscopy, and environmental scanning electron microscopy complemented by energy dispersive X-ray analysis. Ionic content was quantifed with fame atomic absorption spectroscopy and with fame emission photometry. Results show that: (i) the number of EBCcs remains constant but their density and area vary with leaf age; (ii) stalk cells store lipids and exhibit thick walls, bladder cells present carotenes in small vesicles, oxalate crystals in vacuoles and lignin in their walls and both stalk and bladder cells have cuticles that difer in wax and cutin content; (iii) chloroplasts containing starch can be found on both stalk and bladder cells, and the latter also presents grana; (iv) plasmodesmata are observed between the stalk cell and the bladder cell, and between the epidermal cell and the stalk cell, and ectodesmata-like structures are observed on the bladder cell. Under high salinity conditions, (v) there is a clear tendency to accumulate greater amounts of K+ with respect to Na+ in the bladder cell; (vi) stalk cells accumulate similar amounts of K+ and Na+; (vii) Na+ accumulates mainly in the medullary parenchyma of the stem. These results add knowledge about the structure, content, and role of EBCc under salt stress, and surprisingly present the parenchyma of the stem as the main area of Na+ accumulation.
EEA San Juan
Fil: Palacios, María Belén. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Palacios, María Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rizzo, Alex Joel. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Rizzo, Alex Joel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Heredia, Tatiana Belén. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Heredia, Tatiana Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Roqueiro, Gonzalo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Juan; Argentina.
Fil: Maldonado, Sara. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Maldonado, Sara. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Murgida, Daniel Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil. Murgida, Daniel Horacio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Inorgánica Analítica y Química Física. INQUIMAE; Argentina
Fil: Burrieza, Hernán Pablo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Biodiversidad y Biología Experimental; Argentina
Fil: Burrieza, Hernán Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Quinoa is a facultative halophyte with excellent tolerance to salinity. In this study, the epidermal bladder cell complex (EBCc) of quinoa leaves was studied to determine their cellular characteristics and involvement in salt tolerance. We used light microscopy, confocal RAMAN microscopy, confocal fuorescence microscopy, transmission electron microscopy, and environmental scanning electron microscopy complemented by energy dispersive X-ray analysis. Ionic content was quantifed with fame atomic absorption spectroscopy and with fame emission photometry. Results show that: (i) the number of EBCcs remains constant but their density and area vary with leaf age; (ii) stalk cells store lipids and exhibit thick walls, bladder cells present carotenes in small vesicles, oxalate crystals in vacuoles and lignin in their walls and both stalk and bladder cells have cuticles that difer in wax and cutin content; (iii) chloroplasts containing starch can be found on both stalk and bladder cells, and the latter also presents grana; (iv) plasmodesmata are observed between the stalk cell and the bladder cell, and between the epidermal cell and the stalk cell, and ectodesmata-like structures are observed on the bladder cell. Under high salinity conditions, (v) there is a clear tendency to accumulate greater amounts of K+ with respect to Na+ in the bladder cell; (vi) stalk cells accumulate similar amounts of K+ and Na+; (vii) Na+ accumulates mainly in the medullary parenchyma of the stem. These results add knowledge about the structure, content, and role of EBCc under salt stress, and surprisingly present the parenchyma of the stem as the main area of Na+ accumulation.
publishDate 2024
dc.date.none.fl_str_mv 2024-04-11T12:20:44Z
2024-04-11T12:20:44Z
2024-01-13
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/17381
https://link.springer.com/article/10.1007/s00709-023-01922-x
1615-6102
0033-183X
https://doi.org/10.1007/s00709-023-01922-x
url http://hdl.handle.net/20.500.12123/17381
https://link.springer.com/article/10.1007/s00709-023-01922-x
https://doi.org/10.1007/s00709-023-01922-x
identifier_str_mv 1615-6102
0033-183X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Protoplasma : 1-15. (Published: 13 January 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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