High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation

Autores
Benavides-Silván, Julio; Katzer, Frank; Maley, Stephen W.; Bartley, Paul M.; Canton, German Jose; Palarea-Albaladejo, Javier; Purslow, Caroline A.; Pang, Yvonne; Rocchi, Mara; Chianini, Francesca; Buxton, David; Innes, Elisabeth A.
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In order to investigate the pathogenesis of neosporosis following a primary infection in late pregnancy, cattle were subcutaneously challenged with 5 × 108 Neospora caninum (NC1 isolate) tachyzoites at day 210 of gestation and serial necropsies were then carried out at 14, 28, 42 and 56 days post-infection (dpi). No abortions occurred and all the foetuses were viable at the time of euthanasia. There was a high rate of vertical transmission, as parasites were detected by immunohistochemical labelling and PCR in all the foetuses from 28 dpi. Focal necrotic lesions were observed in the placentomes of the placenta from 28 dpi and showed resolution during later time points, denoted by infiltration of inflammatory cells at 42 dpi and fibrosis at 56 dpi. Foetuses at 28 and 42 dpi showed scarce and isolated lesions which are unlikely to represent a threat to foetal viability. No lesions were observed in the foetuses at 14 or 56 dpi suggesting control of the infection and resolution of the lesions by maternal and foetal immune responses. Once infection was established, it could not be cleared from the host and vertical transmission of the parasite occurred in all infected hosts. Parasite was detected in the placenta at 28 dpi, while in previous experimental infections of cattle at day 70 and 140 of gestation using the same challenge model, it was already present at day 14 post infection. This suggests that a change in the maternal immune response plays a crucial role in limiting the initial infection during the last term of pregnancy.
EEA Balcarce
Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido
Fil: Purslow, Caroline A. Moredun Research Institute; Reino Unido
Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido
Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fil: Buxton, David. Moredun Research Institute; Reino Unido
Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido
Fuente
Veterinary Research 43 : 83 (2012)
Materia
Ganado Bovino
Enfermedades de los Animales
Neospora caninum
Gestación
Inmunidad Maternal
Respuesta Inmunológica
Cattle
Animal Diseases
Pregnancy
Maternal Immunity
Immune Response
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4366
Acceder
id INTADig_74608f142c775831324988ad3488ae6c
oai_identifier_str oai:localhost:20.500.12123/4366
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestationBenavides-Silván, JulioKatzer, FrankMaley, Stephen W.Bartley, Paul M.Canton, German JosePalarea-Albaladejo, JavierPurslow, Caroline A.Pang, YvonneRocchi, MaraChianini, FrancescaBuxton, DavidInnes, Elisabeth A.Ganado BovinoEnfermedades de los AnimalesNeospora caninumGestaciónInmunidad MaternalRespuesta InmunológicaCattleAnimal DiseasesPregnancyMaternal ImmunityImmune ResponseIn order to investigate the pathogenesis of neosporosis following a primary infection in late pregnancy, cattle were subcutaneously challenged with 5 × 108 Neospora caninum (NC1 isolate) tachyzoites at day 210 of gestation and serial necropsies were then carried out at 14, 28, 42 and 56 days post-infection (dpi). No abortions occurred and all the foetuses were viable at the time of euthanasia. There was a high rate of vertical transmission, as parasites were detected by immunohistochemical labelling and PCR in all the foetuses from 28 dpi. Focal necrotic lesions were observed in the placentomes of the placenta from 28 dpi and showed resolution during later time points, denoted by infiltration of inflammatory cells at 42 dpi and fibrosis at 56 dpi. Foetuses at 28 and 42 dpi showed scarce and isolated lesions which are unlikely to represent a threat to foetal viability. No lesions were observed in the foetuses at 14 or 56 dpi suggesting control of the infection and resolution of the lesions by maternal and foetal immune responses. Once infection was established, it could not be cleared from the host and vertical transmission of the parasite occurred in all infected hosts. Parasite was detected in the placenta at 28 dpi, while in previous experimental infections of cattle at day 70 and 140 of gestation using the same challenge model, it was already present at day 14 post infection. This suggests that a change in the maternal immune response plays a crucial role in limiting the initial infection during the last term of pregnancy.EEA BalcarceFil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; EspañaFil: Katzer, Frank. Moredun Research Institute; Reino UnidoFil: Maley, Stephen W. Moredun Research Institute; Reino UnidoFil: Bartley, Paul M. Moredun Research Institute; Reino UnidoFil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino UnidoFil: Purslow, Caroline A. Moredun Research Institute; Reino UnidoFil: Pang, Yvonne. Moredun Research Institute; Reino UnidoFil: Rocchi, Mara. Moredun Research Institute; Reino UnidoFil: Chianini, Francesca. Moredun Research Institute; Reino UnidoFil: Buxton, David. Moredun Research Institute; Reino UnidoFil: Innes, Elisabeth A. Moredun Research Institute; Reino UnidoBMC2019-01-31T13:16:29Z2019-01-31T13:16:29Z2012-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-43-83http://hdl.handle.net/20.500.12123/43660928-42491297-9716https://doi.org/10.1186/1297-9716-43-83Veterinary Research 43 : 83 (2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:16:49Zoai:localhost:20.500.12123/4366instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:49.421INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation
title High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation
spellingShingle High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation
Benavides-Silván, Julio
Ganado Bovino
Enfermedades de los Animales
Neospora caninum
Gestación
Inmunidad Maternal
Respuesta Inmunológica
Cattle
Animal Diseases
Pregnancy
Maternal Immunity
Immune Response
title_short High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation
title_full High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation
title_fullStr High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation
title_full_unstemmed High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation
title_sort High rate of transplacental infection and transmission of Neospora caninum following experimental challenge of cattle at day 210 of gestation
dc.creator.none.fl_str_mv Benavides-Silván, Julio
Katzer, Frank
Maley, Stephen W.
Bartley, Paul M.
Canton, German Jose
Palarea-Albaladejo, Javier
Purslow, Caroline A.
Pang, Yvonne
Rocchi, Mara
Chianini, Francesca
Buxton, David
Innes, Elisabeth A.
author Benavides-Silván, Julio
author_facet Benavides-Silván, Julio
Katzer, Frank
Maley, Stephen W.
Bartley, Paul M.
Canton, German Jose
Palarea-Albaladejo, Javier
Purslow, Caroline A.
Pang, Yvonne
Rocchi, Mara
Chianini, Francesca
Buxton, David
Innes, Elisabeth A.
author_role author
author2 Katzer, Frank
Maley, Stephen W.
Bartley, Paul M.
Canton, German Jose
Palarea-Albaladejo, Javier
Purslow, Caroline A.
Pang, Yvonne
Rocchi, Mara
Chianini, Francesca
Buxton, David
Innes, Elisabeth A.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Enfermedades de los Animales
Neospora caninum
Gestación
Inmunidad Maternal
Respuesta Inmunológica
Cattle
Animal Diseases
Pregnancy
Maternal Immunity
Immune Response
topic Ganado Bovino
Enfermedades de los Animales
Neospora caninum
Gestación
Inmunidad Maternal
Respuesta Inmunológica
Cattle
Animal Diseases
Pregnancy
Maternal Immunity
Immune Response
dc.description.none.fl_txt_mv In order to investigate the pathogenesis of neosporosis following a primary infection in late pregnancy, cattle were subcutaneously challenged with 5 × 108 Neospora caninum (NC1 isolate) tachyzoites at day 210 of gestation and serial necropsies were then carried out at 14, 28, 42 and 56 days post-infection (dpi). No abortions occurred and all the foetuses were viable at the time of euthanasia. There was a high rate of vertical transmission, as parasites were detected by immunohistochemical labelling and PCR in all the foetuses from 28 dpi. Focal necrotic lesions were observed in the placentomes of the placenta from 28 dpi and showed resolution during later time points, denoted by infiltration of inflammatory cells at 42 dpi and fibrosis at 56 dpi. Foetuses at 28 and 42 dpi showed scarce and isolated lesions which are unlikely to represent a threat to foetal viability. No lesions were observed in the foetuses at 14 or 56 dpi suggesting control of the infection and resolution of the lesions by maternal and foetal immune responses. Once infection was established, it could not be cleared from the host and vertical transmission of the parasite occurred in all infected hosts. Parasite was detected in the placenta at 28 dpi, while in previous experimental infections of cattle at day 70 and 140 of gestation using the same challenge model, it was already present at day 14 post infection. This suggests that a change in the maternal immune response plays a crucial role in limiting the initial infection during the last term of pregnancy.
EEA Balcarce
Fil: Benavides-Silván, Julio. Moredun Research Institute; Reino Unido. Consejo Superior de Investigaciones Científicas. Instituto de Ganadería de Montaña; España
Fil: Katzer, Frank. Moredun Research Institute; Reino Unido
Fil: Maley, Stephen W. Moredun Research Institute; Reino Unido
Fil: Bartley, Paul M. Moredun Research Institute; Reino Unido
Fil: Canton, German Jose. Moredun Research Institute; Reino Unido. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Palarea-Albaladejo, Javier. Biomathematics & Statistics Scotland; Reino Unido
Fil: Purslow, Caroline A. Moredun Research Institute; Reino Unido
Fil: Pang, Yvonne. Moredun Research Institute; Reino Unido
Fil: Rocchi, Mara. Moredun Research Institute; Reino Unido
Fil: Chianini, Francesca. Moredun Research Institute; Reino Unido
Fil: Buxton, David. Moredun Research Institute; Reino Unido
Fil: Innes, Elisabeth A. Moredun Research Institute; Reino Unido
description In order to investigate the pathogenesis of neosporosis following a primary infection in late pregnancy, cattle were subcutaneously challenged with 5 × 108 Neospora caninum (NC1 isolate) tachyzoites at day 210 of gestation and serial necropsies were then carried out at 14, 28, 42 and 56 days post-infection (dpi). No abortions occurred and all the foetuses were viable at the time of euthanasia. There was a high rate of vertical transmission, as parasites were detected by immunohistochemical labelling and PCR in all the foetuses from 28 dpi. Focal necrotic lesions were observed in the placentomes of the placenta from 28 dpi and showed resolution during later time points, denoted by infiltration of inflammatory cells at 42 dpi and fibrosis at 56 dpi. Foetuses at 28 and 42 dpi showed scarce and isolated lesions which are unlikely to represent a threat to foetal viability. No lesions were observed in the foetuses at 14 or 56 dpi suggesting control of the infection and resolution of the lesions by maternal and foetal immune responses. Once infection was established, it could not be cleared from the host and vertical transmission of the parasite occurred in all infected hosts. Parasite was detected in the placenta at 28 dpi, while in previous experimental infections of cattle at day 70 and 140 of gestation using the same challenge model, it was already present at day 14 post infection. This suggests that a change in the maternal immune response plays a crucial role in limiting the initial infection during the last term of pregnancy.
publishDate 2012
dc.date.none.fl_str_mv 2012-12
2019-01-31T13:16:29Z
2019-01-31T13:16:29Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-43-83
http://hdl.handle.net/20.500.12123/4366
0928-4249
1297-9716
https://doi.org/10.1186/1297-9716-43-83
url https://veterinaryresearch.biomedcentral.com/articles/10.1186/1297-9716-43-83
http://hdl.handle.net/20.500.12123/4366
https://doi.org/10.1186/1297-9716-43-83
identifier_str_mv 0928-4249
1297-9716
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv Veterinary Research 43 : 83 (2012)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846787517988208640
score 12.982451

Publicaciones similares