Exploring the cellulolytic activity of environmental mycobacteria

Autores
Mon, Maria Laura; Romano, Nelson Gaston; Farace, Pablo Daniel; Tortone, Claudia Andrea; Oriani, Delia Susana; Picariello, Gianluca; Zumarraga, Martin Jose; Gioffre, Andrea; Talia, Paola Mónica
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although studies on non-tuberculous mycobacteria have increased in recent years because they cause a considerable proportion of infections, their cellulolytic system is still poorly studied. This study presents a characterization of the cellulolytic activities of environmental mycobacterial isolates derived from soil and water samples from the central region of Argentina, aimed to evaluate the conservation of the mechanism for the degradation of cellulose in this group of bacteria. The molecular and genomic identification revealed identity with Mycolicibacterium septicum. The endoglucanase and total cellulase activities were assessed both qualitatively and quantitatively and the optimal enzymatic conditions were characterized. A specific protein of around 56 kDa with cellulolytic activity was detected in a zymogram. Protein sequences possibly arising from a cellulase were identified by mass spectrometry-based shotgun proteomics. Results showed that M. septicum encodes for cellulose- and hemicellulose-related degrading enzymes, including at least an active β-1,4 endoglucanase enzyme that could be useful to improve its survival in the environment. Given the important health issues related to mycobacteria, the results of the present study may contribute to the knowledge of their cellulolytic system, which could be important for their ability to survive in many different types of environments.
Instituto de Biotecnología
Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romano, Nelson Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Romano, Nelson Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Farace, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Farace, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias. Cátedra de Bacteriología y Micología; Argentina
Fil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias. Cátedra de Bacteriología y Micología; Argentina
Fil: Picariello, Gianluca. Consiglio Nazionale Delle Ricerche. Istituto di Scienze Dell’Alimentazione; Italia
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gioffre, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Gioffre, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Tuberculosis 147 : 102516 (July 2024)
Materia
Cellulase
Cellulolytic Microorganisms
Celulasa
Microorganismos Celulolíticos
Mycolicibacterium septicum
Micobacterias Ambientales
Endoglucanasa Activa
Environmental Mycobacteria
Active Endoglucanase
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/17730
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oai_identifier_str oai:localhost:20.500.12123/17730
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network_name_str INTA Digital (INTA)
spelling Exploring the cellulolytic activity of environmental mycobacteriaMon, Maria LauraRomano, Nelson GastonFarace, Pablo DanielTortone, Claudia AndreaOriani, Delia SusanaPicariello, GianlucaZumarraga, Martin JoseGioffre, AndreaTalia, Paola MónicaCellulaseCellulolytic MicroorganismsCelulasaMicroorganismos CelulolíticosMycolicibacterium septicumMicobacterias AmbientalesEndoglucanasa ActivaEnvironmental MycobacteriaActive EndoglucanaseAlthough studies on non-tuberculous mycobacteria have increased in recent years because they cause a considerable proportion of infections, their cellulolytic system is still poorly studied. This study presents a characterization of the cellulolytic activities of environmental mycobacterial isolates derived from soil and water samples from the central region of Argentina, aimed to evaluate the conservation of the mechanism for the degradation of cellulose in this group of bacteria. The molecular and genomic identification revealed identity with Mycolicibacterium septicum. The endoglucanase and total cellulase activities were assessed both qualitatively and quantitatively and the optimal enzymatic conditions were characterized. A specific protein of around 56 kDa with cellulolytic activity was detected in a zymogram. Protein sequences possibly arising from a cellulase were identified by mass spectrometry-based shotgun proteomics. Results showed that M. septicum encodes for cellulose- and hemicellulose-related degrading enzymes, including at least an active β-1,4 endoglucanase enzyme that could be useful to improve its survival in the environment. Given the important health issues related to mycobacteria, the results of the present study may contribute to the knowledge of their cellulolytic system, which could be important for their ability to survive in many different types of environments.Instituto de BiotecnologíaFil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romano, Nelson Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Romano, Nelson Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Farace, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Farace, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias. Cátedra de Bacteriología y Micología; ArgentinaFil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias. Cátedra de Bacteriología y Micología; ArgentinaFil: Picariello, Gianluca. Consiglio Nazionale Delle Ricerche. Istituto di Scienze Dell’Alimentazione; ItaliaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gioffre, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Gioffre, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2024-05-14T17:55:15Z2024-05-14T17:55:15Z2024-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/17730https://www.sciencedirect.com/science/article/abs/pii/S14729792240004281472-9792https://doi.org/10.1016/j.tube.2024.102516Tuberculosis 147 : 102516 (July 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I085, Identificación y caracterización funcional de genes interés biotecnológico para la sostenibilidad productiva y ambientalinfo:eu-repograntAgreement/INTA/2023-PD-L01-I089, Microbiomas en ecosistemas agropecuarios: la conexión integradora del enfoque Una Saludinfo:eu-repograntAgreement/INTA/2023-PD-L01-I102, Bases ecofisiológicas para mejorar la adaptación de los cultivos a la variabilidad ambiental mediante el manejo agronómico y el mejoramiento genéticoinfo:eu-repograntAgreement/INTA/2023-PD-L04-I122, Gestión de las biomasas del SAB y estrategias tecnológicas para su transformación en bioproductos de valor agregadoinfo:eu-repograntAgreement/INTA/2019-PT-E7-I159-001, Info e innovación p/ VA, agroind. y bioenergíainfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:31Zoai:localhost:20.500.12123/17730instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:32.27INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Exploring the cellulolytic activity of environmental mycobacteria
title Exploring the cellulolytic activity of environmental mycobacteria
spellingShingle Exploring the cellulolytic activity of environmental mycobacteria
Mon, Maria Laura
Cellulase
Cellulolytic Microorganisms
Celulasa
Microorganismos Celulolíticos
Mycolicibacterium septicum
Micobacterias Ambientales
Endoglucanasa Activa
Environmental Mycobacteria
Active Endoglucanase
title_short Exploring the cellulolytic activity of environmental mycobacteria
title_full Exploring the cellulolytic activity of environmental mycobacteria
title_fullStr Exploring the cellulolytic activity of environmental mycobacteria
title_full_unstemmed Exploring the cellulolytic activity of environmental mycobacteria
title_sort Exploring the cellulolytic activity of environmental mycobacteria
dc.creator.none.fl_str_mv Mon, Maria Laura
Romano, Nelson Gaston
Farace, Pablo Daniel
Tortone, Claudia Andrea
Oriani, Delia Susana
Picariello, Gianluca
Zumarraga, Martin Jose
Gioffre, Andrea
Talia, Paola Mónica
author Mon, Maria Laura
author_facet Mon, Maria Laura
Romano, Nelson Gaston
Farace, Pablo Daniel
Tortone, Claudia Andrea
Oriani, Delia Susana
Picariello, Gianluca
Zumarraga, Martin Jose
Gioffre, Andrea
Talia, Paola Mónica
author_role author
author2 Romano, Nelson Gaston
Farace, Pablo Daniel
Tortone, Claudia Andrea
Oriani, Delia Susana
Picariello, Gianluca
Zumarraga, Martin Jose
Gioffre, Andrea
Talia, Paola Mónica
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cellulase
Cellulolytic Microorganisms
Celulasa
Microorganismos Celulolíticos
Mycolicibacterium septicum
Micobacterias Ambientales
Endoglucanasa Activa
Environmental Mycobacteria
Active Endoglucanase
topic Cellulase
Cellulolytic Microorganisms
Celulasa
Microorganismos Celulolíticos
Mycolicibacterium septicum
Micobacterias Ambientales
Endoglucanasa Activa
Environmental Mycobacteria
Active Endoglucanase
dc.description.none.fl_txt_mv Although studies on non-tuberculous mycobacteria have increased in recent years because they cause a considerable proportion of infections, their cellulolytic system is still poorly studied. This study presents a characterization of the cellulolytic activities of environmental mycobacterial isolates derived from soil and water samples from the central region of Argentina, aimed to evaluate the conservation of the mechanism for the degradation of cellulose in this group of bacteria. The molecular and genomic identification revealed identity with Mycolicibacterium septicum. The endoglucanase and total cellulase activities were assessed both qualitatively and quantitatively and the optimal enzymatic conditions were characterized. A specific protein of around 56 kDa with cellulolytic activity was detected in a zymogram. Protein sequences possibly arising from a cellulase were identified by mass spectrometry-based shotgun proteomics. Results showed that M. septicum encodes for cellulose- and hemicellulose-related degrading enzymes, including at least an active β-1,4 endoglucanase enzyme that could be useful to improve its survival in the environment. Given the important health issues related to mycobacteria, the results of the present study may contribute to the knowledge of their cellulolytic system, which could be important for their ability to survive in many different types of environments.
Instituto de Biotecnología
Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romano, Nelson Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Romano, Nelson Gaston. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Farace, Pablo Daniel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Farace, Pablo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tortone, Claudia Andrea. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias. Cátedra de Bacteriología y Micología; Argentina
Fil: Oriani, Delia Susana. Universidad Nacional de La Pampa. Facultad de Ciencias Veterinarias. Cátedra de Bacteriología y Micología; Argentina
Fil: Picariello, Gianluca. Consiglio Nazionale Delle Ricerche. Istituto di Scienze Dell’Alimentazione; Italia
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gioffre, Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Gioffre, Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Talia, Paola Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Although studies on non-tuberculous mycobacteria have increased in recent years because they cause a considerable proportion of infections, their cellulolytic system is still poorly studied. This study presents a characterization of the cellulolytic activities of environmental mycobacterial isolates derived from soil and water samples from the central region of Argentina, aimed to evaluate the conservation of the mechanism for the degradation of cellulose in this group of bacteria. The molecular and genomic identification revealed identity with Mycolicibacterium septicum. The endoglucanase and total cellulase activities were assessed both qualitatively and quantitatively and the optimal enzymatic conditions were characterized. A specific protein of around 56 kDa with cellulolytic activity was detected in a zymogram. Protein sequences possibly arising from a cellulase were identified by mass spectrometry-based shotgun proteomics. Results showed that M. septicum encodes for cellulose- and hemicellulose-related degrading enzymes, including at least an active β-1,4 endoglucanase enzyme that could be useful to improve its survival in the environment. Given the important health issues related to mycobacteria, the results of the present study may contribute to the knowledge of their cellulolytic system, which could be important for their ability to survive in many different types of environments.
publishDate 2024
dc.date.none.fl_str_mv 2024-05-14T17:55:15Z
2024-05-14T17:55:15Z
2024-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/17730
https://www.sciencedirect.com/science/article/abs/pii/S1472979224000428
1472-9792
https://doi.org/10.1016/j.tube.2024.102516
url http://hdl.handle.net/20.500.12123/17730
https://www.sciencedirect.com/science/article/abs/pii/S1472979224000428
https://doi.org/10.1016/j.tube.2024.102516
identifier_str_mv 1472-9792
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I085, Identificación y caracterización funcional de genes interés biotecnológico para la sostenibilidad productiva y ambiental
info:eu-repograntAgreement/INTA/2023-PD-L01-I089, Microbiomas en ecosistemas agropecuarios: la conexión integradora del enfoque Una Salud
info:eu-repograntAgreement/INTA/2023-PD-L01-I102, Bases ecofisiológicas para mejorar la adaptación de los cultivos a la variabilidad ambiental mediante el manejo agronómico y el mejoramiento genético
info:eu-repograntAgreement/INTA/2023-PD-L04-I122, Gestión de las biomasas del SAB y estrategias tecnológicas para su transformación en bioproductos de valor agregado
info:eu-repograntAgreement/INTA/2019-PT-E7-I159-001, Info e innovación p/ VA, agroind. y bioenergía
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Tuberculosis 147 : 102516 (July 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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