An economical and high throughput alternative for endoglucanase activity determination

Autores
Ortiz, Gastón Ezequiel; Blasco, Martín; Alberto, Edgardo Omar
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The search for new strains with good productivity of cellulolytic enzymes is always necessary to improve the processes are usually employed. Screening protocols used for these purposes require a low cost and high throughput assay for determination of endoglucanase activity. However, the test proposed by the IUPAC and other microadaptations not meet this requirement. The aim of our work is to obtain an economical and high-performance proposed by IUPAC for determination of endoglucanase activity assay adaptation. For this we reduced the assay volume and investigate the effect of this reduction on endoglucanase activity determination. As a result a miniaturized assay statistically correlated with the assay proposed by IUPAC reduced 10-fold lower scale was obtained. As a result a new economic and high performance adaptation for endoglucanase activity assay based on the IUPAC protocol was obtained.
Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Blasco, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo en Biotecnología Industrial; Argentina
Fil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Materia
Miniaturized CMCase assay
Cellulase
Endoglucanase
Carboxymethylcellulases
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/78156
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An economical and high throughput alternative for endoglucanase activity determinationOrtiz, Gastón EzequielBlasco, MartínAlberto, Edgardo OmarMiniaturized CMCase assayCellulaseEndoglucanaseCarboxymethylcellulaseshttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2The search for new strains with good productivity of cellulolytic enzymes is always necessary to improve the processes are usually employed. Screening protocols used for these purposes require a low cost and high throughput assay for determination of endoglucanase activity. However, the test proposed by the IUPAC and other microadaptations not meet this requirement. The aim of our work is to obtain an economical and high-performance proposed by IUPAC for determination of endoglucanase activity assay adaptation. For this we reduced the assay volume and investigate the effect of this reduction on endoglucanase activity determination. As a result a miniaturized assay statistically correlated with the assay proposed by IUPAC reduced 10-fold lower scale was obtained. As a result a new economic and high performance adaptation for endoglucanase activity assay based on the IUPAC protocol was obtained.Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaFil: Blasco, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo en Biotecnología Industrial; ArgentinaFil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); ArgentinaTrade Science2016-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/78156Ortiz, Gastón Ezequiel; Blasco, Martín; Alberto, Edgardo Omar; An economical and high throughput alternative for endoglucanase activity determination; Trade Science; BioTechnology; 12; 2; 1-2016; 70-740974-7435CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.tsijournals.com/journals/archive/tsbt-volume-12-issue-2-year-2016.htmlinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:22:41Zoai:ri.conicet.gov.ar:11336/78156instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:22:41.418CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An economical and high throughput alternative for endoglucanase activity determination
title An economical and high throughput alternative for endoglucanase activity determination
spellingShingle An economical and high throughput alternative for endoglucanase activity determination
Ortiz, Gastón Ezequiel
Miniaturized CMCase assay
Cellulase
Endoglucanase
Carboxymethylcellulases
title_short An economical and high throughput alternative for endoglucanase activity determination
title_full An economical and high throughput alternative for endoglucanase activity determination
title_fullStr An economical and high throughput alternative for endoglucanase activity determination
title_full_unstemmed An economical and high throughput alternative for endoglucanase activity determination
title_sort An economical and high throughput alternative for endoglucanase activity determination
dc.creator.none.fl_str_mv Ortiz, Gastón Ezequiel
Blasco, Martín
Alberto, Edgardo Omar
author Ortiz, Gastón Ezequiel
author_facet Ortiz, Gastón Ezequiel
Blasco, Martín
Alberto, Edgardo Omar
author_role author
author2 Blasco, Martín
Alberto, Edgardo Omar
author2_role author
author
dc.subject.none.fl_str_mv Miniaturized CMCase assay
Cellulase
Endoglucanase
Carboxymethylcellulases
topic Miniaturized CMCase assay
Cellulase
Endoglucanase
Carboxymethylcellulases
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv The search for new strains with good productivity of cellulolytic enzymes is always necessary to improve the processes are usually employed. Screening protocols used for these purposes require a low cost and high throughput assay for determination of endoglucanase activity. However, the test proposed by the IUPAC and other microadaptations not meet this requirement. The aim of our work is to obtain an economical and high-performance proposed by IUPAC for determination of endoglucanase activity assay adaptation. For this we reduced the assay volume and investigate the effect of this reduction on endoglucanase activity determination. As a result a miniaturized assay statistically correlated with the assay proposed by IUPAC reduced 10-fold lower scale was obtained. As a result a new economic and high performance adaptation for endoglucanase activity assay based on the IUPAC protocol was obtained.
Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
Fil: Blasco, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo en Biotecnología Industrial; Argentina
Fil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina
description The search for new strains with good productivity of cellulolytic enzymes is always necessary to improve the processes are usually employed. Screening protocols used for these purposes require a low cost and high throughput assay for determination of endoglucanase activity. However, the test proposed by the IUPAC and other microadaptations not meet this requirement. The aim of our work is to obtain an economical and high-performance proposed by IUPAC for determination of endoglucanase activity assay adaptation. For this we reduced the assay volume and investigate the effect of this reduction on endoglucanase activity determination. As a result a miniaturized assay statistically correlated with the assay proposed by IUPAC reduced 10-fold lower scale was obtained. As a result a new economic and high performance adaptation for endoglucanase activity assay based on the IUPAC protocol was obtained.
publishDate 2016
dc.date.none.fl_str_mv 2016-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/78156
Ortiz, Gastón Ezequiel; Blasco, Martín; Alberto, Edgardo Omar; An economical and high throughput alternative for endoglucanase activity determination; Trade Science; BioTechnology; 12; 2; 1-2016; 70-74
0974-7435
CONICET Digital
CONICET
url http://hdl.handle.net/11336/78156
identifier_str_mv Ortiz, Gastón Ezequiel; Blasco, Martín; Alberto, Edgardo Omar; An economical and high throughput alternative for endoglucanase activity determination; Trade Science; BioTechnology; 12; 2; 1-2016; 70-74
0974-7435
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.tsijournals.com/journals/archive/tsbt-volume-12-issue-2-year-2016.html
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Trade Science
publisher.none.fl_str_mv Trade Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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