Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest

Autores
Romano, Nelson Gaston; Gioffre, Andrea; Sede, Silvana Mabel; Campos, Eleonora; Cataldi, Angel Adrian; Talia, Paola Mónica
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups including Achromobacter and Pseudomonas genera. All three consortia presented cellulolytic activity. The carboxymethylcellulase (CMCase) and total cellulase activities were studied both quantitatively and qualitatively and optimal enzymatic conditions were characterized and compared among the three consortia. Thermal and pH stability were analyzed. Based on its cellulolytic activity, one consortium was selected for further characterization by zymography. We detected a specific protein of 55 kDa with CMCase activity. In this study, we have shown that these consortia encode for cellulolytic enzymes. These enzymes could be useful for lignocellulosic biomass degradation into simple components and for different industrial applications.
Instituto de Biotecnología
Fil: Romano, Nelson Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Sede, Silvana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Current Microbiology 67 (2) : 138–147 (August 2013)
Materia
Bosques
Bosque Primario
Pseudomonas
Celulasa
Actividad Enzimática
Forests
Primary Forests
Cellulase
Enzyme Activity
Bosques Nativos
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4272
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4272
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network_name_str INTA Digital (INTA)
spelling Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native ForestRomano, Nelson GastonGioffre, AndreaSede, Silvana MabelCampos, EleonoraCataldi, Angel AdrianTalia, Paola MónicaBosquesBosque PrimarioPseudomonasCelulasaActividad EnzimáticaForestsPrimary ForestsCellulaseEnzyme ActivityBosques NativosArgentinaCellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups including Achromobacter and Pseudomonas genera. All three consortia presented cellulolytic activity. The carboxymethylcellulase (CMCase) and total cellulase activities were studied both quantitatively and qualitatively and optimal enzymatic conditions were characterized and compared among the three consortia. Thermal and pH stability were analyzed. Based on its cellulolytic activity, one consortium was selected for further characterization by zymography. We detected a specific protein of 55 kDa with CMCase activity. In this study, we have shown that these consortia encode for cellulolytic enzymes. These enzymes could be useful for lignocellulosic biomass degradation into simple components and for different industrial applications.Instituto de BiotecnologíaFil: Romano, Nelson Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.Fil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Sede, Silvana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; ArgentinaFil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaSpringer2019-01-15T17:55:21Z2019-01-15T17:55:21Z2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007%2Fs00284-013-0345-2http://hdl.handle.net/20.500.12123/42720343-86511432-0991https://doi.org/10.1007/s00284-013-0345-2Current Microbiology 67 (2) : 138–147 (August 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:33Zoai:localhost:20.500.12123/4272instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:33.443INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
title Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
spellingShingle Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
Romano, Nelson Gaston
Bosques
Bosque Primario
Pseudomonas
Celulasa
Actividad Enzimática
Forests
Primary Forests
Cellulase
Enzyme Activity
Bosques Nativos
Argentina
title_short Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
title_full Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
title_fullStr Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
title_full_unstemmed Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
title_sort Characterization of Cellulolytic Activities of Environmental Bacterial Consortia from an Argentinian Native Forest
dc.creator.none.fl_str_mv Romano, Nelson Gaston
Gioffre, Andrea
Sede, Silvana Mabel
Campos, Eleonora
Cataldi, Angel Adrian
Talia, Paola Mónica
author Romano, Nelson Gaston
author_facet Romano, Nelson Gaston
Gioffre, Andrea
Sede, Silvana Mabel
Campos, Eleonora
Cataldi, Angel Adrian
Talia, Paola Mónica
author_role author
author2 Gioffre, Andrea
Sede, Silvana Mabel
Campos, Eleonora
Cataldi, Angel Adrian
Talia, Paola Mónica
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Bosques
Bosque Primario
Pseudomonas
Celulasa
Actividad Enzimática
Forests
Primary Forests
Cellulase
Enzyme Activity
Bosques Nativos
Argentina
topic Bosques
Bosque Primario
Pseudomonas
Celulasa
Actividad Enzimática
Forests
Primary Forests
Cellulase
Enzyme Activity
Bosques Nativos
Argentina
dc.description.none.fl_txt_mv Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups including Achromobacter and Pseudomonas genera. All three consortia presented cellulolytic activity. The carboxymethylcellulase (CMCase) and total cellulase activities were studied both quantitatively and qualitatively and optimal enzymatic conditions were characterized and compared among the three consortia. Thermal and pH stability were analyzed. Based on its cellulolytic activity, one consortium was selected for further characterization by zymography. We detected a specific protein of 55 kDa with CMCase activity. In this study, we have shown that these consortia encode for cellulolytic enzymes. These enzymes could be useful for lignocellulosic biomass degradation into simple components and for different industrial applications.
Instituto de Biotecnología
Fil: Romano, Nelson Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Gioffre, Andrea Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Sede, Silvana Mabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica Darwinion. Academia Nacional de Ciencias Exactas, Físicas y Naturales. Instituto de Botánica Darwinion; Argentina
Fil: Campos, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Talia, Paola Mónica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Cellulolytic activities of three bacterial consortia derived from a forest soil sample from Chaco region, Argentina, were characterized. The phylogenetic analysis of consortia revealed two main highly supported groups including Achromobacter and Pseudomonas genera. All three consortia presented cellulolytic activity. The carboxymethylcellulase (CMCase) and total cellulase activities were studied both quantitatively and qualitatively and optimal enzymatic conditions were characterized and compared among the three consortia. Thermal and pH stability were analyzed. Based on its cellulolytic activity, one consortium was selected for further characterization by zymography. We detected a specific protein of 55 kDa with CMCase activity. In this study, we have shown that these consortia encode for cellulolytic enzymes. These enzymes could be useful for lignocellulosic biomass degradation into simple components and for different industrial applications.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
2019-01-15T17:55:21Z
2019-01-15T17:55:21Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007%2Fs00284-013-0345-2
http://hdl.handle.net/20.500.12123/4272
0343-8651
1432-0991
https://doi.org/10.1007/s00284-013-0345-2
url https://link.springer.com/article/10.1007%2Fs00284-013-0345-2
http://hdl.handle.net/20.500.12123/4272
https://doi.org/10.1007/s00284-013-0345-2
identifier_str_mv 0343-8651
1432-0991
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Current Microbiology 67 (2) : 138–147 (August 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619130029735936
score 12.558318

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