Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruits

Autores
Dominguez, Pia Guadalupe; Conti, Gabriela; Duffy, Tomas; Insani, Ester Marina; Alseekh, Saleh; Asurmendi, Sebastian; Fernie, Alisdair R.; Carrari, Fernando
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The transcription factor ASR1 (ABA, STRESS, RIPENING 1) plays multiple roles in plant responses to abiotic stresses as well as being involved in the regulation of central metabolism in several plant species. However, despite the high expression of ASR1 in tomato fruits, large scale analyses to uncover its function in fruits are still lacking. In order to study its function in the context of fruit ripening, we performed a multiomics analysis of ASR1-antisense transgenic tomato fruits at the transcriptome and metabolome levels. Our results indicate that ASR1 is involved in several pathways implicated in the fruit ripening process, including cell wall, amino acid, and carotenoid metabolism, as well as abiotic stress pathways. Moreover, we found that ASR1-antisense fruits are more susceptible to the infection by the necrotrophic fungus Botrytis cinerea. Given that ASR1 could be regulated by fruit ripening regulators such as FRUITFULL1/FRUITFULL2 (FUL1/FUL2), NON-RIPENING (NOR), and COLORLESS NON-RIPENING (CNR), we positioned it in the regulatory cascade of red ripe tomato fruits. These data extend the known range of functions of ASR1 as an important auxiliary regulator of tomato fruit ripening.
Instituto de Biotecnología
Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Dominguez, Pia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Conti, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conti, Gabriela. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Duffy, Tomás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Duffy, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Insani, Ester Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Insani, Ester Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alseekh, Saleh. Max-Planck-Institute of Molecular Plant Physiology; Alemania
Fil: Alseekh, Saleh. Center of Plant Systems Biology and Biotechnology; Bulgaria
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernie, Alisdair R. Max-Planck-Institute of Molecular Plant Physiology; Alemania
Fil: Fernie, Alisdair R. Center of Plant Systems Biology and Biotechnology; Bulgaria
Fil: Carrari, Fernando. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Carrari, Fernando. Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of Experimental Botany 72 (18) : 6490-6509 (Septiembre 2021)
Materia
Botrytis cinerea
Metabolómica
Transcriptómica
Tomate
Estrés Abiótico
Maduramiento
Metabolomics
Transcriptomics
Tomatoes
ABA
Abiotic Stress
Ripening
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16535
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oai_identifier_str oai:localhost:20.500.12123/16535
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruitsDominguez, Pia GuadalupeConti, GabrielaDuffy, TomasInsani, Ester MarinaAlseekh, SalehAsurmendi, SebastianFernie, Alisdair R.Carrari, FernandoBotrytis cinereaMetabolómicaTranscriptómicaTomateEstrés AbióticoMaduramientoMetabolomicsTranscriptomicsTomatoesABAAbiotic StressRipeningThe transcription factor ASR1 (ABA, STRESS, RIPENING 1) plays multiple roles in plant responses to abiotic stresses as well as being involved in the regulation of central metabolism in several plant species. However, despite the high expression of ASR1 in tomato fruits, large scale analyses to uncover its function in fruits are still lacking. In order to study its function in the context of fruit ripening, we performed a multiomics analysis of ASR1-antisense transgenic tomato fruits at the transcriptome and metabolome levels. Our results indicate that ASR1 is involved in several pathways implicated in the fruit ripening process, including cell wall, amino acid, and carotenoid metabolism, as well as abiotic stress pathways. Moreover, we found that ASR1-antisense fruits are more susceptible to the infection by the necrotrophic fungus Botrytis cinerea. Given that ASR1 could be regulated by fruit ripening regulators such as FRUITFULL1/FRUITFULL2 (FUL1/FUL2), NON-RIPENING (NOR), and COLORLESS NON-RIPENING (CNR), we positioned it in the regulatory cascade of red ripe tomato fruits. These data extend the known range of functions of ASR1 as an important auxiliary regulator of tomato fruit ripening.Instituto de BiotecnologíaFil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Dominguez, Pia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Conti, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Conti, Gabriela. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Duffy, Tomás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Duffy, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Insani, Ester Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Insani, Ester Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Alseekh, Saleh. Max-Planck-Institute of Molecular Plant Physiology; AlemaniaFil: Alseekh, Saleh. Center of Plant Systems Biology and Biotechnology; BulgariaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernie, Alisdair R. Max-Planck-Institute of Molecular Plant Physiology; AlemaniaFil: Fernie, Alisdair R. Center of Plant Systems Biology and Biotechnology; BulgariaFil: Carrari, Fernando. Universidad de Buenos Aires. Facultad de Agronomía; ArgentinaFil: Carrari, Fernando. Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias; ArgentinaFil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaOxford University Press2024-01-11T14:38:58Z2024-01-11T14:38:58Z2021-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16535https://academic.oup.com/jxb/article/72/18/6490/62948591460-24310022-0957https://doi.org/10.1093/jxb/erab269Journal of Experimental Botany 72 (18) : 6490-6509 (Septiembre 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:10Zoai:localhost:20.500.12123/16535instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:11.825INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruits
title Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruits
spellingShingle Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruits
Dominguez, Pia Guadalupe
Botrytis cinerea
Metabolómica
Transcriptómica
Tomate
Estrés Abiótico
Maduramiento
Metabolomics
Transcriptomics
Tomatoes
ABA
Abiotic Stress
Ripening
title_short Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruits
title_full Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruits
title_fullStr Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruits
title_full_unstemmed Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruits
title_sort Multiomics analyses reveal the roles of the ASR1 transcription factor in tomato fruits
dc.creator.none.fl_str_mv Dominguez, Pia Guadalupe
Conti, Gabriela
Duffy, Tomas
Insani, Ester Marina
Alseekh, Saleh
Asurmendi, Sebastian
Fernie, Alisdair R.
Carrari, Fernando
author Dominguez, Pia Guadalupe
author_facet Dominguez, Pia Guadalupe
Conti, Gabriela
Duffy, Tomas
Insani, Ester Marina
Alseekh, Saleh
Asurmendi, Sebastian
Fernie, Alisdair R.
Carrari, Fernando
author_role author
author2 Conti, Gabriela
Duffy, Tomas
Insani, Ester Marina
Alseekh, Saleh
Asurmendi, Sebastian
Fernie, Alisdair R.
Carrari, Fernando
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Botrytis cinerea
Metabolómica
Transcriptómica
Tomate
Estrés Abiótico
Maduramiento
Metabolomics
Transcriptomics
Tomatoes
ABA
Abiotic Stress
Ripening
topic Botrytis cinerea
Metabolómica
Transcriptómica
Tomate
Estrés Abiótico
Maduramiento
Metabolomics
Transcriptomics
Tomatoes
ABA
Abiotic Stress
Ripening
dc.description.none.fl_txt_mv The transcription factor ASR1 (ABA, STRESS, RIPENING 1) plays multiple roles in plant responses to abiotic stresses as well as being involved in the regulation of central metabolism in several plant species. However, despite the high expression of ASR1 in tomato fruits, large scale analyses to uncover its function in fruits are still lacking. In order to study its function in the context of fruit ripening, we performed a multiomics analysis of ASR1-antisense transgenic tomato fruits at the transcriptome and metabolome levels. Our results indicate that ASR1 is involved in several pathways implicated in the fruit ripening process, including cell wall, amino acid, and carotenoid metabolism, as well as abiotic stress pathways. Moreover, we found that ASR1-antisense fruits are more susceptible to the infection by the necrotrophic fungus Botrytis cinerea. Given that ASR1 could be regulated by fruit ripening regulators such as FRUITFULL1/FRUITFULL2 (FUL1/FUL2), NON-RIPENING (NOR), and COLORLESS NON-RIPENING (CNR), we positioned it in the regulatory cascade of red ripe tomato fruits. These data extend the known range of functions of ASR1 as an important auxiliary regulator of tomato fruit ripening.
Instituto de Biotecnología
Fil: Dominguez, Pia Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Dominguez, Pia Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Conti, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conti, Gabriela. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Duffy, Tomás. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Duffy, Tomás. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Insani, Ester Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Insani, Ester Marina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Alseekh, Saleh. Max-Planck-Institute of Molecular Plant Physiology; Alemania
Fil: Alseekh, Saleh. Center of Plant Systems Biology and Biotechnology; Bulgaria
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernie, Alisdair R. Max-Planck-Institute of Molecular Plant Physiology; Alemania
Fil: Fernie, Alisdair R. Center of Plant Systems Biology and Biotechnology; Bulgaria
Fil: Carrari, Fernando. Universidad de Buenos Aires. Facultad de Agronomía; Argentina
Fil: Carrari, Fernando. Universidad de Buenos Aires. Instituto de Fisiología, Biología Molecular y Neurociencias; Argentina
Fil: Carrari, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The transcription factor ASR1 (ABA, STRESS, RIPENING 1) plays multiple roles in plant responses to abiotic stresses as well as being involved in the regulation of central metabolism in several plant species. However, despite the high expression of ASR1 in tomato fruits, large scale analyses to uncover its function in fruits are still lacking. In order to study its function in the context of fruit ripening, we performed a multiomics analysis of ASR1-antisense transgenic tomato fruits at the transcriptome and metabolome levels. Our results indicate that ASR1 is involved in several pathways implicated in the fruit ripening process, including cell wall, amino acid, and carotenoid metabolism, as well as abiotic stress pathways. Moreover, we found that ASR1-antisense fruits are more susceptible to the infection by the necrotrophic fungus Botrytis cinerea. Given that ASR1 could be regulated by fruit ripening regulators such as FRUITFULL1/FRUITFULL2 (FUL1/FUL2), NON-RIPENING (NOR), and COLORLESS NON-RIPENING (CNR), we positioned it in the regulatory cascade of red ripe tomato fruits. These data extend the known range of functions of ASR1 as an important auxiliary regulator of tomato fruit ripening.
publishDate 2021
dc.date.none.fl_str_mv 2021-09
2024-01-11T14:38:58Z
2024-01-11T14:38:58Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16535
https://academic.oup.com/jxb/article/72/18/6490/6294859
1460-2431
0022-0957
https://doi.org/10.1093/jxb/erab269
url http://hdl.handle.net/20.500.12123/16535
https://academic.oup.com/jxb/article/72/18/6490/6294859
https://doi.org/10.1093/jxb/erab269
identifier_str_mv 1460-2431
0022-0957
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv Journal of Experimental Botany 72 (18) : 6490-6509 (Septiembre 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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