Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean

Autores
Perez Brandan, Carolina; Arzeno, Jose Luis; Huidobro, Dina Jorgelina; Grumberg, Betiana Clarisa; Conforto, Erica Cinthia; Hilton, Sally; Bending, Gary D.; Meriles, Jose Manuel; Vargas Gil, Silvina
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems on biological, chemical and physical aspects of the soil, and to establish whether there was a correlation of these parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and crop yield. The tillage systems evaluated were direct seeding (DS), DS + scarifier (DS + S), minimum tillage (MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi (26.33 × 105 vs. 2.33 × 105 CFU g−1 dry soil), total bacteria (182 × 107 vs. 64 × 107 CFU g−1 dry soil), microbial respiration (0.77 mg CO2 g−1 week−1 vs. 0.45 mg CO2 g−1 week−1) and fluorescein diacetate (FDA) hydrolysis (4.17 ug fluorescein g−1 h−1 vs. 1.70 ug fluorescein g−1 h−1 in CT. Fungal and bacterial community fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, of Intergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillage system. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DS and CT was clearly different. DS samples contained significantly higher total microbial biomass than the other tillage treatments, but there were no significant differences in fungal biomass or any consistent trend with respect to stress index. Our results showed that microbial communities were more abundant and active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presented higher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were also improved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT, disease incidence in soybean was 54%. These differences were correlated to the higher microbial abundance and activity under DS, the biological component being a key factor determining soil capacity to suppress the soilborne pathogen.
EEA Salta
Fil: Perez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Arzeno, Jose Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Huidobro, Dina Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Grumberg, Betiana Clarisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Hilton, Sally. University of Warwick. School of Life Sciences; Reino Unido
Fil: Bending, Gary D. University of Warwick. School of Life Sciences; Reino Unido
Fil: Meriles, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Crop Protection 40 : 73-82 /October 2012)
Materia
Labranza
Propiedades Físico - Químicas Suelo
Soja
Macrophomina phaseolina
Biología del Suelo
Hongos del Suelo
Tillage
Soil Chemicophysical Properties
Soybeans
Soil Biology
Soil Fungi
Sistemas de Labranza
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4302

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oai_identifier_str oai:localhost:20.500.12123/4302
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spelling Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybeanPerez Brandan, CarolinaArzeno, Jose LuisHuidobro, Dina JorgelinaGrumberg, Betiana ClarisaConforto, Erica CinthiaHilton, SallyBending, Gary D.Meriles, Jose ManuelVargas Gil, SilvinaLabranzaPropiedades Físico - Químicas SueloSojaMacrophomina phaseolinaBiología del SueloHongos del SueloTillageSoil Chemicophysical PropertiesSoybeansSoil BiologySoil FungiSistemas de LabranzaA 20-year field experiment was employed with the aim of evaluating the effect of tillage systems on biological, chemical and physical aspects of the soil, and to establish whether there was a correlation of these parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and crop yield. The tillage systems evaluated were direct seeding (DS), DS + scarifier (DS + S), minimum tillage (MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi (26.33 × 105 vs. 2.33 × 105 CFU g−1 dry soil), total bacteria (182 × 107 vs. 64 × 107 CFU g−1 dry soil), microbial respiration (0.77 mg CO2 g−1 week−1 vs. 0.45 mg CO2 g−1 week−1) and fluorescein diacetate (FDA) hydrolysis (4.17 ug fluorescein g−1 h−1 vs. 1.70 ug fluorescein g−1 h−1 in CT. Fungal and bacterial community fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, of Intergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillage system. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DS and CT was clearly different. DS samples contained significantly higher total microbial biomass than the other tillage treatments, but there were no significant differences in fungal biomass or any consistent trend with respect to stress index. Our results showed that microbial communities were more abundant and active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presented higher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were also improved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT, disease incidence in soybean was 54%. These differences were correlated to the higher microbial abundance and activity under DS, the biological component being a key factor determining soil capacity to suppress the soilborne pathogen.EEA SaltaFil: Perez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Arzeno, Jose Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Huidobro, Dina Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; ArgentinaFil: Grumberg, Betiana Clarisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Hilton, Sally. University of Warwick. School of Life Sciences; Reino UnidoFil: Bending, Gary D. University of Warwick. School of Life Sciences; Reino UnidoFil: Meriles, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaElsevier2019-01-21T14:48:11Z2019-01-21T14:48:11Z2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0261219412001159http://hdl.handle.net/20.500.12123/43020261-2194https://doi.org/10.1016/j.cropro.2012.04.018Crop Protection 40 : 73-82 /October 2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:33Zoai:localhost:20.500.12123/4302instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:33.539INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
title Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
spellingShingle Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
Perez Brandan, Carolina
Labranza
Propiedades Físico - Químicas Suelo
Soja
Macrophomina phaseolina
Biología del Suelo
Hongos del Suelo
Tillage
Soil Chemicophysical Properties
Soybeans
Soil Biology
Soil Fungi
Sistemas de Labranza
title_short Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
title_full Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
title_fullStr Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
title_full_unstemmed Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
title_sort Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
dc.creator.none.fl_str_mv Perez Brandan, Carolina
Arzeno, Jose Luis
Huidobro, Dina Jorgelina
Grumberg, Betiana Clarisa
Conforto, Erica Cinthia
Hilton, Sally
Bending, Gary D.
Meriles, Jose Manuel
Vargas Gil, Silvina
author Perez Brandan, Carolina
author_facet Perez Brandan, Carolina
Arzeno, Jose Luis
Huidobro, Dina Jorgelina
Grumberg, Betiana Clarisa
Conforto, Erica Cinthia
Hilton, Sally
Bending, Gary D.
Meriles, Jose Manuel
Vargas Gil, Silvina
author_role author
author2 Arzeno, Jose Luis
Huidobro, Dina Jorgelina
Grumberg, Betiana Clarisa
Conforto, Erica Cinthia
Hilton, Sally
Bending, Gary D.
Meriles, Jose Manuel
Vargas Gil, Silvina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Labranza
Propiedades Físico - Químicas Suelo
Soja
Macrophomina phaseolina
Biología del Suelo
Hongos del Suelo
Tillage
Soil Chemicophysical Properties
Soybeans
Soil Biology
Soil Fungi
Sistemas de Labranza
topic Labranza
Propiedades Físico - Químicas Suelo
Soja
Macrophomina phaseolina
Biología del Suelo
Hongos del Suelo
Tillage
Soil Chemicophysical Properties
Soybeans
Soil Biology
Soil Fungi
Sistemas de Labranza
dc.description.none.fl_txt_mv A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems on biological, chemical and physical aspects of the soil, and to establish whether there was a correlation of these parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and crop yield. The tillage systems evaluated were direct seeding (DS), DS + scarifier (DS + S), minimum tillage (MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi (26.33 × 105 vs. 2.33 × 105 CFU g−1 dry soil), total bacteria (182 × 107 vs. 64 × 107 CFU g−1 dry soil), microbial respiration (0.77 mg CO2 g−1 week−1 vs. 0.45 mg CO2 g−1 week−1) and fluorescein diacetate (FDA) hydrolysis (4.17 ug fluorescein g−1 h−1 vs. 1.70 ug fluorescein g−1 h−1 in CT. Fungal and bacterial community fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, of Intergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillage system. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DS and CT was clearly different. DS samples contained significantly higher total microbial biomass than the other tillage treatments, but there were no significant differences in fungal biomass or any consistent trend with respect to stress index. Our results showed that microbial communities were more abundant and active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presented higher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were also improved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT, disease incidence in soybean was 54%. These differences were correlated to the higher microbial abundance and activity under DS, the biological component being a key factor determining soil capacity to suppress the soilborne pathogen.
EEA Salta
Fil: Perez Brandan, Carolina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Arzeno, Jose Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Huidobro, Dina Jorgelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina
Fil: Grumberg, Betiana Clarisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Hilton, Sally. University of Warwick. School of Life Sciences; Reino Unido
Fil: Bending, Gary D. University of Warwick. School of Life Sciences; Reino Unido
Fil: Meriles, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Vargas Gil, Silvina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems on biological, chemical and physical aspects of the soil, and to establish whether there was a correlation of these parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and crop yield. The tillage systems evaluated were direct seeding (DS), DS + scarifier (DS + S), minimum tillage (MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi (26.33 × 105 vs. 2.33 × 105 CFU g−1 dry soil), total bacteria (182 × 107 vs. 64 × 107 CFU g−1 dry soil), microbial respiration (0.77 mg CO2 g−1 week−1 vs. 0.45 mg CO2 g−1 week−1) and fluorescein diacetate (FDA) hydrolysis (4.17 ug fluorescein g−1 h−1 vs. 1.70 ug fluorescein g−1 h−1 in CT. Fungal and bacterial community fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, of Intergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillage system. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DS and CT was clearly different. DS samples contained significantly higher total microbial biomass than the other tillage treatments, but there were no significant differences in fungal biomass or any consistent trend with respect to stress index. Our results showed that microbial communities were more abundant and active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presented higher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were also improved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT, disease incidence in soybean was 54%. These differences were correlated to the higher microbial abundance and activity under DS, the biological component being a key factor determining soil capacity to suppress the soilborne pathogen.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
2019-01-21T14:48:11Z
2019-01-21T14:48:11Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0261219412001159
http://hdl.handle.net/20.500.12123/4302
0261-2194
https://doi.org/10.1016/j.cropro.2012.04.018
url https://www.sciencedirect.com/science/article/pii/S0261219412001159
http://hdl.handle.net/20.500.12123/4302
https://doi.org/10.1016/j.cropro.2012.04.018
identifier_str_mv 0261-2194
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Crop Protection 40 : 73-82 /October 2012)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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