Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid

Autores
Felipe, Verónica; Palma, Leopoldo; Yaryura, Pablo Marcelo
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The antifungal activity of Bacillus spp.strains has been already reported against a different number of phytopathogenicfungi3. Some species belonging to thisgenus (i.e. B. subtilis, B. pumilus and B. licheniformis) are considered GRAS (generally regarding as safe) microorganisms and exhibit potential to enhance plant growth by the production of an amplenumber of beneficial substances1,portraying these species as an environmentally friendly alternative tochemical fungicides. Macrophomina phaseolina (Tassi) Goid is a wide range soil-bornephytopathogenic fungus causing charcoal rot to several significant plant crops in Argentina (i.e. soybean, common bean,peanuts and corn)5,6 and is also capableto affect more than five hundred plant species worldwide4. Its ability to infect these hostplants is commonly correlated to the production of viable sclerotia, anon-sporic resistance structure constituted by a mass of densely packed hyphae, in the field5. In this work, we report theisolation of a Bacillus sp. strain 11.1 obtained from soil samples from Córdoba Province,Argentina2exhibiting a strongantagonistic activity against M. phaseolina. In order to analyse thepotential antagonistic effect of strain 11.1, both microorganisms were grown on dual culture platescontained potato dextrose agar (PDA) and tryptone soya agar (TSA) (1:1 v/v) and incubated for 48-72hours at 28ºC (Figure 1). An important and irreversible inhibition of the fungal growth was showed (Figure1A). In addition, the fungal mycelium located at the edge of the inhibited region was analysed bymicroscopic examination. The most evident antagonistic effect over the fungus was depicted by the lack ofblack pigmentation and the reduced size of formed sclerotiain comparison with the control (Figure 1B and C).
Fil: Felipe, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
Fil: Yaryura, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
Materia
BIOCONTROL
MACROPHOMINA PHASEOLINA
SCLEROTIA
MICROSCOPIC EXAMINATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/107499
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/107499
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) GoidActividad antagónica de una cepa de Bacillus sp. aislada en Córdoba, Argentina contra Macrophomina phaseolina (Tassi) GoidFelipe, VerónicaPalma, LeopoldoYaryura, Pablo MarceloBIOCONTROLMACROPHOMINA PHASEOLINASCLEROTIAMICROSCOPIC EXAMINATIONhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The antifungal activity of Bacillus spp.strains has been already reported against a different number of phytopathogenicfungi3. Some species belonging to thisgenus (i.e. B. subtilis, B. pumilus and B. licheniformis) are considered GRAS (generally regarding as safe) microorganisms and exhibit potential to enhance plant growth by the production of an amplenumber of beneficial substances1,portraying these species as an environmentally friendly alternative tochemical fungicides. Macrophomina phaseolina (Tassi) Goid is a wide range soil-bornephytopathogenic fungus causing charcoal rot to several significant plant crops in Argentina (i.e. soybean, common bean,peanuts and corn)5,6 and is also capableto affect more than five hundred plant species worldwide4. Its ability to infect these hostplants is commonly correlated to the production of viable sclerotia, anon-sporic resistance structure constituted by a mass of densely packed hyphae, in the field5. In this work, we report theisolation of a Bacillus sp. strain 11.1 obtained from soil samples from Córdoba Province,Argentina2exhibiting a strongantagonistic activity against M. phaseolina. In order to analyse thepotential antagonistic effect of strain 11.1, both microorganisms were grown on dual culture platescontained potato dextrose agar (PDA) and tryptone soya agar (TSA) (1:1 v/v) and incubated for 48-72hours at 28ºC (Figure 1). An important and irreversible inhibition of the fungal growth was showed (Figure1A). In addition, the fungal mycelium located at the edge of the inhibited region was analysed bymicroscopic examination. The most evident antagonistic effect over the fungus was depicted by the lack ofblack pigmentation and the reduced size of formed sclerotiain comparison with the control (Figure 1B and C).Fil: Felipe, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; ArgentinaFil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; ArgentinaFil: Yaryura, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; ArgentinaAsociación Argentina de Microbiología2017-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/107499Felipe, Verónica; Palma, Leopoldo; Yaryura, Pablo Marcelo; Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 49; 3-2017; 402-4030325-75411851-7617CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2016.08.008info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0325754116301171info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:09:30Zoai:ri.conicet.gov.ar:11336/107499instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:09:30.369CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid
Actividad antagónica de una cepa de Bacillus sp. aislada en Córdoba, Argentina contra Macrophomina phaseolina (Tassi) Goid
title Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid
spellingShingle Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid
Felipe, Verónica
BIOCONTROL
MACROPHOMINA PHASEOLINA
SCLEROTIA
MICROSCOPIC EXAMINATION
title_short Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid
title_full Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid
title_fullStr Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid
title_full_unstemmed Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid
title_sort Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid
dc.creator.none.fl_str_mv Felipe, Verónica
Palma, Leopoldo
Yaryura, Pablo Marcelo
author Felipe, Verónica
author_facet Felipe, Verónica
Palma, Leopoldo
Yaryura, Pablo Marcelo
author_role author
author2 Palma, Leopoldo
Yaryura, Pablo Marcelo
author2_role author
author
dc.subject.none.fl_str_mv BIOCONTROL
MACROPHOMINA PHASEOLINA
SCLEROTIA
MICROSCOPIC EXAMINATION
topic BIOCONTROL
MACROPHOMINA PHASEOLINA
SCLEROTIA
MICROSCOPIC EXAMINATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The antifungal activity of Bacillus spp.strains has been already reported against a different number of phytopathogenicfungi3. Some species belonging to thisgenus (i.e. B. subtilis, B. pumilus and B. licheniformis) are considered GRAS (generally regarding as safe) microorganisms and exhibit potential to enhance plant growth by the production of an amplenumber of beneficial substances1,portraying these species as an environmentally friendly alternative tochemical fungicides. Macrophomina phaseolina (Tassi) Goid is a wide range soil-bornephytopathogenic fungus causing charcoal rot to several significant plant crops in Argentina (i.e. soybean, common bean,peanuts and corn)5,6 and is also capableto affect more than five hundred plant species worldwide4. Its ability to infect these hostplants is commonly correlated to the production of viable sclerotia, anon-sporic resistance structure constituted by a mass of densely packed hyphae, in the field5. In this work, we report theisolation of a Bacillus sp. strain 11.1 obtained from soil samples from Córdoba Province,Argentina2exhibiting a strongantagonistic activity against M. phaseolina. In order to analyse thepotential antagonistic effect of strain 11.1, both microorganisms were grown on dual culture platescontained potato dextrose agar (PDA) and tryptone soya agar (TSA) (1:1 v/v) and incubated for 48-72hours at 28ºC (Figure 1). An important and irreversible inhibition of the fungal growth was showed (Figure1A). In addition, the fungal mycelium located at the edge of the inhibited region was analysed bymicroscopic examination. The most evident antagonistic effect over the fungus was depicted by the lack ofblack pigmentation and the reduced size of formed sclerotiain comparison with the control (Figure 1B and C).
Fil: Felipe, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
Fil: Palma, Leopoldo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
Fil: Yaryura, Pablo Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Centro de Investigaciones y Transferencia de Villa María. Universidad Nacional de Villa María. Centro de Investigaciones y Transferencia de Villa María; Argentina
description The antifungal activity of Bacillus spp.strains has been already reported against a different number of phytopathogenicfungi3. Some species belonging to thisgenus (i.e. B. subtilis, B. pumilus and B. licheniformis) are considered GRAS (generally regarding as safe) microorganisms and exhibit potential to enhance plant growth by the production of an amplenumber of beneficial substances1,portraying these species as an environmentally friendly alternative tochemical fungicides. Macrophomina phaseolina (Tassi) Goid is a wide range soil-bornephytopathogenic fungus causing charcoal rot to several significant plant crops in Argentina (i.e. soybean, common bean,peanuts and corn)5,6 and is also capableto affect more than five hundred plant species worldwide4. Its ability to infect these hostplants is commonly correlated to the production of viable sclerotia, anon-sporic resistance structure constituted by a mass of densely packed hyphae, in the field5. In this work, we report theisolation of a Bacillus sp. strain 11.1 obtained from soil samples from Córdoba Province,Argentina2exhibiting a strongantagonistic activity against M. phaseolina. In order to analyse thepotential antagonistic effect of strain 11.1, both microorganisms were grown on dual culture platescontained potato dextrose agar (PDA) and tryptone soya agar (TSA) (1:1 v/v) and incubated for 48-72hours at 28ºC (Figure 1). An important and irreversible inhibition of the fungal growth was showed (Figure1A). In addition, the fungal mycelium located at the edge of the inhibited region was analysed bymicroscopic examination. The most evident antagonistic effect over the fungus was depicted by the lack ofblack pigmentation and the reduced size of formed sclerotiain comparison with the control (Figure 1B and C).
publishDate 2017
dc.date.none.fl_str_mv 2017-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/107499
Felipe, Verónica; Palma, Leopoldo; Yaryura, Pablo Marcelo; Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 49; 3-2017; 402-403
0325-7541
1851-7617
CONICET Digital
CONICET
url http://hdl.handle.net/11336/107499
identifier_str_mv Felipe, Verónica; Palma, Leopoldo; Yaryura, Pablo Marcelo; Antagonistic activity of Bacillus sp. strain isolated in Córdoba, Argentina against Macrophomina phaseolina (Tassi) Goid; Asociación Argentina de Microbiología; Revista Argentina de Microbiología; 49; 3-2017; 402-403
0325-7541
1851-7617
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.ram.2016.08.008
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0325754116301171
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Asociación Argentina de Microbiología
publisher.none.fl_str_mv Asociación Argentina de Microbiología
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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