Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean

Autores
Perez Brandan, Carolina Gabriela; Arzeno, José Luis; Huidobro, Jorgelina; Grümberg, Betiana Clarisa; Conforto, Erica Cinthia; Hilton, Sally; Bending, Gary D.; Meriles, Jose Manuel; Vargas Gil, Silvina
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems onbiological, chemical and physical aspects of the soil, and to establish whether there was a correlation ofthese parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and cropyield. The tillage systems evaluated were direct seeding (DS), DS þ scarifier (DS þ S), minimum tillage(MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi(26.33 105 vs. 2.33 105 CFU g1 dry soil), total bacteria (182 107 vs. 64 107 CFU g1 dry soil),microbial respiration (0.77 mg CO2 g1 week1 vs. 0.45 mg CO2 g1 week1) and fluorescein diacetate(FDA) hydrolysis (4.17 ug fluorescein g1 h1 vs. 1.70 ug fluorescein g1 h1 in CT. Fungal and bacterialcommunity fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, ofIntergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillagesystem. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DSand CT was clearly different. DS samples contained significantly higher total microbial biomass than theother tillage treatments, but there were no significant differences in fungal biomass or any consistenttrend with respect to stress index. Our results showed that microbial communities were more abundantand active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presentedhigher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were alsoimproved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT,disease incidence in soybean was 54%. These differences were correlated to the higher microbialabundance and activity under DS, the biological component being a key factor determining soil capacityto suppress the soilborne pathogen.
Fil: Perez Brandan, Carolina Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arzeno, José Luis. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Huidobro, Jorgelina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Grümberg, Betiana Clarisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Hilton, Sally. University of Warwick; Reino Unido
Fil: Bending, Gary D.. University of Warwick; Reino Unido
Fil: Meriles, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Materia
Soybean
Tillage systems
Microbial communities
T-RFLP
FAME
Soil quality
Soilborne fungi
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/271145
Acceder
id CONICETDig_d6d1b308d1a55f2290718af99ce77bb1
oai_identifier_str oai:ri.conicet.gov.ar:11336/271145
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybeanPerez Brandan, Carolina GabrielaArzeno, José LuisHuidobro, JorgelinaGrümberg, Betiana ClarisaConforto, Erica CinthiaHilton, SallyBending, Gary D.Meriles, Jose ManuelVargas Gil, SilvinaSoybeanTillage systemsMicrobial communitiesT-RFLPFAMESoil qualitySoilborne fungihttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems onbiological, chemical and physical aspects of the soil, and to establish whether there was a correlation ofthese parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and cropyield. The tillage systems evaluated were direct seeding (DS), DS þ scarifier (DS þ S), minimum tillage(MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi(26.33 105 vs. 2.33 105 CFU g1 dry soil), total bacteria (182 107 vs. 64 107 CFU g1 dry soil),microbial respiration (0.77 mg CO2 g1 week1 vs. 0.45 mg CO2 g1 week1) and fluorescein diacetate(FDA) hydrolysis (4.17 ug fluorescein g1 h1 vs. 1.70 ug fluorescein g1 h1 in CT. Fungal and bacterialcommunity fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, ofIntergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillagesystem. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DSand CT was clearly different. DS samples contained significantly higher total microbial biomass than theother tillage treatments, but there were no significant differences in fungal biomass or any consistenttrend with respect to stress index. Our results showed that microbial communities were more abundantand active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presentedhigher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were alsoimproved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT,disease incidence in soybean was 54%. These differences were correlated to the higher microbialabundance and activity under DS, the biological component being a key factor determining soil capacityto suppress the soilborne pathogen.Fil: Perez Brandan, Carolina Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arzeno, José Luis. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Huidobro, Jorgelina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Grümberg, Betiana Clarisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Hilton, Sally. University of Warwick; Reino UnidoFil: Bending, Gary D.. University of Warwick; Reino UnidoFil: Meriles, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaElsevier2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271145Perez Brandan, Carolina Gabriela; Arzeno, José Luis; Huidobro, Jorgelina; Grümberg, Betiana Clarisa; Conforto, Erica Cinthia; et al.; Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean; Elsevier; Crop Protection; 40; 10-2012; 73-820261-2194CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cropro.2012.04.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:07:49Zoai:ri.conicet.gov.ar:11336/271145instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:07:49.464CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
title Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
spellingShingle Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
Perez Brandan, Carolina Gabriela
Soybean
Tillage systems
Microbial communities
T-RFLP
FAME
Soil quality
Soilborne fungi
title_short Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
title_full Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
title_fullStr Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
title_full_unstemmed Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
title_sort Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
dc.creator.none.fl_str_mv Perez Brandan, Carolina Gabriela
Arzeno, José Luis
Huidobro, Jorgelina
Grümberg, Betiana Clarisa
Conforto, Erica Cinthia
Hilton, Sally
Bending, Gary D.
Meriles, Jose Manuel
Vargas Gil, Silvina
author Perez Brandan, Carolina Gabriela
author_facet Perez Brandan, Carolina Gabriela
Arzeno, José Luis
Huidobro, Jorgelina
Grümberg, Betiana Clarisa
Conforto, Erica Cinthia
Hilton, Sally
Bending, Gary D.
Meriles, Jose Manuel
Vargas Gil, Silvina
author_role author
author2 Arzeno, José Luis
Huidobro, Jorgelina
Grümberg, Betiana Clarisa
Conforto, Erica Cinthia
Hilton, Sally
Bending, Gary D.
Meriles, Jose Manuel
Vargas Gil, Silvina
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Soybean
Tillage systems
Microbial communities
T-RFLP
FAME
Soil quality
Soilborne fungi
topic Soybean
Tillage systems
Microbial communities
T-RFLP
FAME
Soil quality
Soilborne fungi
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.1
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems onbiological, chemical and physical aspects of the soil, and to establish whether there was a correlation ofthese parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and cropyield. The tillage systems evaluated were direct seeding (DS), DS þ scarifier (DS þ S), minimum tillage(MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi(26.33 105 vs. 2.33 105 CFU g1 dry soil), total bacteria (182 107 vs. 64 107 CFU g1 dry soil),microbial respiration (0.77 mg CO2 g1 week1 vs. 0.45 mg CO2 g1 week1) and fluorescein diacetate(FDA) hydrolysis (4.17 ug fluorescein g1 h1 vs. 1.70 ug fluorescein g1 h1 in CT. Fungal and bacterialcommunity fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, ofIntergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillagesystem. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DSand CT was clearly different. DS samples contained significantly higher total microbial biomass than theother tillage treatments, but there were no significant differences in fungal biomass or any consistenttrend with respect to stress index. Our results showed that microbial communities were more abundantand active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presentedhigher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were alsoimproved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT,disease incidence in soybean was 54%. These differences were correlated to the higher microbialabundance and activity under DS, the biological component being a key factor determining soil capacityto suppress the soilborne pathogen.
Fil: Perez Brandan, Carolina Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arzeno, José Luis. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Huidobro, Jorgelina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Grümberg, Betiana Clarisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Hilton, Sally. University of Warwick; Reino Unido
Fil: Bending, Gary D.. University of Warwick; Reino Unido
Fil: Meriles, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
description A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems onbiological, chemical and physical aspects of the soil, and to establish whether there was a correlation ofthese parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and cropyield. The tillage systems evaluated were direct seeding (DS), DS þ scarifier (DS þ S), minimum tillage(MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi(26.33 105 vs. 2.33 105 CFU g1 dry soil), total bacteria (182 107 vs. 64 107 CFU g1 dry soil),microbial respiration (0.77 mg CO2 g1 week1 vs. 0.45 mg CO2 g1 week1) and fluorescein diacetate(FDA) hydrolysis (4.17 ug fluorescein g1 h1 vs. 1.70 ug fluorescein g1 h1 in CT. Fungal and bacterialcommunity fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, ofIntergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillagesystem. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DSand CT was clearly different. DS samples contained significantly higher total microbial biomass than theother tillage treatments, but there were no significant differences in fungal biomass or any consistenttrend with respect to stress index. Our results showed that microbial communities were more abundantand active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presentedhigher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were alsoimproved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT,disease incidence in soybean was 54%. These differences were correlated to the higher microbialabundance and activity under DS, the biological component being a key factor determining soil capacityto suppress the soilborne pathogen.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/271145
Perez Brandan, Carolina Gabriela; Arzeno, José Luis; Huidobro, Jorgelina; Grümberg, Betiana Clarisa; Conforto, Erica Cinthia; et al.; Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean; Elsevier; Crop Protection; 40; 10-2012; 73-82
0261-2194
CONICET Digital
CONICET
url http://hdl.handle.net/11336/271145
identifier_str_mv Perez Brandan, Carolina Gabriela; Arzeno, José Luis; Huidobro, Jorgelina; Grümberg, Betiana Clarisa; Conforto, Erica Cinthia; et al.; Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean; Elsevier; Crop Protection; 40; 10-2012; 73-82
0261-2194
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cropro.2012.04.018
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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