Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean
- Autores
- Perez Brandan, Carolina Gabriela; Arzeno, José Luis; Huidobro, Jorgelina; Grümberg, Betiana Clarisa; Conforto, Erica Cinthia; Hilton, Sally; Bending, Gary D.; Meriles, Jose Manuel; Vargas Gil, Silvina
- Año de publicación
- 2012
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems onbiological, chemical and physical aspects of the soil, and to establish whether there was a correlation ofthese parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and cropyield. The tillage systems evaluated were direct seeding (DS), DS þ scarifier (DS þ S), minimum tillage(MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi(26.33 105 vs. 2.33 105 CFU g1 dry soil), total bacteria (182 107 vs. 64 107 CFU g1 dry soil),microbial respiration (0.77 mg CO2 g1 week1 vs. 0.45 mg CO2 g1 week1) and fluorescein diacetate(FDA) hydrolysis (4.17 ug fluorescein g1 h1 vs. 1.70 ug fluorescein g1 h1 in CT. Fungal and bacterialcommunity fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, ofIntergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillagesystem. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DSand CT was clearly different. DS samples contained significantly higher total microbial biomass than theother tillage treatments, but there were no significant differences in fungal biomass or any consistenttrend with respect to stress index. Our results showed that microbial communities were more abundantand active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presentedhigher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were alsoimproved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT,disease incidence in soybean was 54%. These differences were correlated to the higher microbialabundance and activity under DS, the biological component being a key factor determining soil capacityto suppress the soilborne pathogen.
Fil: Perez Brandan, Carolina Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Arzeno, José Luis. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Huidobro, Jorgelina. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Grümberg, Betiana Clarisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina
Fil: Hilton, Sally. University of Warwick; Reino Unido
Fil: Bending, Gary D.. University of Warwick; Reino Unido
Fil: Meriles, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
Fil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina - Materia
-
Soybean
Tillage systems
Microbial communities
T-RFLP
FAME
Soil quality
Soilborne fungi - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/271145
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Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybeanPerez Brandan, Carolina GabrielaArzeno, José LuisHuidobro, JorgelinaGrümberg, Betiana ClarisaConforto, Erica CinthiaHilton, SallyBending, Gary D.Meriles, Jose ManuelVargas Gil, SilvinaSoybeanTillage systemsMicrobial communitiesT-RFLPFAMESoil qualitySoilborne fungihttps://purl.org/becyt/ford/4.1https://purl.org/becyt/ford/4A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems onbiological, chemical and physical aspects of the soil, and to establish whether there was a correlation ofthese parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and cropyield. The tillage systems evaluated were direct seeding (DS), DS þ scarifier (DS þ S), minimum tillage(MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi(26.33 105 vs. 2.33 105 CFU g1 dry soil), total bacteria (182 107 vs. 64 107 CFU g1 dry soil),microbial respiration (0.77 mg CO2 g1 week1 vs. 0.45 mg CO2 g1 week1) and fluorescein diacetate(FDA) hydrolysis (4.17 ug fluorescein g1 h1 vs. 1.70 ug fluorescein g1 h1 in CT. Fungal and bacterialcommunity fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, ofIntergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillagesystem. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DSand CT was clearly different. DS samples contained significantly higher total microbial biomass than theother tillage treatments, but there were no significant differences in fungal biomass or any consistenttrend with respect to stress index. Our results showed that microbial communities were more abundantand active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presentedhigher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were alsoimproved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT,disease incidence in soybean was 54%. These differences were correlated to the higher microbialabundance and activity under DS, the biological component being a key factor determining soil capacityto suppress the soilborne pathogen.Fil: Perez Brandan, Carolina Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Arzeno, José Luis. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Huidobro, Jorgelina. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Grümberg, Betiana Clarisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaFil: Hilton, Sally. University of Warwick; Reino UnidoFil: Bending, Gary D.. University of Warwick; Reino UnidoFil: Meriles, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; ArgentinaElsevier2012-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/271145Perez Brandan, Carolina Gabriela; Arzeno, José Luis; Huidobro, Jorgelina; Grümberg, Betiana Clarisa; Conforto, Erica Cinthia; et al.; Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean; Elsevier; Crop Protection; 40; 10-2012; 73-820261-2194CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cropro.2012.04.018info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:35:27Zoai:ri.conicet.gov.ar:11336/271145instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:35:27.295CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
title |
Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
spellingShingle |
Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean Perez Brandan, Carolina Gabriela Soybean Tillage systems Microbial communities T-RFLP FAME Soil quality Soilborne fungi |
title_short |
Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
title_full |
Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
title_fullStr |
Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
title_full_unstemmed |
Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
title_sort |
Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean |
dc.creator.none.fl_str_mv |
Perez Brandan, Carolina Gabriela Arzeno, José Luis Huidobro, Jorgelina Grümberg, Betiana Clarisa Conforto, Erica Cinthia Hilton, Sally Bending, Gary D. Meriles, Jose Manuel Vargas Gil, Silvina |
author |
Perez Brandan, Carolina Gabriela |
author_facet |
Perez Brandan, Carolina Gabriela Arzeno, José Luis Huidobro, Jorgelina Grümberg, Betiana Clarisa Conforto, Erica Cinthia Hilton, Sally Bending, Gary D. Meriles, Jose Manuel Vargas Gil, Silvina |
author_role |
author |
author2 |
Arzeno, José Luis Huidobro, Jorgelina Grümberg, Betiana Clarisa Conforto, Erica Cinthia Hilton, Sally Bending, Gary D. Meriles, Jose Manuel Vargas Gil, Silvina |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Soybean Tillage systems Microbial communities T-RFLP FAME Soil quality Soilborne fungi |
topic |
Soybean Tillage systems Microbial communities T-RFLP FAME Soil quality Soilborne fungi |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.1 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems onbiological, chemical and physical aspects of the soil, and to establish whether there was a correlation ofthese parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and cropyield. The tillage systems evaluated were direct seeding (DS), DS þ scarifier (DS þ S), minimum tillage(MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi(26.33 105 vs. 2.33 105 CFU g1 dry soil), total bacteria (182 107 vs. 64 107 CFU g1 dry soil),microbial respiration (0.77 mg CO2 g1 week1 vs. 0.45 mg CO2 g1 week1) and fluorescein diacetate(FDA) hydrolysis (4.17 ug fluorescein g1 h1 vs. 1.70 ug fluorescein g1 h1 in CT. Fungal and bacterialcommunity fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, ofIntergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillagesystem. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DSand CT was clearly different. DS samples contained significantly higher total microbial biomass than theother tillage treatments, but there were no significant differences in fungal biomass or any consistenttrend with respect to stress index. Our results showed that microbial communities were more abundantand active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presentedhigher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were alsoimproved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT,disease incidence in soybean was 54%. These differences were correlated to the higher microbialabundance and activity under DS, the biological component being a key factor determining soil capacityto suppress the soilborne pathogen. Fil: Perez Brandan, Carolina Gabriela. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Salta-Jujuy. Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Arzeno, José Luis. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Huidobro, Jorgelina. Instituto Nacional de Tecnología Agropecuaria; Argentina Fil: Grümberg, Betiana Clarisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina Fil: Conforto, Erica Cinthia. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina Fil: Hilton, Sally. University of Warwick; Reino Unido Fil: Bending, Gary D.. University of Warwick; Reino Unido Fil: Meriles, Jose Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina Fil: Vargas Gil, Silvina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina |
description |
A 20-year field experiment was employed with the aim of evaluating the effect of tillage systems onbiological, chemical and physical aspects of the soil, and to establish whether there was a correlation ofthese parameters with the incidence of charcoal rot (Macrophomina phaseolina) of soybean and cropyield. The tillage systems evaluated were direct seeding (DS), DS þ scarifier (DS þ S), minimum tillage(MT) and conventional tillage (CT). DS presented higher values than CT in culturable total fungi(26.33 105 vs. 2.33 105 CFU g1 dry soil), total bacteria (182 107 vs. 64 107 CFU g1 dry soil),microbial respiration (0.77 mg CO2 g1 week1 vs. 0.45 mg CO2 g1 week1) and fluorescein diacetate(FDA) hydrolysis (4.17 ug fluorescein g1 h1 vs. 1.70 ug fluorescein g1 h1 in CT. Fungal and bacterialcommunity fingerprints, by terminal restriction fragment length polymorphism (T-RFLP) analysis, ofIntergenic spacer regions of rRNA and 16S rRNA genes, respectively, were influenced by the tillagesystem. Also FAME (fatty acid methyl ester) profiles showed that microbial community structure in DSand CT was clearly different. DS samples contained significantly higher total microbial biomass than theother tillage treatments, but there were no significant differences in fungal biomass or any consistenttrend with respect to stress index. Our results showed that microbial communities were more abundantand active in DS than in CT in response to high nutrient content in soil. Indeed, DS systems presentedhigher soil OM, total N, K and Ca than CT. Electrical conductivity and aggregate stability (AS) were alsoimproved by DS. Soybean grown in high-quality soil was not affected by charcoal rot, however, under CT,disease incidence in soybean was 54%. These differences were correlated to the higher microbialabundance and activity under DS, the biological component being a key factor determining soil capacityto suppress the soilborne pathogen. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-10 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/271145 Perez Brandan, Carolina Gabriela; Arzeno, José Luis; Huidobro, Jorgelina; Grümberg, Betiana Clarisa; Conforto, Erica Cinthia; et al.; Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean; Elsevier; Crop Protection; 40; 10-2012; 73-82 0261-2194 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/271145 |
identifier_str_mv |
Perez Brandan, Carolina Gabriela; Arzeno, José Luis; Huidobro, Jorgelina; Grümberg, Betiana Clarisa; Conforto, Erica Cinthia; et al.; Long-term effect of tillage systems on soil microbiological, chemical and physical parameters and the incidence of charcoal rot by Macrophomina phaseolina (Tassi) Goid in soybean; Elsevier; Crop Protection; 40; 10-2012; 73-82 0261-2194 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cropro.2012.04.018 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
reponame_str |
CONICET Digital (CONICET) |
collection |
CONICET Digital (CONICET) |
instname_str |
Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.name.fl_str_mv |
CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614372918296576 |
score |
13.070432 |