The role of melanin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis

Autores
Rangel Montoya, Edelweiss Airam; Paolinelli, Marcos; Rolshausen, Philippe; Hernandez Martinez, Rufina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lasiodiplodia (Botryosphaeriaceae) includes fungi that are considered among the most aggressive to grapevine, capable of causing cankers and necrotic lesions which eventually lead to death of host plants. A common characteristic of this genus is the presence of melanin in conidia and mycelium. Melanin is produced by the oxidation of phenolic and/or indolic compounds. For some fungi, this pigment is an essential factor for pathogenicity. This study characterized the types and the roles of melanin produced by Lasiodiplodia gilanensis. Using specific melanin inhibitors, L. gilanensis was shown to synthesize DOPA-melanin, DHN-melanin, and pyomelanin. DOPA-melanin was shown to be involved in production of aerial mycelium and protection against enzymatic lysis and oxidative stress; DHN-melanin to be involved in ramification of mycelium when exposed to nutrient deficiency; and pyomelanin to be related with hyphae development. The fungus used tyrosine as a precursor of DOPAmelanin and as carbon and nitrogen sources, and produced melanin inside the piths of infected plants. Genes involved in melanin synthesis were conserved among the Botryosphaeriaceae, highlighting the importance of melanin in this family.
EEA Mendoza
Fil: Rangel Montoya, Edelweiss Airam. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). Departamento de Microbiología; México
Fil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Paolinelli, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; Argentina
Fil: Rolshausen, Philippe. University of California Riverside. Department of Botany and Plant Sciences; Estados Unidos
Fil: Hernandez Martinez, Rufina. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). Departamento de Microbiología; México
Fuente
Phytopathologia mediterranea 59 (3) : 549-563. (2020)
Materia
Melanins
Grapevines
Plant Diseases
Fungal Diseases
Tyrosine
Catabolism
Trunks
Melaninas
Vid
Enfermedades de las Plantas
Botryosphaeria
Enfermedades Fungosas
Tirosina
Catabolismo
Tronco
Lasiodiplodia Gilanensis
Botryosphaeria Dieback
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9129
Acceder
id INTADig_6b0ca22e7a8079e311f57e2e718e3702
oai_identifier_str oai:localhost:20.500.12123/9129
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling The role of melanin in the grapevine trunk disease pathogen Lasiodiplodia gilanensisRangel Montoya, Edelweiss AiramPaolinelli, MarcosRolshausen, PhilippeHernandez Martinez, RufinaMelaninsGrapevinesPlant DiseasesFungal DiseasesTyrosineCatabolismTrunksMelaninasVidEnfermedades de las PlantasBotryosphaeriaEnfermedades FungosasTirosinaCatabolismoTroncoLasiodiplodia GilanensisBotryosphaeria DiebackLasiodiplodia (Botryosphaeriaceae) includes fungi that are considered among the most aggressive to grapevine, capable of causing cankers and necrotic lesions which eventually lead to death of host plants. A common characteristic of this genus is the presence of melanin in conidia and mycelium. Melanin is produced by the oxidation of phenolic and/or indolic compounds. For some fungi, this pigment is an essential factor for pathogenicity. This study characterized the types and the roles of melanin produced by Lasiodiplodia gilanensis. Using specific melanin inhibitors, L. gilanensis was shown to synthesize DOPA-melanin, DHN-melanin, and pyomelanin. DOPA-melanin was shown to be involved in production of aerial mycelium and protection against enzymatic lysis and oxidative stress; DHN-melanin to be involved in ramification of mycelium when exposed to nutrient deficiency; and pyomelanin to be related with hyphae development. The fungus used tyrosine as a precursor of DOPAmelanin and as carbon and nitrogen sources, and produced melanin inside the piths of infected plants. Genes involved in melanin synthesis were conserved among the Botryosphaeriaceae, highlighting the importance of melanin in this family.EEA MendozaFil: Rangel Montoya, Edelweiss Airam. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). Departamento de Microbiología; MéxicoFil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; ArgentinaFil: Paolinelli, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; ArgentinaFil: Rolshausen, Philippe. University of California Riverside. Department of Botany and Plant Sciences; Estados UnidosFil: Hernandez Martinez, Rufina. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). Departamento de Microbiología; MéxicoFirenze University PressMediterranean Phytopathological Union2021-04-19T18:36:49Z2021-04-19T18:36:49Z2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9129https://oajournals.fupress.net/index.php/pm/article/view/11685E. A. Rangel-Montoya, M. Paolinelli, P. Rolshausen, R. Hernan-dez-Martinez (2020) The role of mela-nin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis. Phytopathologia Mediterranea 59(3): 549-563. doi: 10.14601/Phyto-116850031-94651593-2095 (Online)https://doi.org/10.14601/Phyto-11685Phytopathologia mediterranea 59 (3) : 549-563. (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:11Zoai:localhost:20.500.12123/9129instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:11.827INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv The role of melanin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis
title The role of melanin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis
spellingShingle The role of melanin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis
Rangel Montoya, Edelweiss Airam
Melanins
Grapevines
Plant Diseases
Fungal Diseases
Tyrosine
Catabolism
Trunks
Melaninas
Vid
Enfermedades de las Plantas
Botryosphaeria
Enfermedades Fungosas
Tirosina
Catabolismo
Tronco
Lasiodiplodia Gilanensis
Botryosphaeria Dieback
title_short The role of melanin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis
title_full The role of melanin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis
title_fullStr The role of melanin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis
title_full_unstemmed The role of melanin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis
title_sort The role of melanin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis
dc.creator.none.fl_str_mv Rangel Montoya, Edelweiss Airam
Paolinelli, Marcos
Rolshausen, Philippe
Hernandez Martinez, Rufina
author Rangel Montoya, Edelweiss Airam
author_facet Rangel Montoya, Edelweiss Airam
Paolinelli, Marcos
Rolshausen, Philippe
Hernandez Martinez, Rufina
author_role author
author2 Paolinelli, Marcos
Rolshausen, Philippe
Hernandez Martinez, Rufina
author2_role author
author
author
dc.contributor.none.fl_str_mv Mediterranean Phytopathological Union
dc.subject.none.fl_str_mv Melanins
Grapevines
Plant Diseases
Fungal Diseases
Tyrosine
Catabolism
Trunks
Melaninas
Vid
Enfermedades de las Plantas
Botryosphaeria
Enfermedades Fungosas
Tirosina
Catabolismo
Tronco
Lasiodiplodia Gilanensis
Botryosphaeria Dieback
topic Melanins
Grapevines
Plant Diseases
Fungal Diseases
Tyrosine
Catabolism
Trunks
Melaninas
Vid
Enfermedades de las Plantas
Botryosphaeria
Enfermedades Fungosas
Tirosina
Catabolismo
Tronco
Lasiodiplodia Gilanensis
Botryosphaeria Dieback
dc.description.none.fl_txt_mv Lasiodiplodia (Botryosphaeriaceae) includes fungi that are considered among the most aggressive to grapevine, capable of causing cankers and necrotic lesions which eventually lead to death of host plants. A common characteristic of this genus is the presence of melanin in conidia and mycelium. Melanin is produced by the oxidation of phenolic and/or indolic compounds. For some fungi, this pigment is an essential factor for pathogenicity. This study characterized the types and the roles of melanin produced by Lasiodiplodia gilanensis. Using specific melanin inhibitors, L. gilanensis was shown to synthesize DOPA-melanin, DHN-melanin, and pyomelanin. DOPA-melanin was shown to be involved in production of aerial mycelium and protection against enzymatic lysis and oxidative stress; DHN-melanin to be involved in ramification of mycelium when exposed to nutrient deficiency; and pyomelanin to be related with hyphae development. The fungus used tyrosine as a precursor of DOPAmelanin and as carbon and nitrogen sources, and produced melanin inside the piths of infected plants. Genes involved in melanin synthesis were conserved among the Botryosphaeriaceae, highlighting the importance of melanin in this family.
EEA Mendoza
Fil: Rangel Montoya, Edelweiss Airam. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). Departamento de Microbiología; México
Fil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mendoza; Argentina
Fil: Paolinelli, Marcos. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina; Argentina
Fil: Rolshausen, Philippe. University of California Riverside. Department of Botany and Plant Sciences; Estados Unidos
Fil: Hernandez Martinez, Rufina. Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE). Departamento de Microbiología; México
description Lasiodiplodia (Botryosphaeriaceae) includes fungi that are considered among the most aggressive to grapevine, capable of causing cankers and necrotic lesions which eventually lead to death of host plants. A common characteristic of this genus is the presence of melanin in conidia and mycelium. Melanin is produced by the oxidation of phenolic and/or indolic compounds. For some fungi, this pigment is an essential factor for pathogenicity. This study characterized the types and the roles of melanin produced by Lasiodiplodia gilanensis. Using specific melanin inhibitors, L. gilanensis was shown to synthesize DOPA-melanin, DHN-melanin, and pyomelanin. DOPA-melanin was shown to be involved in production of aerial mycelium and protection against enzymatic lysis and oxidative stress; DHN-melanin to be involved in ramification of mycelium when exposed to nutrient deficiency; and pyomelanin to be related with hyphae development. The fungus used tyrosine as a precursor of DOPAmelanin and as carbon and nitrogen sources, and produced melanin inside the piths of infected plants. Genes involved in melanin synthesis were conserved among the Botryosphaeriaceae, highlighting the importance of melanin in this family.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
2021-04-19T18:36:49Z
2021-04-19T18:36:49Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9129
https://oajournals.fupress.net/index.php/pm/article/view/11685
E. A. Rangel-Montoya, M. Paolinelli, P. Rolshausen, R. Hernan-dez-Martinez (2020) The role of mela-nin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis. Phytopathologia Mediterranea 59(3): 549-563. doi: 10.14601/Phyto-11685
0031-9465
1593-2095 (Online)
https://doi.org/10.14601/Phyto-11685
url http://hdl.handle.net/20.500.12123/9129
https://oajournals.fupress.net/index.php/pm/article/view/11685
https://doi.org/10.14601/Phyto-11685
identifier_str_mv E. A. Rangel-Montoya, M. Paolinelli, P. Rolshausen, R. Hernan-dez-Martinez (2020) The role of mela-nin in the grapevine trunk disease pathogen Lasiodiplodia gilanensis. Phytopathologia Mediterranea 59(3): 549-563. doi: 10.14601/Phyto-11685
0031-9465
1593-2095 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Firenze University Press
publisher.none.fl_str_mv Firenze University Press
dc.source.none.fl_str_mv Phytopathologia mediterranea 59 (3) : 549-563. (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619152752377856
score 12.559606

Publicaciones similares