Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae

Autores
Rangel Montoya Edelweiss; Paolinelli, Marcos; Hernández Martínez Rufina
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Lasiodiplodia spp. cause degenerative diseases, dieback and plant death in a wide range of woody plants. To understand the fungal behavior of the genus we are using Lasiodiplodia gilanensis as a model. A transcriptional study revealed that the fungus is capable of using the phenylpropanoid precursors and salicylic acid to avoid the host defense response of the plant. On another hand, several genes encoding enzymes involved in different melanin synthesis pathways; 3,4-dihydroxyphenylalanine (DOPA)-melanin, 1,8-dihydroxynaphthalene (DHN)-melanin and pyomelanin; were identified and their production evauated, concluding that the fungus use diferent types of melanin to overcome environmental stress. An in silico analysis shows the presence of those genes in all the available genomes of Botryosphaeriaceae in the GeneBank, evidencing the importance of the melanin in this family. L. gilanensis produces siderophores of catechol and hydroxamate-type as well as naturally esterified fatty acids that might have a role in plant growth regulation. A plethora of hydrolytic enzymes is also produced, including xylanases, ligninases, cellulases, pectinases, cutinases, and hemicelluloses. Another secondary metabolite produced is oxalic acid, reported as a pathogenicity factor in other fungi, which role is under evaluation. Microscopical observations showed that the fungus uses the starch deposited in the ray cells as carbon source, induces the production of suberin and phenolic compounds and colonizes the vascular cambium, ray parenchyma, and the vascular bundles. These studies extend our understanding of the pathogenicity of a widely distributed pathogenic fungus with our final goal to control the diseases it causes.
Fil: Rangel Montoya Edelweiss. Consejo Nacional de Ciencia y Tecnología de México. Centro de Investigación Científica y de Educación Superior de Ensenada Baja California; México
Fil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hernández Martínez Rufina. Consejo Nacional de Ciencia y Tecnología de México. Centro de Investigación Científica y de Educación Superior de Ensenada Baja California; México
11th International Workshop on grapevine trunk diseases
Penticton
Canadá
Oregon Wine Research Institute
Materia
PCWDEs
polyphenolic
Tyrosine catabolism
melanin
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/155456
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/155456
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lasiodiplodia gilanensis used as model for understanding the pathogenicity of BotryosphaeriaceaeRangel Montoya EdelweissPaolinelli, MarcosHernández Martínez RufinaPCWDEspolyphenolicTyrosine catabolismmelaninhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Lasiodiplodia spp. cause degenerative diseases, dieback and plant death in a wide range of woody plants. To understand the fungal behavior of the genus we are using Lasiodiplodia gilanensis as a model. A transcriptional study revealed that the fungus is capable of using the phenylpropanoid precursors and salicylic acid to avoid the host defense response of the plant. On another hand, several genes encoding enzymes involved in different melanin synthesis pathways; 3,4-dihydroxyphenylalanine (DOPA)-melanin, 1,8-dihydroxynaphthalene (DHN)-melanin and pyomelanin; were identified and their production evauated, concluding that the fungus use diferent types of melanin to overcome environmental stress. An in silico analysis shows the presence of those genes in all the available genomes of Botryosphaeriaceae in the GeneBank, evidencing the importance of the melanin in this family. L. gilanensis produces siderophores of catechol and hydroxamate-type as well as naturally esterified fatty acids that might have a role in plant growth regulation. A plethora of hydrolytic enzymes is also produced, including xylanases, ligninases, cellulases, pectinases, cutinases, and hemicelluloses. Another secondary metabolite produced is oxalic acid, reported as a pathogenicity factor in other fungi, which role is under evaluation. Microscopical observations showed that the fungus uses the starch deposited in the ray cells as carbon source, induces the production of suberin and phenolic compounds and colonizes the vascular cambium, ray parenchyma, and the vascular bundles. These studies extend our understanding of the pathogenicity of a widely distributed pathogenic fungus with our final goal to control the diseases it causes.Fil: Rangel Montoya Edelweiss. Consejo Nacional de Ciencia y Tecnología de México. Centro de Investigación Científica y de Educación Superior de Ensenada Baja California; MéxicoFil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hernández Martínez Rufina. Consejo Nacional de Ciencia y Tecnología de México. Centro de Investigación Científica y de Educación Superior de Ensenada Baja California; México11th International Workshop on grapevine trunk diseasesPentictonCanadáOregon Wine Research InstituteFirenze University Press2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectWorkshopJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/155456Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae; 11th International Workshop on grapevine trunk diseases; Penticton; Canadá; 2019; 416 - 4170031-94651593-2095CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://ucanr.edu/sites/ICGTD/files/343537.pdfinfo:eu-repo/semantics/altIdentifier/doi/10.14601/Phytopathol_Mediter-10627info:eu-repo/semantics/altIdentifier/url/https://oajournals.fupress.net/index.php/pm/article/view/10627/10622Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:41:48Zoai:ri.conicet.gov.ar:11336/155456instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:41:48.356CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae
title Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae
spellingShingle Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae
Rangel Montoya Edelweiss
PCWDEs
polyphenolic
Tyrosine catabolism
melanin
title_short Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae
title_full Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae
title_fullStr Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae
title_full_unstemmed Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae
title_sort Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae
dc.creator.none.fl_str_mv Rangel Montoya Edelweiss
Paolinelli, Marcos
Hernández Martínez Rufina
author Rangel Montoya Edelweiss
author_facet Rangel Montoya Edelweiss
Paolinelli, Marcos
Hernández Martínez Rufina
author_role author
author2 Paolinelli, Marcos
Hernández Martínez Rufina
author2_role author
author
dc.subject.none.fl_str_mv PCWDEs
polyphenolic
Tyrosine catabolism
melanin
topic PCWDEs
polyphenolic
Tyrosine catabolism
melanin
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Lasiodiplodia spp. cause degenerative diseases, dieback and plant death in a wide range of woody plants. To understand the fungal behavior of the genus we are using Lasiodiplodia gilanensis as a model. A transcriptional study revealed that the fungus is capable of using the phenylpropanoid precursors and salicylic acid to avoid the host defense response of the plant. On another hand, several genes encoding enzymes involved in different melanin synthesis pathways; 3,4-dihydroxyphenylalanine (DOPA)-melanin, 1,8-dihydroxynaphthalene (DHN)-melanin and pyomelanin; were identified and their production evauated, concluding that the fungus use diferent types of melanin to overcome environmental stress. An in silico analysis shows the presence of those genes in all the available genomes of Botryosphaeriaceae in the GeneBank, evidencing the importance of the melanin in this family. L. gilanensis produces siderophores of catechol and hydroxamate-type as well as naturally esterified fatty acids that might have a role in plant growth regulation. A plethora of hydrolytic enzymes is also produced, including xylanases, ligninases, cellulases, pectinases, cutinases, and hemicelluloses. Another secondary metabolite produced is oxalic acid, reported as a pathogenicity factor in other fungi, which role is under evaluation. Microscopical observations showed that the fungus uses the starch deposited in the ray cells as carbon source, induces the production of suberin and phenolic compounds and colonizes the vascular cambium, ray parenchyma, and the vascular bundles. These studies extend our understanding of the pathogenicity of a widely distributed pathogenic fungus with our final goal to control the diseases it causes.
Fil: Rangel Montoya Edelweiss. Consejo Nacional de Ciencia y Tecnología de México. Centro de Investigación Científica y de Educación Superior de Ensenada Baja California; México
Fil: Paolinelli, Marcos. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Mendoza-San Juan. Estación Experimental Agropecuaria Mendoza; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hernández Martínez Rufina. Consejo Nacional de Ciencia y Tecnología de México. Centro de Investigación Científica y de Educación Superior de Ensenada Baja California; México
11th International Workshop on grapevine trunk diseases
Penticton
Canadá
Oregon Wine Research Institute
description Lasiodiplodia spp. cause degenerative diseases, dieback and plant death in a wide range of woody plants. To understand the fungal behavior of the genus we are using Lasiodiplodia gilanensis as a model. A transcriptional study revealed that the fungus is capable of using the phenylpropanoid precursors and salicylic acid to avoid the host defense response of the plant. On another hand, several genes encoding enzymes involved in different melanin synthesis pathways; 3,4-dihydroxyphenylalanine (DOPA)-melanin, 1,8-dihydroxynaphthalene (DHN)-melanin and pyomelanin; were identified and their production evauated, concluding that the fungus use diferent types of melanin to overcome environmental stress. An in silico analysis shows the presence of those genes in all the available genomes of Botryosphaeriaceae in the GeneBank, evidencing the importance of the melanin in this family. L. gilanensis produces siderophores of catechol and hydroxamate-type as well as naturally esterified fatty acids that might have a role in plant growth regulation. A plethora of hydrolytic enzymes is also produced, including xylanases, ligninases, cellulases, pectinases, cutinases, and hemicelluloses. Another secondary metabolite produced is oxalic acid, reported as a pathogenicity factor in other fungi, which role is under evaluation. Microscopical observations showed that the fungus uses the starch deposited in the ray cells as carbon source, induces the production of suberin and phenolic compounds and colonizes the vascular cambium, ray parenchyma, and the vascular bundles. These studies extend our understanding of the pathogenicity of a widely distributed pathogenic fungus with our final goal to control the diseases it causes.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Workshop
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/155456
Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae; 11th International Workshop on grapevine trunk diseases; Penticton; Canadá; 2019; 416 - 417
0031-9465
1593-2095
CONICET Digital
CONICET
url http://hdl.handle.net/11336/155456
identifier_str_mv Lasiodiplodia gilanensis used as model for understanding the pathogenicity of Botryosphaeriaceae; 11th International Workshop on grapevine trunk diseases; Penticton; Canadá; 2019; 416 - 417
0031-9465
1593-2095
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://ucanr.edu/sites/ICGTD/files/343537.pdf
info:eu-repo/semantics/altIdentifier/doi/10.14601/Phytopathol_Mediter-10627
info:eu-repo/semantics/altIdentifier/url/https://oajournals.fupress.net/index.php/pm/article/view/10627/10622
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Firenze University Press
publisher.none.fl_str_mv Firenze University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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