Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis

Autores
Blanco, Federico Carlos; Bianco, María Veronica; Garbaccio, Sergio Gabriel; Meikle, Virginia; Gravisaco, María José; Montenegro, Valeria Noely; Alfonseca, Edgar; Singh, Mahavir G.; Barandiaran, Soledad; Canal, Ana María; Vagnoni, Lucas Emilio; Buddle, Bryce Malcom; Bigi, Fabiana; Cataldi, Angel Adrian
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per group) were vaccinated and challenged with a virulent strain of M. bovis by the intratracheal route 9 weeks after vaccination. A non-vaccinated group and a group vaccinated with BCG were included as controls. Blood samples were collected to measure IFN-γ by an interferon-gamma release assay (IGRA), cytometry and cytokine responses of bovine purified protein derivative (PPD) restimulated peripheral blood mononuclear cells (PBMCs). The IGRA test showed IFN-γ values similar to pre-vaccination except for the animals vaccinated with M. bovis Δmce2, where a significant increase was observed at 30 days post-vaccination. The expression of IL-2R on CD4+ cells in response to PPD from the animals vaccinated with Δmce2 increased at 15 days post-vaccination compared to cells from non-vaccinated group. Vaccination of cattle with M. bovis Δmce2 induced the highest (P < 0.05) expression of IFN-γ and IL-17 mRNA upon PPD stimulation of PBMCs compared to vaccination with BCG or that for the non-vaccinated group. There was a weak positive correlation between the production of these proinflammatory cytokines post-vaccination and reduced pathology scores post-challenge. The animals were euthanized and necropsied 100 days after challenge. The group vaccinated with M. bovis Δmce2 displayed a significantly lower histopathological score for lesions in lungs and pulmonary lymph nodes than for the other groups (P < 0.05). A marked positive reaction to tuberculin intradermal test was observed post-vaccination in animals vaccinated with M. bovis Δmce2 compared to those vaccinated with BCG or the non-vaccinated group. In contrast, after challenge, non-vaccinated animals had greater skin test responses than the vaccinated animals. In summary, M. bovis Δmce2 is a promising vaccine candidate to control M. bovis pathogenesis in cattle.
Instituto de Biotecnología
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Bianco, Marí­a Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Meikle, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Gravisaco, Marí­a José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Alfonseca, Edgar. Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia; México
Fil: Singh, Mahavir G. Lionnex; Alemania
Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; Argentina
Fil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Buddle, Bryce Malcom. AgResearch. Hopkirk Research Institute; Nueva Zelanda
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Tuberculosis 93 (3) : 363-372 (May 2013)
Materia
Tuberculosis
Infección por Mycobacterium bovis
Vacuna Viva
Ganado Bovino
Organismos Patógenos Virulentos
Mycobacterium bovis Infections
Live Vaccines
Cattle
Virulent Pathogens
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8172
Acceder
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oai_identifier_str oai:localhost:20.500.12123/8172
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovisBlanco, Federico CarlosBianco, María VeronicaGarbaccio, Sergio GabrielMeikle, VirginiaGravisaco, María JoséMontenegro, Valeria NoelyAlfonseca, EdgarSingh, Mahavir G.Barandiaran, SoledadCanal, Ana MaríaVagnoni, Lucas EmilioBuddle, Bryce MalcomBigi, FabianaCataldi, Angel AdrianTuberculosisInfección por Mycobacterium bovisVacuna VivaGanado BovinoOrganismos Patógenos VirulentosMycobacterium bovis InfectionsLive VaccinesCattleVirulent PathogensA Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per group) were vaccinated and challenged with a virulent strain of M. bovis by the intratracheal route 9 weeks after vaccination. A non-vaccinated group and a group vaccinated with BCG were included as controls. Blood samples were collected to measure IFN-γ by an interferon-gamma release assay (IGRA), cytometry and cytokine responses of bovine purified protein derivative (PPD) restimulated peripheral blood mononuclear cells (PBMCs). The IGRA test showed IFN-γ values similar to pre-vaccination except for the animals vaccinated with M. bovis Δmce2, where a significant increase was observed at 30 days post-vaccination. The expression of IL-2R on CD4+ cells in response to PPD from the animals vaccinated with Δmce2 increased at 15 days post-vaccination compared to cells from non-vaccinated group. Vaccination of cattle with M. bovis Δmce2 induced the highest (P < 0.05) expression of IFN-γ and IL-17 mRNA upon PPD stimulation of PBMCs compared to vaccination with BCG or that for the non-vaccinated group. There was a weak positive correlation between the production of these proinflammatory cytokines post-vaccination and reduced pathology scores post-challenge. The animals were euthanized and necropsied 100 days after challenge. The group vaccinated with M. bovis Δmce2 displayed a significantly lower histopathological score for lesions in lungs and pulmonary lymph nodes than for the other groups (P < 0.05). A marked positive reaction to tuberculin intradermal test was observed post-vaccination in animals vaccinated with M. bovis Δmce2 compared to those vaccinated with BCG or the non-vaccinated group. In contrast, after challenge, non-vaccinated animals had greater skin test responses than the vaccinated animals. In summary, M. bovis Δmce2 is a promising vaccine candidate to control M. bovis pathogenesis in cattle.Instituto de BiotecnologíaFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Bianco, Marí­a Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Meikle, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Gravisaco, Marí­a José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Alfonseca, Edgar. Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia; MéxicoFil: Singh, Mahavir G. Lionnex; AlemaniaFil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; ArgentinaFil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Buddle, Bryce Malcom. AgResearch. Hopkirk Research Institute; Nueva ZelandaFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaElsevier2020-11-02T15:59:25Z2020-11-02T15:59:25Z2013-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8172https://www.sciencedirect.com/science/article/abs/pii/S14729792130001271472-9792https://doi.org/10.1016/j.tube.2013.02.004Tuberculosis 93 (3) : 363-372 (May 2013)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/AERG-232001/AR./Desarrollos biotecnológicos para el control de enfermedades infecciosas en animales de interés económicoinfo:eu-repograntAgreement/INTA/AEBIO-243512/AR./Búsqueda y caracterización de biomarcadores para el control de enfermedades de interés pecuario mediante el empleo de técnicas post genómicas.info:eu-repo/semantics/restrictedAccess2025-09-04T09:48:40Zoai:localhost:20.500.12123/8172instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:41.34INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis
title Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis
spellingShingle Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis
Blanco, Federico Carlos
Tuberculosis
Infección por Mycobacterium bovis
Vacuna Viva
Ganado Bovino
Organismos Patógenos Virulentos
Mycobacterium bovis Infections
Live Vaccines
Cattle
Virulent Pathogens
title_short Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis
title_full Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis
title_fullStr Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis
title_full_unstemmed Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis
title_sort Mycobacterium bovis Dmce2 double deletion mutant protects cattle against challenge with virulent M. bovis
dc.creator.none.fl_str_mv Blanco, Federico Carlos
Bianco, María Veronica
Garbaccio, Sergio Gabriel
Meikle, Virginia
Gravisaco, María José
Montenegro, Valeria Noely
Alfonseca, Edgar
Singh, Mahavir G.
Barandiaran, Soledad
Canal, Ana María
Vagnoni, Lucas Emilio
Buddle, Bryce Malcom
Bigi, Fabiana
Cataldi, Angel Adrian
author Blanco, Federico Carlos
author_facet Blanco, Federico Carlos
Bianco, María Veronica
Garbaccio, Sergio Gabriel
Meikle, Virginia
Gravisaco, María José
Montenegro, Valeria Noely
Alfonseca, Edgar
Singh, Mahavir G.
Barandiaran, Soledad
Canal, Ana María
Vagnoni, Lucas Emilio
Buddle, Bryce Malcom
Bigi, Fabiana
Cataldi, Angel Adrian
author_role author
author2 Bianco, María Veronica
Garbaccio, Sergio Gabriel
Meikle, Virginia
Gravisaco, María José
Montenegro, Valeria Noely
Alfonseca, Edgar
Singh, Mahavir G.
Barandiaran, Soledad
Canal, Ana María
Vagnoni, Lucas Emilio
Buddle, Bryce Malcom
Bigi, Fabiana
Cataldi, Angel Adrian
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Tuberculosis
Infección por Mycobacterium bovis
Vacuna Viva
Ganado Bovino
Organismos Patógenos Virulentos
Mycobacterium bovis Infections
Live Vaccines
Cattle
Virulent Pathogens
topic Tuberculosis
Infección por Mycobacterium bovis
Vacuna Viva
Ganado Bovino
Organismos Patógenos Virulentos
Mycobacterium bovis Infections
Live Vaccines
Cattle
Virulent Pathogens
dc.description.none.fl_txt_mv A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per group) were vaccinated and challenged with a virulent strain of M. bovis by the intratracheal route 9 weeks after vaccination. A non-vaccinated group and a group vaccinated with BCG were included as controls. Blood samples were collected to measure IFN-γ by an interferon-gamma release assay (IGRA), cytometry and cytokine responses of bovine purified protein derivative (PPD) restimulated peripheral blood mononuclear cells (PBMCs). The IGRA test showed IFN-γ values similar to pre-vaccination except for the animals vaccinated with M. bovis Δmce2, where a significant increase was observed at 30 days post-vaccination. The expression of IL-2R on CD4+ cells in response to PPD from the animals vaccinated with Δmce2 increased at 15 days post-vaccination compared to cells from non-vaccinated group. Vaccination of cattle with M. bovis Δmce2 induced the highest (P < 0.05) expression of IFN-γ and IL-17 mRNA upon PPD stimulation of PBMCs compared to vaccination with BCG or that for the non-vaccinated group. There was a weak positive correlation between the production of these proinflammatory cytokines post-vaccination and reduced pathology scores post-challenge. The animals were euthanized and necropsied 100 days after challenge. The group vaccinated with M. bovis Δmce2 displayed a significantly lower histopathological score for lesions in lungs and pulmonary lymph nodes than for the other groups (P < 0.05). A marked positive reaction to tuberculin intradermal test was observed post-vaccination in animals vaccinated with M. bovis Δmce2 compared to those vaccinated with BCG or the non-vaccinated group. In contrast, after challenge, non-vaccinated animals had greater skin test responses than the vaccinated animals. In summary, M. bovis Δmce2 is a promising vaccine candidate to control M. bovis pathogenesis in cattle.
Instituto de Biotecnología
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Bianco, Marí­a Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Meikle, Virginia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Gravisaco, Marí­a José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Montenegro, Valeria Noely. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Alfonseca, Edgar. Universidad Nacional Autónoma de México. Facultad de Medicina Veterinaria y Zootecnia; México
Fil: Singh, Mahavir G. Lionnex; Alemania
Fil: Barandiaran, Soledad. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; Argentina
Fil: Vagnoni, Lucas Emilio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Buddle, Bryce Malcom. AgResearch. Hopkirk Research Institute; Nueva Zelanda
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description A Mycobacterium bovis strain deleted in mce2A and mce2B genes (M. bovis Δmce2) was tested as an experimental vaccine in cattle challenged with a virulent M. bovis strain. Three-and-a-half-month old calves (n = 5 to 6 per group) were vaccinated and challenged with a virulent strain of M. bovis by the intratracheal route 9 weeks after vaccination. A non-vaccinated group and a group vaccinated with BCG were included as controls. Blood samples were collected to measure IFN-γ by an interferon-gamma release assay (IGRA), cytometry and cytokine responses of bovine purified protein derivative (PPD) restimulated peripheral blood mononuclear cells (PBMCs). The IGRA test showed IFN-γ values similar to pre-vaccination except for the animals vaccinated with M. bovis Δmce2, where a significant increase was observed at 30 days post-vaccination. The expression of IL-2R on CD4+ cells in response to PPD from the animals vaccinated with Δmce2 increased at 15 days post-vaccination compared to cells from non-vaccinated group. Vaccination of cattle with M. bovis Δmce2 induced the highest (P < 0.05) expression of IFN-γ and IL-17 mRNA upon PPD stimulation of PBMCs compared to vaccination with BCG or that for the non-vaccinated group. There was a weak positive correlation between the production of these proinflammatory cytokines post-vaccination and reduced pathology scores post-challenge. The animals were euthanized and necropsied 100 days after challenge. The group vaccinated with M. bovis Δmce2 displayed a significantly lower histopathological score for lesions in lungs and pulmonary lymph nodes than for the other groups (P < 0.05). A marked positive reaction to tuberculin intradermal test was observed post-vaccination in animals vaccinated with M. bovis Δmce2 compared to those vaccinated with BCG or the non-vaccinated group. In contrast, after challenge, non-vaccinated animals had greater skin test responses than the vaccinated animals. In summary, M. bovis Δmce2 is a promising vaccine candidate to control M. bovis pathogenesis in cattle.
publishDate 2013
dc.date.none.fl_str_mv 2013-05
2020-11-02T15:59:25Z
2020-11-02T15:59:25Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8172
https://www.sciencedirect.com/science/article/abs/pii/S1472979213000127
1472-9792
https://doi.org/10.1016/j.tube.2013.02.004
url http://hdl.handle.net/20.500.12123/8172
https://www.sciencedirect.com/science/article/abs/pii/S1472979213000127
https://doi.org/10.1016/j.tube.2013.02.004
identifier_str_mv 1472-9792
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/AERG-232001/AR./Desarrollos biotecnológicos para el control de enfermedades infecciosas en animales de interés económico
info:eu-repograntAgreement/INTA/AEBIO-243512/AR./Búsqueda y caracterización de biomarcadores para el control de enfermedades de interés pecuario mediante el empleo de técnicas post genómicas.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Tuberculosis 93 (3) : 363-372 (May 2013)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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