Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles

Autores
Bigi, María Mercedes; Vazquez, Cristina Lourdes; Castelão, Ana Beatriz C.; Garcia, Elizabeth Andrea; Cataldi, Angel Adrian; Jackson, Mary; McNeil, Michael; Soria, Marcelo Abel; Zumarraga, Martin Jose; Cabruja, Matias; Gago, Gabriela; Blanco, Federico Carlos; Nishibe, Christiane; Almeida Junior, Nalvo Franco de; Araujo, Flabio Ribeiro de; Bigi, Fabiana
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines.
Instituto de Biotecnología
Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castelão, Ana Beatriz C. Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia; Brasil
Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jackson, Mary. Colorado State University. Dept. of Microbiology, Immunology and Pathology; Estados Unidos
Fil: McNeil, Michael. Colorado State University. Dept. of Microbiology, Immunology and Pathology; Estados Unidos
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabruja, Matias. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gago, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nishibe, Christiane. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; Brasil
Fil: Almeida Junior, Nalvo Franco de. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; Brasil
Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de investigaciones Científicas y Tecnológicas; Argentina
Fuente
Veterinary microbiology 239 : 108482. (December 2019)
Materia
Mycobacterium Bovis
Genomas
Virulencia
Mutación
Patogenicidad
Bovina
Tuberculosis
Infección por Mycobacterium Bovis
Vacuna
Genomes
Virulence
Mutation
Pathogenicity
Bovinae
Mycobacterium Bovis Infections
Vaccines
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/6681
Acceder
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network_name_str INTA Digital (INTA)
spelling Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profilesBigi, María MercedesVazquez, Cristina LourdesCastelão, Ana Beatriz C.Garcia, Elizabeth AndreaCataldi, Angel AdrianJackson, MaryMcNeil, MichaelSoria, Marcelo AbelZumarraga, Martin JoseCabruja, MatiasGago, GabrielaBlanco, Federico CarlosNishibe, ChristianeAlmeida Junior, Nalvo Franco deAraujo, Flabio Ribeiro deBigi, FabianaMycobacterium BovisGenomasVirulenciaMutaciónPatogenicidadBovinaTuberculosisInfección por Mycobacterium BovisVacunaGenomesVirulenceMutationPathogenicityBovinaeMycobacterium Bovis InfectionsVaccinesMycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines.Instituto de BiotecnologíaFil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Castelão, Ana Beatriz C. Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia; BrasilFil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Jackson, Mary. Colorado State University. Dept. of Microbiology, Immunology and Pathology; Estados UnidosFil: McNeil, Michael. Colorado State University. Dept. of Microbiology, Immunology and Pathology; Estados UnidosFil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cabruja, Matias. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Gago, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nishibe, Christiane. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; BrasilFil: Almeida Junior, Nalvo Franco de. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; BrasilFil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; BrasilFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de investigaciones Científicas y Tecnológicas; ArgentinaElsevier2020-01-15T12:59:14Z2020-01-15T12:59:14Z2019-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/6681https://www.sciencedirect.com/science/article/pii/S0378113519301099?via%3Dihub0378-1135https://doi.org/10.1016/j.vetmic.2019.108482Veterinary microbiology 239 : 108482. 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dc.title.none.fl_str_mv Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles
title Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles
spellingShingle Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles
Bigi, María Mercedes
Mycobacterium Bovis
Genomas
Virulencia
Mutación
Patogenicidad
Bovina
Tuberculosis
Infección por Mycobacterium Bovis
Vacuna
Genomes
Virulence
Mutation
Pathogenicity
Bovinae
Mycobacterium Bovis Infections
Vaccines
title_short Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles
title_full Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles
title_fullStr Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles
title_full_unstemmed Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles
title_sort Analysing nonsynonymous mutations between two Mycobacterium bovis strains with contrasting pathogenic profiles
dc.creator.none.fl_str_mv Bigi, María Mercedes
Vazquez, Cristina Lourdes
Castelão, Ana Beatriz C.
Garcia, Elizabeth Andrea
Cataldi, Angel Adrian
Jackson, Mary
McNeil, Michael
Soria, Marcelo Abel
Zumarraga, Martin Jose
Cabruja, Matias
Gago, Gabriela
Blanco, Federico Carlos
Nishibe, Christiane
Almeida Junior, Nalvo Franco de
Araujo, Flabio Ribeiro de
Bigi, Fabiana
author Bigi, María Mercedes
author_facet Bigi, María Mercedes
Vazquez, Cristina Lourdes
Castelão, Ana Beatriz C.
Garcia, Elizabeth Andrea
Cataldi, Angel Adrian
Jackson, Mary
McNeil, Michael
Soria, Marcelo Abel
Zumarraga, Martin Jose
Cabruja, Matias
Gago, Gabriela
Blanco, Federico Carlos
Nishibe, Christiane
Almeida Junior, Nalvo Franco de
Araujo, Flabio Ribeiro de
Bigi, Fabiana
author_role author
author2 Vazquez, Cristina Lourdes
Castelão, Ana Beatriz C.
Garcia, Elizabeth Andrea
Cataldi, Angel Adrian
Jackson, Mary
McNeil, Michael
Soria, Marcelo Abel
Zumarraga, Martin Jose
Cabruja, Matias
Gago, Gabriela
Blanco, Federico Carlos
Nishibe, Christiane
Almeida Junior, Nalvo Franco de
Araujo, Flabio Ribeiro de
Bigi, Fabiana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium Bovis
Genomas
Virulencia
Mutación
Patogenicidad
Bovina
Tuberculosis
Infección por Mycobacterium Bovis
Vacuna
Genomes
Virulence
Mutation
Pathogenicity
Bovinae
Mycobacterium Bovis Infections
Vaccines
topic Mycobacterium Bovis
Genomas
Virulencia
Mutación
Patogenicidad
Bovina
Tuberculosis
Infección por Mycobacterium Bovis
Vacuna
Genomes
Virulence
Mutation
Pathogenicity
Bovinae
Mycobacterium Bovis Infections
Vaccines
dc.description.none.fl_txt_mv Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines.
Instituto de Biotecnología
Fil: Bigi, María Mercedes. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Castelão, Ana Beatriz C. Universidade Federal de Mato Grosso do Sul. Faculdade de Medicina Veterinária e Zootecnia; Brasil
Fil: Garcia, Elizabeth Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Jackson, Mary. Colorado State University. Dept. of Microbiology, Immunology and Pathology; Estados Unidos
Fil: McNeil, Michael. Colorado State University. Dept. of Microbiology, Immunology and Pathology; Estados Unidos
Fil: Soria, Marcelo Abel. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Biología Aplicada y Alimentos. Cátedra de Microbiología Agrícola; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cabruja, Matias. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gago, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nishibe, Christiane. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; Brasil
Fil: Almeida Junior, Nalvo Franco de. Universidade Federal de Mato Grosso do Sul. Faculdade de Computação; Brasil
Fil: Araujo, Flabio Ribeiro de. Empresa Brasileira de Pesquisa Agropecuária (Embrapa). Gado de Corte; Brasil
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de investigaciones Científicas y Tecnológicas; Argentina
description Mycobacterium bovis (M. bovis) is the causative agent of bovine tuberculosis, a chronic infectious disease that can affect cattle, other domesticated species, wild animals and humans. This disease produces important economic losses worldwide. Two M. bovis strains (04-303 and 534) have been isolated in Argentina. Whereas the 04-303 strain was isolated from a wild boar, the 534 strain was obtained from cattle. In a previous study, six weeks after infection, the 04-303 strain induced 100% mortality in mice. By contrast, mice infected with the 534 strain survived, with limited tissue damage, after four months. In this study we compared all predictive proteins encoded in both M. bovis genomes. The comparative analysis revealed 141 polymorphic proteins between both strains. From these proteins, nine virulence proteins showed polymorphisms in 04-303, whereas five did it in the 534 strain. Remarkably, both strains contained a high level of polymorphism in proteins related to phthiocerol dimycocerosate (PDIM) synthesis or transport. Further experimental evidence indicated that only mutations in the 534 strain have an impact on PDIM synthesis. The observed reduction in PDIM content in the 534 strain, together with its low capacity to induce phagosome arrest, may be associated with the reported deficiency of this strain to replicate and survive inside bovine macrophages. The findings of this study could contribute to a better understanding of pathogenicity and virulence aspects of M. bovis, which is essential for further studies aiming at developing new vaccines and diagnostic techniques for bovines.
publishDate 2019
dc.date.none.fl_str_mv 2019-12
2020-01-15T12:59:14Z
2020-01-15T12:59:14Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/6681
https://www.sciencedirect.com/science/article/pii/S0378113519301099?via%3Dihub
0378-1135
https://doi.org/10.1016/j.vetmic.2019.108482
url http://hdl.handle.net/20.500.12123/6681
https://www.sciencedirect.com/science/article/pii/S0378113519301099?via%3Dihub
https://doi.org/10.1016/j.vetmic.2019.108482
identifier_str_mv 0378-1135
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary microbiology 239 : 108482. (December 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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