Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize

Autores
Camiletti, Boris Xavier; Moral, Juan; Asensio, Claudia Mariana; Torrico Ramallo, Ada Karina; Lucini, Enrique Iván; Gimenez, Maria De La Paz; Michailides, Themis J.
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Maize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the present study, 83 A. flavus isolates from two maize regions of Argentina were characterized and evaluated for their ability to produce or lack of producing mycotoxins in order to select atoxigenic strains to be used as potential biocontrol agents (BCA). All of the isolates were tested for aflatoxin and cyclopiazonic acid (CPA) production in maize kernels and a liquid culture medium. Genetic diversity of the nonaflatoxigenic isolates was evaluated by analysis of vegetative compatibility groups (VCG) and confirmation of deletions in the aflatoxin biosynthesis cluster. Eight atoxigenic isolates were compared for their ability to reduce aflatoxin and CPA contamination in maize kernels in coinoculation tests. The A. flavus population was composed of 32% aflatoxin and CPA producers and 52% CPA producers, and 16% was determined as atoxigenic. All of the aflatoxin producer isolates also produced CPA. Aflatoxin and CPA production was significantly higher in maize kernels than in liquid medium. The 57 nonaflatoxigenic strains formed six VCG, with AM1 and AM5 being the dominant groups, with a frequency of 58 and 35%, respectively. In coinoculation experiments, all of the atoxigenic strains reduced aflatoxin from 54 to 83% and CPA from 60 to 97%. Members of group AM1 showed a greater aflatoxin reduction than members of AM5 (72 versus 66%) but no differences were detected in CPA production. Here, we described for the first time atoxigenic isolates of A. flavus that show promise to be used as BCA in maize crops in Argentina. This innovating biological control approach should be considered, developed further, and used by the maize industry to preserve the quality properties and food safety of maize kernels in Argentina.
Instituto de Patología Vegetal
Fil: Camiletti, B.X. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.
Fil: Camiletti, B.X. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Moral, Juan. University of Córdoba. Campus de Rabanales. Departamento de Agronomía; España
Fil: Moral, Juan. University of California. Kearney Agricultural Research and Extension Center; Estados Unidos
Fil: Asensio, Claudia M. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Química Biológica; Argentina
Fil: Asensio, Claudia M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Lucini, Enrique I. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Microbiología Agrícola; Argentina
Fil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Michailides, Themis J. University of California. Kearney Agricultural Research and Extension Center; Estados Unidos
Fuente
Phytopathology 108 (7) : 818-828 (2018)
Materia
Aspergillus Flavus
Zea Mays
Argentina
Aflatoxinas
Maíz
Micotoxinas
Maize
Mycotoxins
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12018

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oai_identifier_str oai:localhost:20.500.12123/12018
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spelling Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in MaizeCamiletti, Boris XavierMoral, JuanAsensio, Claudia MarianaTorrico Ramallo, Ada KarinaLucini, Enrique IvánGimenez, Maria De La PazMichailides, Themis J.Aspergillus FlavusZea MaysArgentinaAflatoxinasMaízMicotoxinasMaizeMycotoxinsMaize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the present study, 83 A. flavus isolates from two maize regions of Argentina were characterized and evaluated for their ability to produce or lack of producing mycotoxins in order to select atoxigenic strains to be used as potential biocontrol agents (BCA). All of the isolates were tested for aflatoxin and cyclopiazonic acid (CPA) production in maize kernels and a liquid culture medium. Genetic diversity of the nonaflatoxigenic isolates was evaluated by analysis of vegetative compatibility groups (VCG) and confirmation of deletions in the aflatoxin biosynthesis cluster. Eight atoxigenic isolates were compared for their ability to reduce aflatoxin and CPA contamination in maize kernels in coinoculation tests. The A. flavus population was composed of 32% aflatoxin and CPA producers and 52% CPA producers, and 16% was determined as atoxigenic. All of the aflatoxin producer isolates also produced CPA. Aflatoxin and CPA production was significantly higher in maize kernels than in liquid medium. The 57 nonaflatoxigenic strains formed six VCG, with AM1 and AM5 being the dominant groups, with a frequency of 58 and 35%, respectively. In coinoculation experiments, all of the atoxigenic strains reduced aflatoxin from 54 to 83% and CPA from 60 to 97%. Members of group AM1 showed a greater aflatoxin reduction than members of AM5 (72 versus 66%) but no differences were detected in CPA production. Here, we described for the first time atoxigenic isolates of A. flavus that show promise to be used as BCA in maize crops in Argentina. This innovating biological control approach should be considered, developed further, and used by the maize industry to preserve the quality properties and food safety of maize kernels in Argentina.Instituto de Patología VegetalFil: Camiletti, B.X. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.Fil: Camiletti, B.X. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; ArgentinaFil: Moral, Juan. University of Córdoba. Campus de Rabanales. Departamento de Agronomía; EspañaFil: Moral, Juan. University of California. Kearney Agricultural Research and Extension Center; Estados UnidosFil: Asensio, Claudia M. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Química Biológica; ArgentinaFil: Asensio, Claudia M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Lucini, Enrique I. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Microbiología Agrícola; ArgentinaFil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Michailides, Themis J. University of California. Kearney Agricultural Research and Extension Center; Estados UnidosAmerican Phytopathological Society2022-06-06T10:19:51Z2022-06-06T10:19:51Z2018-05-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12018https://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-17-0255-R0031-949Xhttps://doi.org/10.1094/PHYTO-07-17-0255-RPhytopathology 108 (7) : 818-828 (2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:23Zoai:localhost:20.500.12123/12018instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:24.769INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize
title Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize
spellingShingle Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize
Camiletti, Boris Xavier
Aspergillus Flavus
Zea Mays
Argentina
Aflatoxinas
Maíz
Micotoxinas
Maize
Mycotoxins
title_short Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize
title_full Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize
title_fullStr Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize
title_full_unstemmed Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize
title_sort Characterization of Argentinian Endemic Aspergillus flavus Isolates and Their Potential Use as Biocontrol Agents for Mycotoxins in Maize
dc.creator.none.fl_str_mv Camiletti, Boris Xavier
Moral, Juan
Asensio, Claudia Mariana
Torrico Ramallo, Ada Karina
Lucini, Enrique Iván
Gimenez, Maria De La Paz
Michailides, Themis J.
author Camiletti, Boris Xavier
author_facet Camiletti, Boris Xavier
Moral, Juan
Asensio, Claudia Mariana
Torrico Ramallo, Ada Karina
Lucini, Enrique Iván
Gimenez, Maria De La Paz
Michailides, Themis J.
author_role author
author2 Moral, Juan
Asensio, Claudia Mariana
Torrico Ramallo, Ada Karina
Lucini, Enrique Iván
Gimenez, Maria De La Paz
Michailides, Themis J.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Aspergillus Flavus
Zea Mays
Argentina
Aflatoxinas
Maíz
Micotoxinas
Maize
Mycotoxins
topic Aspergillus Flavus
Zea Mays
Argentina
Aflatoxinas
Maíz
Micotoxinas
Maize
Mycotoxins
dc.description.none.fl_txt_mv Maize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the present study, 83 A. flavus isolates from two maize regions of Argentina were characterized and evaluated for their ability to produce or lack of producing mycotoxins in order to select atoxigenic strains to be used as potential biocontrol agents (BCA). All of the isolates were tested for aflatoxin and cyclopiazonic acid (CPA) production in maize kernels and a liquid culture medium. Genetic diversity of the nonaflatoxigenic isolates was evaluated by analysis of vegetative compatibility groups (VCG) and confirmation of deletions in the aflatoxin biosynthesis cluster. Eight atoxigenic isolates were compared for their ability to reduce aflatoxin and CPA contamination in maize kernels in coinoculation tests. The A. flavus population was composed of 32% aflatoxin and CPA producers and 52% CPA producers, and 16% was determined as atoxigenic. All of the aflatoxin producer isolates also produced CPA. Aflatoxin and CPA production was significantly higher in maize kernels than in liquid medium. The 57 nonaflatoxigenic strains formed six VCG, with AM1 and AM5 being the dominant groups, with a frequency of 58 and 35%, respectively. In coinoculation experiments, all of the atoxigenic strains reduced aflatoxin from 54 to 83% and CPA from 60 to 97%. Members of group AM1 showed a greater aflatoxin reduction than members of AM5 (72 versus 66%) but no differences were detected in CPA production. Here, we described for the first time atoxigenic isolates of A. flavus that show promise to be used as BCA in maize crops in Argentina. This innovating biological control approach should be considered, developed further, and used by the maize industry to preserve the quality properties and food safety of maize kernels in Argentina.
Instituto de Patología Vegetal
Fil: Camiletti, B.X. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentina.
Fil: Camiletti, B.X. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias; Argentina
Fil: Moral, Juan. University of Córdoba. Campus de Rabanales. Departamento de Agronomía; España
Fil: Moral, Juan. University of California. Kearney Agricultural Research and Extension Center; Estados Unidos
Fil: Asensio, Claudia M. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Química Biológica; Argentina
Fil: Asensio, Claudia M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torrico Ramallo, Ada Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Lucini, Enrique I. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Microbiología Agrícola; Argentina
Fil: Gimenez, Maria De La Paz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Michailides, Themis J. University of California. Kearney Agricultural Research and Extension Center; Estados Unidos
description Maize (Zea mays L.) is a highly valuable crop in Argentina, frequently contaminated with the mycotoxins produced by Aspergillus flavus. Biocontrol products formulated with atoxigenic (nontoxic) strains of this fungal species are well known as an effective method to reduce this contamination. In the present study, 83 A. flavus isolates from two maize regions of Argentina were characterized and evaluated for their ability to produce or lack of producing mycotoxins in order to select atoxigenic strains to be used as potential biocontrol agents (BCA). All of the isolates were tested for aflatoxin and cyclopiazonic acid (CPA) production in maize kernels and a liquid culture medium. Genetic diversity of the nonaflatoxigenic isolates was evaluated by analysis of vegetative compatibility groups (VCG) and confirmation of deletions in the aflatoxin biosynthesis cluster. Eight atoxigenic isolates were compared for their ability to reduce aflatoxin and CPA contamination in maize kernels in coinoculation tests. The A. flavus population was composed of 32% aflatoxin and CPA producers and 52% CPA producers, and 16% was determined as atoxigenic. All of the aflatoxin producer isolates also produced CPA. Aflatoxin and CPA production was significantly higher in maize kernels than in liquid medium. The 57 nonaflatoxigenic strains formed six VCG, with AM1 and AM5 being the dominant groups, with a frequency of 58 and 35%, respectively. In coinoculation experiments, all of the atoxigenic strains reduced aflatoxin from 54 to 83% and CPA from 60 to 97%. Members of group AM1 showed a greater aflatoxin reduction than members of AM5 (72 versus 66%) but no differences were detected in CPA production. Here, we described for the first time atoxigenic isolates of A. flavus that show promise to be used as BCA in maize crops in Argentina. This innovating biological control approach should be considered, developed further, and used by the maize industry to preserve the quality properties and food safety of maize kernels in Argentina.
publishDate 2018
dc.date.none.fl_str_mv 2018-05-07
2022-06-06T10:19:51Z
2022-06-06T10:19:51Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12018
https://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-17-0255-R
0031-949X
https://doi.org/10.1094/PHYTO-07-17-0255-R
url http://hdl.handle.net/20.500.12123/12018
https://apsjournals.apsnet.org/doi/10.1094/PHYTO-07-17-0255-R
https://doi.org/10.1094/PHYTO-07-17-0255-R
identifier_str_mv 0031-949X
dc.language.none.fl_str_mv eng
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Phytopathological Society
publisher.none.fl_str_mv American Phytopathological Society
dc.source.none.fl_str_mv Phytopathology 108 (7) : 818-828 (2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
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repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
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