Dietary Omega-3 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy Cows

Autores: Storani, Gino; Roskopf Perez, Pablo Matías; Angeli, Emmanuel; Ribas, Lucas Emanuel; Ortega, Hugo Héctor; Salado, Eloy Eduardo; Hein, Gustavo Juan
Año de publicación: 2026
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Omega-3 (ω−3) polyunsaturated fatty acids are well recognized for their potential to influence metabolic adaptation in dairy cows during the transition period. This study evaluated the effects of calcium salts enriched with ω−3 fatty acids, including α-linolenic acid and long-chain ω−3 fatty acids such as eicosapentaenoic and docosahexaenoic acids, on hepatic lipid metabolism-related proteins, oxidative stress biomarkers, and liver function in postpartum dairy cows. Prior to calving, cows received a total mixed ration supplemented with 0.400 kg dry matter (DM)/day of calcium salts derived from a flaxseed oil–fish oil mixture (60:40). After calving, cows were fed a partial mixed ration and received 0.650 kg DM/day of the same supplement mixed with a commercial concentrate during milking. Control cows received isoenergetic diets in which calcium salts were replaced with ground corn. Blood, urine, and liver samples were collected at −21 days relative to calving (used as a baseline covariate) and at +7, and +21 (±3) days postpartum, when treatment effects were evaluated. No differences were observed between treatments in conventional biochemical markers of liver function or hepatic triacylglycerol concentration. Plasma malondialdehyde concentrations were higher in ω−3-supplemented cows, whereas other oxidative stress parameters were not affected. Supplementation with ω−3 fatty acids was associated with changes in the abundance of selected hepatic proteins involved in fatty acid metabolism, including increased acyl-CoA oxidase 1 and 3-hydroxy-3-methylglutaryl-CoA synthase and reduced diacylglycerol O-acyltransferase 1 abundance, whereas carnitine palmitoyltransferase 1 and peroxisome proliferator-activated receptor alpha abundance were not affected. These findings indicate that ω−3-enriched calcium salts induce molecular adjustments in hepatic lipid metabolism during early lactation without evidence of adverse effects on liver function.
EEA Rafaela
Fil: Storani, Gino. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Storani, Gino. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Storani, Gino. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Roskopf Perez, Pablo Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Angeli, Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Angeli, Emmanuel. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Angeli, Emmanuel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Ribas, Lucas Emanuel. Universidad Nacional del Litoral. Centro Universitario Gálvez; Argentina
Fil: Ortega, Hugo Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Salado, Eloy Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Hein, Gustavo J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Hein, Gustavo J. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina
Fil: Hein, Gustavo J. Universidad Nacional del Litoral. Centro Universitario Gálvez; Argentina
Fuente: Journal of Animal Physiology and Animal Nutrition : 1-11. (First published: 28 April 2026)
Materia: Ganado de Leche
Vacas Lecheras
Alimentación de los Animales
Ácidos Grasos
Período Perinatal
Estrés Oxidativo
Dairy Cattle
Dairy Cows
Animal Feeding
Fatty Acids
Perinatal Period
Oxidative Stress
Ácidos Grasos Omega-3
Omega-3 fatty Acids
Nivel de accesibilidad: acceso restringido
Condiciones de uso: http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/26175

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spelling	Dietary Omega-3 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy CowsStorani, GinoRoskopf Perez, Pablo MatíasAngeli, EmmanuelRibas, Lucas EmanuelOrtega, Hugo HéctorSalado, Eloy EduardoHein, Gustavo JuanGanado de LecheVacas LecherasAlimentación de los AnimalesÁcidos GrasosPeríodo PerinatalEstrés OxidativoDairy CattleDairy CowsAnimal FeedingFatty AcidsPerinatal PeriodOxidative StressÁcidos Grasos Omega-3Omega-3 fatty AcidsOmega-3 (ω−3) polyunsaturated fatty acids are well recognized for their potential to influence metabolic adaptation in dairy cows during the transition period. This study evaluated the effects of calcium salts enriched with ω−3 fatty acids, including α-linolenic acid and long-chain ω−3 fatty acids such as eicosapentaenoic and docosahexaenoic acids, on hepatic lipid metabolism-related proteins, oxidative stress biomarkers, and liver function in postpartum dairy cows. Prior to calving, cows received a total mixed ration supplemented with 0.400 kg dry matter (DM)/day of calcium salts derived from a flaxseed oil–fish oil mixture (60:40). After calving, cows were fed a partial mixed ration and received 0.650 kg DM/day of the same supplement mixed with a commercial concentrate during milking. Control cows received isoenergetic diets in which calcium salts were replaced with ground corn. Blood, urine, and liver samples were collected at −21 days relative to calving (used as a baseline covariate) and at +7, and +21 (±3) days postpartum, when treatment effects were evaluated. No differences were observed between treatments in conventional biochemical markers of liver function or hepatic triacylglycerol concentration. Plasma malondialdehyde concentrations were higher in ω−3-supplemented cows, whereas other oxidative stress parameters were not affected. Supplementation with ω−3 fatty acids was associated with changes in the abundance of selected hepatic proteins involved in fatty acid metabolism, including increased acyl-CoA oxidase 1 and 3-hydroxy-3-methylglutaryl-CoA synthase and reduced diacylglycerol O-acyltransferase 1 abundance, whereas carnitine palmitoyltransferase 1 and peroxisome proliferator-activated receptor alpha abundance were not affected. These findings indicate that ω−3-enriched calcium salts induce molecular adjustments in hepatic lipid metabolism during early lactation without evidence of adverse effects on liver function.EEA RafaelaFil: Storani, Gino. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Storani, Gino. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Storani, Gino. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Roskopf Perez, Pablo Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Angeli, Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Angeli, Emmanuel. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Angeli, Emmanuel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Ribas, Lucas Emanuel. Universidad Nacional del Litoral. Centro Universitario Gálvez; ArgentinaFil: Ortega, Hugo Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Salado, Eloy Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Hein, Gustavo J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Hein, Gustavo J. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; ArgentinaFil: Hein, Gustavo J. Universidad Nacional del Litoral. Centro Universitario Gálvez; ArgentinaWiley2026-05-13T12:58:49Z2026-05-13T12:58:49Z2026-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/26175https://onlinelibrary.wiley.com/doi/10.1111/jpn.700620931-24391439-0396https://doi.org/10.1111/jpn.70062Journal of Animal Physiology and Animal Nutrition : 1-11. (First published: 28 April 2026)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I118, Innovaciones en los procesos de crianza, recría y vaca en transición de rodeos lecherosinfo:eu-repograntAgreement/INTA/2023-PD-L01-I130, Estrategias de alimentación y nutrición para los nuevos desafíos de la producción de carnes, leches, huevos y fibrasinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-05-28T08:47:26Zoai:localhost:20.500.12123/26175instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-05-28 08:47:26.771INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Dietary Omega-3 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy Cows
title	Dietary Omega-3 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy Cows
spellingShingle	Dietary Omega-3 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy Cows Storani, Gino Ganado de Leche Vacas Lecheras Alimentación de los Animales Ácidos Grasos Período Perinatal Estrés Oxidativo Dairy Cattle Dairy Cows Animal Feeding Fatty Acids Perinatal Period Oxidative Stress Ácidos Grasos Omega-3 Omega-3 fatty Acids
title_short	Dietary Omega-3 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy Cows
title_full	Dietary Omega-3 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy Cows
title_fullStr	Dietary Omega-3 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy Cows
title_full_unstemmed	Dietary Omega-3 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy Cows
title_sort	Dietary Omega-3 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy Cows
dc.creator.none.fl_str_mv	Storani, Gino Roskopf Perez, Pablo Matías Angeli, Emmanuel Ribas, Lucas Emanuel Ortega, Hugo Héctor Salado, Eloy Eduardo Hein, Gustavo Juan
author	Storani, Gino
author_facet	Storani, Gino Roskopf Perez, Pablo Matías Angeli, Emmanuel Ribas, Lucas Emanuel Ortega, Hugo Héctor Salado, Eloy Eduardo Hein, Gustavo Juan
author_role	author
author2	Roskopf Perez, Pablo Matías Angeli, Emmanuel Ribas, Lucas Emanuel Ortega, Hugo Héctor Salado, Eloy Eduardo Hein, Gustavo Juan
author2_role	author author author author author author
dc.subject.none.fl_str_mv	Ganado de Leche Vacas Lecheras Alimentación de los Animales Ácidos Grasos Período Perinatal Estrés Oxidativo Dairy Cattle Dairy Cows Animal Feeding Fatty Acids Perinatal Period Oxidative Stress Ácidos Grasos Omega-3 Omega-3 fatty Acids
topic	Ganado de Leche Vacas Lecheras Alimentación de los Animales Ácidos Grasos Período Perinatal Estrés Oxidativo Dairy Cattle Dairy Cows Animal Feeding Fatty Acids Perinatal Period Oxidative Stress Ácidos Grasos Omega-3 Omega-3 fatty Acids
dc.description.none.fl_txt_mv	Omega-3 (ω−3) polyunsaturated fatty acids are well recognized for their potential to influence metabolic adaptation in dairy cows during the transition period. This study evaluated the effects of calcium salts enriched with ω−3 fatty acids, including α-linolenic acid and long-chain ω−3 fatty acids such as eicosapentaenoic and docosahexaenoic acids, on hepatic lipid metabolism-related proteins, oxidative stress biomarkers, and liver function in postpartum dairy cows. Prior to calving, cows received a total mixed ration supplemented with 0.400 kg dry matter (DM)/day of calcium salts derived from a flaxseed oil–fish oil mixture (60:40). After calving, cows were fed a partial mixed ration and received 0.650 kg DM/day of the same supplement mixed with a commercial concentrate during milking. Control cows received isoenergetic diets in which calcium salts were replaced with ground corn. Blood, urine, and liver samples were collected at −21 days relative to calving (used as a baseline covariate) and at +7, and +21 (±3) days postpartum, when treatment effects were evaluated. No differences were observed between treatments in conventional biochemical markers of liver function or hepatic triacylglycerol concentration. Plasma malondialdehyde concentrations were higher in ω−3-supplemented cows, whereas other oxidative stress parameters were not affected. Supplementation with ω−3 fatty acids was associated with changes in the abundance of selected hepatic proteins involved in fatty acid metabolism, including increased acyl-CoA oxidase 1 and 3-hydroxy-3-methylglutaryl-CoA synthase and reduced diacylglycerol O-acyltransferase 1 abundance, whereas carnitine palmitoyltransferase 1 and peroxisome proliferator-activated receptor alpha abundance were not affected. These findings indicate that ω−3-enriched calcium salts induce molecular adjustments in hepatic lipid metabolism during early lactation without evidence of adverse effects on liver function. EEA Rafaela Fil: Storani, Gino. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Storani, Gino. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Storani, Gino. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Roskopf Perez, Pablo Matías. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Angeli, Emmanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Angeli, Emmanuel. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Angeli, Emmanuel. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Ribas, Lucas Emanuel. Universidad Nacional del Litoral. Centro Universitario Gálvez; Argentina Fil: Ortega, Hugo Héctor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Ortega, Hugo Héctor. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Salado, Eloy Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina Fil: Hein, Gustavo J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Hein, Gustavo J. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Hein, Gustavo J. Universidad Nacional del Litoral. Centro Universitario Gálvez; Argentina
description	Omega-3 (ω−3) polyunsaturated fatty acids are well recognized for their potential to influence metabolic adaptation in dairy cows during the transition period. This study evaluated the effects of calcium salts enriched with ω−3 fatty acids, including α-linolenic acid and long-chain ω−3 fatty acids such as eicosapentaenoic and docosahexaenoic acids, on hepatic lipid metabolism-related proteins, oxidative stress biomarkers, and liver function in postpartum dairy cows. Prior to calving, cows received a total mixed ration supplemented with 0.400 kg dry matter (DM)/day of calcium salts derived from a flaxseed oil–fish oil mixture (60:40). After calving, cows were fed a partial mixed ration and received 0.650 kg DM/day of the same supplement mixed with a commercial concentrate during milking. Control cows received isoenergetic diets in which calcium salts were replaced with ground corn. Blood, urine, and liver samples were collected at −21 days relative to calving (used as a baseline covariate) and at +7, and +21 (±3) days postpartum, when treatment effects were evaluated. No differences were observed between treatments in conventional biochemical markers of liver function or hepatic triacylglycerol concentration. Plasma malondialdehyde concentrations were higher in ω−3-supplemented cows, whereas other oxidative stress parameters were not affected. Supplementation with ω−3 fatty acids was associated with changes in the abundance of selected hepatic proteins involved in fatty acid metabolism, including increased acyl-CoA oxidase 1 and 3-hydroxy-3-methylglutaryl-CoA synthase and reduced diacylglycerol O-acyltransferase 1 abundance, whereas carnitine palmitoyltransferase 1 and peroxisome proliferator-activated receptor alpha abundance were not affected. These findings indicate that ω−3-enriched calcium salts induce molecular adjustments in hepatic lipid metabolism during early lactation without evidence of adverse effects on liver function.
publishDate	2026
dc.date.none.fl_str_mv	2026-05-13T12:58:49Z 2026-05-13T12:58:49Z 2026-04
dc.type.none.fl_str_mv	info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo
format	article
status_str	publishedVersion
dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/26175 https://onlinelibrary.wiley.com/doi/10.1111/jpn.70062 0931-2439 1439-0396 https://doi.org/10.1111/jpn.70062
url	http://hdl.handle.net/20.500.12123/26175 https://onlinelibrary.wiley.com/doi/10.1111/jpn.70062 https://doi.org/10.1111/jpn.70062
identifier_str_mv	0931-2439 1439-0396
dc.language.none.fl_str_mv	eng
language	eng
dc.relation.none.fl_str_mv	info:eu-repograntAgreement/INTA/2023-PD-L01-I118, Innovaciones en los procesos de crianza, recría y vaca en transición de rodeos lecheros info:eu-repograntAgreement/INTA/2023-PD-L01-I130, Estrategias de alimentación y nutrición para los nuevos desafíos de la producción de carnes, leches, huevos y fibras
dc.rights.none.fl_str_mv	info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv	restrictedAccess
rights_invalid_str_mv	http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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dc.publisher.none.fl_str_mv	Wiley
publisher.none.fl_str_mv	Wiley
dc.source.none.fl_str_mv	Journal of Animal Physiology and Animal Nutrition : 1-11. (First published: 28 April 2026) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str	INTA Digital (INTA)
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instname_str	Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv	INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv	tripaldi.nicolas@inta.gob.ar
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Dietary Omega-3 Fatty Acids Alter Hepatic Lipid Oxidation and Oxidative Stress Markers in Postpartum Dairy Cows

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